GenBank-Updates@genbank.bio.net (01/17/91)
LOCUS BSURFOLA 4880 bp ds-DNA BCT 17-JAN-1991
DEFINITION B.subtilis folic acid biosynthetic operon encoding
para-aminobenzoic acid synthase, component I (pab), glutamine
amidotransferase (trpG), pot. p-aminobenzoate synthesis enzyme
(pabC), and pot. dihydropteroate synthase (sul) genes, complete
cds.
ACCESSION M34053
KEYWORDS H2-pteridine-CH2OH pyrophosphokinase; dihydropteroate synthase;
glutamine amidotransferase; para-aminobenzoic acid synthase.
SOURCE B.subtilis (strain VB157) vegetative cell DNA, clones pJS[1,2].
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 4880)
AUTHORS Slock,J., Stahly,D.P., Han,C.-Y., Six,E.W. and Crawford,I.P.
TITLE An apparent Bacillus subtilis folic acid biosynthetic operon
containing pab, an amphibolic trpG gene, a third gene required for
synthesis of para-aminobenzoic acid, and the dihydropteroate
synthase gene
JOURNAL J. Bacteriol. 172, 7211-7226 (1991)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by D.P.Stahly, 07-MAY-1990.
Author address: D.P.Stahly
University of Iowa
Dept. of Microbiology
Iowa City, IA 52242
e-mail: cmdsix@vaxa.weeg.uiowa.edu
FEATURES Location/Qualifiers
misc_binding 300..303
/note="ribosome binding site"
CDS 313..1725
/note="para-aminobenzoic acid synthase, component I (pab)"
misc_binding 1720..1728
/note="methyltryptophan resistance protein binding site"
misc_binding 1724..1730
/note="ribosome binding site"
CDS 1739..2323
/note="glutamine amidotransferase (trpG)"
misc_binding 2306..2313
/note="ribosome binding site"
CDS 2323..3204
/note="pot. p-aminobenzoate synthesis enzyme (pabC)"
misc_binding 3175..3179
/note="ribosome binding site"
CDS 3186..4043
/note="pot. dihydropteroate synthase (sul) (EC 2.5.1.15)"
misc_binding 4023..4027
/note="ribosome binding site"
CDS 4036..4398
/note="ORF2"
misc_binding 4381..4385
/note="ribosome binding site"
BASE COUNT 1512 a 939 c 1205 g 1224 t
ORIGIN
1 gaattctcgg aggcatctca tcaggtgcgg caatttatgc tgctcttcaa gtagcaaaaa
61 aactcggaaa aggcaaaaaa gtattggcca tcattccaag taacggcgaa cgttatctaa
121 gcacgccgct gtaccaattc gattaaaaaa agccaaaact cccggttcgc cgggagtttt
181 tttatatttc gtgcatcaaa tatgccatgt ctgcgacgaa tgttattctt ggaagagaaa
241 ttcacttttt tcactaacaa cattgcttta caattaaaaa caagtaaaaa agaaaataga
301 ggaatgatac aaatggcaca acgcagaccg gcaggcaaaa aaataccttt tcaaaaagac
361 tcattcttac aacaatttga gaaacttgcg caatcccgga aacatcatgt acttctcgaa
421 agtgcaagag gcggcagata tagtatagcc ggtcttgatc caattgcgac tgtgaaagga
481 aaagacggaa taactacaat taagcatggt gatgagatgc tgtttaaaga aggtgatcca
541 ttacgggcct tccacagctg gtttaaaaca ctggaaacag aaacgaatca tgagttccct
601 gactttcaag gcggggcaat cgggtttctc agctatgatt acgcacggta cattgaaaat
661 tttaaaatgc tctcattaga tgatttagaa acaccagata tttattttct tgtttttgat
721 gatatagcag tttatgacca tcaagaagag tctctatggc tgattactca tgttaatggt
781 tctgatcagg aaacagcgga tgtgaagcta tctgagttag agcagatgtg gttgactgag
841 cttcccgctg tcacttcgcg agagatgaag cctgaaacag ctggttcttt cgcggcgcca
901 tttaccgagg atgggttctc acaagctgta gagaaaatca aacaatacat tgccagcgga
961 gatgtgtttc aagtcaatct atcaataagg cagtcacagt cactgtctgt ccacccatat
1021 caaatttaca aaaccttgag agaagtaaat ccttctcctt atatggcgta tttagaaaca
1081 cctgatttcc aaatcatttg cggatcgcct gaactgcttg tcagcaaaaa gggcaagcta
1141 ttagagacga gaccgattgc gggcacccgt tccagaggga aaacaaatga agaagacgag
1201 gcgcttgcaa acgaattgat acacaatgaa aaagaacgcg cggaacatgt catgctggtt
1261 gatcttgagc gaaatgatct gggaagagta tcacgttacg ggtctgtgcg cgtaaatgaa
1321 ttcatggcaa ttgaaaaata ctcgcatgtg atgcacattg tgtctaatgt ccaaggtgaa
1381 ctgcaggatg ggtatgatgc tgtagatatt attcatgctg tgtttcccgg aggaaccatt
1441 actggtgcac cgaaagtaag aacgatggaa attatagaag aacttgagcc gacacgccga
1501 gggctttata ctggatctat aggatggttt ggatataatc acgatctgca gtttaatatc
1561 gtcattcgaa ccatttatgc aaccggaggg caggcattta tgcagtccgg tgcaggagtt
1621 gtgattgatt ctgttccgaa gcacgaatac aaggaatcat tcaaaaaagc ttttgcgatg
1681 caaagagcat tagagctgag cgaagaagag acaaaaatta gatgaggtga gcggagaaat
1741 gattttaatg attgataact acgattcatt cacgtacaac ttggtacagt atttgggcga
1801 gcttggggaa gagctggttg tgaaacgcaa tgacagcatc acaatcgatg aaattgaaga
1861 actgtctccg gactttctga tgatatctcc cggaccgtgc agccctgatg aggcgggaat
1921 cagcctcgaa gcaattaaac atttcgcagg gaaaattcct attttcggtg tatgtctcgg
1981 acatcagtcc atcgcacaag tgttcggtgg tgatgttgtt agggcagaac ggcttatgca
2041 cgggaaaacc tcggatatcg agcatgacgg caaaaccatt tttgaagggt tgaaaaatcc
2101 ccttgttgcg acgcgatacc actcgctgat cgtaaaacct gagacgctgc caagctgttt
2161 tacagtaaca gcacaaacga aagaaggaga aatcatggct attcgccaca atgacctccc
2221 gatagagggt gtgcaatttc acccagagtc tattatgacc tcctttggga aagaaatgct
2281 cagaaatttt attgagacat atcgcaagga agttattgcg tgatgatata tgtgaacggc
2341 cggtatatgg aggagaaaga tgcagttctt tctccttttg accatgggtt tttatatggg
2401 atcggtgttt tcgaaacctt caggctctac gaaggctgcc cgtttttgct tgattggcat
2461 atagagaggc ttgaacgcgc gcttaaggat ctgcaaatcg aatatactgt ttcaaagcat
2521 gagatccttg agatgctgga caagctgctt aagctaaatg atatcaagga tggcaatgcc
2581 cgcgtcagac tgaacatatc agcgggaatc agcgataaag gctttgtcgc ccagacgtac
2641 gataagccca ccgttttatg ctttgtgaat cagctgaaac cagaaagtct tcctttgcaa
2701 aaagaaggaa aggttttatc gatacggaga aacactccag agggatcgtt ccggttaaaa
2761 tctcatcatt atctaaataa tatgtatgcg aaacgggaaa ttggaaatga tccgcgtgtc
2821 gagggaattt tcttaacgga ggatggagct gttgcagagg gtatcatatc taatgtcttc
2881 tggagaaaag gtcgctgtat ctatacgcca tcgcttgata ccggtattct ggacggcgtc
2941 acgagacgtt ttatcataga aaatgcaaag gatatcggcc tggagctcaa aacgggccga
3001 tatgagctag aagctctctt gacagctgac gaagcgtgga tgacgaactc ggttcttgaa
3061 atcatcccgt ttactaaaat agaagaagta aactatggaa gccaaagcgg agaagcaacc
3121 tccgctcttc aattgttata caaaaaagaa ataaagaaca tgattcatga aaagggagga
3181 agagcatggc gcagcacaca atagatcaaa cacaagtaat ccacactaag cccagcgctt
3241 tatcatataa agagaagacg ctggtgatgg gaattttaaa cgtaacgcct gactctttct
3301 cggacggcgg aaaatatgac agcttggaca aggcgctgct gcacgcgaaa gagatgatcg
3361 atgatggtgc ccatatcatt gatattggag gggaatcgac aaggcctggc gctgagtgcg
3421 tatctgagga tgaggagatg tccagagtca ttccggtgat tgagcggatt acgaaagagc
3481 ttggtgttcc tatttctgta gacacgtaca aggcttctgt cgcagatgaa gcagtgaaag
3541 ccggtgcatc cattatcaat gatatttggg gagccaaaca tgatccgaag atggcttccg
3601 ttgcagctga acataatgtt ccaattgtac tcatgcataa ccgccctgaa agaaactaca
3661 atgacttatt gccggatatg ctgtcggact taatggagag tgtaaaaatt gctgttgagg
3721 ccggagtaga cgagaagaac attattcttg atcctggtat cggtttcccg aaaacctatc
3781 acgataactt ggcagtgatg aacaaactag agattttcag cggattggga tatccggttc
3841 ttctggcaac ctcccgaaaa agattcatcg gacgtgttct ggatcttccg cctgaggagc
3901 gggctgaggg cacaggcgcg actgtgtgtc tcggcattca aaaaggctgt gacattgtca
3961 gggtccatga tgtaaagcaa attgccagaa tggcgaaaat gatggacgcg atgctgaata
4021 agggaggggt gcaccatgga taaagtttat gtagaaggta tggagtttta cggatatcac
4081 ggtgtgttca cagaagaaaa caaacttggc cagcggttta aagtcgattt aaccgctgag
4141 ctggatttaa gcaaagctgg acagacagac gaccttgagc aaacgatcaa ctatgctgag
4201 ctctatcacg tatgtaaaga tatcgtggaa ggggagcctg tgaaattggt ggaaacgctg
4261 gcggaacgta ttgctggcac tgttctcgga aaatttcagc ctgttcagca atgtacggtg
4321 aaagtgatta agccagaccc gccaattccc ggacactata aatcagtagc aattgaaatt
4381 acgagaaaaa agtcatgaac aacatagctt atattgcact tggatctaat attggagata
4441 gagaaacgta tttaaggcaa gcagtggctt tactgcatca gcatgctgcg gtgacagtca
4501 ctaaagtgtc gtctatttac gaaactgacc cggtcggata cgaagatcaa gctcaatttt
4561 tgaatatggc tgttgaaatc aagacatcat tgaacccttt tgaactcctt gaactgacgc
4621 agcagataga aaatgaatta ggcagaacaa gggaagtaag atggggaccg cggacggcag
4681 accttgacat tttgttattt aatcgtgaaa atattgaaac agagcaacta attgttccgc
4741 atccgagaat gtatgagcgt ttgtttgtcc ttgcgccgct tgcggaaatt tgccagcagg
4801 ttgaaaaaga ggctacaagc gccgaaacag accaagaagg tgtaagagta tggaagcaga
4861 aatctggggt agacgaattc
//GenBank-Updates@genbank.bio.net (03/14/91)
LOCUS BSURFOLA 4880 bp ds-DNA BCT 14-MAR-1991
DEFINITION B.subtilis folic acid biosynthetic operon encoding
para-aminobenzoic acid synthase, component I (pab), glutamine
amidotransferase (trpG), pot. p-aminobenzoate synthesis enzyme
(pabC), and pot. dihydropteroate synthase (sul) genes, complete
cds.
ACCESSION M34053
KEYWORDS H2-pteridine-CH2OH pyrophosphokinase; dihydropteroate synthase;
glutamine amidotransferase; para-aminobenzoic acid synthase.
SOURCE B.subtilis (strain VB157) vegetative cell DNA, clones pJS[1,2].
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 4880)
AUTHORS Slock,J., Stahly,D.P., Han,C.-Y., Six,E.W. and Crawford,I.P.
TITLE An apparent Bacillus subtilis folic acid biosynthetic operon
containing pab, an amphibolic trpG gene, a third gene required for
synthesis of para-aminobenzoic acid, and the dihydropteroate
synthase gene
JOURNAL J. Bacteriol. 172, 7211-7226 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by D.P.Stahly, 07-MAY-1990.
Author address: D.P.Stahly
University of Iowa
Dept. of Microbiology
Iowa City, IA 52242
e-mail: cmdsix@vaxa.weeg.uiowa.edu
FEATURES Location/Qualifiers
misc_binding 300..303
/note="ribosome binding site"
CDS 313..1725
/note="para-aminobenzoic acid synthase, component I (pab)"
/codon_start=313
misc_binding 1720..1728
/note="methyltryptophan resistance protein binding site"
misc_binding 1724..1730
/note="ribosome binding site"
CDS 1739..2323
/note="glutamine amidotransferase (trpG)"
/codon_start=1739
misc_binding 2306..2313
/note="ribosome binding site"
CDS 2323..3204
/note="pot. p-aminobenzoate synthesis enzyme (pabC)"
/codon_start=2323
misc_binding 3175..3179
/note="ribosome binding site"
CDS 3186..4043
/note="pot. dihydropteroate synthase (sul) (EC 2.5.1.15)"
/codon_start=3186
misc_binding 4023..4027
/note="ribosome binding site"
CDS 4036..4398
/note="ORF1"
/codon_start=4036
misc_binding 4381..4385
/note="ribosome binding site"
BASE COUNT 1512 a 939 c 1205 g 1224 t
ORIGIN
1 gaattctcgg aggcatctca tcaggtgcgg caatttatgc tgctcttcaa gtagcaaaaa
61 aactcggaaa aggcaaaaaa gtattggcca tcattccaag taacggcgaa cgttatctaa
121 gcacgccgct gtaccaattc gattaaaaaa agccaaaact cccggttcgc cgggagtttt
181 tttatatttc gtgcatcaaa tatgccatgt ctgcgacgaa tgttattctt ggaagagaaa
241 ttcacttttt tcactaacaa cattgcttta caattaaaaa caagtaaaaa agaaaataga
301 ggaatgatac aaatggcaca acgcagaccg gcaggcaaaa aaataccttt tcaaaaagac
361 tcattcttac aacaatttga gaaacttgcg caatcccgga aacatcatgt acttctcgaa
421 agtgcaagag gcggcagata tagtatagcc ggtcttgatc caattgcgac tgtgaaagga
481 aaagacggaa taactacaat taagcatggt gatgagatgc tgtttaaaga aggtgatcca
541 ttacgggcct tccacagctg gtttaaaaca ctggaaacag aaacgaatca tgagttccct
601 gactttcaag gcggggcaat cgggtttctc agctatgatt acgcacggta cattgaaaat
661 tttaaaatgc tctcattaga tgatttagaa acaccagata tttattttct tgtttttgat
721 gatatagcag tttatgacca tcaagaagag tctctatggc tgattactca tgttaatggt
781 tctgatcagg aaacagcgga tgtgaagcta tctgagttag agcagatgtg gttgactgag
841 cttcccgctg tcacttcgcg agagatgaag cctgaaacag ctggttcttt cgcggcgcca
901 tttaccgagg atgggttctc acaagctgta gagaaaatca aacaatacat tgccagcgga
961 gatgtgtttc aagtcaatct atcaataagg cagtcacagt cactgtctgt ccacccatat
1021 caaatttaca aaaccttgag agaagtaaat ccttctcctt atatggcgta tttagaaaca
1081 cctgatttcc aaatcatttg cggatcgcct gaactgcttg tcagcaaaaa gggcaagcta
1141 ttagagacga gaccgattgc gggcacccgt tccagaggga aaacaaatga agaagacgag
1201 gcgcttgcaa acgaattgat acacaatgaa aaagaacgcg cggaacatgt catgctggtt
1261 gatcttgagc gaaatgatct gggaagagta tcacgttacg ggtctgtgcg cgtaaatgaa
1321 ttcatggcaa ttgaaaaata ctcgcatgtg atgcacattg tgtctaatgt ccaaggtgaa
1381 ctgcaggatg ggtatgatgc tgtagatatt attcatgctg tgtttcccgg aggaaccatt
1441 actggtgcac cgaaagtaag aacgatggaa attatagaag aacttgagcc gacacgccga
1501 gggctttata ctggatctat aggatggttt ggatataatc acgatctgca gtttaatatc
1561 gtcattcgaa ccatttatgc aaccggaggg caggcattta tgcagtccgg tgcaggagtt
1621 gtgattgatt ctgttccgaa gcacgaatac aaggaatcat tcaaaaaagc ttttgcgatg
1681 caaagagcat tagagctgag cgaagaagag acaaaaatta gatgaggtga gcggagaaat
1741 gattttaatg attgataact acgattcatt cacgtacaac ttggtacagt atttgggcga
1801 gcttggggaa gagctggttg tgaaacgcaa tgacagcatc acaatcgatg aaattgaaga
1861 actgtctccg gactttctga tgatatctcc cggaccgtgc agccctgatg aggcgggaat
1921 cagcctcgaa gcaattaaac atttcgcagg gaaaattcct attttcggtg tatgtctcgg
1981 acatcagtcc atcgcacaag tgttcggtgg tgatgttgtt agggcagaac ggcttatgca
2041 cgggaaaacc tcggatatcg agcatgacgg caaaaccatt tttgaagggt tgaaaaatcc
2101 ccttgttgcg acgcgatacc actcgctgat cgtaaaacct gagacgctgc caagctgttt
2161 tacagtaaca gcacaaacga aagaaggaga aatcatggct attcgccaca atgacctccc
2221 gatagagggt gtgcaatttc acccagagtc tattatgacc tcctttggga aagaaatgct
2281 cagaaatttt attgagacat atcgcaagga agttattgcg tgatgatata tgtgaacggc
2341 cggtatatgg aggagaaaga tgcagttctt tctccttttg accatgggtt tttatatggg
2401 atcggtgttt tcgaaacctt caggctctac gaaggctgcc cgtttttgct tgattggcat
2461 atagagaggc ttgaacgcgc gcttaaggat ctgcaaatcg aatatactgt ttcaaagcat
2521 gagatccttg agatgctgga caagctgctt aagctaaatg atatcaagga tggcaatgcc
2581 cgcgtcagac tgaacatatc agcgggaatc agcgataaag gctttgtcgc ccagacgtac
2641 gataagccca ccgttttatg ctttgtgaat cagctgaaac cagaaagtct tcctttgcaa
2701 aaagaaggaa aggttttatc gatacggaga aacactccag agggatcgtt ccggttaaaa
2761 tctcatcatt atctaaataa tatgtatgcg aaacgggaaa ttggaaatga tccgcgtgtc
2821 gagggaattt tcttaacgga ggatggagct gttgcagagg gtatcatatc taatgtcttc
2881 tggagaaaag gtcgctgtat ctatacgcca tcgcttgata ccggtattct ggacggcgtc
2941 acgagacgtt ttatcataga aaatgcaaag gatatcggcc tggagctcaa aacgggccga
3001 tatgagctag aagctctctt gacagctgac gaagcgtgga tgacgaactc ggttcttgaa
3061 atcatcccgt ttactaaaat agaagaagta aactatggaa gccaaagcgg agaagcaacc
3121 tccgctcttc aattgttata caaaaaagaa ataaagaaca tgattcatga aaagggagga
3181 agagcatggc gcagcacaca atagatcaaa cacaagtaat ccacactaag cccagcgctt
3241 tatcatataa agagaagacg ctggtgatgg gaattttaaa cgtaacgcct gactctttct
3301 cggacggcgg aaaatatgac agcttggaca aggcgctgct gcacgcgaaa gagatgatcg
3361 atgatggtgc ccatatcatt gatattggag gggaatcgac aaggcctggc gctgagtgcg
3421 tatctgagga tgaggagatg tccagagtca ttccggtgat tgagcggatt acgaaagagc
3481 ttggtgttcc tatttctgta gacacgtaca aggcttctgt cgcagatgaa gcagtgaaag
3541 ccggtgcatc cattatcaat gatatttggg gagccaaaca tgatccgaag atggcttccg
3601 ttgcagctga acataatgtt ccaattgtac tcatgcataa ccgccctgaa agaaactaca
3661 atgacttatt gccggatatg ctgtcggact taatggagag tgtaaaaatt gctgttgagg
3721 ccggagtaga cgagaagaac attattcttg atcctggtat cggtttcccg aaaacctatc
3781 acgataactt ggcagtgatg aacaaactag agattttcag cggattggga tatccggttc
3841 ttctggcaac ctcccgaaaa agattcatcg gacgtgttct ggatcttccg cctgaggagc
3901 gggctgaggg cacaggcgcg actgtgtgtc tcggcattca aaaaggctgt gacattgtca
3961 gggtccatga tgtaaagcaa attgccagaa tggcgaaaat gatggacgcg atgctgaata
4021 agggaggggt gcaccatgga taaagtttat gtagaaggta tggagtttta cggatatcac
4081 ggtgtgttca cagaagaaaa caaacttggc cagcggttta aagtcgattt aaccgctgag
4141 ctggatttaa gcaaagctgg acagacagac gaccttgagc aaacgatcaa ctatgctgag
4201 ctctatcacg tatgtaaaga tatcgtggaa ggggagcctg tgaaattggt ggaaacgctg
4261 gcggaacgta ttgctggcac tgttctcgga aaatttcagc ctgttcagca atgtacggtg
4321 aaagtgatta agccagaccc gccaattccc ggacactata aatcagtagc aattgaaatt
4381 acgagaaaaa agtcatgaac aacatagctt atattgcact tggatctaat attggagata
4441 gagaaacgta tttaaggcaa gcagtggctt tactgcatca gcatgctgcg gtgacagtca
4501 ctaaagtgtc gtctatttac gaaactgacc cggtcggata cgaagatcaa gctcaatttt
4561 tgaatatggc tgttgaaatc aagacatcat tgaacccttt tgaactcctt gaactgacgc
4621 agcagataga aaatgaatta ggcagaacaa gggaagtaag atggggaccg cggacggcag
4681 accttgacat tttgttattt aatcgtgaaa atattgaaac agagcaacta attgttccgc
4741 atccgagaat gtatgagcgt ttgtttgtcc ttgcgccgct tgcggaaatt tgccagcagg
4801 ttgaaaaaga ggctacaagc gccgaaacag accaagaagg tgtaagagta tggaagcaga
4861 aatctggggt agacgaattc
//