GenBank-Updates@genbank.bio.net (02/07/91)
LOCUS ECOBGLO 5270 bp ds-DNA BCT 07-FEB-1991
DEFINITION E.coli bgl operon encoding a positive regulatory protein, a
transport protein, and phospho-beta-glucosidase, complete cds, and
phoU gene, 3' end.
ACCESSION M16487
KEYWORDS bglB gene; bglC gene; bglS gene; insertion element IS5; phoU gene;
phospho-beta-glucosidase; positive regulatory protein;
transport protein.
SOURCE E.coli K12 DNA, clone pFDX733.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 5270)
AUTHORS Schnetz,K., Toloczyki,C. and Rak,B.
TITLE Beta-glucoside (bgl) operon of Escherichia coli K-12: Nucleotide
sequence, genetic organization, and possible evolutionary
relationship to regulatory components of two Bacillus subtilis
genes
JOURNAL J. Bacteriol. 169, 2579-2590 (1987)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [J. Bacteriol. 169,
2579-2590 (1987)] kindly provided
by B.Rak, 04-MAR-1988.
FEATURES Location/Qualifiers
CDS <1..296
/note="phoU protein /nomgen='phoU'"
misc_feature 305..337
/note="region of dyad symmetry (possible termination
signal)"
misc_recomb 374^375
/note="site of IS5 insertion"
misc_feature 515..556
/note="region of dyad symmetry"
CDS 582..1418
/note="positive regulatory protein /nomgen='bglC'"
misc_feature 1462..1513
/note="region of dyad symmetry"
CDS 1552..3429
/note="transport protein /nomgen='bglS'"
CDS 3448..4863
/note="phospho-beta-glucosidase /nomgen='bglB'"
misc_feature 4860..4903
/note="region of dyad symmetry"
BASE COUNT 1392 a 1125 c 1348 g 1405 t
ORIGIN 1 bp upstream of MluI site.
1 acgcgttcgc gcggatggac attgacgaag cggtacgtat ttatcgtgaa gataaaaaag
61 tcgatcagga atacgaaggt attgttcgtc aactgatgac ctacatgatg gaagattcgc
121 gtaccattcc gagcgtactt actgcgctgt tctgcgcgcg ttctatcgaa cgtattggcg
181 accgctgcca gaatatttgt gagtttatct tctactacgt gaaggggcag gatttccgtc
241 acgtcggtgg cgatgagctg gataaactgc tggcggggaa agatagcgac aaataattca
301 ccagacaaat cccaataact taattattgg gatttgttat atataacttt ataaattcct
361 aaaattacac aaagttaata actgcgagca tggtcatatt tttatcaata gcgcattgct
421 attttctctg cacgcaatta aattaatttc cgaacctgga tgttcgttat aaaaaccatt
481 aataaatgac tggattgtta ctgcattcgc aggcaaaacc tgacataacc agagaatact
541 ggtgaagtcg ggtttttttg tttataaaaa aggtccttgc tatgaacatg caaatcacca
601 aaattctcaa caataatgtt gtggtggtta ttgatgatca acagcgggaa aaagtcgtca
661 tggggcgcgg aattggcttt caaaaacgcg ctggcgaaag aattaactca agtggaatag
721 aaaaagagta tgccttgagc agtcatgaac tgaacgggcg attaagcgaa ctcttaagtc
781 atattcctct tgaggtgatg gcaacctgtg atcgtattat ctctttagcg caggagcgct
841 tgggaaaatt acaggacagt atttatatct cgctaactga ccattgccag tttgcgatta
901 aacgctttca gcaaaacgtg ttgctgccca acccgttgct gtgggatatc cagcggcttt
961 acccgaaaga gttccagcta ggggaagaag cgttaaccat tattgataaa cggttgggcg
1021 tgcagttacc gaaagatgaa gtgggcttta ttgccatgca tctggtcagt gcccaaatga
1081 gcggaaatat ggaggatgtt gcaggtgtca cgcagttaat gcgcgaaatg ctgcaattaa
1141 taaaatttca gttcagcctt aattaccagg aagaaagctt gagttatcag cgactggtta
1201 cacatctgaa gtttttatcc tggcgtattc ttgaacatgc ttcaattaac gatagtgatg
1261 aatcattaca acaagcagta aaacaaaatt acccgcaagc atggcaatgt gcggaacgga
1321 tcgccatttt tattggtttg cagtatcaac gtaaaatttc acccgcagag attatgtttt
1381 tagccataaa tatagagcgc gtgcgcaaag aacactgaaa tattattact gagtaaagga
1441 ttgttaccgc actaagcggg caaaacctga aaaaaattgc ttgattcacg tcaggccgtt
1501 tttttcaggt ttttttttgg agttttgccg caaagcggta gagggcaagt tatgacggag
1561 ttagccagaa aaatagtcgc aggagtcggg ggcgcagata acattgtgag tctgatgcat
1621 tgcgcaacgc gattacgttt taaattaaag gatgaaagca aagcgcaagc agaggtactg
1681 aaaaagaccc ccggtattat tatggtggtg gaaagcggtg gccagtttca ggtggtcata
1741 ggtaaccatg tggccgatgt cttcctggcg gttaacagtg tggcaggcct tgacgaaaaa
1801 gcgcaacagg caccggaaaa tgatgataaa ggtaatctgc taaaccgctt tgtttatgtt
1861 atttcaggta tttttacgcc tctgatcggt ttgatggcgg caaccgggat cttgaaaggt
1921 atgctggctc tggcgctcac ttttcagtgg acgaccgaac aaagtggtac ttatttaatt
1981 ttattcagcg ccagtgatgc cttgttttgg ttcttcccga taatcctggg atacaccgcg
2041 gggaaacgct tcggcggtaa tccatttact gccatggtga ttggtggagc gttagtgcat
2101 ccattaattc tgactgcttt cgagaacggg caaaaagcgg atgcgctggg gctggatttc
2161 ctgggtattc cggtcacatt gttgaattac tcgtcatcgg ttattcccat tattttttct
2221 gcctggttgt gcagcattct ggaacgccga cttaatgcgt ggttaccgtc ggcaatcaaa
2281 aatttcttca caccattgct atgtctgatg gttatcacac ccgtcacctt tctgctggtg
2341 gggccgctat caacctggat aagcgaactg attgccgccg gttatctctg gctttatcag
2401 gcggttcctg catttgcggg cgcggtaatg ggcggcttct ggcaaatctt cgtcatgttc
2461 ggactgcact ggggcctggt gccgctgtgt atcaataact tcaccgtgct gggctacgac
2521 accatgatcc cgctgttaat gcccgccatt atggcgcagg tcggggcggc gctcggcgtc
2581 ttcctctgcg aacgcgatgc gcagaaaaaa gtggtggcgg gatcagcggc gttgacgagt
2641 ctgtttggta tcaccgaacc agcggtatat ggcgtcaacc tgccgcgtaa gtaccccttt
2701 gttatcgcct gtatcagtgg ggctttgggg gccaccatta ttggctacgc gcaaacgaaa
2761 gtctactcct ttggtttgcc aagtattttc accttcatgc aaaccatccc gtcaacggga
2821 attgatttca ccgtctgggc cagcgttatt ggcggtgtca ttgccatcgg ttgcgcattt
2881 gtcggtacgg tgatgcttca tttcatcacc gctaaacgtc agccagcgca gggtgccccg
2941 caagagaaaa caccagaggt tattacacca cctgagcagg gcggtatctg ttcaccgatg
3001 acgggagaga ttgtgccgct cattcacgtc gctgatacca cgtttgccag tggcctgttg
3061 ggtaaaggta ttgccattct gccctcggtt ggtgaagtgc gttctccggt tgcgggtcga
3121 attgcttcgt tgttcgccac attacacgcc attggcattg agtcagatga tggtgtggag
3181 atcctgattc atgtcggtat cgacaccgta aaactggacg gcaaattctt ttccgctcac
3241 gtcaacgtgg gtgacaaggt caatacaggc gatcggctga tttcttttga tatccctgct
3301 attcgcgagg ccggatttga tctgacgacg ccggtattaa tcagtaatag cgatgatttt
3361 acggacgtat taccccacgg cacggcgcag ataagcgcag gtgaaccgct gttatccatc
3421 attcgctaac gataaaagga gttaattatg aaagcatttc cagaaacatt tctttggggt
3481 ggcgcaacag ctgccaatca ggtggaaggt gcctggcagg aagatggcaa agggatctcg
3541 acctcagatt tacagcctca tggcgtaatg ggaaaaatgg aaccgcgcat cctggggaaa
3601 gagaatatca aagatgtcgc catcgatttt tatcaccgtt acccggaaga tatcgcgtta
3661 tttgccgaga tgggcttcac ctgtctgcgt atttccattg cctgggcgcg aattttccct
3721 cagggcgacg aagtcgaacc gaatgaagcg gggttagcgt tttacgatcg gctgtttgat
3781 gaaatggcgc aggcggggat caagccgctg gtaacgttat cccattacga aatgccatat
3841 gggctggtga aaaactacgg cggttgggct aatcgagcgg tcatcggtca cttcgagcat
3901 tacgcccgca cggtctttac tcgctaccaa cataaagtgg cgttatggct gacgtttaat
3961 gaaatcaaca tgtcgttaca cgcgccattc acgggcgtgg ggctggcaga agagagtggc
4021 gaggcggaag tttatcaggc tatccaccat caactggttg ccagtgcgcg ggcagttaaa
4081 gcctgtcata gcctgctccc cgaagcgaaa atcggcaata tgcttctcgg tgggctcgtt
4141 taccccctca cctgccagcc acaggatatg ttgcaggcca tggaagagaa ccggcgctgg
4201 atgttctttg gtgatgttca ggcgcgtggc cagtatcccg gctatatgca gcgtttcttc
4261 cgcgaccaca atatcaccat tgagatgact gaaagtgacg cagaagattt aaaacatacc
4321 gtcgatttca tctcttttag ttattacatg actggttgtg tttcccacga cgaaagcatt
4381 aataaaaatg cgcagggcaa catactgaat atgatcccca atccgcatct gaaaagttca
4441 gagtgggggt ggcaaattga tccggttgga ttacgggttc tgttaaatac gctttgggat
4501 cgttatcaaa aaccgttatt tattgtcgag aacggattag gcgcaaaaga cagcgttgaa
4561 gcggatggtt cgatacagga cgattatcga attgcctatt taaacgatca cctggtacag
4621 gtaaatgaag cgattgccga tggtgtggat attatggggt acaccagttg ggggccaatt
4681 gatttagtca gtgcatctca ttcacaaatg tctaagcgct acggctttat ttatgtggat
4741 cgtgatgata atggcgaagg aagcctcaca agaacacgca agaaaagctt tcggatggta
4801 tgcgcagagg tgatcaagac gcgggggctg tcattaaaaa aaataaccat taaagcacct
4861 taattatcgt cgcattcaga acagtctgga tgcgatgcgt taattctttc tttgcaccat
4921 aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta atggcaccgt
4981 tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc cttgagttat
5041 tagaaaaggc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat gaagagaaga
5101 aaaagtatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa gggtatgccg
5161 ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc aaaaatgaag
5221 agaaaaatgc cagtgagaca ggctcgattt attcttccat gactctgaaa
//GenBank-Updates@genbank.bio.net (03/15/91)
LOCUS ECOBGLO 5270 bp ds-DNA BCT 15-MAR-1991
DEFINITION E.coli bgl operon encoding a positive regulatory protein, a
transport protein, and phospho-beta-glucosidase, complete cds, and
phoU gene, 3' end.
ACCESSION M16487 M60288
KEYWORDS bglB gene; bglC gene; bglS gene; insertion element IS5; phoU gene;
phospho-beta-glucosidase; positive regulatory protein;
transport protein.
SOURCE E.coli K12 DNA, clone pFDX733.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 5270)
AUTHORS Schnetz,K., Toloczyki,C. and Rak,B.
TITLE beta-glucoside (bgl) operon of Escherichia coli K-12: Nucleotide
sequence, genetic organization, and possible evolutionary
relationship to regulatory components of two Bacillus subtilis
genes
JOURNAL J. Bacteriol. 169, 2579-2590 (1987)
STANDARD full staff_entry
REFERENCE 2 (sites)
AUTHORS Houman,F., Dias-Torres,M. and Wright,A.
TITLE Transcriptional antitermination in the bgi operon of E. coli is
modulated by a specific RNA binding protein
JOURNAL Cell 62, 1153-1163 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [J. Bacteriol. 169,
2579-2590 (1987)] kindly provided
by B.Rak, 04-MAR-1988.
FEATURES Location/Qualifiers
CDS <1..296
/note="phoU protein /nomgen='phoU'"
/codon_start=1
misc_feature 305..337
/note="region of dyad symmetry (possible termination
signal)"
misc_recomb 374^375
/note="site of IS5 insertion"
mRNA 452..1418
/product="positive regulatory protein"
/gene="bglC"
/partial
CDS 486..545
/note="ORF 19"
/codon_start=486
misc_feature 515..556
/note="region of dyad symmetry"
terminator 515..556
CDS 582..1418
/note="positive regulatory protein /nomgen='bglC'"
/product="positive regulatory protein"
/gene="bglC"
/codon_start=582
misc_feature 1462..1513
/note="region of dyad symmetry"
CDS 1552..3429
/note="transport protein /nomgen='bglS'"
/codon_start=1552
CDS 3448..4863
/note="phospho-beta-glucosidase /nomgen='bglB'"
/codon_start=3448
misc_feature 4860..4903
/note="region of dyad symmetry"
BASE COUNT 1392 a 1125 c 1348 g 1405 t
ORIGIN 1 bp upstream of MluI site.
1 acgcgttcgc gcggatggac attgacgaag cggtacgtat ttatcgtgaa gataaaaaag
61 tcgatcagga atacgaaggt attgttcgtc aactgatgac ctacatgatg gaagattcgc
121 gtaccattcc gagcgtactt actgcgctgt tctgcgcgcg ttctatcgaa cgtattggcg
181 accgctgcca gaatatttgt gagtttatct tctactacgt gaaggggcag gatttccgtc
241 acgtcggtgg cgatgagctg gataaactgc tggcggggaa agatagcgac aaataattca
301 ccagacaaat cccaataact taattattgg gatttgttat atataacttt ataaattcct
361 aaaattacac aaagttaata actgcgagca tggtcatatt tttatcaata gcgcattgct
421 attttctctg cacgcaatta aattaatttc cgaacctgga tgttcgttat aaaaaccatt
481 aataaatgac tggattgtta ctgcattcgc aggcaaaacc tgacataacc agagaatact
541 ggtgaagtcg ggtttttttg tttataaaaa aggtccttgc tatgaacatg caaatcacca
601 aaattctcaa caataatgtt gtggtggtta ttgatgatca acagcgggaa aaagtcgtca
661 tggggcgcgg aattggcttt caaaaacgcg ctggcgaaag aattaactca agtggaatag
721 aaaaagagta tgccttgagc agtcatgaac tgaacgggcg attaagcgaa ctcttaagtc
781 atattcctct tgaggtgatg gcaacctgtg atcgtattat ctctttagcg caggagcgct
841 tgggaaaatt acaggacagt atttatatct cgctaactga ccattgccag tttgcgatta
901 aacgctttca gcaaaacgtg ttgctgccca acccgttgct gtgggatatc cagcggcttt
961 acccgaaaga gttccagcta ggggaagaag cgttaaccat tattgataaa cggttgggcg
1021 tgcagttacc gaaagatgaa gtgggcttta ttgccatgca tctggtcagt gcccaaatga
1081 gcggaaatat ggaggatgtt gcaggtgtca cgcagttaat gcgcgaaatg ctgcaattaa
1141 taaaatttca gttcagcctt aattaccagg aagaaagctt gagttatcag cgactggtta
1201 cacatctgaa gtttttatcc tggcgtattc ttgaacatgc ttcaattaac gatagtgatg
1261 aatcattaca acaagcagta aaacaaaatt acccgcaagc atggcaatgt gcggaacgga
1321 tcgccatttt tattggtttg cagtatcaac gtaaaatttc acccgcagag attatgtttt
1381 tagccataaa tatagagcgc gtgcgcaaag aacactgaaa tattattact gagtaaagga
1441 ttgttaccgc actaagcggg caaaacctga aaaaaattgc ttgattcacg tcaggccgtt
1501 tttttcaggt ttttttttgg agttttgccg caaagcggta gagggcaagt tatgacggag
1561 ttagccagaa aaatagtcgc aggagtcggg ggcgcagata acattgtgag tctgatgcat
1621 tgcgcaacgc gattacgttt taaattaaag gatgaaagca aagcgcaagc agaggtactg
1681 aaaaagaccc ccggtattat tatggtggtg gaaagcggtg gccagtttca ggtggtcata
1741 ggtaaccatg tggccgatgt cttcctggcg gttaacagtg tggcaggcct tgacgaaaaa
1801 gcgcaacagg caccggaaaa tgatgataaa ggtaatctgc taaaccgctt tgtttatgtt
1861 atttcaggta tttttacgcc tctgatcggt ttgatggcgg caaccgggat cttgaaaggt
1921 atgctggctc tggcgctcac ttttcagtgg acgaccgaac aaagtggtac ttatttaatt
1981 ttattcagcg ccagtgatgc cttgttttgg ttcttcccga taatcctggg atacaccgcg
2041 gggaaacgct tcggcggtaa tccatttact gccatggtga ttggtggagc gttagtgcat
2101 ccattaattc tgactgcttt cgagaacggg caaaaagcgg atgcgctggg gctggatttc
2161 ctgggtattc cggtcacatt gttgaattac tcgtcatcgg ttattcccat tattttttct
2221 gcctggttgt gcagcattct ggaacgccga cttaatgcgt ggttaccgtc ggcaatcaaa
2281 aatttcttca caccattgct atgtctgatg gttatcacac ccgtcacctt tctgctggtg
2341 gggccgctat caacctggat aagcgaactg attgccgccg gttatctctg gctttatcag
2401 gcggttcctg catttgcggg cgcggtaatg ggcggcttct ggcaaatctt cgtcatgttc
2461 ggactgcact ggggcctggt gccgctgtgt atcaataact tcaccgtgct gggctacgac
2521 accatgatcc cgctgttaat gcccgccatt atggcgcagg tcggggcggc gctcggcgtc
2581 ttcctctgcg aacgcgatgc gcagaaaaaa gtggtggcgg gatcagcggc gttgacgagt
2641 ctgtttggta tcaccgaacc agcggtatat ggcgtcaacc tgccgcgtaa gtaccccttt
2701 gttatcgcct gtatcagtgg ggctttgggg gccaccatta ttggctacgc gcaaacgaaa
2761 gtctactcct ttggtttgcc aagtattttc accttcatgc aaaccatccc gtcaacggga
2821 attgatttca ccgtctgggc cagcgttatt ggcggtgtca ttgccatcgg ttgcgcattt
2881 gtcggtacgg tgatgcttca tttcatcacc gctaaacgtc agccagcgca gggtgccccg
2941 caagagaaaa caccagaggt tattacacca cctgagcagg gcggtatctg ttcaccgatg
3001 acgggagaga ttgtgccgct cattcacgtc gctgatacca cgtttgccag tggcctgttg
3061 ggtaaaggta ttgccattct gccctcggtt ggtgaagtgc gttctccggt tgcgggtcga
3121 attgcttcgt tgttcgccac attacacgcc attggcattg agtcagatga tggtgtggag
3181 atcctgattc atgtcggtat cgacaccgta aaactggacg gcaaattctt ttccgctcac
3241 gtcaacgtgg gtgacaaggt caatacaggc gatcggctga tttcttttga tatccctgct
3301 attcgcgagg ccggatttga tctgacgacg ccggtattaa tcagtaatag cgatgatttt
3361 acggacgtat taccccacgg cacggcgcag ataagcgcag gtgaaccgct gttatccatc
3421 attcgctaac gataaaagga gttaattatg aaagcatttc cagaaacatt tctttggggt
3481 ggcgcaacag ctgccaatca ggtggaaggt gcctggcagg aagatggcaa agggatctcg
3541 acctcagatt tacagcctca tggcgtaatg ggaaaaatgg aaccgcgcat cctggggaaa
3601 gagaatatca aagatgtcgc catcgatttt tatcaccgtt acccggaaga tatcgcgtta
3661 tttgccgaga tgggcttcac ctgtctgcgt atttccattg cctgggcgcg aattttccct
3721 cagggcgacg aagtcgaacc gaatgaagcg gggttagcgt tttacgatcg gctgtttgat
3781 gaaatggcgc aggcggggat caagccgctg gtaacgttat cccattacga aatgccatat
3841 gggctggtga aaaactacgg cggttgggct aatcgagcgg tcatcggtca cttcgagcat
3901 tacgcccgca cggtctttac tcgctaccaa cataaagtgg cgttatggct gacgtttaat
3961 gaaatcaaca tgtcgttaca cgcgccattc acgggcgtgg ggctggcaga agagagtggc
4021 gaggcggaag tttatcaggc tatccaccat caactggttg ccagtgcgcg ggcagttaaa
4081 gcctgtcata gcctgctccc cgaagcgaaa atcggcaata tgcttctcgg tgggctcgtt
4141 taccccctca cctgccagcc acaggatatg ttgcaggcca tggaagagaa ccggcgctgg
4201 atgttctttg gtgatgttca ggcgcgtggc cagtatcccg gctatatgca gcgtttcttc
4261 cgcgaccaca atatcaccat tgagatgact gaaagtgacg cagaagattt aaaacatacc
4321 gtcgatttca tctcttttag ttattacatg actggttgtg tttcccacga cgaaagcatt
4381 aataaaaatg cgcagggcaa catactgaat atgatcccca atccgcatct gaaaagttca
4441 gagtgggggt ggcaaattga tccggttgga ttacgggttc tgttaaatac gctttgggat
4501 cgttatcaaa aaccgttatt tattgtcgag aacggattag gcgcaaaaga cagcgttgaa
4561 gcggatggtt cgatacagga cgattatcga attgcctatt taaacgatca cctggtacag
4621 gtaaatgaag cgattgccga tggtgtggat attatggggt acaccagttg ggggccaatt
4681 gatttagtca gtgcatctca ttcacaaatg tctaagcgct acggctttat ttatgtggat
4741 cgtgatgata atggcgaagg aagcctcaca agaacacgca agaaaagctt tcggatggta
4801 tgcgcagagg tgatcaagac gcgggggctg tcattaaaaa aaataaccat taaagcacct
4861 taattatcgt cgcattcaga acagtctgga tgcgatgcgt taattctttc tttgcaccat
4921 aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta atggcaccgt
4981 tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc cttgagttat
5041 tagaaaaggc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat gaagagaaga
5101 aaaagtatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa gggtatgccg
5161 ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc aaaaatgaag
5221 agaaaaatgc cagtgagaca ggctcgattt attcttccat gactctgaaa
//