GenBank-Updates@genbank.bio.net (03/19/91)
LOCUS TRHTCSA 1010 bp ss-mRNA PLN 19-MAR-1991
DEFINITION T.kirilowii trichosanthin (TCS) mRNA, complete cds.
ACCESSION M34858
KEYWORDS ribosome inactivating protein; trichosanthin.
SOURCE T.kirilowii maximowicz, cDNA to mRNA.
ORGANISM Trichosanthes kirilowii
Eukaryota; Plantae; Embryobionta; Magnoliophyta; Magnoliopsida;
Dilleniidae; Violales; Cucurbitaceae.
REFERENCE 1 (bases 1 to 1010)
AUTHORS Shaw,P.-C., Yung,M.-H., Zhu,R.-H., Ho,W.K.-K., Ng,T.-B. and
Yeung,H.-W.
TITLE Cloning of trichosanthin cDNA and its expression in Escherichia
coli
JOURNAL Gene 97, 267-272 (1991)
STANDARD simple staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by P.-C.Shaw, 06-JUN-1990.
Author address: P.-C.Shaw
Department of Biochemistry
Chinese University of Hong Kong
Shatin, NT, HONG KONG
FEATURES Location/Qualifiers
CDS 10..879
/note="trichosanthin precursor"
/codon_start=10
sig_peptide 10..78
/note="trichosanthin signal peptide"
/codon_start=10
mat_peptide 79..819
/note="trichosanthin"
/codon_start=79
variation 196..196
/note="t in wild type; a in allele"
variation 197..197
/note="c in wild type; g in allele"
variation 468..468
/note="t in wild type; a in allele"
BASE COUNT 290 a 218 c 205 g 297 t
ORIGIN
1 gtcaaaaaga tgatcagatt cttagtcctc tctttgctaa ttctcaccct cttcctaaca
61 actcctgctg tggagggcga tgttagcttc cgtttatcag gtgcaacaag cagttcctat
121 ggagttttca tttcaaatct gagaaaagct cttccaaatg aaaggaaact gtacgatatc
181 cctctgttac gttcctctct tccaggttct caacgctacg cattgatcca tctcacaaat
241 tacgccgatg aaaccatttc agtggccata gacgtaacga acgtctatat tatgggatat
301 cgcgctggcg atacatccta ttttttcaac gaggcttctg caacagaagc tgcaaaatat
361 gtattcaaag acgctatgcg aaaagttacg cttccatatt ctggcaatta cgaaaggctt
421 caaactgctg caggcaaaat aagggaaaat attccgcttg gactccctgc tttggacagt
481 gccattacca ctttgtttta ctacaacgcc aattctgctg cgtcggcact tatggtactc
541 attcagtcga cgtctgaggc tgcgaggtat aaatttattg agcaacaaat tgggaagcgt
601 gttgacaaaa ccttcctacc aagtttagca attataagtt tggaaaatag ttggtctgct
661 ctctccaagc aaattcagat agcgagtact aataatggac agtttgaaag tcctgttgtg
721 cttataaatg ctcaaaacca acgagtcacg ataaccaatg ttgatgctgg agttgtaacc
781 tccaacatcg cgttgctgct gaatagaaac aatatggcag ccatggatga cgatgttcct
841 atgacacaga gctttggatg tggaagttat gctatttagt gtaacttcaa gctacgtacg
901 agtacaaact cccacttgaa gaatctatta tcgtttgaga gtttaatcta cttgtagaaa
961 taataaagca tgttcgtgtg accgacctac gtggatgctc tgtatgtgtg
//