GenBank-Updates@genbank.bio.net (02/19/91)
LOCUS ECODDLA 1687 bp ds-DNA BCT 19-FEB-1991
DEFINITION E.coli D-alanine:D-alanine ligase A (ddlA) gene, complete cds.
ACCESSION M58467 J05319
KEYWORDS D-alanine:D-alanine ligase A.
SOURCE E.coli (strain W3110) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1687)
AUTHORS Zawadzke,L.E., Bugg,T.D. and Walsh,C.T.
TITLE Existence of two D-alanine:D-alanine ligases in Escherichia coli:
Cloning and sequencing of the ddlA gene, and purification and
characterisation of the DdlA and DdlB enzymes
JOURNAL Biochemistry (1990) In press
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 431..1525
/EC_number="6.3.2.4"
/product="D-alanine:D-alanine ligase A"
/gene="ddlA"
/codon_start=431
BASE COUNT 455 a 391 c 392 g 449 t
ORIGIN
1 gttaacatta tgtttcacaa cttcatagtg tctggaagta ttatttttat gacaaaggaa
61 atactttgcg caaatatttt ttgtttgatt accagcgaaa cgtttctctt atttaaataa
121 ttacccacac aaaatatagg cttttaatta tcaaccttta ttataaataa aagttatgaa
181 atatattctc ttcgttaact tataattaag agaaaaaaac tcaccgcatg gccatttaag
241 ccaggaataa attagcttcc cgttcatcat ttttgtgatc ttgcgcacat ctttgagata
301 acgcactttc ttcaccagta cgttgcacgc tttgtcagca gaagattgca acaatctttc
361 agaaggccgc gaatcccccg tttgtcgtgg taatgtatgc actttgtgtt agacgggata
421 ggtttttaag atggaaaaac tgcgggtagg aatcgttttt ggtggtaaat cagcggaaca
481 tgaagtgtct ctgcaatcgg caaaaaacat tgtcgatgcc attgataaaa gtcgcttcga
541 cgttgtgctg ctgggcattg ataaacaagg gcaatggcac gtcagcgatg ccagcaatta
601 tctgctaaat gcagacgatc ctgcccatat tgcgttgcgc ccttcggcga ccagccttgc
661 gcaggtgcca ggtaaacatg agcatcagct tatcgacgcg caaaacggtc agccgttgcc
721 gacggtggat gtcattttcc cgattgtcca cggtacgctg ggcgaagatg gttccttgca
781 gggaatgctg cgggtcgcca atttaccgtt tgtaggttct gatgttctgg cttcagcagc
841 ctgtatggac aaagatgtca ccaaacgtct gctacgcgat gccgggctga acattgcgcc
901 atttattacc ctgacgcgcg ccaatcgtca caacattagt tttgccgaag tggagtctaa
961 actggggtta ccactgtttg taaaaccggc taatcagggc tcttctgttg gtgtcagcaa
1021 agtaaccagt gaagaacagt acgcaattgc cgtcgatctg gcgttcgagt tcgatcataa
1081 agtgatcgtt gagcaaggga tcaaaggtcg tgagatcgaa tgcgcagttc tgggcaacga
1141 caatccgcaa gccagcacct gtggagagat cgtactcacc agcgatttct atgcctacga
1201 caccaagtac attgacgaag atggcgcgaa agtggtagtt ccggcagcca ttgcgccaga
1261 aatcaacgat aagatccggg cgattgccgt tcaggcttat caaacgttgg gatgcgcagg
1321 catggcgcgt gtagacgtgt ttttaacccc agagaacgaa gtggtgatca acgagatcaa
1381 caccctgcct ggcttcacta atatcagtat gtatccgaag ctgtggcaag ccagcggtct
1441 gggttacacc gatctgatca cacgtttgat tgaactggcg ctggagcgtc acgctgcgga
1501 taacgcattg aaaaccacaa tgtaatattt taacgcccga taatattctt attgggcgtt
1561 ttcagtttct tcatcgcgac gacgaataat caaccccgcc agccagaaac taatcaccca
1621 cgtcaccaga ccgacggcat aggtctgcca accttttgct tcaaatccag taacccacca
1681 cgccatt
//GenBank-Updates@genbank.bio.net (03/14/91)
LOCUS ECODDLA 1687 bp ds-DNA BCT 14-MAR-1991
DEFINITION E.coli D-alanine:D-alanine ligase A (ddlA) gene, complete cds.
ACCESSION M58467 J05319
KEYWORDS D-alanine:D-alanine ligase A.
SOURCE E.coli (strain W3110) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1687)
AUTHORS Zawadzke,L.E., Bugg,T.D. and Walsh,C.T.
TITLE Existence of Two D-alanin:D-alanine ligases in escherichia coli:
cloning and sequenceing of the ddlA gene and purificaiton and
characterization of the Dd1A and Dd1b enzymes
JOURNAL Biochemistry 30, 1673-1682 (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 431..1525
/EC_number="6.3.2.4"
/product="D-alanine:D-alanine ligase A"
/gene="ddlA"
/codon_start=431
BASE COUNT 455 a 391 c 392 g 449 t
ORIGIN
1 gttaacatta tgtttcacaa cttcatagtg tctggaagta ttatttttat gacaaaggaa
61 atactttgcg caaatatttt ttgtttgatt accagcgaaa cgtttctctt atttaaataa
121 ttacccacac aaaatatagg cttttaatta tcaaccttta ttataaataa aagttatgaa
181 atatattctc ttcgttaact tataattaag agaaaaaaac tcaccgcatg gccatttaag
241 ccaggaataa attagcttcc cgttcatcat ttttgtgatc ttgcgcacat ctttgagata
301 acgcactttc ttcaccagta cgttgcacgc tttgtcagca gaagattgca acaatctttc
361 agaaggccgc gaatcccccg tttgtcgtgg taatgtatgc actttgtgtt agacgggata
421 ggtttttaag atggaaaaac tgcgggtagg aatcgttttt ggtggtaaat cagcggaaca
481 tgaagtgtct ctgcaatcgg caaaaaacat tgtcgatgcc attgataaaa gtcgcttcga
541 cgttgtgctg ctgggcattg ataaacaagg gcaatggcac gtcagcgatg ccagcaatta
601 tctgctaaat gcagacgatc ctgcccatat tgcgttgcgc ccttcggcga ccagccttgc
661 gcaggtgcca ggtaaacatg agcatcagct tatcgacgcg caaaacggtc agccgttgcc
721 gacggtggat gtcattttcc cgattgtcca cggtacgctg ggcgaagatg gttccttgca
781 gggaatgctg cgggtcgcca atttaccgtt tgtaggttct gatgttctgg cttcagcagc
841 ctgtatggac aaagatgtca ccaaacgtct gctacgcgat gccgggctga acattgcgcc
901 atttattacc ctgacgcgcg ccaatcgtca caacattagt tttgccgaag tggagtctaa
961 actggggtta ccactgtttg taaaaccggc taatcagggc tcttctgttg gtgtcagcaa
1021 agtaaccagt gaagaacagt acgcaattgc cgtcgatctg gcgttcgagt tcgatcataa
1081 agtgatcgtt gagcaaggga tcaaaggtcg tgagatcgaa tgcgcagttc tgggcaacga
1141 caatccgcaa gccagcacct gtggagagat cgtactcacc agcgatttct atgcctacga
1201 caccaagtac attgacgaag atggcgcgaa agtggtagtt ccggcagcca ttgcgccaga
1261 aatcaacgat aagatccggg cgattgccgt tcaggcttat caaacgttgg gatgcgcagg
1321 catggcgcgt gtagacgtgt ttttaacccc agagaacgaa gtggtgatca acgagatcaa
1381 caccctgcct ggcttcacta atatcagtat gtatccgaag ctgtggcaag ccagcggtct
1441 gggttacacc gatctgatca cacgtttgat tgaactggcg ctggagcgtc acgctgcgga
1501 taacgcattg aaaaccacaa tgtaatattt taacgcccga taatattctt attgggcgtt
1561 ttcagtttct tcatcgcgac gacgaataat caaccccgcc agccagaaac taatcaccca
1621 cgtcaccaga ccgacggcat aggtctgcca accttttgct tcaaatccag taacccacca
1681 cgccatt
//