GenBank-Updates@genbank.bio.net (03/16/91)
LOCUS TOBGLA13B 4482 bp ds-DNA PLN 16-MAR-1991
DEFINITION Nicotiana tabacum glucan beta-1,3-glucanase gene, complete cds.
ACCESSION M60402
KEYWORDS beta-1,3-glucanase; glucan beta-1,3-glucanase.
SOURCE Tobacco (strain Havana 425) leaf DNA, clone lambda-GLA.
ORGANISM Nicotiana tabacum
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Asteridae; Solanales; Solanaceae.
REFERENCE 1 (bases 1 to 4482)
AUTHORS Sperisen,C., Ryals,J. and Meins,F.Jr..
TITLE Comparison of cloned genes provides evidence for intergenomic
exchange of DNA in the evolution of a tobacco beta-1,3-glucanase
gene family
JOURNAL Proc. Natl. Acad. Sci. U.S.A. (1991) In press
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS join(1464..1554,2344..3365)
/EC_number="3.2.1.39"
/product="glucan beta-1,3-glucanase"
/gene="glucanase GLA"
/codon_start=1464
sig_peptide join(1464..1554,2344..2348)
/gene="glucanase GLA"
/codon_start=1464
mRNA join(1448..1554,2344..3820)
/note="major transcription start site"
/product="glucan beta-1,3-glucanase"
/gene="glucanase GLA"
TATA_signal 1413..1422
/gene="glucanase GLA"
exon 1448..1554
/number=1
/gene="glucanase GLA"
intron 1555..2343
/gene="glucanase GLA"
exon 2344..3820
/number=2
/gene="glucanase GLA"
mat_peptide 2349..3296
/EC_number="3.2.1.39"
/product="glucan beta-1,3-glucanase"
/gene="glucanase GLA"
/codon_start=2349
polyA_signal 3564..3569
/gene="glucanase GLA"
polyA_signal 3600..3605
/gene="glucanase GLA"
BASE COUNT 1418 a 837 c 744 g 1483 t
ORIGIN
1 gagctcctct agggggccaa ggcaaacctt tttgctaatg ggaaaaaaat attagaccaa
61 gagtttgtaa tagctactca atttcaattt acaaagggga aaatttaact gttttgccct
121 tatatctttt ggtcccgaaa cataaaatat cccatccgaa attccaaatg gtccattatc
181 ggcaagtagc tttcttttat tatagttagt tgacaaaaca ctatcgagat atcattagta
241 taataataac ttcaaagtcc atcttcttag ctgcctcctc attagagccg ccactaaaat
301 aagaccgatc aaataaaagc cgccattaaa ataatgaatt ttaggactct caattgtcac
361 gtaagtgcca aaactcttcc aatactttgc tgcaacttgg ggctgctagc ttctgagctt
421 cctgggatat ttctatgttt atctcttaat ttacatctca actaatatca agaaattaaa
481 caggtgcagc aaatcataaa attttcctct aaagaagaaa atgactccgg ttactgattc
541 attggccttt tcagagtctg cgtgccatat tcactaatgg gtcgtttggt acaagaaata
601 atgataataa tttcgaaata gaatttggga tttcatttat ttcatattta attataaata
661 ttagctaatt tcgaaataaa ttttacatta aaatagtgaa atcaactatc tcatatataa
721 ggtggaatag ctaatctcat agccacctca gttagaatcc agtttcctct aataaatgca
781 gcgaaatatt agaagacttt cattaaatca attcatataa tttaaaaata ctagacatgg
841 aaaaaaaaaa cgattcgaga ctgttatgga aggcgttgcc ttcgatgtag attctcatcc
901 attgctttcg tgcaatagca atatgacatc ttattcttag aactactttt aaatgaaagt
961 catatagaac tttaaaatct ctcaactagt tttaagggaa ttcaaaatac gaccaatatt
1021 tattacttac ttatggataa atttaaataa tatgtatttt atcttgaaat tgaattgaaa
1081 atattaaatt acttggttta atataaacaa tagatatcgt taagtattta ccacaaacat
1141 tttattagtt gtaacgatga ttaagcagga attcctctgg ttgtgcagga tgaaagaaac
1201 taactagcta taatttcttt tgtaaagtca agatagtacg gcaccttatg gagaaaataa
1261 ataactttac atcatcaaac tcatcttctt ttttccacaa aatgatcaag ttgacatgtt
1321 aatagccagg tcaccggggg cggctcttaa cttcattagc ctacgaataa caaatccaat
1381 attatattta cacaaggcta tatatatatc tcaatataaa tagctcgttg ttcatcttaa
1441 ttctcccaac aagtcttccc atcatgtcta cctcacataa acataatact cctcaaatgg
1501 ctgctatcac actcctagga ttactacttg ttgccagcag cattgacata gcaggtttct
1561 ggtcaaatat ttgaacttcc cagccaaaaa tattgtctta taattttgtg tgcgcaaaat
1621 tttaatttag ttgatagtta tttgcttatt tttcttttca aattgcttgt gtttttttct
1681 caaattaact tgcaccgtat tcatttagcg atagttattt gctctatttt tgtgtaacac
1741 tcactcacaa acttttcaat ttgaggggag gacagtgaat ctaagattga aatttatgag
1801 tttaattaga ctaattccca tttgatttat tggctagaag tcaattattt gcatagtgag
1861 tcttttaaca cacagatttg agttaaagct actacgttcg tattaaccca taacatatac
1921 accttctgtt ctaatttctt tgacactttt tgttagtttg ttccaaaaag gacggacata
1981 tttgatattt gagaatactt taccttaacc ttaatagaat tttttatgac atcacatata
2041 ttatggaata tatacgacca taattttcaa atatcttata gtcgtacaaa tattatagca
2101 tgtttaatac cacaactttc aaattcttct tttccttaaa aacaaaatat gtcacataaa
2161 ttaaaataga ggaagtatac tacatcaatc agcccctagt ggaggggacc ctactgtaag
2221 tttttaagtt ttcaagaatt cagtaattga ttaggagccc gtctggacat aaaaaaaaat
2281 tccttttttt ccaaaaaatg cccactaaat ttctaacact attttgtaat tcttattgag
2341 caggggctca atcgataggt gtttgctatg gaatgctagg caacaacttg ccaaatcatt
2401 gggaagttat acagctctac aagtcaagaa acataggaag actgaggctt tatgatccaa
2461 atcatggagc tttacaagca ttaaaaggct caaatattga agttatgtta ggacttccca
2521 attcagatgt gaagcacatt gcttccggaa tggaacatgc aagatggtgg gtacagaaaa
2581 atgttaaaga tttctggcca gatgttaaga ttaagtatat tgctgttggg aatgaaatca
2641 gccctgtcac tggcacatct tacctaacct catttcttac tcctgctatg gtaaatattt
2701 acaaagcaat tggtgaagct ggtttgggaa acaacatcaa ggtctcaact tctgtagaca
2761 tgaccttgat tggaaactct tatccaccat cacagggttc gtttaggaac gatgctaggt
2821 ggtttgttga tcccattgtt ggcttcttaa gggacacacg tgcaccttta ctcgttaaca
2881 tttaccccta tttcagttat tctggtaatc caggccagat ttctctcccc tattctcttt
2941 ttacagcacc aaatgtggtg gtacaagatg gttcccgcca atataggaac ttatttgatg
3001 caatgctgga ttctgtgtat gctgccctcg agcgatcagg aggggcatct gtagggattg
3061 ttgtgtccga gagtggctgg ccatctgctg gtgcatttgg agccacatat gacaatgcag
3121 caacttactt gaggaactta attcaacacg ctaaagaggg tagcccaaga aagcctggac
3181 ctattgagac ctatatattt gccatgtttg atgagaacaa caagaaccct gaactggaga
3241 aacattttgg attgttttcc cccaacaagc agcccaaata taatatcaac tttggggtct
3301 ctggtggagt ttgggacagt tcagttgaaa ctaatgctac tgcttctctc gtaagtgaga
3361 tgtgagctga tgagacactt gaaatctctt tacatacgta ttccttggat ggaaaaccta
3421 gtaaaaacaa gagaaatttt ttctttatgc aagatactaa ataacattgc atgtctctgt
3481 aagtcctcat ggattgttat ccagtgacga tgcaactctg agtggtttta aattcctttt
3541 ctttgtgata ttggtaattt ggcaagaaac tttctgtaag tttgtgaatt tcatgcatca
3601 ttaattatac atcagttcca tgtttgatca gattgggatt tggtaacttc aatgtttagt
3661 tatttattaa ttagtgtctt tatcatttga ctatcaatta atctttattt ggcaaggctt
3721 gatatatttg agttactctt aggtatttgc aagcaactga tctttctttt atcccgtttc
3781 tccgttaaac ctcattagaa atatattata atgtcaccta ctctgtggtt taagacattc
3841 ccttacatta taaggtattt cacgtcgtat caggtcgaaa aaaataatgg tacgctcttt
3901 cttatcacaa atttctctca acttctagac caattgaatc ttgtctccaa taagcattgc
3961 ttttactgta tgtttctctc tatcaattca aggctcaagc catcaaatcg cttggtattt
4021 ctcgctccca tttaaccaat cgagctgtta gctctgctaa agtctcattc ttcagcttct
4081 caaaccactc agagcttctc caaccaaaaa tgtagaaaaa atctttgatc cacatgtaaa
4141 accccagaat tgtcattcga gtgaaggaag gtgctggaaa cgcgacatca aacacggatt
4201 tgccagctga ttgtcatagg aaaaagaaaa gttgatcaga tagttcgtgc ggggtatttc
4261 ctacctcgcc tttgatcata gctctaggct tgcgaaacta agttatgcac gaggccccct
4321 gcgaatccat aaatctaaga agcatgccac aacaaacggg ataacttcca gctaagagat
4381 ctattttgat cttacccaca aactagctcc gtgtgaattg tccatttcat caactccaaa
4441 ggcaggaaag aagactctat tcagagagca gcaaaagagc tc
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