GenBank-Updates@genbank.bio.net (02/23/91)
LOCUS ECORNPBW 4434 bp ds-DNA BCT 23-FEB-1991
DEFINITION E.coli rnpB gene and ORFs.
ACCESSION D90212
KEYWORDS M1-RNA; RNA component of RNaseP; rnpB gene.
SOURCE E.coli (strain K12; isolate W3110)genomic DNA, clone 6B5(#515) from
Kohara's Library.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 3056 to 3818)
AUTHORS Reed,R.E. and Altman,S.
TITLE Repeated sequences and open reading frames in the 3' flanking
region of the gene for the RNA subunit of Escherichia coli
ribonuclease P
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 80, 5359-5363 (1983)
STANDARD simple staff_entry
REFERENCE 2 (sites)
AUTHORS Schweizer,H. and Datta,P.
TITLE The complete nucleotide sequence of the tdc region of Escherichia
coli
JOURNAL Nucleic Acids Res. 17, 3994-3994 (1989)
STANDARD full staff_review
REFERENCE 3 (bases 1 to 4434)
AUTHORS Komine,Y. and Inokuchi,H.
JOURNAL Unpublished (1990)
STANDARD full staff_entry
COMMENT
[Nucleic Acids Res. 17, 3994-3994 (1989)] sites.
These data kindly submitted in computer readable form by:
Yuriko Komine
Department of Biophyics
Faculty of Science, Kyoto University
Sakyo-ku, Kyoto 606
Japan
Phone: 075-753-4200
Fax: 075-791-0271
FEATURES Location/Qualifiers
CDS <1..507
/note="ORF1 (AA at 1)"
CDS 528..1427
/note="ORF2"
CDS 1443..2669
/note="ORF3"
misc_RNA 2702..3078
/note="mature M1-RNA"
variation 3201..3201
/note="a in [J. Bacteriol. (1990) In press]; deletion in
[Proc. Natl. Acad. Sci. U.S.A. 80, 5359-5363 (1983)]"
variation 3278..3278
/note="a in [J. Bacteriol. (1990) In press]; deletion in
[Proc. Natl. Acad. Sci. U.S.A. 80, 5359-5363 (1983)]"
variation 3313..3313
/note="t in [J. Bacteriol. (1990) In press]; ta in [Proc.
Natl. Acad. Sci. U.S.A. 80, 5359-5363 (1983)]"
variation 3332..3332
/note="g in [J. Bacteriol. (1990) In press]; gt in [Proc.
Natl. Acad. Sci. U.S.A. 80, 5359-5363 (1983)]"
variation 3661..3661
/note="a in [J. Bacteriol. (1990) In press]; deletion in
[Proc. Natl. Acad. Sci. U.S.A. 80, 5359-5363 (1983)]"
CDS complement(3691..>4434)
/note="ORFX"
BASE COUNT 1130 a 980 c 1210 g 1114 t
ORIGIN 68 min on K12 map.
1 gatatcggtt tctataactt cctgattcct tttgtagaaa caaaagagga agcagagctg
61 gcggtggcat caacccgtta cccaccggaa ggcattcgcg gcgtctccgt ttctcaccgc
121 gccaatatgt ttggcaccgt ggcggattat ttcgctcagt cgaacaagaa catcactatt
181 ctggtccaga tagaaagtca gcagggcgta gataacgtcg atgccattgc cgctaccgaa
241 ggcgtagacg gcatcttcgt cggccccagc gatctggccg cggcattagg ccatctcggc
301 aatgcatcac acccggatgt acaaaaagca attcagcaca tttttaaccg tgccagcgcg
361 cacggcaaac ccagcggtat cctcgcgccg gtcgaagccg atgcgcgtcg ttatctggaa
421 tggggcgcga cgtttgtggc tgtcggcagc gatctcggcg tcttccgctc tgccactcag
481 aaactggctg atacctttaa aaaataacca ccaccgcaac aagagagatg attgatatga
541 ctatgaaagt tggttttatt ggcctgggga ttatgggtaa accaatgagt aaaaaccttc
601 tgaaagcagg ttactcgctg gtggttgctg accgtaaccc agaagctatt gctgacgtga
661 ttgctgcagg tgcagaaaca gcgtctacgg ctaaagcgat cgctgaacag tgcgacgtca
721 tcataaccat gctgccaaac tcccctcatg tgaaagaggt ggcgctgggt gagaatggca
781 ttattgaagg cgcgaagcca ggtacggtat tgatcgatat gagttctatc gcaccgctgg
841 caagccgtga aatcagcgaa gcgctgaaag cgaaaggcat tgatatgctg gatgctccgg
901 tgagcggcgg tgaaccgaaa gccatcgacg gtacgctgtc agtgatggtg ggcggcgaca
961 aggctatttt cgacaaatac tatgatttga tgaaagcgat ggcgggttcc gtggtgcata
1021 ccggggaaat cggtgcaggt aacgtcacca aactggcaaa tcaggtcatt gtggcgctga
1081 atattgccgc gatgtcagaa gcgttaacgc tggcaactaa agcgggcgtt aacccggacc
1141 tggtttatca ggcaattcgc ggtggactgg cgggcagtac cgtgctggat gccaaagcgc
1201 cgatggtgat ggaccgcaac ttcaagccgg gcttccgtat tgatctgcat attaaggatc
1261 tggcgaatgc gctggatact tctcacggcg tcggcgcaca actgccgctc acagctgcgg
1321 ttatggagat gatgcaggca ctgcgagcag atggtttagg aacggcggat catagcgccc
1381 tggcgtgcta ctacgaaaaa ctggcgaaag tcgaagttac tcgttaatga cgtcgtgccc
1441 ggatggcgta ttgcaatccg ggcctggaat ccaggccgaa taagagaaac gccctccggc
1501 gtcatgtggt aacaggcata ggtatgaaaa tcgtaatcgc cccagactct tataaagaaa
1561 gtttatctgc cagcgaggtt gcgcaggcga tagaaaaagg atttcgggaa atttttcctg
1621 atgcacagta cgtttctgtt ccggttgccg acggtggcga aggaacggtg gaagcgatga
1681 ttgcagccac ccagggggct gaacgtcacg cctgggttac agggccgctg ggcgagaaag
1741 tgaatgccag ttgggggatc tccggcgatg gcaaaaccgc gtttattgaa atggcggcgg
1801 ccagtgggct ggagctggta cctgcggaaa aacgcgatcc actcgtgacc acttcacgcg
1861 gcacaggcga gttaatcctg caggcgctgg agagcggtgc gacaaacatt attatcggca
1921 ttggcggcag cgctacaaat gatggcggcg caggcatggt acaggcgctg ggggcgaaat
1981 tatgcgacgc caacggcaat gaaattggtt ttggcggcgg tagtcttaat actctgaatg
2041 atattgatat ttccggcctc gatccgcgct taaaagattg cgtcattcgc gtcgcttgtg
2101 atgtcaccaa tccgctggtg ggcgataacg gcgcatcgcg catctttggc ccacaaaagg
2161 gagccagtga agcgatgatt gttgagctgg acaataacct ctctcactat gccgaggtca
2221 ttaaaaaagc gctgcatgtt gatgtgaaag atgtccccgg tgcaggagct gcgggtggta
2281 tgggcgcggc gctaatggcg tttcttggtg cggaactgaa aagtggtatt gaaatcgtca
2341 ctacggcgct gaatctggag gaacatattc acgattgtac gctggtgatc accggtgaag
2401 ggcgtattga cagccagagt attcacggga aggtaccgat tggtgtcgca aacgtggcga
2461 agaagtacca taaaccggtg attggcattg cgggtagcct gaccgatgat gttggcgttg
2521 tacatcagca tggcattgat gcggtcttca gcgtattgac cagcataggt acgttggacg
2581 aagcattccg cggggcttat gacaatatct gccgtgcttc acgtaatatc gccgcgacac
2641 tggcgattgg aatgcgcaac gcggggtgac aagggcgcgc aaaccctcta tactgcgcgc
2701 cgaagctgac cagacagtcg ccgcttcgtc gtcgtcctct tcgggggaga cgggcggagg
2761 ggaggaaagt ccgggctcca tagggcaggg tgccaggtaa cgcctggggg ggaaacccac
2821 gaccagtgca acagagagca aaccgccgat ggcccgcgca agcgggatca ggtaagggtg
2881 aaagggtgcg gtaagagcgc accgcgcggc tggtaacagt ccgtggcacg gtaaactcca
2941 cccggagcaa ggccaaatag gggttcataa ggtacggccc gtactgaacc cgggtaggct
3001 gcttgagcca gtgagcgatt gctggcctag atgaatgact gtccacgaca gaacccggct
3061 tatcggtcag tttcacctga tttacgtaaa aacccgcttc ggcgggtttt tgcttttgga
3121 ggggcagaaa gatgaatgac tgtccacgac gctataccca aaagaaagcg gcttatcggt
3181 cagtttcacc tggtttacgt aaaaacccgc ttcggcgggt ttttgctttt ggaggggcag
3241 aaagatgaat gactgtccac gacactatac ccaaaagaaa gcggcttatc ggtcagtttc
3301 acctgtttta cgtaaaaacc cgcttcggcg ggttttactt ttggaggggc agaaagatga
3361 atgactgtcc acgacactat acccaaaaga aagcggctta tcggtcagtt ttacctgatg
3421 tacgtaataa accgttccgg cgggtttcag attgttgagt gcgctttatt catgccggat
3481 gcggcgtgaa cgccttatcc tgcctacaaa agcacgcaaa ttcaatatat tgcagagatg
3541 gcgtaggcct gataagcgta gcgcatcagg catttttgcg gttgccatca gtttcaaacc
3601 cgcgtcagtg agtttttgct tttggtgcac agggtaagat gaataaatat ctacggtatt
3661 aataatagac tttgtaagaa gttattaagt ctatattgca tttaaaaggt cgtaatcata
3721 cgtatagtcc tctctggtaa aatattgctt ctctgagtta tatgcttcat atgtgttata
3781 ttccccgacc tcaaagccat tgtccggaga attttcgtct ttctcgaggg tattatctga
3841 aagtaactct tctgagctag agtcttctat gtttaaatcc atagtataat taatttgggg
3901 gacgaccaga taagcatttt ctttggtatt ttcataatct tcaatgatta tatcgatgta
3961 atcatatatt ttttctgatg agaattcttt catctctgtt aatttaattc tgttcaagca
4021 attgatgatt ttcgatgagc ttttatatat ttcttttgtt aattcatttt ctaatttatc
4081 tgtaagagtt gcccttggta tagattttat aattgcaaat ctcataattt ttaattgttc
4141 ttcggtaatg tctccaggct catttaaaca actttcgaca aacttgattt tttcttcaga
4201 atacatatcg ctaatgctta ttaaaacagg cgttattttt agtaaataat cccaggagat
4261 tgtggcgttt tgtgttgaac taacaaaatt aacctgaaac atgttggcat tagtaaaatt
4321 tgttgaattc aatttaaaag gatttaaaat agtattagcc atatgtttat ctgtgaaatc
4381 ataataatct aaatcgacat tacgtaagtc cagagaggat agatcaacga tatc
//GenBank-Updates@genbank.bio.net (03/19/91)
LOCUS ECORNPBW 4434 bp ds-DNA BCT 19-MAR-1991
DEFINITION E.coli rnpB gene and ORFs.
ACCESSION D90212
KEYWORDS M1-RNA; RNA component of RNaseP; rnpB gene.
SOURCE E.coli (strain K12; isolate W3110)genomic DNA, clone 6B5(#515) from
Kohara's Library.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 3056 to 3818)
AUTHORS Reed,R.E. and Altman,S.
TITLE Repeated sequences and open reading frames in the 3' flanking
region of the gene for the RNA subunit of Escherichia coli
ribonuclease P
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 80, 5359-5363 (1983)
STANDARD simple staff_entry
REFERENCE 2 (sites)
AUTHORS Schweizer,H. and Datta,P.
TITLE The complete nucleotide sequence of the tdc region of Escherichia
coli
JOURNAL Nucleic Acids Res. 17, 3994-3994 (1989)
STANDARD full staff_review
REFERENCE 3 (bases 1 to 4434)
AUTHORS Komine,Y. and Inokuchi,H.
TITLE Precise mapping of the rnpB gene encoding the RNA component of
RNase P in Escherichia coli K-12
JOURNAL J. Bacteriol. 173, 1813-1816 (1991)
STANDARD full staff_entry
COMMENT
[Nucleic Acids Res. 17, 3994-3994 (1989)] sites.
These data kindly submitted in computer readable form by:
Yuriko Komine
Department of Biophyics
Faculty of Science, Kyoto University
Sakyo-ku, Kyoto 606
Japan
Phone: 075-753-4200
Fax: 075-791-0271
FEATURES Location/Qualifiers
CDS <1..507
/note="ORF1 (AA at 1)"
CDS 528..1427
/note="ORF2"
CDS 1443..2669
/note="ORF3"
misc_RNA 2702..3078
/note="mature M1-RNA"
variation 3201..3201
/note="a in [J. Bacteriol. (1990) In press]; deletion in
[Proc. Natl. Acad. Sci. U.S.A. 80, 5359-5363 (1983)]"
variation 3278..3278
/note="a in [J. Bacteriol. (1990) In press]; deletion in
[Proc. Natl. Acad. Sci. U.S.A. 80, 5359-5363 (1983)]"
variation 3313..3313
/note="t in [J. Bacteriol. (1990) In press]; ta in [Proc.
Natl. Acad. Sci. U.S.A. 80, 5359-5363 (1983)]"
variation 3332..3332
/note="g in [J. Bacteriol. (1990) In press]; gt in [Proc.
Natl. Acad. Sci. U.S.A. 80, 5359-5363 (1983)]"
variation 3661..3661
/note="a in [J. Bacteriol. (1990) In press]; deletion in
[Proc. Natl. Acad. Sci. U.S.A. 80, 5359-5363 (1983)]"
CDS complement(3691..>4434)
/note="ORFX"
BASE COUNT 1130 a 980 c 1210 g 1114 t
ORIGIN 68 min on K12 map.
1 gatatcggtt tctataactt cctgattcct tttgtagaaa caaaagagga agcagagctg
61 gcggtggcat caacccgtta cccaccggaa ggcattcgcg gcgtctccgt ttctcaccgc
121 gccaatatgt ttggcaccgt ggcggattat ttcgctcagt cgaacaagaa catcactatt
181 ctggtccaga tagaaagtca gcagggcgta gataacgtcg atgccattgc cgctaccgaa
241 ggcgtagacg gcatcttcgt cggccccagc gatctggccg cggcattagg ccatctcggc
301 aatgcatcac acccggatgt acaaaaagca attcagcaca tttttaaccg tgccagcgcg
361 cacggcaaac ccagcggtat cctcgcgccg gtcgaagccg atgcgcgtcg ttatctggaa
421 tggggcgcga cgtttgtggc tgtcggcagc gatctcggcg tcttccgctc tgccactcag
481 aaactggctg atacctttaa aaaataacca ccaccgcaac aagagagatg attgatatga
541 ctatgaaagt tggttttatt ggcctgggga ttatgggtaa accaatgagt aaaaaccttc
601 tgaaagcagg ttactcgctg gtggttgctg accgtaaccc agaagctatt gctgacgtga
661 ttgctgcagg tgcagaaaca gcgtctacgg ctaaagcgat cgctgaacag tgcgacgtca
721 tcataaccat gctgccaaac tcccctcatg tgaaagaggt ggcgctgggt gagaatggca
781 ttattgaagg cgcgaagcca ggtacggtat tgatcgatat gagttctatc gcaccgctgg
841 caagccgtga aatcagcgaa gcgctgaaag cgaaaggcat tgatatgctg gatgctccgg
901 tgagcggcgg tgaaccgaaa gccatcgacg gtacgctgtc agtgatggtg ggcggcgaca
961 aggctatttt cgacaaatac tatgatttga tgaaagcgat ggcgggttcc gtggtgcata
1021 ccggggaaat cggtgcaggt aacgtcacca aactggcaaa tcaggtcatt gtggcgctga
1081 atattgccgc gatgtcagaa gcgttaacgc tggcaactaa agcgggcgtt aacccggacc
1141 tggtttatca ggcaattcgc ggtggactgg cgggcagtac cgtgctggat gccaaagcgc
1201 cgatggtgat ggaccgcaac ttcaagccgg gcttccgtat tgatctgcat attaaggatc
1261 tggcgaatgc gctggatact tctcacggcg tcggcgcaca actgccgctc acagctgcgg
1321 ttatggagat gatgcaggca ctgcgagcag atggtttagg aacggcggat catagcgccc
1381 tggcgtgcta ctacgaaaaa ctggcgaaag tcgaagttac tcgttaatga cgtcgtgccc
1441 ggatggcgta ttgcaatccg ggcctggaat ccaggccgaa taagagaaac gccctccggc
1501 gtcatgtggt aacaggcata ggtatgaaaa tcgtaatcgc cccagactct tataaagaaa
1561 gtttatctgc cagcgaggtt gcgcaggcga tagaaaaagg atttcgggaa atttttcctg
1621 atgcacagta cgtttctgtt ccggttgccg acggtggcga aggaacggtg gaagcgatga
1681 ttgcagccac ccagggggct gaacgtcacg cctgggttac agggccgctg ggcgagaaag
1741 tgaatgccag ttgggggatc tccggcgatg gcaaaaccgc gtttattgaa atggcggcgg
1801 ccagtgggct ggagctggta cctgcggaaa aacgcgatcc actcgtgacc acttcacgcg
1861 gcacaggcga gttaatcctg caggcgctgg agagcggtgc gacaaacatt attatcggca
1921 ttggcggcag cgctacaaat gatggcggcg caggcatggt acaggcgctg ggggcgaaat
1981 tatgcgacgc caacggcaat gaaattggtt ttggcggcgg tagtcttaat actctgaatg
2041 atattgatat ttccggcctc gatccgcgct taaaagattg cgtcattcgc gtcgcttgtg
2101 atgtcaccaa tccgctggtg ggcgataacg gcgcatcgcg catctttggc ccacaaaagg
2161 gagccagtga agcgatgatt gttgagctgg acaataacct ctctcactat gccgaggtca
2221 ttaaaaaagc gctgcatgtt gatgtgaaag atgtccccgg tgcaggagct gcgggtggta
2281 tgggcgcggc gctaatggcg tttcttggtg cggaactgaa aagtggtatt gaaatcgtca
2341 ctacggcgct gaatctggag gaacatattc acgattgtac gctggtgatc accggtgaag
2401 ggcgtattga cagccagagt attcacggga aggtaccgat tggtgtcgca aacgtggcga
2461 agaagtacca taaaccggtg attggcattg cgggtagcct gaccgatgat gttggcgttg
2521 tacatcagca tggcattgat gcggtcttca gcgtattgac cagcataggt acgttggacg
2581 aagcattccg cggggcttat gacaatatct gccgtgcttc acgtaatatc gccgcgacac
2641 tggcgattgg aatgcgcaac gcggggtgac aagggcgcgc aaaccctcta tactgcgcgc
2701 cgaagctgac cagacagtcg ccgcttcgtc gtcgtcctct tcgggggaga cgggcggagg
2761 ggaggaaagt ccgggctcca tagggcaggg tgccaggtaa cgcctggggg ggaaacccac
2821 gaccagtgca acagagagca aaccgccgat ggcccgcgca agcgggatca ggtaagggtg
2881 aaagggtgcg gtaagagcgc accgcgcggc tggtaacagt ccgtggcacg gtaaactcca
2941 cccggagcaa ggccaaatag gggttcataa ggtacggccc gtactgaacc cgggtaggct
3001 gcttgagcca gtgagcgatt gctggcctag atgaatgact gtccacgaca gaacccggct
3061 tatcggtcag tttcacctga tttacgtaaa aacccgcttc ggcgggtttt tgcttttgga
3121 ggggcagaaa gatgaatgac tgtccacgac gctataccca aaagaaagcg gcttatcggt
3181 cagtttcacc tggtttacgt aaaaacccgc ttcggcgggt ttttgctttt ggaggggcag
3241 aaagatgaat gactgtccac gacactatac ccaaaagaaa gcggcttatc ggtcagtttc
3301 acctgtttta cgtaaaaacc cgcttcggcg ggttttactt ttggaggggc agaaagatga
3361 atgactgtcc acgacactat acccaaaaga aagcggctta tcggtcagtt ttacctgatg
3421 tacgtaataa accgttccgg cgggtttcag attgttgagt gcgctttatt catgccggat
3481 gcggcgtgaa cgccttatcc tgcctacaaa agcacgcaaa ttcaatatat tgcagagatg
3541 gcgtaggcct gataagcgta gcgcatcagg catttttgcg gttgccatca gtttcaaacc
3601 cgcgtcagtg agtttttgct tttggtgcac agggtaagat gaataaatat ctacggtatt
3661 aataatagac tttgtaagaa gttattaagt ctatattgca tttaaaaggt cgtaatcata
3721 cgtatagtcc tctctggtaa aatattgctt ctctgagtta tatgcttcat atgtgttata
3781 ttccccgacc tcaaagccat tgtccggaga attttcgtct ttctcgaggg tattatctga
3841 aagtaactct tctgagctag agtcttctat gtttaaatcc atagtataat taatttgggg
3901 gacgaccaga taagcatttt ctttggtatt ttcataatct tcaatgatta tatcgatgta
3961 atcatatatt ttttctgatg agaattcttt catctctgtt aatttaattc tgttcaagca
4021 attgatgatt ttcgatgagc ttttatatat ttcttttgtt aattcatttt ctaatttatc
4081 tgtaagagtt gcccttggta tagattttat aattgcaaat ctcataattt ttaattgttc
4141 ttcggtaatg tctccaggct catttaaaca actttcgaca aacttgattt tttcttcaga
4201 atacatatcg ctaatgctta ttaaaacagg cgttattttt agtaaataat cccaggagat
4261 tgtggcgttt tgtgttgaac taacaaaatt aacctgaaac atgttggcat tagtaaaatt
4321 tgttgaattc aatttaaaag gatttaaaat agtattagcc atatgtttat ctgtgaaatc
4381 ataataatct aaatcgacat tacgtaagtc cagagaggat agatcaacga tatc
//