GenBank-Updates@genbank.bio.net (03/15/91)
LOCUS OFA81MAA 1671 bp ds-DNA INV 15-MAR-1991
DEFINITION O.fallax macronuclear (81-MAC family, version A) DNA, region
SRRHGKH-a (partial).
ACCESSION M15835 M12073 M13033
KEYWORDS 81-MAC chromosome family; alternative DNA processing;
homolog of mitochondrial solute carriers.
SOURCE O.fallax (subkaryonide 3.5) macrocuclear DNA, region SRRHGKH-a,
clones pMA[83s,81].
ORGANISM Oxytricha fallax
Eukaryota; Animalia; Metazoa; Ciliophora; Polyhymenophora;
Spirotricha; Hypotrichida; Sporadotrichina; Oxytrichidae.
REFERENCE 1 (sites)
AUTHORS Herrick,G., Cartinhour,S., Dawson,D., Ang,D., Sheets,R., Lee,A. and
Williams,K.
TITLE Mobile elements bounded by C-4-A-4 telomeric repeats in Oxytricha
fallax
JOURNAL Cell 43, 759-768 (1985)
STANDARD full staff_review
REFERENCE 2 (bases 1617 to 1671)
AUTHORS Herrick,G.
JOURNAL Unpublished (1987) Univ. of Utah, Salt Lake City, Utah 84132
STANDARD full staff_entry
REFERENCE 3 (bases 1 to 1570)
AUTHORS Herrick,G., Hunter,D., Williams,K. and Kotter,K.
TITLE Alternative processing during development of a macronuclear
chromosomal family in Oxytricha fallax
JOURNAL Genes Dev. 1, 1047-1058 (1987)
STANDARD full staff_entry
REFERENCE 4 (bases 1 to 1616)
AUTHORS Herrick,G., Cartinhour,S.W., Williams,K.R. and Kotter,K.P.
TITLE Multiple sequence versions of the Oxytricha fallax 81-MAC alternate
processing family
JOURNAL J. Protozool. 34, 429-434 (1987)
STANDARD full staff_review
REFERENCE 5 (sites)
AUTHORS Williams,K.R. and Herrick,G.
TITLE Transcription analysis of a gene occupying the Oxytricha fallax
81-MAC chromosome common region and homologous to mitochondrial
solute carriers
JOURNAL Unpublished (1991)
STANDARD full staff_review
COMMENT
[Cell 43, 759-768 (1985)] sites; telomere-bearing element
description.
Draft entry and computer-readable sequence for [J. Protozool. 34,
429-434 (1987)] kindly provided
by G.Herrick, 12-DEC-1986.
This sequence is the final 1671 bp of that referred to as pRRHGKH-a
in [J. Protozool. 34, 429-434 (1987)].
The 81-MAC chromosome family is created by alternative processing
of the micronuclear DNA. The 81-MAC family consists of three sizes
of macronuclear chromosomes: 4.9 kb (MACI), 2.9 kb (MACII), and 1.6
kb (MACIII). Alternative processing results from alternative
choice of chromosome break/telomere-addition sites. The 1.6 kb
region in common between the three chromosomes bears a transcribed
open reading frame, the conceptual translation product of which is
homologous to solute carrier proteins from plants and metazoans.
There are three distinct micronuclear loci (sequence versions),vA,
vB, and vC. Alternative processing of each micronuclear version
independently gives rise to a I+II+III family of macronuclear
chromosomes. The open reading frame is conserved in all three
versions. Little is known about the structure of vB micronuclear
DNA (no clones), but vA and vC micronuclear DNAs bear
micronucleus-limited sequences called internal eliminated sequences
(IESs). vA and vC have small IESs in common, but in addition, vC
bears two large transposons, TBE1-1 and -2, which also are IESs.
IESs are precisely excised, leaving one short flanking direct
repeat in macronuclear DNA.
The micronuclear IES sequences (excision points annotated below)
can be found in separate entries: accession numbers M13041 and
M13042.
The telomeric sequence 'a [c(4)a(4)]4 c(4)' is added before
positions 1 and 34, and '[g(4)t(4)]4 g(4)' is added after position
157
FEATURES Location/Qualifiers
CDS join(complement(1464..1482),complement(1326..1411),
complement(1104..1255),complement(150..1008))
/note="protein homologous to mitochondrial solute
carriers"
/codon_start=1482
mRNA join(complement(1464..1511),complement(1326..1411),
complement(1104..1255),complement(61..1008),
complement(150..1008))
/note="protein homologous to mitochondrial solute
carriers"
mRNA join(complement(1464..1513),complement(1326..1411),
complement(1104..1255),complement(61..1008),
complement(150..1008))
/note="protein homologous to mitochondrial solute
carriers"
misc_feature 1..1570
/note="macronuclear DNA (MACIII); clone pMA83s (telomere
addition site)"
misc_feature 34..>1671
/note="macronuclear DNA (MACI); clone pMA81 (telomere
addition site)"
exon complement(61..1008)
/number=4
/note="protein homologous to mitochondrial solute
carriers"
misc_feature 262..266
/note="IES duplication sequence (excision site after 266)"
misc_feature 415..417
/note="TBE1-1 insertion target sequence (version C)"
intron complement(1009..1103)
/number=3
/note="protein homologous to mitochondrial solute
carriers"
exon complement(1104..1255)
/number=3
/note="protein homologous to mitochondrial solute
carriers"
intron complement(1256..1325)
/number=2
/note="protein homologous to mitochondrial solute
carriers"
exon complement(1326..1411)
/number=2
/note="protein homologous to mitochondrial solute
carriers"
misc_feature 1347..1349
/note="TBE1-2 insertion target sequence (version C)"
intron complement(1412..1463)
/number=1
/note="protein homologous to mitochondrial solute
carriers"
exon complement(1464..1511)
/number=1
/note="major transcription start site"
/note="protein homologous to mitochondrial solute
carriers"
exon complement(1464..1513)
/number=1
/note="minor transcription start site"
/note="protein homologous to mitochondrial solute
carriers"
misc_feature 1525..1528
/note="IES imperfect duplication sequence (excision site
after 1528)"
BASE COUNT 542 a 302 c 272 g 555 t
ORIGIN 144 bp upstream of EcoRI site.
1 aagtaactta ggactgtgat attaatatca actaagagta tatataaatt taatatttta
61 aaataggttt aaataacaaa taaatctcta ctttaacaaa aaagcatgtg ataatgctcg
121 atgttagaga caatttattt tttgaattct cagataattg cgagattttc ttttcctgaa
181 gggtgagcag ctcccatacc tccagtagct gtagtataag tagcaactaa ttttttattg
241 aaggcatctg tgtatggatc atgaatatcg taatcgatac cactttgata gtggaatctc
301 tatggttgcc agaattcttg atcatagtag ttttcgttat agtaatcgac caagaattga
361 gaaagataac aaatcaagaa caatctgagg aagtaggctt cacctcctgc atagaatgat
421 ccaaagttag acatccattt tgagttgcac tcgtacttta tgatcttgtt gaagcaatca
481 atcatgccgt tatatggcat ttgaccgttt ggtagtggtc tcatggtgtg tagtcttgtt
541 ctgatcatat caaaaggcat gcttacaaca gttccaatag caacagctac ggcagtaccc
601 cacaatctat taatccaatg aggaccaaag aagaagtagg agttctcctt gcaccaatcg
661 tagatgtttg tcattgatga acaaatagct gcaagcttaa aaccgttggc accagcacct
721 ctgaagagag caccttcttc agcaactttc gcaagtcctt aaattgtatt tgagtagttt
781 cttctggcag cttgtggata aagctcatca acctgcattc tattatagac gatatcgatg
841 ggatttgtca ctacaccagc aataaatcct ccgagtactc ctgctgctac caagaagtct
901 ggacgagcaa ctcttctggg atctttattt accttatcgt agaaataacc gaaggctgtt
961 actctagcag tagtgtaaca aactgttctg aagaagaatg tgtcgaaact ataccaatat
1021 aaattagcta aatttattgc aattcctcaa ttataaaatt gagagatgtt tttggataac
1081 ccaaattttg tttatacact tacgcagaat agaattttct gagtccaccg cctgctgcat
1141 aaatctcttc cataatttag aatggatacc aacctttttg ataaccaagg cctgagaagg
1201 tcttgccctc ttacagcatt tgagatctga ttctaaccat atcgagtggt taagtctata
1261 ttacattaag aatatgatta aatatcacaa agcatagtat attgtgcttt agggtattaa
1321 cttacagcgg catgagttat gagagcaata acattagcag cagcaaatct ttcatagttc
1381 atgcgttggt accttcttct ccagtactca actaaaaata aattttataa atcgatgatt
1441 cgatatttta ttgataaatt taccttccat ttaagtaggc atctttcttg attgcttatt
1501 ttaaaataat tttaataact ttatattaat tatgtttatt acaaattatt tcaattaagt
1561 tcattcaaaa ataaatctct gccaccttta aaatctatat ttaggttaac tcaagtcttc
1621 aaacaacact tctctgtgaa gaatcacttc ttaaaacacc agttttctac t
//GenBank-Updates@genbank.bio.net (03/15/91)
LOCUS OFA81MBAB 1959 bp ds-DNA INV 15-MAR-1991
DEFINITION O.fallax macronuclear (81-MAC family, version B) DNA, regions Kp-b
and pSRRHG-b.
ACCESSION M13031 M13032
KEYWORDS 81-MAC chromosome family; alternative DNA processing;
homolog of mitochondrial solute carriers.
SOURCE O.fallax (subkaryonide 3.5) macronuclear DNA, region Kp-b and
pSRRHG-b, clones pMA[82d,83,mu-1A1,XX-7,83].
ORGANISM Oxytricha fallax
Eukaryota; Animalia; Metazoa; Ciliophora; Polyhymenophora;
Spirotricha; Hypotrichida; Sporadotrichina; Oxytrichidae.
REFERENCE 1 (bases 1521 to 1959)
AUTHORS Herrick,G.
JOURNAL Unpublished (1987) Univ. of Utah, Salt Lake City, Utah 84132
STANDARD full staff_entry
REFERENCE 2 (bases 1902 to 1941)
AUTHORS Herrick,G., Hunter,D., Williams,K. and Kotter,K.
TITLE Alternative processing during development of a macronuclear
chromosomal family in Oxytricha fallax
JOURNAL Genes Dev. 1, 1047-1058 (1987)
STANDARD full staff_entry
REFERENCE 3 (bases 1 to 1520)
AUTHORS Herrick,G., Cartinhour,S.W., Williams,K.R. and Kotter,K.P.
TITLE Multiple sequence versions of the Oxytricha fallax 81-MAC alternate
processing family
JOURNAL J. Protozool. 34, 429-434 (1987)
STANDARD full staff_review
REFERENCE 4 (bases 1521 to 1782)
AUTHORS Hunter,D.J., Williams,K., Cartinhour,S. and Herrick,G.
TITLE Precise excision of telomere-bearing transposons during Oxytricha
fallax macronuclear development
JOURNAL Genes Dev. 3, 2101-2112 (1989)
STANDARD full staff_entry
REFERENCE 5 (sites)
AUTHORS Williams,K.R. and Herrick,G.
TITLE Transcription analysis of a gene occupying the Oxytricha fallax
81-MAC chromosome common region and homologous to mitochondrial
solute carriers
JOURNAL Unpublished (1991)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [J. Protozool. 34,
429-434 (1987)] kindly provided
by G.Herrick, 12-DEC-1986.
This sequence would be named 'pBSRRHGKp-B' by the convention in
[J. Protozool. 34, 429-434 (1987)].
The 81-MAC chromosome family is created by alternative processing
of the micronuclear DNA. The 81-MAC family consists of three sizes
of macronuclear chromosomes: 4.9 kb (MACI), 2.9 kb (MACII), and 1.6
kb (MACIII). Alternative processing results from alternative
choice of chromosome break/telomere-addition sites. The 1.6 kb
region in common between the three chromosomes bears a transcribed
open reading frame, the conceptual translation product of which is
homologous to solute carrier proteins from plants and metazoans.
There are three distinct micronuclear loci (sequence versions),vA,
vB, and vC. Alternative processing of each micronuclear version
independently gives rise to a I+II+III family of macronuclear
chromosomes. The open reading frame is conserved in all three
versions. Little is known about the structure of vB micronuclear
DNA (no clones), but vA and vC micronuclear DNAs bear
micronucleus-limited sequences called internal eliminated sequences
(IESs). vA and vC have small IESs in common, but in addition, vC
bears two large transposons, TBE1-1 and -2, which also are IESs.
IESs are precisely excised, leaving one short flanking direct
repeat in macronuclear DNA.
The telomeric sequence '[g(4)t(4)]4 g(4)' is added after position
1942 in the macronuclear DNA (MACIII) clone pMA83, and '[g(4)t(4)]4
g(4)t(2)' is the telomeric sequence added after position 1960 in
the macronuclear DNA (MACII or MACIII) clone pMA-mu-1A1.
FEATURES Location/Qualifiers
misc_feature <1..1941
/note="macronuclear DNA (MACIII) clone pMA83 (telomere
addition site)"
misc_feature <1..1959
/note="macronuclear DNA, clone pMA-mu-1A1 (telomere
addition site)"
CDS join(complement(1858..1876),complement(1720..1805),
complement(1497..1648),complement(543..1401))
/note="protein homologous to mitochondrial solute
carriers"
/codon_start=1876
mRNA join(complement(1858..1905),complement(1720..1805),
complement(1497..1648),complement((449.454)..1401))
/note="protein homologous to mitochondrial solute
carriers"
mRNA join(complement(1858..1907),complement(1720..1805),
complement(1497..1648),complement((449.454)..1401))
/note="protein homologous to mitochondrial solute
carriers"
exon complement((449.454)..1401)
/number=4
/note="major transcription start site"
/note="protein homologous to mitochondrial solute
carriers"
intron complement(1402..1496)
/number=3
/note="protein homologous to mitochondrial solute
carriers"
exon complement(1497..1648)
/number=3
/note="protein homologous to mitochondrial solute
carriers"
intron complement(1649..1719)
/number=2
/note="protein homologous to mitochondrial solute
carriers"
exon complement(1720..1805)
/number=2
/note="protein homologous to mitochondrial solute
carriers"
intron complement(1806..1857)
/number=1
/note="protein homologous to mitochondrial solute
carriers"
exon complement(1858..1905)
/number=1
/note="major transcription start site"
/note="protein homologous to mitochondrial solute
carriers"
exon complement(1858..1907)
/number=1
/note="minor transcription start site"
/note="protein homologous to mitochondrial solute
carriers"
BASE COUNT 636 a 325 c 356 g 641 t 1 others
ORIGIN 249 bp upstream of SphI site.
1 ggctctaaaa gaaaagatgt tcatgatctt gtcatggatc cttattcata gcaggggagt
61 ggnaggcttc acttgctggt tggtctcata tccctaagat ataattaaaa ctagacttca
121 ggttgctagg agccaagaat ttgcaaatta tagtcgatat ataagagatg gaggaatgat
181 tgaatgtgca aaatatatct ttaagaatga acatgggttt atgggatttt ggagagggtt
241 ctcagcatgc agtgcaagag cagtatttgc taattctttc atgtttgtgg cttatgaata
301 tgcttagaaa aaagcgagag gaattattga atgaataata gtggatttat caaagttttg
361 gaatgatata atgctttata atgtacttta tcaaagtaac ttaagactgt gatattaata
421 tcaactaaga ttatatataa atttaatatt tttaaatagg tttaaataac aaataaatct
481 ctactttaac aaaaaagcat gtgataatgc tctatgttag aagataattt atttctgaat
541 tctcagataa ttgcgagatt ttcttttcct gaagggtgag cagctcccat acctccagta
601 gctgtagtat aagtagcaac taatttttta ttgaaggcat ctgtgtatgg atcatgaata
661 tcgtaatcga taccactttg atagtggaat ctctatggtt gccagaattc ttgatcatag
721 tagttttcgt tatagtagtc taccaagaat tgagaaagat aacaaatcaa gaacaatctg
781 aggaagtagg cttcacctcc tgcatagaat gatccaaagt tagacatcca ctttgagtta
841 cactcgtact ttatgatctt gttgaagcaa tcaatcatgc cgttatatgg catttgaccg
901 tttggaaggg gtctcatggt gtgtagtctt gttctgatca tatcaaaagg catacttaca
961 acagttccaa tagcaacagc tacggcagtt ccccacaatc tattaatcca atgaggacca
1021 aagaagaagt aggagttctc cttgcaccaa tcgtagatgt tggtcattga tgaacaaata
1081 gctgcaagct taaaaccgtt ggcaccagca cctctgaaga gagcaccttc ttcagcaact
1141 ttggcaagtc cttaaattgt atttgagtag tttcttctgg cagcttgtgg ataaagctca
1201 tcaacctgca ttctattgta gacgatatcg atgggatttg tcactactcc agcaataaat
1261 cctccgagta ctcctgctgc taccaagaag tctggacgag caactcttct aggatcttta
1321 tttaccttat cgtagaaata accgaaggct gttactctag cagtagtgta acaaacagtt
1381 ctgaagaaga atgtgtcgaa actataccaa tataaattag ctaaatttat tgcaattcct
1441 caattataag gttgagagag ctttttggat aacccaaatt ttgtttataa acttacgcag
1501 aatagaattt tctgagtcca ccgcctgctg cataaatctc ttccataatt tagaatggat
1561 accaaccctt ttgataacca agaccagaga aggtcttgcc ctcttacagc atttaagatc
1621 tgattctaac catatcgagt ggttaagtct atattacatt aggaatatga ttaatcattg
1681 ctaataagat agaaactgtg ctaaaggata ttaacttaca gcagcatgag ttatgagagc
1741 aatgacattg gcagcagcaa atctttcata gttcatgcgt tggtaccttc ttctccagta
1801 ctcaactaaa aagcaatttt ataaatcgat gattcgatat tttattgata aatttacctt
1861 ccatttaagt aggcatcttt cttgattgct tattttaaaa taattataat aactttatat
1921 taattatgtt tattacaaat tatttcaatt aagctcatt
//GenBank-Updates@genbank.bio.net (03/15/91)
LOCUS OFA81MCAC 467 bp ds-DNA INV 15-MAR-1991
DEFINITION O.fallax macronuclear (81-MAC family) TBE1-2-less vC DNA region.
ACCESSION M25391
KEYWORDS 81-MAC chromosome family; alternative DNA processing;
homolog of mitochondrial solute carriers.
SOURCE O.fallax (subkaryonide 3.5) macronuclear DNA, region HGHK-c.
ORGANISM Oxytricha fallax
Eukaryota; Animalia; Metazoa; Ciliophora; Polyhymenophora;
Spirotricha; Hypotrichida; Sporadotrichina; Oxytrichidae.
REFERENCE 1 (bases 1 to 467)
AUTHORS Herrick,G.
JOURNAL Unpublished (1989) Univ. of Utah, Salt Lake City, Utah 84132
STANDARD full staff_entry
REFERENCE 2 (bases 1 to 467)
AUTHORS Hunter,D.J., Williams,K., Cartinhour,S. and Herrick,G.
TITLE Precise excision of telomere-bearing transposons during Oxytricha
fallax macronuclear development
JOURNAL Genes Dev. 3, 2101-2112 (1989)
STANDARD full staff_entry
REFERENCE 3 (sites)
AUTHORS Williams,K.R. and Herrick,G.
TITLE Transcription analysis of a gene occupying the Oxytricha fallax
81-MAC chromosome common region and homologous to mitochondrial
solute carriers
JOURNAL Unpublished (1991)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence [Genes Dev. 3, 2101-2112
(1989)] kindly provided by
G.Herrick, 31-OCT-1989.
This sequence would be named 'HGKH-c' by the conventions used in
the Herrick laboratory.
The 81-MAC chromosome family is created by alternative processing
of the micronuclear DNA. The 81-MAC family consists of three sizes
of macronuclear chromosomes: 4.9 kb (MACI), 2.9 kb (MACII), and 1.6
kb (MACIII). Alternative processing results from alternative
choice of chromosome break/telomere-addition sites. The 1.6 kb
region in common between the three chromosomes bears a transcribed
open reading frame, the conceptual translation product of which is
homologous to solute carrier proteins from plants and metazoans.
There are three distinct micronuclear loci (sequence versions),vA,
vB, and vC. Alternative processing of each micronuclear version
independently gives rise to a I+II+III family of macronuclear
chromosomes. The open reading frame is conserved in all three
versions. Little is known about the structure of vB micronuclear
DNA (no clones), but vA and vC micronuclear DNAs bear
micronucleus-limited sequences called internal eliminated sequences
(IESs). vA and vC have small IESs in common, but in addition, vC
bears two large transposons, TBE1-1 and -2, which also are IESs.
IESs are precisely excised, leaving one short flanking direct
repeat in macronuclear DNA.
The micronuclear IES sequence and the ends of TBE1-2 (excision
points annotated below) can be found in separate entries under
accession numbers M13039 and M13037.
FEATURES Location/Qualifiers
CDS join(complement(390..408),complement(252..337),
complement(29..180))
/note="protein homologous to mitochondrion solute
carriers"
/codon_start=408
/partial
mRNA join(complement(390..437),complement(252..337),
complement(29..180))
/note="protein homologous to mitochondrion solute
carriers"
/partial
mRNA join(complement(390..439),complement(252..337),
complement(29..180))
/note="protein homologous to mitochondrion solute
carriers"
/partial
intron complement(1..28)
/number=3
/note="protein homologous to mitochondrion solute
carriers"
/partial
exon complement(29..180)
/number=3
/note="protein homologous to mitochondrion solute
carriers"
intron complement(181..251)
/number=2
/note="protein homologous to mitochondrion solute
carriers"
exon complement(252..337)
/number=2
/note="protein homologous to mitochondrion solute
carriers"
misc_feature 273..275
/note="TBE1-2 insertion target sequence (excision site
after 275)"
intron complement(338..389)
/number=1
/note="protein homologous to mitochondrion solute
carriers"
exon complement(390..437)
/number=1
/note="major transcription start site"
/note="protein homologous to mitochondrion solute
carriers"
exon complement(390..439)
/number=1
/note="minor transcription start site"
/note="protein homologous to mitochondrion solute
carriers"
misc_feature 451..454
/note="IES imperfect duplication sequence (excission site
after 454)"
BASE COUNT 155 a 79 c 65 g 168 t
ORIGIN
1 ataagccaaa ttttgtttat acacttacgc agaatagaat tttttgagtc cacctcctgc
61 tgcataaatc tcttccataa tttagaatgg ataccaaccc ttttgataac caaggcctga
121 gaaggtcttg ccctcttaca gcatttgaga tctgattcta accatatcga gtggttaagt
181 ctatattaca tttagaatat gattaatcat tgctaataag atagaaaatg tgctatagaa
241 tactaactta cagcagcatg agttatgaga gcaatgacat tagcagcagc aaatctttca
301 tagttcatgc gttggtacct tcttctccag tactcaacta aaacgaaatt ttagaaatca
361 atgattcgat attttattga taaatttacc ttccatttaa gtaggcatct ttcttgattg
421 cttattttaa aataattata ataactttat attaattatg tttatta
//GenBank-Updates@genbank.bio.net (03/16/91)
LOCUS OFA81MAB 397 bp ds-DNA INV 16-MAR-1991
DEFINITION O.fallax macronuclear (81-MAC family, version A) DNA, region Gp-a.
ACCESSION M13034
KEYWORDS 81-MAC chromosome family; alternative DNA processing;
homolog of mitochondrial solute carriers.
SOURCE O.fallax (subkaryonide 3.5) macronuclear DNA, region Gp-a, clone
pMA81.
ORGANISM Oxytricha fallax
Eukaryota; Animalia; Metazoa; Ciliophora; Polyhymenophora;
Spirotricha; Hypotrichida; Sporadotrichina; Oxytrichidae.
REFERENCE 1 (bases 1 to 397)
AUTHORS Herrick,G.
JOURNAL Unpublished (1987) Univ. of Utah, Salt Lake City, Utah 84132
STANDARD full staff_entry
REFERENCE 2 (bases 1 to 397)
AUTHORS Herrick,G., Hunter,D., Williams,K. and Kotter,K.
TITLE Alternative processing during development of a macronuclear
chromosomal family in Oxytricha fallax
JOURNAL Genes Dev. 1, 1047-1058 (1987)
STANDARD full staff_review
REFERENCE 3 (bases 1 to 397)
AUTHORS Herrick,G., Cartinhour,S.W., Williams,K.R. and Kotter,K.P.
TITLE Multiple sequence versions of the Oxytricha fallax 81-MAC alternate
processing family
JOURNAL J. Protozool. 34, 429-434 (1987)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [J. Protozool. 34,
429-434 (1987)] kindly provided
by G.Herrick, 12-DEC-1986.
This sequence is referred to as Gp-a in [J. Protozool. 34, 429-434
(1987)].
The 81-MAC chromosome family is created by alternative processing
of the micronuclear DNA. The 81-MAC family consists of three sizes
of macronuclear chromosomes: 4.9 kb (MACI), 2.9 kb (MACII), and 1.6
kb (MACIII). Alternative processing results from alternative
choice of chromosome break/telomere-addition sites. The 1.6 kb
region in common between the three chromosomes bears a transcribed
open reading frame, the conceptual translation product of which is
homologous to solute carrier proteins from plants and metazoans.
There are three distinct micronuclear loci (sequence versions),vA,
vB, and vC. Alternative processing of each micronuclear version
independently gives rise to a I+II+III family of macronuclear
chromosomes. The open reading frame is conserved in all three
versions. Little is known about the structure of vB micronuclear
DNA (no clones), but vA and vC micronuclear DNAs bear
micronucleus-limited sequences called internal eliminated sequences
(IESs). vA and vC have small IESs in common, but in addition, vC
bears two large transposons, TBE1-1 and -2, which also are IESs.
IESs are precisely excised, leaving one short flanking direct
repeat in macronuclear DNA.
The telomeric sequence ('g4t4)4 g4t' is added to position 397.
FEATURES Location/Qualifiers
BASE COUNT 125 a 40 c 72 g 158 t 2 others
ORIGIN Unreported.
1 ttattctatt catatttgct ccaaggcctg agttagtgtg ttmcatagtt tgattgtaaa
61 ttaatagatg cactactctt gtttgaagat tcttttatag gtggagagta cgaargatta
121 gaattgttct aagctgatga ttgagaagaa gttattgtta tgataggcca ttttcatatt
181 ttaaatgaaa gaattgcctg tagattggta ggaattattg tttgaaagac cttaagacac
241 tttaagtggt taggtctatg gaataagata tttatttgtt tgcatttaac ttcctaactg
301 agaaagtttc taggtctaag attgttaaga ttatggataa ttcatttttg tttagaaata
361 ttttaaattc aaatttataa ctactataac tacacat
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