GenBank-Updates@genbank.bio.net (03/19/91)
LOCUS NEUPHO4A 4572 bp ds-DNA PLN 19-MAR-1991
DEFINITION N.crassa phosphate-repressible phosphate permease gene, complete
cds.
ACCESSION M31364
KEYWORDS phosphate permease.
SOURCE N.crassa (strain RLM57035) spheroplast DNA.
ORGANISM Neurospora crassa
Eukaryota; Plantae; Thallobionta; Eumycota; Ascomycotina;
Pyrenomycetes; Sordariales; Sordariaceae.
REFERENCE 1 (bases 1 to 4572)
AUTHORS Mann,B.J., Bowman,B.J., Grotelueschen,J. and Metzenberg,R.L.
TITLE Nucleotide sequence of pho-4+, encoding a phosphate-repressible
phosphate permease of Neurospora crassa
JOURNAL Gene 83, 281-289 (1989)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS join(1652..1724,1805..3301,3385..3587)
/note="phosphate permease"
/codon_start=1652
misc_feature 583..623
/note="region of dyad symmetry"
prim_transcript 614..3820
/note="pho4 mRNA and introns (alt.)"
misc_feature 650..683
/note="region of dyad symmetry"
prim_transcript 1506..3820
/note="pho4 mRNA and introns (alt.)"
exon 1652..1724
/note="phosphate permease, exon 1"
intron 1725..1804
/note="pho4 intron A"
exon 1805..3301
/note="phosphate permease, exon 2"
intron 3302..3384
/note="pho4 intron B"
exon 3385..3587
/note="phosphate permease, exon 3"
BASE COUNT 953 a 1353 c 1118 g 1148 t
ORIGIN 80 bp upstream of KpnI site.
1 tcttcaacca cgtggactct tccaggcgtc ctattcgcca aaaaaggcat caagattgtg
61 gcgtttcggg aggcaggtac caatggcgta tatcttggct ttcttcttgg cgctttgctt
121 cttggcatct ctttcttcaa cgccgggagt tcgagtcact gacccacgtg tgtcgacggc
181 gagcgttgat ttcttcattc tcgacatgcc aacgccactc tgccgaccca ttggcaatat
241 ataagcattc agctcttcgc ccttggcttt tcttggctgc ttcggccaag acctcttctt
301 ttactcttgt tattgtcgtt ggacctttct tcctcggggt agttcctctt ctggcccaag
361 ccaacccgca ttggagatat gggatggacg tgttgtatgt tggacgaggt gatttgctgg
421 aatcactact tttatgggat gatctcgagc ttggagagtg tttgaccatg gcggacttgt
481 tcaatcacgg cgggactatg ttcgttatgt gtggatatcg acttctgtct ggttcatcca
541 gaagctctga aagtgggaga cactcgagga gaactcaacg ccgagctcaa gaaacttgac
601 cgagtggtca atatcttgag cttcaaggcc agatatacta cggtggatat acctgcagtc
661 ttccaatggg gcagacctca ggtgacacgt ctgcgaggaa gccccccaag cgttcaaaac
721 gagacaagca gtaagtatca ttgtccaatc ttctttccaa acccctttcg gctgttcaca
781 ggaattgcac tcactgccac atcactgtca cgcgccttca gccctcaatg attcaagggt
841 ttccatgcaa tcatgttcgt tgcagaagcg aagcacgttc accatcccac gcttcttgac
901 gtccaatgtt acttccagcc ttctctggag gagtcgatcg agatgttggt gggtgaacgg
961 tacctgacct accaatcctt ctcttcctat tccttttccc ctggactcca cttccccaag
1021 ggttcacggt cttgtcatta cggtcttgtt cgacaaaaga tcactagcat gatatgcact
1081 cgccacgcgc cgagcacgcc cagaaccgaa aacgatgaag attcctccac cacggcgggc
1141 atgcccagaa gcagttaaca acagcgttcg taccaccacc acggccacca ccactattct
1201 cacaccatct cgttcacctc acctctttgc cacaccgtta atgtgtgtgg ggcaagcgaa
1261 tatgcagcag ttaggtcctt ggctggcgaa caccaagaaa cattcacatc ctgagatacc
1321 acacacgtgt gcccgaccct tggatgggat cgcctcccat acgcattccg ttggttttcg
1381 acgggacgta cctgttgcaa ccttgctgca gtactctatg cacgaacaat ccatcccgcg
1441 cagtcttgta tatcctacat ataggaccca accatggaaa ccatccgagc ccaatcctgt
1501 cttctcttca gcaccaagcc gctcaccaaa caaaagaaaa caaccagagc aagctcaaga
1561 gcttctctta gagacgctcg cgtgccttcc ctcacgcgac caactaccct cttttcctca
1621 ttgttttggt tgaagcaaca tatcgttcaa gatggttctc catcagttcg attatctcct
1681 tgccatcggc accatctttg ctgcccttga tgcttggaac attggtatgt caaataacac
1741 cacctggccc atattcaacc aagcccttgc aacattcact gacaaccatt cccttccaca
1801 acaggagcca acgatgttgc caactcctgg gccacctcgg tcgccgcccg ctccgtcacc
1861 tacctccaag ccatgatcct tggctccatc atggaattcg ccggttctgt tggcgtgggt
1921 gcccgcgtag ccgacaccat ccgcactaaa gtcgtcgaca ccactctctt tgccgatgac
1981 ccggccctcc tgatgctggg catggtctgc gccgtcgttg cctcctccat ctacctcacc
2041 atggccaccc gcttcggcct acccgtctct accacacact ccatcatggg tggtgtcatc
2101 ggcatgggca tcgcggctgt cggtgccgac ggcgtccaat gggtcggctc gagcatcaac
2161 gatggcgtgg tatcggtctt cctggcctgg gtcatcgcgc ccggcctcgc cggcgccttc
2221 gcgtccatca tcttcctcgt caccaagtac ggcgtgctgc tccgttcgaa cccggtctac
2281 aaggctttcg ttatggtgcc catctatttt ggtatcaccg cggcgctgct ctgcatgctc
2341 ctgctgtgga agggcggcag ctacaaggtg acgttaacca acccggagat tgcgggcacc
2401 atcatcggtg tcggcgccgc ctgggccttg ctagtcacca tctttttgat gccttggctt
2461 taccgcatcg tcatcctcga ggactggcag ctccgtttct ggcacatccc tctcggcccc
2521 ctccttctcc gtcgcggaga ggtgcctccc ccgcctgcgg acggctccgg tgtcgtccag
2581 gatttctatg ccggtcgctt gacaaaggag caactcgccg cccgccgcgc cgctcaaaac
2641 ggtgactcgg aaatggccgc cggtgccgtc acatcctcca cctccaaccc ctcggctccc
2701 accgacggcg aaaagggggc caccatcacc aaggacgact cctcctactc gcacgaccat
2761 tctgagcccg cccaagccgc ccaaccccaa atcaagacca tggtcggccc tcgccctgct
2821 ggtccctggc actcgggcgc cgttctcttc tggtacgtca agtgggcctt gttccgcggc
2881 gtcgaccaag acgtcctctc ctcgcagcaa gaaaaatccg tcatctcgtc cgacgttgaa
2941 gaactccacg cgcacgcgac gcactacgac aacaagaccg agtacatgta ctcctttttg
3001 caaatcatga cggcagccgc cgcctctttc acgcacggcg ccaacgacat tgccaacgcg
3061 attggccctt atgcgactgt tttccagctc tggaaggacg gcgcgcttcc cgagaagggc
3121 aaggcggatg ttcccgtgtg gattttggtg tttggtgctt cctgcttggt gattggcctg
3181 tggacgtatg ggtataacat catgaggaac ttgggcaacc ggattacgtt gcagagtccg
3241 agcagagggt tttcgatgga gttggggagc gcggtgacgg ttattttggc tactcggttg
3301 agtaagtatt tgtttttctt tcgtttgatt cgagactgcc gatgcatggg gcgaacttgc
3361 ttgctaacga cgattgactt acagagctgc ctgtgtcgac tacgcagtgt attacgggcg
3421 ctactgtcgg tgttggtctt tgctcgggca cgtggaggac catcaactgg cgccttgtgg
3481 cttggatcta catgggctgg ttcatcactc tgcctgttgc aggcatcatt tcgggatgtt
3541 tgatgggtat cattatcaat gctcctcgct gggggtatag tggctgaatg cacgctgtgg
3601 ggtggatgtc ggaatagagg tgccgctcga cagaaaaaag aggagtgggt gtgaccaagg
3661 atggatggaa aagaattgga actgtccaga atttgtgttc tattccttca aaccacgtcg
3721 ccgcaggtgg ttacgtagta gatagctgtt gcttgttttg tgctattttg tgaggctgtt
3781 ataccccagg tagtacgcta ttctgaaatc catatcaacc aaacgctaat tgagaacatg
3841 aacatggcaa gctcctagtg ctcttcatca ccaatttctg ttccttgcca gctttcaatc
3901 atcgatgtaa tcataatctt aaagcaggag ctccggagtg aaggccaccc gtagacgctt
3961 gggcgtgctc tcatcggatg ggggagtgct gtcatcatag cgcttcgagc caagcctggg
4021 aggcaaggat ttgatccaca aaaccctttc catacccggc tgtatctcgc tcaacttgct
4081 gttcagacaa tggatttcag ccataatgcg tggacacggg caccttcgtt ggaagcccaa
4141 caaatttact cgttgggagt cattcttttt caagaacgtc tcctccggcc tccatggtct
4201 cctgaagccc atgcgaccag ataacgaagc ctctctccac aagtgtgctc agcctgttca
4261 gaagctggct tccgacgccc attcgacgca cagaagcatg gacatggatc acgatgtaaa
4321 gaacgtcgtc acaacgctct tcttggtccc agtcgtcctt caggatgcca attgcaaaac
4381 caacgatcca gtcttctcca gttgcgagct tggcgacggc aacccatagc atcccttttc
4441 taaggcaaag attcaagagg tctcccttat atgtcgtatg cctggcgcgc cacggcagcc
4501 atgttctcca tctcacggag gtcctcaagt tcagggtaga ggcaatttgg accttcttca
4561 agatgaggaa ga
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