GenBank-Updates@genbank.bio.net (03/15/91)
LOCUS SYNAADA 2082 bp ds-DNA SYN 15-MAR-1991
DEFINITION R100.1 plasmid/bacteriophage T4/synthetic aminoglycoside
3/adenyltransferase Aad fusion protein gene, complete cds.
ACCESSION M60473
KEYWORDS aminoglycoside 3/adenyltransferase Aad fusion protein.
SOURCE R100.1 plasmid/bacteriophage T4/synthetic recombinant DNA.
ORGANISM Artificial gene
Artificial sequences; Genes.
REFERENCE 1 (bases 1 to 2082)
AUTHORS Prentki,P., Binda,A. and Epstein,A.
TITLE Plasmid vectors to select IS1-promoted deletions in cloned DNA and
their use in the nucleotide sequence analysis of the omega fragment
JOURNAL Unpublished (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 884..1675
/function="resistance to streptomycin/spectinomycin"
/product="aminoglycoside 3/adenyltransferase Aad fusion
protein"
/gene="aadA"
/evidence=EXPERIMENTAL
/codon_start=884
BASE COUNT 533 a 525 c 536 g 488 t
ORIGIN
1 gaattcccgg ggatccggtg attgattgag caagctttat gcttgtaaac cgttttgtga
61 aaaaattttt aaaataaaaa aggggacctc tagggtcccc aattaattag taatataatc
121 tattaaaggt cattcaaaag gtcatccacc ggatcaattc ccctgctcgc gcaggctggg
181 tgccaagctc tcgggtaaca tcaaggcccg atccttggag cccttgccct cccgcacgat
241 gatcgtgccg tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct cactgatccg
301 catgcccgtt ccatacagaa gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag
361 gatgcgaacc acttcatccg gggtcagcac caccggcaag cgccgcgacg gccgaggtct
421 tccgatctcc tgaagccagg gcagatccgt gcacagcacc ttgccgtaga agaacagcaa
481 ggccgccaat gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga
541 cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg
601 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
661 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
721 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
781 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
841 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat
901 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
961 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
1021 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
1081 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
1141 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
1201 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
1261 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
1321 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
1381 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
1441 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
1501 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
1561 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
1621 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
1681 aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac
1741 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
1801 cgtgcataat aagccctaca caaattggga gatatatcat gaaaggctgg ctttttcttg
1861 ttatcgcaat agttggcgaa gtaatcgcaa catccgcatt aaaatctagc gagggcttta
1921 ctaagctgat ccggtggatg accttttgaa tgacctttaa tagattatat tactaattaa
1981 ttggggaccc tagaggtccc cttttttatt ttaaaaattt tttcacaaaa cggtttacaa
2041 gcataaagct tgctcaatca atcaccggat ccccgggaat tc
//GenBank-Updates@genbank.bio.net (03/16/91)
LOCUS SYNAADA 2082 bp ds-DNA SYN 16-MAR-1991
DEFINITION R100.1 plasmid/bacteriophage T4/synthetic aminoglycoside
3/adenyltransferase Aad fusion protein gene, complete cds.
ACCESSION M60473
KEYWORDS Omega fragment;
aminoglycoside 3/adenyltransferase Aad fusion protein.
SOURCE R100.1 plasmid/bacteriophage T4/synthetic recombinant DNA.
ORGANISM Artificial gene
Artificial sequences; Genes.
REFERENCE 1 (bases 1 to 2082)
AUTHORS Prentki,P., Binda,A. and Epstein,A.
TITLE Plasmid vectors to select IS1-promoted deletions in cloned DNA and
their use in the nucleotide sequence analysis of the omega fragment
JOURNAL Unpublished (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 884..1675
/function="resistance to streptomycin/spectinomycin"
/product="aminoglycoside 3/adenyltransferase Aad fusion
protein"
/gene="aadA"
/evidence=EXPERIMENTAL
/codon_start=884
BASE COUNT 533 a 525 c 536 g 488 t
ORIGIN
1 gaattcccgg ggatccggtg attgattgag caagctttat gcttgtaaac cgttttgtga
61 aaaaattttt aaaataaaaa aggggacctc tagggtcccc aattaattag taatataatc
121 tattaaaggt cattcaaaag gtcatccacc ggatcaattc ccctgctcgc gcaggctggg
181 tgccaagctc tcgggtaaca tcaaggcccg atccttggag cccttgccct cccgcacgat
241 gatcgtgccg tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct cactgatccg
301 catgcccgtt ccatacagaa gctgggcgaa caaacgatgc tcgccttcca gaaaaccgag
361 gatgcgaacc acttcatccg gggtcagcac caccggcaag cgccgcgacg gccgaggtct
421 tccgatctcc tgaagccagg gcagatccgt gcacagcacc ttgccgtaga agaacagcaa
481 ggccgccaat gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg ccagccagga
541 cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac cgtggaaacg
601 gatgaaggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta
661 gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc
721 agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc
781 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
841 tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatgaggg aagcggtgat
901 cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc
961 gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag
1021 tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt
1081 gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt
1141 agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga
1201 actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac
1261 gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt
1321 aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct
1381 aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt
1441 agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga
1501 tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga
1561 agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt
1621 ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct
1681 aacaattcgt tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac
1741 taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag
1801 cgtgcataat aagccctaca caaattggga gatatatcat gaaaggctgg ctttttcttg
1861 ttatcgcaat agttggcgaa gtaatcgcaa catccgcatt aaaatctagc gagggcttta
1921 ctaagctgat ccggtggatg accttttgaa tgacctttaa tagattatat tactaattaa
1981 ttggggaccc tagaggtccc cttttttatt ttaaaaattt tttcacaaaa cggtttacaa
2041 gcataaagct tgctcaatca atcaccggat ccccgggaat tc
//