GenBank-Updates@genbank.bio.net (03/14/91)
LOCUS LACLACRABC 9323 bp ds-DNA BCT 14-MAR-1991
DEFINITION Lactococcus lactis repressor (lacR), galactose 6-P isomerase LacA
and LacB subunit, tagatose 6-P kinase (lacC), tagatose 1,6-diP
aldolase (lacD), enzyme III (lacF), enzyme II (lacE), and
phospho-beta-galactosidase (lacG) genes, complete cds.
ACCESSION M60447
KEYWORDS LacE enzyme II; LacF enzyme III;
galactose 6-P isomerase LacA subunit;
galactose 6-P isomerase LacB subunit; phospho-beta-galactosidase;
repressor lacR; tagatose 1,6-diP aldolase; tagatose 6-P kinase;
transposase.
SOURCE Loactococcus lactis lactis (strain MG 1820) DNA.
ORGANISM Lactococcus lactis
Prokaryota; Bacteria; Firmicutes; Regular asporogenous rods;
Lactobacillaceae.
REFERENCE 1 (bases 1 to 9323)
AUTHORS de Vos,W.M., Boerrigter,I., van Rooyen,R.J., Reiche,B. and
Hengstenberg,W.
TITLE Characterization of the lactose-specific enzymes of the
phosphotransferase system in Lactococcus lactis
JOURNAL J. Biol. Chem. 265, 22554-22560 (1990)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS complement(178..963)
/product="repressor protein"
/gene="lacR"
/codon_start=963
CDS 1433..1858
/product="galactose 6-P isomerase LacA subunit"
/gene="lacA"
/codon_start=1433
CDS 1875..2390
/product="galactose 6-P isomerase LacB subunit"
/gene="lacB"
/codon_start=1875
CDS 2401..3333
/product="tagatose 6-P kinase"
/gene="lacC"
/codon_start=2401
CDS 3338..4318
/product="tagatose 1,6-diP aldolase"
/gene="lacD"
/codon_start=3338
CDS 4344..4661
/product="enzyme III"
/gene="lacF"
/codon_start=4344
CDS 4665..6371
/product="enzyme III"
/gene="lacE"
/codon_start=4665
CDS 6476..7882
/product="phospho-beta-galactosidase"
/gene="lacG"
/codon_start=6476
CDS 8174..9073
/note="ORF"
/gene="lacX"
/codon_start=8174
CDS complement(9135..9322)
/product="transposase"
/gene="iso-ISS1"
/codon_start=9320
/partial
BASE COUNT 2927 a 1595 c 1881 g 2920 t
ORIGIN
1 gatatccaat tatcaagaaa actccaattt tttggaaagt taaataatgt gtacatattt
61 tcgacaaacc atacattaga aataacaaaa aaacaccgta gaattgtttg attcatacgg
121 tgcttttttt atgtctaatt atttaagagt agtccaatat ttttataagt cataattcta
181 gtccgctttt aaaatagtag tgtactggct aaattcctct aagtgttgag ggctaatctg
241 gttatctgta atgatggtat cgagttgatc tagattgtag aagttaaaga aatcgtatcg
301 atcgaattta gtactgtcta ctaataagaa tttttctaca gcattgttta gggcaagttg
361 ttgaatcaca ccttccttgt cactatatgt agcaatagaa ttatgggtaa cagcatttgc
421 acgaacaaaa gctttggcaa atctcattgc ttttaaattt gtcgaagcca ttgaacctac
481 aaaagctcca gttatttctc tatattcacc gccaagaagc aataaatcaa tggtttcgct
541 atcatttaga atcaagaaca cagggagact atttgtaatg acacgaattt tataaccttt
601 acgacctttc aattctaatg ccagttgtac aagtgtagtt ccgggtccaa taaaaatagt
661 atcgccatct ttgattaaag agcaggcttt tttagcaatg tcaatttttt cttttgtatt
721 tagacttttc ttctcgatat gtgtcttttc aagtggcttt ttagaggaaa gcaattgtgc
781 accaccatga gtacgtgtta aaagtccatc agcttctaga gcatcaaggt ctctacgggc
841 tgtcatatcg gaaatatcta gttcatctat tatttctttt atggttatgg tcccatcaat
901 ctttaacata tctaaaattt tttctaatcg tcgttttttg ttcatatgaa gactttcttt
961 cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa agataatatt
1021 ttcaaggtaa aacaaacaat ttcaaacaaa aacaaacgtt agatgatgaa ataagaacag
1081 aggattgacg tatattagct taggtcagat tttgtataag acgaaaataa agtaggacct
1141 cttaatcagt aagttataga aagtaaaaga cttttgtaat acctgaatag atatttcacg
1201 tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt gttatctatt
1261 ttttaaaaaa acaaataaaa aaaaacaaaa aattaacaaa aatagttgcg ttttgtttga
1321 atgtttgata tcatataaac aaagaaatga tgaaaacgtt atcttgaaca ttttgcaaaa
1381 tattttctac ttctacgtag catttctttt taaaatttag gaggtagtcc aaatggctat
1441 tgttgttggt gcagatctca aaggtacgag attaaaagat gttgtgaaaa attttcttgt
1501 agaagaaggt tttgaggtaa ttgatgtgac taaggacgga caagattttg ttgatgttac
1561 cttggcagtt gcctctgaag taaataaaga tgagcaaaac ttaggtattg taattgatgc
1621 ttatggagct ggtccattca tggtagcaac taaaattaaa ggtatggttg cagcagaagt
1681 ttcagatgaa cgctcagctt atatgacacg tggacataac aatgcacgaa tgattacggt
1741 tggcgctgaa atcgtcggtg atgagcttgc taaaaacatc gctaaagctt tcgtcaatgg
1801 taaatatgat ggcggacgtc atcaagtccg agtagatatg cttaacaaga tgtgttaaga
1861 aaaggagtta aaaaatgaga atcgcaattg gatgtgacca cattgtcaca gatgtaaaaa
1921 tggcagtatc agaattcttg aaatcaaaag gatatgaggt ccttgatttc gggacatatg
1981 accacgtacg tactcactac cctatctatg gtaaaaaagt cggtgaagca gtagtaagtg
2041 gtcaagcaga cttgggagta tgtatctgtg gtacgggtgt cggcattaac aatgcagtca
2101 ataaagttcc aggtgttcgc tcagcgttgg ttcgtgatat gacgtcagct ctatatgcta
2161 aggaagaact taatgcgaat gttatcggtt ttggtggaat gattactggt ggtcttctta
2221 tgaatgacat cattgaagct ttcattgaag ctgagtacaa accaacagaa gaaaataaaa
2281 aattgattgc aaaaatcgaa cacgttgaaa cacataatgc acatcaagca gatgaggaat
2341 tctttacaga attccttgaa aaatgggacc gtggtgagta ccacgattaa ggtataacca
2401 atgattctga cagtcacact aaatccttca gtagacatct cttatcctct agaaacattg
2461 aaaattgata cagtgaatag ggtgaaagat gttagtaaga cagctggtgg aaaaggctta
2521 aatgttacta gagtcttgta tgaatctggt gataaagtta ctgctacagg tttcttaggt
2581 ggtaaaattg gtgaatttat tgaaagcgaa ttagaacaat ctcctgttag tccagctttt
2641 tataaaatct caggaaacac tagaaactgt attgctattt tacacgaggg taaccaaaca
2701 gaaattttgg aacaaggacc aacaatttct catgaagaag cagaaggctt tcttgaccac
2761 tatagcaatc ttattaagca atcagaagtt gtcacaattt caggaagttt gccttcagga
2821 cttcccaacg attattatga aaaacttatc caacttgctt cggatgaagg agtagcagtc
2881 gttttggatt gttcaggtgc accacttgaa acagttctaa aatcgtcggc taaacctact
2941 gccatcaaac ctaataatga ggaactttca cagttgcttg gaaaagaagt aacaaaagat
3001 attgaagaac tcaaagacgt tcttaaagag tcacttttta gtggtatcga atggatagtg
3061 gtctcattag gtcgaaacgg tgcctttgca aaacatggtg atgttttcta taaggtagat
3121 attcctgata tcccagttgt taatccagtc ggatcagggg actcaacggt agctggtatt
3181 gcatcagctt taaatagtaa aaaaagtgac gctgacttat taaaacatgc gatgacattg
3241 ggtatgttaa atgctcaaga aacaatgaca gggcatgtga atatgactaa ttacgaaaca
3301 ctaaactcac aaattggagt aaaagaggta taaaaatggt acttacagaa cagaaacgca
3361 aatctttgga aaaactttca gataaaaacg gttttatctc agctttggca tttgaccaac
3421 gtggtgcttt gaaacgtttg atggcacagt atcaagatac agaaccaact gtagctcaaa
3481 tggaagaact aaaggttttg gttgctgatg aacttacaaa gtacgcttct tcaatgttgc
3541 ttgaccctga atatggactt ccagcaacaa aagcattgga taaagaagca ggtcttcttc
3601 ttgcctttga aaaaacaggt tatgatacat caagtacaaa acgtttgcct gactgcttgg
3661 atgtttggtc tgcaaagcgt atcaaagagc aaggcgctga tgccgttaag ttcttgcttt
3721 actatgatgt agacagctca gatgaactta accaacaaaa acaagcttat attgaacgtg
3781 ttggttctga atgtgtcgct gaagatattc ctttcttctt ggaaatcctt gcttatgatg
3841 aagaaatctc agatgcaggt tccgttgaat atgcaaaagt gaaacctcgc aaagtcattg
3901 aagcgatgaa agttttctct gatccacgct ttaacattga tgttcttaaa gtagaagtac
3961 cagttaacgt taaatatgtc gaaggttttg ctgatggtga agtggtttac agtaaagctg
4021 aagctgctga cttctttaaa gcacaagaag aagcgactaa ccttccatac atctacctaa
4081 gtgcaggtgt atctgctaaa ttgttccaag aaacattgca atttgctcac gactcaggtg
4141 ccaagtttaa tggtgtgctt tgtggacgtg ctacttgggc aggatctgta gaaccttaca
4201 tcaaagaagg tgaaaaagca gcacgtgaat ggttgcgtac tactggattc gaaaatattg
4261 atgaacttaa caaagttctt gttaaaacag ctagtccatg gactgataaa gtatagtaaa
4321 aacataaaac ggaggatatt gttgtgaaca gagaagagat gactctctta gggtttgaaa
4381 ttgttgctta tgctggagat gctcgctcta agcttttaga agcgcttaaa gcggctgaaa
4441 atggtgattt cgctaaggca gatagtcttg tagtagaagc aggaagctgt attgcagagg
4501 ctcacagttc tcagacaggt atgttggctc gagaagcttc tggggaggaa cttccataca
4561 gtgttactat gatgcatggt caggatcact tgatgactac gatcttatta aaagatgtga
4621 ttcatcacct catcgaactt tataaaagag gagcaaagta attaatgcat aaactcattg
4681 aacttattga gaaagggaaa ccattctttg agaaaatctc tcgaaacatc tatcttcgtg
4741 ctattcgtga tggatttatt gctggtatgc cagtcatcct tttctcatct atctttatcc
4801 ttattgccta tgtcccaaat gcttggggat tccactggtc aaaagacatt gagaccttct
4861 tgatgactcc ttatagctat tcgatgggta tcctagcatt ctttgttggt ggtactaccg
4921 ctaaagcttt gacggactca aagaaccgtg acctacctgc gaccaatcag attaacttct
4981 tatctacgat gctagcgtct atggtagggt tccttttgat ggcagctgag cctgcaaaag
5041 aaggaggctt cttgacagcc ttcatgggaa caaaaggcct tttgacagca tttatcgcag
5101 cctttgtgac agttaatgtt tataaagtct gtgtaaaaaa taatgttacc attcgtatgc
5161 ctgaagatgt tccaccaaat atctctcaag tatttaagga cttgattccg ttcaccgtat
5221 cagttgttct cctatatgga cttgaactcc ttgttaaggg aactcttggt gtaactgttg
5281 cagaatcaat cggtaccctt attgctcctc ttttctcagc tgcagatggt taccttggaa
5341 ttacacttat ctttggtgct tatgccttct tctggtttgt tggtattcac ggtccttcaa
5401 ttgtcgaacc agcaatcgct gctatcactt atgccaatat cgatgtcaac ttgcatctta
5461 tccaagctgg acaacatgca gataaagtta tcacttctgg tactcaaatg tttattgcta
5521 ccatgggtgg aacaggagct acattgattg ttccattctt gttcatgtgg atttgtaaat
5581 cagatcgtaa ccgtgccatc ggacgtgcct cagttgttcc aacattcttt ggggttaatg
5641 agccaatctt atttggtgca ccaatcgtat tgaatccgat tttctttgta ccgttcattt
5701 tcgctccgat tgtcaacgtt tggatcttta aattctttgt tgacacattg aacatgaact
5761 cattctctgc caaccttcca tgggttactc ctggtccgtt gggaattgta ctcggtacta
5821 acttccaagt tctatcattt atcttagccg gactcttggt agttgttgat actatcattt
5881 attacccatt tgttaaggta tacgatgaac aaattcttga agaagaacgt tctggtaaaa
5941 caaacgatgc tcttaaagaa aaagtagctg caaacttcaa tactgctaaa gcagatgccg
6001 ttcttggaaa agcagacgtt gctaaagaag atgtagcagc aaacaataat atcacaaaag
6061 aaactaatgt ccttgttctt tgtgcaggtg gaggtacaag tggcttgctt gccaatgcct
6121 tgaacaaagc agcagcagaa tacaatgttc cagtcaaggc ggcagcgggt ggttacggtg
6181 ctcaccgtga aatgttgcca gagtttgact tggtaatctt ggctccacag gtcgcttcaa
6241 acttcgatga tatgaaggct gaaacagata aattgggcat taaacttgta aaaactgaag
6301 gagctcaata tatcaaattg acacgtgacg gacaaggtgc tcttgcattt gttcaacaac
6361 agtttgatta ataaatttgt aaaagttatt tgagacgaga tcactcattc catttccttg
6421 gttttcagaa cacagaatct cgtctcaaat gctttttttg aaaggactta cacttatgac
6481 taaaacactt cctaaagatt ttatttttgg tggtgctaca gctgcttatc aagcagaagg
6541 tgcaactcac acagatggta agggcccagt agcatgggat aagtatcttg aagacaatta
6601 ttggtataca gcagaacctg caagtgattt ttaccataaa tacccagttg atttagaatt
6661 agctgaagaa tatggtgtta atggtattcg tatttctatt gcctggtctc gtattttccc
6721 aacgggttac ggtgaagtaa atgaaaaagg tgttgaattc taccataaac tatttgctga
6781 atgccacaag cgtcatgttg aaccatttgt gactttgcat cattttgaca caccagaagc
6841 tcttcattca aatggagatt tcttaaaccg cgaaaatata gaacacttta tagattatgc
6901 cgctttctgt tttgaagagt ttccagaagt aaactactgg acaactttca atgaaattgg
6961 cccaattggt gatggtcaat acttggttgg taaattccct ccaggcatta aatatgatct
7021 tgccaaagtc ttccaatcac accacaacat gatggtctct catgcacgtg ctgtaaaatt
7081 gtataaggat aaaggttata aaggtgaaat tggtgttgtt catgctttac caactaaata
7141 cccttatgat ccggaaaatc cagcagatgt tcgtgctgct gaacttgaag acatcatcca
7201 taacaaattt atcttggatg caacctatct tgggcactat tcagataaaa cgatggaagg
7261 tgtcaaccat atcctagctg agaatggtgg agaacttgat cttcgtgatg aagacttcca
7321 agctcttgac gcagctaaag atttgaatga tttccttggt atcaactatt acatgagtga
7381 ttggatgcaa gcttttgatg gtgagactga aatcattcac aatggtaagg gtgaaaaagg
7441 aagctctaag taccaaatta agggtgttgg tcgtcgagta gctcctgact atgttccgcg
7501 cacagactgg gattggatta tttatcctga aggcttgtat gaccaaatca tgcgagtgaa
7561 aaatgattat ccgaattaca agaagattta catcactgaa aacggtctcg gatacaaaga
7621 tgagtttgta gataatactg tttatgatga tggtcgtatt gattacgtga agcaacactt
7681 ggaagtttta tcagacgcga ttgcggatgg tgcaaatgtt aaaggttact tcatttggtc
7741 acttatggac gttttctcat ggtcaaatgg ttatgaaaaa cgttatggat tgttctatgt
7801 agactttgat acgcaagaac gctatcctaa gaaatcagca cattggtata agaaattagc
7861 agaaactcaa gtgatagagt aatatcacct gagaatgtaa taatttgtta tctgattttt
7921 agatcaggct ttgaaaaggt atatattatt aatttcgaag attgtcctat aattcctgag
7981 cttgaagcta cctcactaca aaatgtagta actggtatgt ttcacctgga aaagaaagga
8041 tttgccattc tgatttagca ttatccgaat ttgaatggga agttttacaa ttactcgatg
8101 tagcaatatg tgagactgtt cacttcgagg tagattattt gcctttccaa taacaaaaag
8161 aaaggaattg actgtgacta tcgaattaaa aaacgagtat cttacggttc agtttaaaac
8221 acttggtggt caactgactt cgattaaaga caaagatggg ttagaatatc tctggcaagc
8281 tgatccagag tattggaatg gacaggcacc aattctattt cctatctgtg gtagtttacg
8341 taatgattgg gctatttata gacctcaaga aagacctttt tttacaggtc taattcgtag
8401 acatggtttt gttcgaaaag aagaatttac tcttgaagaa gttaatgaaa atagcgtgac
8461 tttcagtatt aaaccaaatg ctgagatgct tgataattac ctttatcagt ttgaactaag
8521 agttgtttat accttaaatg ggaaatccat tagaaccgag tttcaggtaa cgaacttgga
8581 aactgaaaaa acgatgcctt acttcattgg agcccatcca gcattcaatt gtcctttagt
8641 agaaggcgaa aagtatgagg actattctct tgaatttagt gaggttgagt cttgttcgat
8701 accaaagagt ttccctgaga cagggttatt agatttacaa gatcggacgc catttttgga
8761 aaaccaaaag tctcttgatt tggattactc acttttttct catgacgcca ttacacttga
8821 ccgactgaaa tctcgcagcg tgacacttcg ctcgagaaag tctggtaaag gtcttcgagt
8881 tgattttgat gattttccaa acttgattct gtggtctaca accaataaaa gtccattcat
8941 tgccttagaa ccttggagtg ggctttccac ttcacttgaa gaaggtaata ttttagaaga
9001 taaaccacag gtaacgaaag ttttacctct agatacttct aaaaagtcct atgacattac
9061 gatccttaat taaaatctgt ggttctgttg cgaagtttga aaatcaaacg cattctctct
9121 gaactccaaa tatcttaggc tgttattccc attaatacct tgatttcagt agacaccgaa
9181 aagccgaaga gcgttccatt tcttcggttc tttttatata ttcctcgaag ggtctttatg
9241 cccttaatcg tggaagaggc tgtacggaga ctttgacaaa atttattccg tcgtttaata
9301 ggttgatggt cttgttctat taa
//