GenBank-Updates@genbank.bio.net (03/06/91)
LOCUS RATMTACAL 1715 bp ss-mRNA ORG 06-MAR-1991
DEFINITION Rat mitochondrial acetoacetyl-CoA thiolase mRNA, complete cds.
ACCESSION D00512
KEYWORDS acetoacetyl-CoA thiolase.
SOURCE Rat liver free polysomes, cDNA to mRNA, clones RT2-7 and RT2-8.
ORGANISM Mitochondrion Rattus sp.
REFERENCE 1 (bases 1 to 1715)
AUTHORS Fukao,T., Kamijo,K., Osumi,T., Fujiki,Y., Yamaguchi,S., Orii,T. and
Hashimoto,T.
TITLE Molecular cloning and nucleotide sequence of cDNA encoding the
entire precursor of rat mitochondrial acetoacetyl-CoA thiolase
JOURNAL J. Biochem. 106, 197-204 (1989)
STANDARD full staff_entry
COMMENT The amino-terminal sequence of the mature enzyme was determined by
Edman degradation.
FEATURES Location/Qualifiers
CDS 21..1295
/note="mitochondrial acetoacetyl-CoA thiolase precursor"
mat_peptide 111..1292
/note="mitochondrial acetoacetyl-CoA thiolase mature
protein"
misc_signal 1444..1449
/note="polyadenylation signal"
misc_signal 1703..1708
/note="polyadenylation signal"
BASE COUNT 500 a 377 c 442 g 396 t
ORIGIN 20 bp upstream of the initiation codon.
1 gacaagcttt tccggtctcc atggctgccc tggcggttct acacggcgtc gtccgcaggc
61 ctctgctccg cgggctgctg caggaagtaa gatgcctggg acgaagttat gcatccaaac
121 ccactttgaa tgatgtggtt atagtgagtg ctacacgaac tcccattgga tccttcctgg
181 gcagccttgc ctctcagcca gccaccaagc ttggtactat tgcaattcag ggagccattg
241 aaaaggcagg gattccaaaa gaagaagtga aggaggtcta catgggcaac gtcatccaag
301 ggggagaagg acaggccccg accaggcaag ctacactggg tgcaggtcta cccattgcca
361 ctccgtgcac cacagtaaac aaggtgtgtg cctcaggaat gaaagccatc atgatggcct
421 ctcaaagtct tatgtgtgga caccaggatg tgatggtggc aggcggaatg gagaccatgt
481 caaatgtccc gtacgtaatg agcagaggag caacaccata cggtggggta aaacttgaag
541 acctgattgt gaaagacggg ctaaccgatg tctacaataa aattcatatg ggcaattgtg
601 ctgagaacac cgcgaagaag ctgagtatct cgcgggagga gcaggataag tacgccatcg
661 gctcttacac ccgaagtaaa gaggcgtggg atgcagggaa gtttgcaaat gagattacgc
721 ccatcaccat ctcagtgaaa ggtaaaccag acgtggtggt gaaggaagat gaagagtaca
781 agcgagttga cttcagtaaa gtgccaaagc tcaagacagt gttccagaaa gaaaacggca
841 cagtaacagc tgctaacgcc agcacactga acgacggagc agctgctgtg gttctcatga
901 ctgcagaggc agcccagcgg ctcaaggtta agccactggc acgaatcgca gcatttgctg
961 atgctgctgt agaccccatt gatttcccac tcgcacctgc atatgctgta cctaaggttc
1021 ttaaatatgc aggactgaaa aaagaagaca ttgccatgtg ggaagtaaat gaagcattca
1081 gtgtggttgt actagccaac attaaaatgc tggagattga ccctcaaaaa gtaaatgtcc
1141 atggaggagc tgtttctctg ggccatccaa tcgggatgtc tggagctcgg attgttgttc
1201 acttggctca tgccttgaag caaggagaat tcggtctggc tagtatttgc aatggaggag
1261 gaggggcttc cgccgtgctg attgagaagc tgtagacatc ttgttttagg agacagttcc
1321 acgtgacccg ctgaagtgaa ctaccccttg ggccagatta tattcaggat aagctatttc
1381 attttttatt attttctact aaaaattttt aaaaatcaca tccaaaaacc cattgaaatt
1441 gcaaataaaa atttctcctc ctttaatatt ttgtaaacag tcggatactc tactattgaa
1501 atatactgta ggtactagag gcatggctca gccgttaaga gcacttgttg ctacctgtgt
1561 ggtgcatggc tttaatccca gcacttggag acagaggcaa gtgcatcttt ctgagttaaa
1621 gttagcctgg tccacagagc tagtgccctg acagccaaga ctacacagag tagtagaaac
1681 tctgggggaa aaaaaaaaaa caaataaaaa aaaaa
//GenBank-Updates@genbank.bio.net (03/06/91)
LOCUS RATMTACAS 1480 bp ss-mRNA ORG 06-MAR-1991
DEFINITION Rat mitochondrial acetoacetyl-CoA thiolase mRNA, complete cds.
ACCESSION D00511
KEYWORDS acetoacetyl-CoA thiolase.
SOURCE Rat liver free polysome, cDNA to mRNA, clone RT2-6.
ORGANISM RT2-6
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1480)
AUTHORS Fukao,T., Kamijo,K., Osumi,T., Fujiki,Y., Yamaguchi,S., Orii,T. and
Hashimoto,T.
TITLE Molecular cloning and nucleotide sequence of cDNA encoding the
entire precursor of rat mitochondrial acetoacetyl-CoA thiolase
JOURNAL J. Biochem. 106, 197-204 (1989)
STANDARD full staff_entry
COMMENT The amino-terminal sequence of the mature enzyme was determined by
Edman degradation.
FEATURES Location/Qualifiers
CDS 21..1295
/note="mitochondrial acetoacetyl-CoA thiolase (EC 2.3.1.9)
precursor"
mat_peptide 111..1292
/note="mitochondrial acetoacetyl-CoA thiolase mature
protein"
misc_signal 1444..1449
/note="polyadenylation signal"
BASE COUNT 429 a 329 c 386 g 336 t
ORIGIN 20 bp upstream of the initiation codon.
1 gacaagcttt tccggtctcc atggctgccc tggcggttct acacggcgtc gtccgcaggc
61 ctctgctccg cgggctgctg caggaagtaa gatgcctggg acgaagttat gcatccaaac
121 ccactttgaa tgatgtggtt atagtgagtg ctacacgaac tcccattgga tccttcctgg
181 gcagccttgc ctctcagcca gccaccaagc ttggtactat tgcaattcag ggagccattg
241 aaaaggcagg gattccaaaa gaagaagtga aggaggtcta catgggcaac gtcatccaag
301 ggggagaagg acaggccccg accaggcaag ctacactggg tgcaggtcta cccattgcca
361 ctccgtgcac cacagtaaac aaggtgtgtg cctcaggaat gaaagccatc atgatggcct
421 ctcaaagtct tatgtgtgga caccaggatg tgatggtggc aggcggaatg gagagcatgt
481 caaatgtccc gtacgtaatg agcagaggag caacaccata cggtggggta aaacttgaag
541 acctgattgt gaaagacggg ctaaccgatg tctacaataa aattcatatg ggcaattgtg
601 ctgagaacac cgcgaagaag ctgagtatct cgcgggagga gcaggataag tacgccatcg
661 gctcttacac ccgaagtaaa gaggcgtggg atgcagggaa gtttgcaaat gagattacgc
721 ccatcaccat ctcagtgaaa ggtaaaccag acgtggtggt gaaggaagat gaagagtaca
781 agcgagttga cttcagtaaa gtgccaaagc tcaagacagt gttccagaaa gaaaacggca
841 cagtaacagc tgctaacgcc agcacactga acgacggagc agctgctgtg gttctcatga
901 ctgcagaggc agcccagcgg ctcaaggtta agccactggc acgaatcgca gcatttgctg
961 atgctgctgt agaccccatt gatttcccac tcgcacctgc atatgctgta cctaaggttc
1021 ttaaatatgc aggactgaaa aaagaagaca ttgccatgtg ggaagtaaat gaagcattca
1081 gtgtggttgt actagccaac attaaaatgc tggagattga ccctcaaaaa gtaaatgtcc
1141 atggaggagc tgtttctctg ggccatccaa tcgggatgtc tggagctcgg attgttgttc
1201 acttggctca tgccttgaag caaggagaat tcggtctggc tagtatttgc aatggaggag
1261 gaggggcttc cgccgtgctg attgagaagc tgtagacatc ttgttttagg agacagttcc
1321 acgtgacccg ctgaagtgaa ctaccccttg ggccagatta tattcaggat aagctatttc
1381 attttttatt attttctact aaaaattttt aaaaatcaca tccaaaaacc cattgaaatt
1441 gcaaataaaa atttctcctc ctttaaaaaa aaaaaaaaaa
//GenBank-Updates@genbank.bio.net (03/15/91)
LOCUS RATMTACAS 1480 bp ss-mRNA ORG 15-MAR-1991
DEFINITION Rat mitochondrial acetoacetyl-CoA thiolase mRNA, complete cds.
ACCESSION D00511
KEYWORDS acetoacetyl-CoA thiolase.
SOURCE Rat liver mitochondrial free polysome, cDNA to mRNA, clone RT2-6.
ORGANISM Mitochondrion Rattus norvegicus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1480)
AUTHORS Fukao,T., Kamijo,K., Osumi,T., Fujiki,Y., Yamaguchi,S., Orii,T. and
Hashimoto,T.
TITLE Molecular cloning and nucleotide sequence of cDNA encoding the
entire precursor of rat mitochondrial acetoacetyl-CoA thiolase
JOURNAL J. Biochem. 106, 197-204 (1989)
STANDARD full staff_entry
COMMENT The amino-terminal sequence of the mature enzyme was determined by
Edman degradation.
FEATURES Location/Qualifiers
CDS 21..1295
/note="mitochondrial acetoacetyl-CoA thiolase (EC 2.3.1.9)
precursor"
mat_peptide 111..1292
/note="mitochondrial acetoacetyl-CoA thiolase mature
protein"
misc_signal 1444..1449
/note="polyadenylation signal"
BASE COUNT 429 a 329 c 386 g 336 t
ORIGIN 20 bp upstream of the initiation codon.
1 gacaagcttt tccggtctcc atggctgccc tggcggttct acacggcgtc gtccgcaggc
61 ctctgctccg cgggctgctg caggaagtaa gatgcctggg acgaagttat gcatccaaac
121 ccactttgaa tgatgtggtt atagtgagtg ctacacgaac tcccattgga tccttcctgg
181 gcagccttgc ctctcagcca gccaccaagc ttggtactat tgcaattcag ggagccattg
241 aaaaggcagg gattccaaaa gaagaagtga aggaggtcta catgggcaac gtcatccaag
301 ggggagaagg acaggccccg accaggcaag ctacactggg tgcaggtcta cccattgcca
361 ctccgtgcac cacagtaaac aaggtgtgtg cctcaggaat gaaagccatc atgatggcct
421 ctcaaagtct tatgtgtgga caccaggatg tgatggtggc aggcggaatg gagagcatgt
481 caaatgtccc gtacgtaatg agcagaggag caacaccata cggtggggta aaacttgaag
541 acctgattgt gaaagacggg ctaaccgatg tctacaataa aattcatatg ggcaattgtg
601 ctgagaacac cgcgaagaag ctgagtatct cgcgggagga gcaggataag tacgccatcg
661 gctcttacac ccgaagtaaa gaggcgtggg atgcagggaa gtttgcaaat gagattacgc
721 ccatcaccat ctcagtgaaa ggtaaaccag acgtggtggt gaaggaagat gaagagtaca
781 agcgagttga cttcagtaaa gtgccaaagc tcaagacagt gttccagaaa gaaaacggca
841 cagtaacagc tgctaacgcc agcacactga acgacggagc agctgctgtg gttctcatga
901 ctgcagaggc agcccagcgg ctcaaggtta agccactggc acgaatcgca gcatttgctg
961 atgctgctgt agaccccatt gatttcccac tcgcacctgc atatgctgta cctaaggttc
1021 ttaaatatgc aggactgaaa aaagaagaca ttgccatgtg ggaagtaaat gaagcattca
1081 gtgtggttgt actagccaac attaaaatgc tggagattga ccctcaaaaa gtaaatgtcc
1141 atggaggagc tgtttctctg ggccatccaa tcgggatgtc tggagctcgg attgttgttc
1201 acttggctca tgccttgaag caaggagaat tcggtctggc tagtatttgc aatggaggag
1261 gaggggcttc cgccgtgctg attgagaagc tgtagacatc ttgttttagg agacagttcc
1321 acgtgacccg ctgaagtgaa ctaccccttg ggccagatta tattcaggat aagctatttc
1381 attttttatt attttctact aaaaattttt aaaaatcaca tccaaaaacc cattgaaatt
1441 gcaaataaaa atttctcctc ctttaaaaaa aaaaaaaaaa
//