GenBank-Updates@genbank.bio.net (03/16/91)
LOCUS ECOMINB 2400 bp ds-DNA BCT 16-MAR-1991
DEFINITION E.coli minicell (minB) locus encoding minC, minD, and minE genes,
(required for the proper placement of the division septum) complete
cds.
ACCESSION J03153
KEYWORDS MinC protein; MinD protein; MinE protein; minC gene; minD gene;
minE gene.
SOURCE E.coli (strain K-12) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 2400)
AUTHORS de Boer,P.A., Crossley,R.E. and Rothfield,L.I.
TITLE A division inhibitor and a topological specificity factor coded for
by the minicell locus determine proper placement of the division
septum in E. coli
JOURNAL Cell 56, 641-649 (1989)
STANDARD full staff_review
REFERENCE 2 (sites)
AUTHORS Labie,C., Bouche,F. and Bouche,J.-P.
TITLE Minicell-forming mutants of Escherichia coli: Suppression of both
DicB- and MinD-dependent division inhibition by inactivation of the
minC gene product
JOURNAL J. Bacteriol. 172, 5852-5855 (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Cell 56, 641-649
(1989)] kindly provided
by P.A.J.de Boer, 09-FEB-1989.
FEATURES Location/Qualifiers
CDS 196..891
/note="MinC protein /nomgen='minC'"
/codon_start=196
mutation 224..224
/note="replace(224,'a')"
/citation=J. Bacteriol. 172, 5852-5855 (1990)
mutation 378..378
/note="replace(378,'a')"
/citation=J. Bacteriol. 172, 5852-5855 (1990)
mutation 677..677
/note="replace(677,'a')"
/citation=J. Bacteriol. 172, 5852-5855 (1990)
mutation 707..707
/note="replace(707,'a')"
/citation=J. Bacteriol. 172, 5852-5855 (1990)
mutation 745..745
/note="replace(745,'t')"
/citation=J. Bacteriol. 172, 5852-5855 (1990)
mutation 782..782
/note="replace(782,'t')"
/citation=J. Bacteriol. 172, 5852-5855 (1990)
mutation 844..844
/note="replace(844,'t')"
/citation=J. Bacteriol. 172, 5852-5855 (1990)
CDS 915..1727
/note="MinD protein /nomgen='minD'"
/codon_start=915
mutation 1471..1471
/note="replace(1471,'a')"
/citation=J. Bacteriol. 172, 5852-5855 (1990)
mutation 1699..1699
/note="replace(1699,'a')"
/citation=J. Bacteriol. 172, 5852-5855 (1990)
CDS 1731..1997
/note="MinE protein /nomgen='minE'"
/codon_start=1731
mutation 2028..2028
/note="replace(2028,'a')"
/citation=J. Bacteriol. 172, 5852-5855 (1990)
BASE COUNT 645 a 563 c 587 g 605 t
ORIGIN 194 bp upstream of FokI site; 25.6 minutes on E.coli map.
1 acgacggcaa tgggttgatt gacaagggta ttttttaagc tatgaatcag cgccatttat
61 cacagaatag acttttactc tgaataaatg ggagggtgac ttgcctcaat ataatccaga
121 ctataacatg ccttatagtc ttcggaacat catcgcgcgc tggcgatgat taatagctaa
181 ttgagtaagg ccaggatgtc aaacacgcca atcgagctta aaggcagtag cttcacttta
241 tctgtggttc atctgcatga ggcagaacct aaggttatcc atcaggcgct ggaagacaaa
301 atcgctcagg cccccgcatt tttaaaacat gcccccgttg tactcaacgt cagtgcactg
361 gaagacccgg taaactggtc agcgatgcat aaggcggttt cggcaaccgg tttgcgggtt
421 attggcgtaa gtggctgcaa agatgcgcaa cttaaagccg aaattgaaaa gatggggctg
481 cctatcctga cggaaggaaa ggaaaaagcg ccacgtccag ctcccacacc gcaggctcca
541 gcgcaaaata caacgccggt cacaaaaacg cgtttaatag ataccccggt gcgttccggt
601 cagcgtattt atgctccaca atgtgatctg attgttacaa gccacgttag cgctggggcc
661 gaattgattg ccgatgggaa cattcatgtc tatggcatga tgcgcggtcg tgcgctggca
721 ggggcaagtg gtgaccggga aacgcaaata ttttgtacga acctgatggc ggaactggtg
781 tccatcgcag gtgaatactg gctgagtgat caaatcccag cagaatttta tggcaaagcg
841 gcgcgactgc agttagtcga aaacgctttg accgttcaac cgttaaattg atcccttttt
901 aacaaggaat ttctatggca cgcattattg ttgttacttc gggcaaaggg ggtgttggta
961 agacaacctc cagcgcggcc atcgccactg gtttggccca gaagggaaag aaaactgtcg
1021 tgatagattt tgatatcggc ctgcgtaatc tcgacctgat tatgggttgt gaacgccggg
1081 tcgtttacga tttcgtcaac gtcattcagg gcgatgcaac gctaaatcag gcgttaatta
1141 aagataagcg tactgaaaat ctctatattc tgccggcatc gcaaacacgc gataaagatg
1201 ccctcacccg tgaaggggtc gccaaagttc ttgatgatct gaaagcgatg gattttgaat
1261 ttatcgtttg tgactccccg gcagggattg aaaccggtgc gttaatggca ctctattttg
1321 cagacgaagc cattattacc accaacccgg aagtctcctc agtacgcgac tctgaccgta
1381 ttttaggcat tctggcgtcg aaatcacgcc gcgcagaaaa tggcgaagag cctattaaag
1441 agcacctgct gttaacgcgc tataacccag gccgcgtaag cagaggtgac atgctgagca
1501 tggaagatgt gctggagatc ctgcgcatca aactcgtcgg cgtgatccca gaggatcaat
1561 cagtattgcg cgcctctaac cagggtgaac cggtcattct cgacattaac gccgatgcgg
1621 gtaaagccta cgcagatacc gtagaacgtc tgttgggaga agaacgtcct ttccgcttca
1681 ttgaagaaga gaagaaaggc ttcctcaaac gcttgttcgg aggataagtt atggcattac
1741 tcgatttctt tctctcgcgg aagaaaaaca cagccaacat tgcaaaagaa cggctgcaga
1801 ttattgttgc tgaacgccgt cgcagcgatg cagaaccgca ttatctgccg cagttgcgta
1861 aagatattct tgaggtcatt tgtaaatacg tacaaattga tcctgagatg gtaaccgtac
1921 agcttgagca aaaagatggc gatatttcta ttcttgagct gaacgtgacc ttaccggaag
1981 cagaagagct gaaataagcc cgctgtaaaa gcgcatttat cttcaaggca gagttatctc
2041 tgccttgagt ttttcatccc tctcatccac gttgtggtaa agcggcgagt attcttgctg
2101 atactcctca ttgctatttc cacctccccc tttacacctt aaggctgttc ccacgcacaa
2161 aacaaaatgt ttatgcctgg ttgagtaaat aaccttattg ttagtatgga tatactggga
2221 gatgataacc ccgcaatttc attattcatt gttctcccat aggatgaggt ttcccgccgt
2281 aactggcggg ctttttttgc cccagaattt tccctttcaa catcctgtaa acgaaaactg
2341 cgccgaagcg cagtttaatc gttgatggtc agaacgacca gttcacacca gccaccgcgt
//