GenBank-Updates@genbank.bio.net (03/16/91)
LOCUS TOBGLB13B 4699 bp ds-DNA PLN 16-MAR-1991
DEFINITION Nicotiana tabacum glucan beta-1,3-glucosidase gene, complete cds.
ACCESSION M60403
KEYWORDS beta-1,3-glucanase; glucan beta-1,3-glucosidase.
SOURCE Tobacco (strain Havana 425) leaf DNA, clone lambda-GLB.
ORGANISM Nicotiana tabacum
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Asteridae; Solanales; Solanaceae.
REFERENCE 1 (bases 1 to 4699)
AUTHORS Sperisen,C., Ryals,J. and Meins,F.Jr..
TITLE Comparison of cloned genes provides evidence for intergenomic
exchange of DNA in the evolution of a tobacco beta-1,3-glucanase
gene family
JOURNAL Proc. Natl. Acad. Sci. U.S.A. (1991) In press
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS join(1648..1738,2486..3507)
/EC_number="3.2.1.39"
/product="glucan-1,3-beta-glucosidase"
/gene="glucanase GLB"
/codon_start=1648
sig_peptide join(1648..1738,2486..2490)
/gene="glucanase GLB"
/codon_start=1648
mRNA join(1632..1738,2486..3991)
/note="major transcription start site"
/product="glucan-1,3-beta-glucosidase"
/gene="glucanase GLB"
TATA_signal 1597..1606
/gene="glucanase GLB"
exon 1632..1738
/number=1
/gene="glucanase GLB"
intron 1739..2485
/gene="glucanase GLB"
exon 2486..3991
/number=2
/gene="glucanase GLB"
mat_peptide 2491..3438
/EC_number="3.2.1.39"
/product="glucan-1,3-beta-glucosidase"
/gene="glucanase GLB"
/codon_start=2491
polyA_signal 3703..3708
/gene="glucanase GLB"
BASE COUNT 1509 a 857 c 786 g 1547 t
ORIGIN
1 gagctccctt ggggggcaag ggcaaaactt tttgctaaat ggaaaaatat tataccaagt
61 gtttgtaata gttactcaat ttgaattaac aaaggggcaa atttgactat tttgccctta
121 tatcttttgg tcacaaaaac ataaaatatc ccatccgaaa ttccaaatgg tccattatcg
181 gcaagtagct ttctttaatt atagttagtt gacaaaacac tatcaagata tcattattat
241 aataataact tcaaagtcca tcatcttagc tgcctcctca gtagagccgc cagtaaaata
301 agaccgatca aataaaagcc gccattaaaa taatgaattt taggactctc gattggcacg
361 taagtgccaa aactcttcca atactttgct gcaacttggg gctgctaggt tctgagcttc
421 cagatatggg atatttctaa gtttatctcc taatttacat ctcaactaat attaagaaat
481 taaacaggta cagcaaatca taaaattttc ctctaaagaa gacaatgaat ccggttactg
541 attcattggc cttttcagag tctgcatgcc atattcacta aggggtcgtt tggtacaaga
601 aataataata ataatttcgg gatagaattt gagattgcat ttatcttgtg tttaattata
661 agtattagct aatttcagaa taaattttac actaaaatag taaaatcaac tatcacatgt
721 agaaggtgga atggaatagc taatcccata gccactcaca tagaatatcc ttatttatct
781 cactatttta ccaaatgatc ggttagtctt catgagaatc cagtatcctc aataaatgca
841 gtaagaagtt agaaaatttt cattaaatca attcatataa tttaaaaata ttagatatgg
901 agcacttaag atacaataaa agatgtaccg ttaataataa aagataagat agagttttaa
961 ataggaaaaa aaaaacggtt cgagacactc ttatggaagg cgttgtcttc aaagtagatt
1021 ctcattcatt gctctggtgc aatagcaaaa tgacatctta ctcttaagat acagcgagcc
1081 actctacaat cttctattgt atactcaaat gaaagtttta gagaactttc aaatctctca
1141 actactttta agggaattca aaatacgacc aatatttatt acttacttac ttatagttaa
1201 atgatatgaa ttttatttta aatttgaatt gaaaatatta aattacttga tttaatataa
1261 acaatagata tcgctaagta tttaccacaa acatggagat actacagaag attttattat
1321 ttgtaacgat gattaagcag ctattcatct ggttgtgcag gatgaaagaa agtaactagc
1381 tataatttct tttgtaaagt caagatagta cggcaccttt ggagaaaata taaaacttgt
1441 acatcatcaa actcattttc ttttttccac aaaatgatca agttgacatg ttaatagcca
1501 ggtcagccgg ggcggctctt aacttcatta gcctacgaat aacaaatact ccaataatat
1561 tccactcgaa tatttacatt tacacaaggc tatctcaata taaatagctc attgttcatc
1621 ttaattcttc caacaagtct tctcatcatg tctacctcag ataaacataa tactcctcaa
1681 atggctgcta tcacactgct aggattacta cttgttgcca gcaccattga gatagcaggt
1741 ttctggtcaa atatttgaac ttccgagcca aaaatattgt cttataattt tgttggtgca
1801 aaatcttaat ttagttgata gttatttgct tatttttctt atcaaattgc ttgtgttttt
1861 ttctcaaatt tacttgcact atattcattt agcgatagtt atttgctctt ttttcgggta
1921 acactcactc acaagctttt caaatttgag gggagggcgg tgaatctaaa atttgaaatt
1981 tatgagttta gactagtgtc catttgattt attggctaga cgtctattag ttgtatagta
2041 aatcttttaa cacatacacc gacctgagtc aaagctatta ggttcgtatt aacacataac
2101 acatattccc tctgttctaa tttatgtgac acactttctg ttagtttctt ccaaagagaa
2161 tgacatattt gatatttaaa aatattttaa ctttaaactt tttattctca accttttata
2221 acatcacaaa tattatggaa catattagac tacaagttcc aaatatctta tagtctgtac
2281 aaatattata gcatgtttaa taacacaatt ttcacattct tctttttctt aaactttgtg
2341 ccgaattaaa ttatgtcaca taaattaaaa tggttacatc atcccctagt ggagggacct
2401 accatgtcta ctgtaagttt ttaacttttc aagaattaca taattgattt agtttctaac
2461 actaattcta attcttattg agcaggggct caatcaatag gtgtttgcta tggaatgcta
2521 ggcaacaact tgccaaatca ttgggaagtt atacagctct acaagtcaag aaacatagga
2581 agactgaggc tttatgatcc aaatcatgga gctttacaag cattaaaagg ctcaaacatt
2641 gaagttatgt taggacttcc caattcagat gtcaagcaca ttgcttccgg aatggaacat
2701 gcaagatggt gggtacaaaa aaatgttaaa gatttctggc cagatgttaa gattaagtat
2761 attgctgttg ggaatgaaat cagccctgtc acaggcacat cttaccttac ctcatttctt
2821 actcctgcca tggtaaatat ttacaaagca attggtgaag ctggtttagg aaacaacatc
2881 aaggtctcaa cttctgtaga catgaccttg attggaaact cttatccacc atcacagggt
2941 tcgtttagga acgatgctag gtggtttact gatccaattg ttgggttctt aagggacaca
3001 cgtgcacctt tactcgttaa catttacccc tatttcagct attctggtaa tccagggcag
3061 atttctctcc cctattctct ttttacagca ccaaatgtgg tagtacaaga tggttcacgc
3121 caatatagga acttatttga tgcaatgctg gattctgtgt atgctgccct cgagcgatca
3181 ggaggggcat ctgtagggat tgttgtgtcc gagagtggct ggccatctgc tggtgcattt
3241 ggagctacat atgacaatgc agcaacttac ttgaggaact taattcaaca cgctaaagag
3301 ggtagcccaa gaaagcctgg acctattgag acctatatat ttgccatgtt tgatgagaac
3361 aacaagaacc ctgaactgga gaaacatttt ggattgtttt cccccaacaa gcagcccaaa
3421 tataatctca actttggggt ctctggtggt gtttgggaca gttcagttga aactaatgct
3481 actgcttctc tcataagtga gatgtgagat gagacacttg aaatctcttt acataagtat
3541 tgcttagatg gaaagcttag taaaaacaag agaaatttat tcttcatgca agacactaaa
3601 taacattgca cgtctctgta agtcctcatg gattgttatc cagagacgat gcaactctga
3661 gtggttttaa atcccttttc tttgtgatgt tggtaatttg gcaagaaact ttctgtaagt
3721 ttgtgaattt catgactttc tgtaagtttg tgaatttcat gcaccatcaa ttatacatct
3781 tttccatgtt tgatcacatt aggatttggt aattgcaaag tttagttatt tattaattag
3841 tgtctttatc atttgactcg atcaattaat ctttaattgg taaggcttga tatatcggag
3901 cgactcttag gtagtggcat tcaactgatc tttctgttat cccatgtctc cgttaaccct
3961 cattagaaat atattataat gtcacctaaa tcagaggttt tagagcttca aaatcgattg
4021 acaacagttt tggagttaca ctgtggttta ggacattctg ttacattata aggtatttca
4081 cgtcgtatca aggtcgaata aaataatggt acgctctttc ttatcacaaa tttctctcaa
4141 cttctagacc aattgaatct tgtctccaat aagtattgct tttactctat gtttctctct
4201 atcaattcaa ggctcaagcc atcaaatcgc ttggtatttc tcgctctcaa ttaaccaatc
4261 gagctgttaa ctctgctaaa gtctcattct tcagcttctc aaaccactca aagcttctcc
4321 aacaaaaaaa gtagaaaaaa actttgatcc acatgtaaaa ccccataacc atgtcattcg
4381 agtgaaggaa ggtgcttgaa atgcaacgaa caaacacggc tttgccactg cttgtgatag
4441 gtaaaagaaa acttgattag atagttcgtg cggggcattt cctacctcgc ctttgatctt
4501 agctttaggc ttgcgaaact aagtagaacc taaggcccca gcgaatccgt aaatctgagg
4561 agcatgccac agcaaaaagg ttagctttca gataagagat ctatttgatc ttacccacta
4621 actagctctg tgtgaattgt ccatttcatc aactccaaag gcaggaaaga agactattga
4681 gagagcagca aaagagctc
//