GenBank-Updates@genbank.bio.net (03/16/91)
LOCUS ECHPRTBCDE 8524 bp ds-DNA BCT 16-MAR-1991
DEFINITION Erwinia chrysanthemi prt locus encoding inhibitor (inh) and
protease (prtB, prtC) and prtD, prtE, and prtF genes, complete cds.
ACCESSION M60395 J05627
KEYWORDS inhibitor; protease B; protease C; prtB gene; prtC gene; prtD gene;
prtE gene; prtF gene.
SOURCE Erwinia chrysanthemi (strain B374) DNA, clone pRUW1.
ORGANISM Erwinia chrysanthemi
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 8524)
AUTHORS Delepelaire,P. and Wandersman,C.
TITLE C-terminal secretion signal in protease secreted by gram negative
bacteria
JOURNAL J. Biol. Chem. (1991) In press
STANDARD full staff_entry
FEATURES Location/Qualifiers
RBS 327..329
/gene="inh"
CDS 335..697
/product="inhibitor"
/gene="inh"
/codon_start=335
sig_peptide 335..391
/gene="inh"
/codon_start=335
mat_peptide 392..694
/product="inhibitor"
/gene="inh"
/codon_start=392
RBS 699..704
/gene="prtD"
CDS 714..2441
/gene="prtD"
/codon_start=714
RBS 2445..2447
/gene="prtE"
CDS 2455..3801
/gene="prtE"
/codon_start=2455
RBS 3807..3809
/gene="prtF"
CDS 3814..5202
/gene="prtF"
/codon_start=3814
sig_peptide 3814..3882
/gene="prtF"
/codon_start=3814
mat_peptide 3883..5199
/gene="prtF"
/codon_start=3883
RBS 5402..5407
/gene="prtB"
CDS 5414..6859
/product="protease"
/gene="prtB"
/codon_start=5414
mat_peptide 5459..6853
/product="protease"
/gene="prtB"
/codon_start=5459
RBS 7003..7005
/gene="prtC"
CDS 7010..8449
/product="protease"
/gene="prtC"
/codon_start=7010
BASE COUNT 1904 a 2186 c 2611 g 1823 t
ORIGIN
1 gaattcgttc cggatcttcg ctttgtggat aatttctcgg gaaaaggaaa cgaagtggtg
61 ttgaattggg atagtcagtc tcatcaaacc aatatgtggt tgcatttaag cgggcacgag
121 acggctgatt ttctggtcaa cattgtcggt gcggcattac aaccgtcgga tgttattgtt
181 taaaaataac gggaacgctc ccgctatttt cgggtttgtt gaataacggc aggtgttttc
241 tcttatcgga aatatgcctg ccgttatcaa catatttaac cagatggttt caattattaa
301 acgcgttcac tgatgaccga atgatgggaa tatcatgaaa cagcttatta ttgcgacatt
361 attgagtgca ctgagtggag gatgtatggc gagcagtctg agattgccgt ccgcggcgga
421 actgagcggg cagtgggtat tgagcggcgc agagcagcac tgtgatatcc ggttgaacac
481 cgatgtgctt gacggcacga cctggaagct ggctggcgac acggcgtgcc tgcaaaaatt
541 gctgccggaa gcgccggtcg gttggcggcc gacgcccgac ggcttgacgt tgacgcaggc
601 agacggctcg gcggtggcgt ttttcagccg caaccgcgat cgctacgaac ataagctggt
661 tgacggcagc gttcgcaccc tgaaaaagaa agcctaaggg agagagccct gccgtgaatg
721 cttcctctga gcgagatcgt tcgttgttcg gcgtgctgcg ccagttccgc cgcagtttct
781 ggagcgtcgg gattttcagc gccgtcatca atgtgctgat gctggcgcct tcggtatata
841 tgctgcaggt gtatgaccgg gtgctggcgt ccggcaacgg tataaccctg ctgatgctga
901 cgttgctgat ggcgggactg tgcgccttta tgggcgcgct ggagtgggtt cgtagtctgt
961 tggtggtgag gctgggcacc cgtatcgatc tggcgctcaa tcaggatgtc ttcaatgcgg
1021 ccttcgcccg taatctggag gccggcgatg gccgcgccgg gctggcgctg accgacctga
1081 cgctgctgcg ccagttcatc accggcaatg cgctgttcgc cttcttcgat gtgccctggt
1141 ttccgctgtt tttgctggtg ctgtttttgc tgcacccctg gctggggatg ctggcgctgg
1201 gcggcaccgt ggtgcctggt ggcgttggcc tggctgaacc agcatctgac caatcaaccg
1261 ctggcggaag caatcagcaa tcgcaacagg cgacccatct ggctgacgct cagttgcgca
1321 atgccgacgt tattgaagca atggggatgc tgggcaacct gcgtcggcgc tggctggcgc
1381 gtcattaccg ttttatttcc ctgcagaatc tggccagcga gcgtgccgcc gcggtaggcg
1441 gtgcgtccaa atacagccgt attgcgttgc agtcgttgat gctggggctg ggagcattgc
1501 tggcgatcga cggcaagatc acgccgggga tgatgatcgc cggttcgatt ctggttggcc
1561 gggtactgag cccgatcgat cagttaatcg gggtatggaa acagtggagc agcgcgcgca
1621 ttgcctggca gcgcctgacc cggctgatcg ccgcctatcc gccgcgcccg gcggcaatgg
1681 cgctgcctgc gccggaaggg catctcagtg tggaacaggt gtcgctgcgc acggcgcagg
1741 gcaatacccg actgcagaac atccatttct cgttgcaggc tggcgaaacg ctggtgattc
1801 tcggcgcgtc cggttccggt aaatcctcgc tggcgcgctt gctggtcggc gcgcagtcgc
1861 cgacgcaagg caaagtgcgt ctggatggcg ctgatttgaa tcaggtggac aaaaacacgt
1921 ttggtccgac catcggctat ttgccgcagg atgtgcagct gtttaagggc tcgctggcgg
1981 aaaacatcgc ccgttttggc gatgccgatc cggaaaaagt ggtggcggcc gcaaaactgg
2041 cgggggtaca tgaactgatt ttgtcgctac ccaatggcta tgacaccgaa ttgggcgatg
2101 gcggcggcgg tttgtccggc ggccagcggc agcgtatcgg tctggcgcgt gccatgtacg
2161 gcgatccttg cctgctgatt ctggatgaac cgaacgccag ccttgacagc gaaggcgatc
2221 aggccttgat gcaggcgatt gtcgcgttgc agaaacgcgg cgccaccgtg gtgttgatta
2281 cccatcgccc ggcgctgacg acgctggcgc aaaaaattct gatcctccat gagggccagc
2341 agcagcgtat gggactggcg cgggatgtgc tgacggaact gcaacagcgc agcgccgcca
2401 atcaggcccg gatgaacccc acggccgcaa tgccgcaatg acagggatct cgtgatgaca
2461 ggcatggata taaccactca ggatgaattg aacgaagcag caatgcgcga tcgcgccagc
2521 cgggatgaag agcgcgcgtt acggctgggc tggtggctgg tgctcgccgg ttttggcggt
2581 tttctgctgt gggccttgct ggcgccgttg gataaagggg tggcggtgca aggcaatgtg
2641 gtggtgtccg gtaatcgcaa ggtgatccaa cacatgcagg gcggcattgt cgaccggatt
2701 caggtgaaag acggcgaccg cgttgccgcg ggtcaggtgt tgctgacgct gaatgcggta
2761 gatgcccgta ccaccagcga agggctgggc tcccaatacg atcaattgat tgctcgtgaa
2821 gcccgactgc tggcggaaca acgtaatcaa tcgtcgctgg cggcgacgcc ccggctgact
2881 caggcgcgcc agcggccgga aatggccgcc atcattgcgt tgcaggaaga tctattgcgt
2941 agccggcagc agtcgctaaa gctggaaata gacggagtgc gcgccagcat cgatggcctg
3001 gagacgtcgc ttggcgcatt gcaaaaggtg atgagcagca aacagtccga gcaggcgacg
3061 ttgagccagc agttgcaggg actgcggccg ctggcggcgg ataactatgt tccgcgtaat
3121 aagatgctgg aaacggagcg cttgtttgcg caggtcagcg gcgaactggc ccagaccagc
3181 ggcgaggtag ggcgaacccg gcgtgatatt cagcagcaaa aattgcgcat cgcccagcgt
3241 cagcaggaat acgacaaaga ggtcaacagc gaattgtccg acgtacaggc caaactgaat
3301 gaggtgatca gtcagcgcga gaaggcggat ttcaatctgg ccaacgtgca ggtccgtgca
3361 ccggttgccg gcaccgtggt ggatatgaag atcttcaccg aaggcggggt gatcgccccc
3421 gggcaggtca tgatggacat cgttcccgaa gaccagccgc tgctggtgga tgggcgcatt
3481 ccggtggaaa tggtggacaa ggtgtggtcc gggctgccgg tggaactgca gttcaccgcg
3541 tttagccaaa gcaccacgcc gcgagtgccg ggaacggtga ccctgctttc cgctgaccgg
3601 ctggtggacg aaaaggacgg cacgccgtac tacggtttgc gcattcaggt gagcgaagaa
3661 ggcaagcgtt cgttgcacgg gctggaaatt aaacctggta tgccggtgca ggggtttgtc
3721 cgcaccggag agcgttcgtt tatcaattat ctgtttaaac ccctgatgga tcgcatgcat
3781 ctggcgttga cggaagagta acggaaggaa gcaatgcgaa ggaaagcggt actactgacg
3841 gtcgtgttat ctctgtcggg cggcagcgcc caggcgatgg ggctgctgga tgcatgggaa
3901 ctggcgctgc gtaatgacgc acagctgcgg gcggccggtt tcgaacgaga cgccgggcag
3961 gaagaggtgg cgatcggccg cgccgggttg ctgccgtcgc tgcaatatac ctatggcgct
4021 aattacagtc attccaaggt gacgcaacgc gatcggacgc tgaataacac gactaaacgt
4081 gactacgata actacgtatc gacgttgacg ctgcgtcagc cgctgctgga ctatgccgcc
4141 tgggcgcgct accagcaggg cgtcacccgc aaactgatgg ccgatcagcg atttcgcgat
4201 cgcagtcagg acctgatggt gcgtttgtac cagtcctgga gcgaggcatt gctggcgcag
4261 gagaaattga tgctgctgga tgcgcagcgc cgtgcctatc aggagcagtt ggcgctgaac
4321 cgtcgcttat tggcggcggg ggaaggcacg caaaccgacc tgcgggaaac ggaggcgcgt
4381 tacaccgtca ctgaagccca gcgtatcgaa caggaagata cgctggatgc cgctatgacc
4441 gatctggaaa acatgatggg ttcgccgttg caaattcagg atctgtcgcc gttggcgctg
4501 gatacgttgc cggataatgt caccgaaaat cgctcgctgt cgcagtggcg cgaactgacg
4561 gtgcggcaca acgcaaagct ggcggtacag cgtgaaaacg tcgattacag ccgctacgaa
4621 attgagcgta atcgcgccgg ccatctgccg acattggatt tggtggcgtc cacccgcaac
4681 agcttgtcgg agtctgaata caactacaac cagaaatacg acacccagac cgtcggtttg
4741 caggtgcgcg tgccgctgta ttccggcggc gccgtttcgg cctcgatgcg tcaggcagcg
4801 gcggaatacc agcaaagtca ggcggagctg gataaccaga cccggcagac ctttgcggaa
4861 ttacgccgtc agttcaacct gtgcgccaat ggggccgcca aaatccgagc ctggcagatg
4921 agcgtggccg ccgctgagga agcgatccgc gcgacgcggc agagcgtcgc cggcggcgag
4981 cgcatcaatc tggacgtgct gatggcggaa caggagtggt ataacgcccg ccgcgagctg
5041 acggaagtca aatatcgctg gttacaggcc tggctgaatt tgcgctatac cgccggaacg
5101 ctgaatgagc aggatatgat gcaattggct gcctggtttc aatctgcgcc tgtaataaat
5161 aaaaccggaa taaacgcggc caccggtaat aaaacgaatt agcggtaacc tttggcgtta
5221 gttggttgtg gtgtctatga aaactgatgg ttttctctac aaaaattgat tgcagtttcc
5281 acgaaatttc tttcgcagcc cgctgcgaga gatgggtatg tcattaaatt agccgtgatg
5341 ataattgccg gcgatttaat ggcgaaacaa ggcattctga cagtaatgcg tcagacttta
5401 tggaagaagt gatatgcaac aaaacgaaaa agcttcgtta aatacctctg ccgctgccgc
5461 caaggcggga agttataccg gctatgccga tgtgtatgat ttttggtatt accatcagcg
5521 cggcgacggt ctgactgtca atggtaaacc gtcttatacc accgataagg cggtgagcga
5581 gggattaacg cggccgcata ccacctggaa cggtgataac gtatttggca aagcggcaaa
5641 tctgacctac tcgtttctga acacgtttag ctcgacgcct aacggtcata ccggcccggt
5701 aaaatttacg ccggtgcaga tgcagcaggc aaaactgtca ttacagtcct gggcggatgc
5761 ggcgaacctg acgtttaccg aagtcagccc caatcaaaaa gccaatatca cattcgccaa
5821 ctatacgcgc aatgccgacg gcagcctgaa tactgatacc caggcttatg cggcttatcc
5881 aggtacgcat ccggtatccg gcagcgcctg gttcaactac aaccagtcca gcattcgcaa
5941 cccggatacc gatgagtatg gacgtcattc gttcacgcat gaaatcggtc atgcgctggg
6001 gctgagccat ccggcggagt acaacgccgg cgaaggcgat attagctaca aaaacagcgc
6061 tgcctatgcg gaagactccc gtcagttcag catcatgagc tactgggaag tggaaaacac
6121 cggcggtgac ttcaaaggtc actactcggc aggtccgctg atggatgata tcgcggcgat
6181 ccagaagctg tacggcgcca acatgaccac ccgtaccggc gataccgtgt atggcttcaa
6241 ctccaatacc gatcgtgatt tctataccgc gaccaacagt agcaaggcgt tggttttctc
6301 ggtatgggat gccggcggca ccgacacctt cgacttctcc ggttacagca acaaccagcg
6361 tatcaatctg aatgaaggct cgttttcgga cgtgggcggc ctgaaaggca acgtgtcgat
6421 cgcgcacggc gtgaccatcg agaatgctat tggcggttcc ggcaacgaca ttctgattgg
6481 taacggcgca gataacatac tgcagggcgg cgcgggcgac gacgtgctgt acggcagcac
6541 tggcgcggat acgctgaccg gcggcgccgg acgcgatatc ttcgtctacg gcagcggtca
6601 ggattccacc gtgtcggcat acgactggat taccgatttc cagaccggta tcgacaagat
6661 cgatctgtcg gcgttccgca acgaaggcca actgagcttt gtgcaggacc agttcaccgg
6721 caaaggtcag gaagtgatgc tgcagtggga tgcggccaac agcacgacca acttgtggct
6781 gcatgaagcg ggtcacagct cagtggattt tctggtacgc attgtcggcc agaccgcgca
6841 atccgatatt attgtgtaat caaccactga aaatattaaa cagggtgggt taaccatcct
6901 gtttaataag atttccacgg gaatcttatt atcatgtcat tctggcaggg cgttgctcgt
6961 cctgaataaa ggatgacagt attcttttat cgtatttcca gaggatttta tgggaaaaaa
7021 tctgtcttta cgtcaagatg acgctcaaca cgcattatcc gcgaatacca gcagcgcgta
7081 taattccgtt tatgattttc tgcgttacca cgatcgtggc gatggcctga cggtcaatgg
7141 caaaacatcc tattccatcg atcaggccgc ggcgcaaatt acccgggaaa atgtcagctg
7201 gaatggaacc aatgtatttg gcaagtcggc caatctgacg tttaaatttc tgcagtcggt
7261 cagctccatt ccatccggcg ataccggttt tgtgaaattt aatgcagagc aaattgagca
7321 ggcgaaactg tcgctgcaat cctggtcgga cgtagccaat ctgacgttta ccgaagtaac
7381 gggaaataaa tctgctaata tcacctttgg taactatacg cgtgatgcga gtggcaacct
7441 ggattacggt actcaagcct atgcctatta tccgggtaat tatcaggggg cgggcagcag
7501 ctggtacaac tataaccagt ctaatatccg taatccgggg tcggaagaat atggccgtca
7561 gacctttacc cacgagattg gtcatgcgct tggcctggcc catccgggtg agtacaacgc
7621 cggcgaaggt gacccgagct ataatgacgc ggtgtatgcg gaagatagct atcaattcag
7681 tatcatgagc tactggggcg agaatgaaac cggtgccgac tacaacggtc actatggcgg
7741 cgcgccgatg attgacgaca tcgcggcgat tcagcggctg tatggcgcca acatgaccac
7801 ccgtaccggt gatagcgtgt atggtttcaa ttccaatacc gatcgtgatt tctatactgc
7861 gaccgacagc agtaaagcac tgattttctc agtatgggat gccggcggca ccgacacctt
7921 cgacttctcc ggctatagca acaaccagcg tatcaatctg aatgaaggct cgttttcgga
7981 cgtgggcggc ctgaaaggca acgtgtcgat cgcgcacggc gtgaccatcg aaaacgccat
8041 tggcggttcc ggcaacgata ttctggtggg caacagcgca gataacatac tgcagggcgg
8101 cgcgggcaat gacgtgctgt acggtggtgc cggcgcggat acgctgtacg gcggcgccgg
8161 gcgtgatacg ttcgtttacg gcagcggtca ggattccacc gtggcagcgt atgactggat
8221 tgccgatttc cagaaaggca ttgataaaat cgacctgtcg gcgttccgca acgaaggtca
8281 actgagcttt gtgcaggatc agttcaccgg caaaggtcag gaagtgatgc tgcagtggga
8341 tgcggccaac agcatcacca acctgtggtt gcatgaagca gggcacagct cggtagactt
8401 cctggtacga attgtcggtc aggcggcaca gtccgatatt attgtgtaat gatattctga
8461 aaatattgca cagggcagtt tatctgccct gtttaatatt ccattctgca ttagggtaga
8521 attc
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