GenBank-Updates@genbank.bio.net (01/08/91)
LOCUS PMMB190 9065 bp ds-DNA Circular SYN 08-JAN-1991
DEFINITION Bacterial cloning vector pMMB190, complete genome.
ACCESSION M37846
KEYWORDS DNA helicase; alpha peptide; beta-lactamase; lac repressor protein;
primase; regulatory protein; replication initiator.
SOURCE Bacterial cloning vector pMMB190.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (bases 1 to 9065)
AUTHORS Morales,V.M., Backman,A. and Bagdasarian,M.
TITLE A series of broad-host-range low-copy-number cloning vectors that
allow direct screening for recombinants
JOURNAL Unpublished (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by V.M.Morales, 21-AUG-1990.
Natl Res Council, Canada
Plant Biotech Inst
Saskatoon, Sask.
S7N OW9 Canada
FEATURES Location/Qualifiers
CDS join(9047..9063,1..181)
/gene="lacZ'"
/evidence=EXPERIMENTAL
/product="alfa peptide"
promoter 638..668
/gene="bla"
CDS 708..1568
/gene="bla"
/codon_start=708
/product="beta-lactamase"
rep_origin 2006..2400
CDS complement(2426..2710)
/function="conjugational DNA transfer"
/gene="mobC"
/codon_start=2426
misc_feature 2663..2820
/note="origin of conjugational DNA transfer"
promoter 2702..2732
/note="P1"
promoter 2721..2751
/note="P3"
promoter 2772..2743
/note="P2"
CDS 2909..5038
/gene="repB/mobA"
/codon_start=2909
/product="primase"
CDS 3657..4070
/gene="mobB"
/codon_start=3657
/product="conjugational DNA transfer protein"
CDS 4067..5038
/gene="repB'"
/codon_start=4067
/product="primase"
promoter 5040..5071
/note="P4"
CDS 5099..5311
/note="protein of unknown function"
/gene="E"
/codon_start=5099
CDS 5313..5519
/note="regulates repA and repC gene expression."
/gene="cac"
/codon_start=5313
/product="regulatory protein"
CDS 5549..6388
/gene="repA"
/codon_start=5549
/product="DNA helicase"
CDS 6375..7226
/note="contains stop codons."
/gene="repC"
/codon_start=6375
/product="replication initiator protein"
promoter 7387..7417
/note="P5"
CDS complement(7503..8585)
/note="contains stop codons."
/gene="lacI"
/codon_start=7503
/product="lac repressor"
promoter 8649..8620
/gene="lacIq"
promoter 8893..8921
/gene="tac"
promoter 8975..9004
/gene="lacUV5"
BASE COUNT 1962 a 2654 c 2692 g 1757 t
ORIGIN
1 ttcccgggga tccgtcgacc tgcagccaag cttggcactg gccgtcgttt tacaacgtcg
61 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
121 cagctggcta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
181 aatggcgatg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
241 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
301 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
361 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
421 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
481 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
541 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
601 gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
661 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
721 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
781 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
841 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
901 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
961 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
1021 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
1081 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
1141 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
1201 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
1261 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
1321 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
1381 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
1441 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
1501 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
1561 attggtaact gtcagaccaa gtttactcat atatacttta gattgatttc tgaaagcgac
1621 caggtgctcg gcgtggcaag actcgcagcg aacccgtaga aagccatgct ccagccgccc
1681 gcattggaga aattcttcaa attcccgttg cacatagccc ggcaattcct ttccctgctc
1741 tgccataagc gcagcgaatg ccgggtaata ctcgtcaacg atctgataga gaagggtttg
1801 ctcgggtcgg tggctctggt aacgaccagt atcccgatcc cggctggccg tcctggccgc
1861 cacatgaggc atgttccgcg tccttgcaat actgtgttta catacagtct atcgcttagc
1921 ggaaagttct tttaccctca gccgaaatgc ctgccgttgc tagacattgc cagccagtgc
1981 ccgtcactcc cgtactaact gtcacgaacc cctgcaataa ctgtcacgcc cccctgcaat
2041 aactgtcacg aacccctgca ataactgtca cgcccccaaa cctgcaaacc cagcaggggc
2101 gggggctggc ggggtgttgg aaaaatccat ccatgattat ctaagaataa tccactaggc
2161 gcggttatca gcgcccttgt ggggcgctgc tgcccttgcc caatatgccc ggccagaggc
2221 cggatagctg gtctattcgc tgcgctaggc tacacaccgc cccaccgctg cgcggcaggg
2281 ggaaaggcgg gcaaagcccg ctaaacccca caccaaaccc cgcagaaata cgctggagcg
2341 cttttagccg ctttagcggc ctttccccct acccgaaggg tgggggcgcg tgtgcagccc
2401 cgcagggcct gtctcggtcg atcattcagc ccggctcatc cttctggcgt ggcggcagac
2461 cgaacaaggc gcggtcgtgg tcgcgttcaa ggtacgcatc cattgccgcc atgagccgat
2521 cctccggcca ctcgctgctg ttcaccttgg ccaaaatcat ggcccccacc agcaccttgc
2581 gccttgtttc gttcttgcgc tcttgctgct gttcccttgc ccgctcccgc tgaatttcgg
2641 cattgattcg cgctcgttgt tcttcgagct tggccagccg atccgccgcc ttgttgctcc
2701 ccttaaccat cttgacaccc cattgttaat gtgctgtctc gtaggctatc atggaggcac
2761 agcggcggca atcccgaccc tactttgtag gggagggcgc acttaccggt ttctcttcga
2821 gaaactggcc taacggccac ccttcgggcg gtgcgctctc cgagggccat tgcatggagc
2881 cgaaaagcaa aagcaacagc gaggcagcat ggcgatttat caccttacgg cgaaaaccgg
2941 cagcaggtcg ggcggccaat cggccagggc caaggccgac tacatccagc gcgaaggcaa
3001 gtatgcccgc gacatggatg aagtcttgca cgccgaatcc gggcacatgc cggagttcgt
3061 cgagcggccc gccgactact gggatgctgc cgacctgtat gaacgcgcca atgggcggct
3121 gttcaaggag gtcgaatttg ccctgccggt cgagctgacc ctcgaccagc agaaggcgct
3181 ggcgtccgag ttcgcccagc acctgaccgg tgccgagcgc ctgccgtata cgctggccat
3241 ccatgccggt ggcggcgaga acccgcactg ccacctgatg atctccgagc ggatcaatga
3301 cggcatcgag cggcccgccg ctcagtggtt caagcggtac aacggcaaga ccccggagaa
3361 gggcggggca cagaagaccg aagcgctcaa gcccaaggca tggcttgagc agacccgcga
3421 ggcatgggcc gaccatgcca accgggcatt agagcgggct ggccacgacg cccgcattga
3481 ccacagaaca cttgaggcgc agggcatcga gcgcctgccc ggtgttcacc tggggccgaa
3541 cgtggtggag atggaaggcc ggggcatccg caccgaccgg gcagacgtgg ccctgaacat
3601 cgacaccgcc aacgcccaga tcatcgactt acaggaatac cgggaggcaa tagaccatga
3661 acgcaatcga cagagtgaag aaatccagag gcatcaacga gttagcggag cagatcgaac
3721 cgctggccca gagcatggcg acactggccg acgaagcccg gcaggtcatg agccagacca
3781 agcaggccag cgaggcgcag gcggcggagt ggctgaaagc ccagcgccag acaggggcgg
3841 catgggtgga gctggccaaa gagttgcggg aggtagccgc cgaggtgagc agcgccgcgc
3901 agagcgcccg gagcgcgtcg cgggggtggc actggaagct atggctaacc gtgatgctgg
3961 cttccatgat gcctacggtg gtgctgctga tcgcatcgtt gctcttgctc gacctgacgc
4021 cactgacaac cgaggacggc tcgatctggc tgcgcttggt ggcccgatga agaacgacag
4081 gactttgcag gccataggcc gacagctcaa ggccatgggc tgtgagcgct tcgatatcgg
4141 cgtcagggac gcacccaccg gccagatgat gaaccgggaa tggtcagccg ccgaagtgct
4201 ccagaacacg ccatggctca agcggatgaa tgcccagggc aatgacgtgt atatcaggcc
4261 cgccgagcag gagcggcatg gtctggtgct ggtggacgac ctcagcgagt ttgacctgga
4321 tgacatgaaa gccgagggcc gggagcctgc cctggtagtg gaaaccagcc cgaagaacta
4381 tcaggcatgg gtcaaggtgg ccgacgccgc aggcggtgaa cttcgggggc agattgcccg
4441 gacgctggcc agcgagtacg acgccgaccc ggccagcgcc gacagccgcc actatggccg
4501 cttggcgggc ttcaccaacc gcaaggacaa gcacaccacc cgcgccggtt atcagccgtg
4561 ggtgctgctg cgtgaatcca agggcaagac cgccaccgct ggcccggcgc tggtgcagca
4621 ggctggccag cagatcgagc aggcccagcg gcagcaggag aaggcccgca ggctggccag
4681 cctcgaactg cccgagcggc agcttagccg ccaccggcgc acggcgctgg acgagtaccg
4741 cagcgagatg gccgggctgg tcaagcgctt cggtcatgac ctcagcaagt gcgactttat
4801 cgccgcgcag aagctggcca gccggggccg cagtgccgag gaaatcggca aggccatggc
4861 cgaggccagc ccagcgctgg cagagcgcaa gcccggccac gaagcggatt acatcgagcg
4921 caccgtcagc aaggtcatgg gtctgcccag cgtccagctt gcgcgggccg agctggcacg
4981 ggcaccggca ccccgccagc gaggcatgga caggggcggg ccagatttca gcatgtagtg
5041 cttgcgttgg tactcacgcc tgttatacta tgagtactca cgcacagaag ggggttttat
5101 ggaatacgaa aaaagcgctt cagggtcggt ctacctgatc aaaagtgaca agggctattg
5161 gttgcccggt ggctttggtt atacgtcaaa caaggccgag gctggccgct tttcagtcgc
5221 tgatatggcc agccttaacc ttgacggctg caccttgtcc ttgttccgcg aagacaagcc
5281 tttcggcccc ggcaagtttc tcggtgactg atatgaaaga ccaaaaggac aagcagaccg
5341 gcgacctgct ggccagccct gacgctgtac gccaagcgcg atatgccgag cgcatgaagg
5401 ccaaagggat gcgtcagcgc aagttctggc tgaccgacga cgaatacgag gcgctgcgcg
5461 agtgcctgga agaactcaga gcggcgcagg gcgggggtag tgaccccgcc agcgcctaac
5521 caccaactgc ctgcaaagga ggcaatcaat ggctacccat aagcctatca atattctgga
5581 ggcgttcgca gcagcgccgc caccgctgga ctacgttttg cccaacatgg tggccggtac
5641 ggtcggggcg ctggtgtcgc ccggtggtgc cggtaaatcc atgctggccc tgcaactggc
5701 cgcacagatt gcaggcgggc cggatctgct ggaggtgggc gaactgccca ccggcccggt
5761 gatctacctg cccgccgaag acccgcccac cgccattcat caccgcctgc acgcccttgg
5821 ggcgcacctc agcgccgagg aacggcaagc cgtggctgac ggcctgctga tccagccgct
5881 gatcggcagc ctgcccaaca tcatggcccc ggagtggttc gacggcctca agcgcgccgc
5941 cgagggccgc cgcctgatgg tgctggacac gctgcgccgg ttccacatcg aggaagaaaa
6001 cgccagcggc cccatggccc aggtcatcgg tcgcatggag gccatcgccg ccgataccgg
6061 gtgctctatc gtgttcctgc accatgccag caagggcgcg gccatgatgg gcgcaggcga
6121 ccagcagcag gccagccggg gcagctcggt actggtcgat aacatccgct ggcagtccta
6181 cctgtcgagc atgaccagcg ccgaggccga ggaatggggt gtggacgacg accagcgccg
6241 gttcttcgtc cgcttcggtg tgagcaaggc caactatggc gcaccgttcg ctgatcggtg
6301 gttcaggcgg catgacggcg gggtgctcaa gcccgccgtg ctggagaggc agcgcaagag
6361 caagggggtg ccccgtggtg aagcctaaga acaagcacag cctcagccac gtccggcacg
6421 acccggcgca ctgtctggcc cccggcctgt tccgtgccct caagcggggc gagcgcaagc
6481 gcagcaagct ggacgtgacg tatgactacg gcgacggcaa gcggatcgag ttcagcggcc
6541 cggagccgct gggcgctgat gatctgcgca tcctgcaagg gctggtggcc atggctgggc
6601 ctaatggcct agtgcttggc ccggaaccca agaccgaagg cggacggcag ctccggctgt
6661 tcctggaacc caagtgggag gccgtcaccg ctgaatgcca tgtggtcaaa ggtagctatc
6721 gggcgctggc aaaggaaatc ggggcagagg tcgatagtgg tggggcgctc aagcacatac
6781 aggactgcat cgagcgcctt tggaaggtat ccatcatcgc ccagaatggc cgcaagcggc
6841 aggggtttcg gctgctgtcg gagtacgcca gcgacgaggc ggacgggcgc ctgtacgtgg
6901 ccctgaaccc cttgatcgcg caggccgtca tgggtggcgg ccagcatgtg cgcatcagca
6961 tggacgaggt gcgggcgctg gacagcgaaa ccgcccgcct gctgcaccag cggctgtgtg
7021 gctggatcga ccccggcaaa accggcaagg cttccataga taccttgtgc ggctatgtct
7081 ggccgtcaga ggccagtggt tcgaccatgc gcaagcgccg caagcgggtg cgcgaggcgt
7141 tgccggagct ggtcgcgctg ggctggacgg taaccgagtt cgcggcgggc aagtacgaca
7201 tcacccggcc caaggcggca ggctgacccc ccccactcta ttgtaaacaa gacattttta
7261 tcttttatat tcaatggctt attttcctgc taattggtaa taccatgaaa aataccatgc
7321 tcagaaaagg cttaacaata ttttgaaaaa ttgcctactg agcgctgccg cacagctcca
7381 taggccgctt tcctggcttt gcttccagat gtatgctctt ctgctcccga acgccagcaa
7441 gacgtagccc agcgcgtcgg ccagcttgca attcgcgcta acttacatta attgcgttgc
7501 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
7561 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
7621 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
7681 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
7741 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc
7801 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
7861 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
7921 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
7981 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
8041 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg
8101 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
8161 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
8221 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
8281 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
8341 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
8401 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
8461 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
8521 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
8581 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
8641 gttttgcacc attcgatggt gtcaacgtaa atgccgcttc gccttcgcgc gcgaattgca
8701 agctgatccg ggcttatcga ctgcacggtg caccaatgct tctggcgtca ggctgccatc
8761 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
8821 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
8881 tgaaatgagc tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat
8941 aacaatttca cacaggaaac taggcacccc aggctttaca ctttatgctt ccggctcgta
9001 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
9061 acgaa
//GenBank-Updates@genbank.bio.net (01/15/91)
LOCUS PMMB190 9065 bp ds-DNA Circular SYN 14-JAN-1991
DEFINITION Bacterial cloning vector pMMB190, complete genome.
ACCESSION M37846
KEYWORDS DNA helicase; alpha peptide; beta-lactamase; lac repressor protein;
primase; regulatory protein; replication initiator.
SOURCE Bacterial cloning vector pMMB190.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (bases 1 to 9065)
AUTHORS Morales,V.M., Backman,A. and Bagdasarian,M.
TITLE A series of broad-host-range low-copy-number cloning vectors that
allow direct screening for recombinants
JOURNAL Unpublished (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by V.M.Morales, 21-AUG-1990.
Natl Res Council, Canada
Plant Biotech Inst
Saskatoon, Sask.
S7N OW9 Canada
FEATURES Location/Qualifiers
CDS join(9047..9063,1..181)
/gene="lacZ'"
/evidence=EXPERIMENTAL
/product="alfa peptide"
promoter 638..668
/gene="bla"
CDS 708..1568
/gene="bla"
/codon_start=708
/product="beta-lactamase"
rep_origin 2006..2400
CDS complement(2426..2710)
/function="conjugational DNA transfer"
/gene="mobC"
/codon_start=2426
misc_feature 2663..2820
/note="origin of conjugational DNA transfer"
promoter 2702..2732
/note="P1"
promoter 2721..2751
/note="P3"
promoter 2772..2743
/note="P2"
CDS 2909..5038
/gene="repB/mobA"
/codon_start=2909
/product="primase"
CDS 3657..4070
/gene="mobB"
/codon_start=3657
/product="conjugational DNA transfer protein"
CDS 4067..5038
/gene="repB'"
/codon_start=4067
/product="primase"
promoter 5040..5071
/note="P4"
CDS 5099..5311
/note="protein of unknown function"
/gene="E"
/codon_start=5099
CDS 5313..5519
/note="regulates repA and repC gene expression."
/gene="cac"
/codon_start=5313
/product="regulatory protein"
CDS 5549..6388
/gene="repA"
/codon_start=5549
/product="DNA helicase"
CDS 6375..7226
/note="contains stop codons."
/gene="repC"
/codon_start=6375
/product="replication initiator protein"
promoter 7387..7417
/note="P5"
CDS complement(7503..8585)
/note="contains stop codons."
/gene="lacI"
/codon_start=7503
/product="lac repressor"
promoter 8649..8620
/gene="lacIq"
promoter 8893..8921
/gene="tac"
promoter 8975..9004
/gene="lacUV5"
BASE COUNT 1962 a 2654 c 2692 g 1757 t
ORIGIN
1 ttcccgggga tccgtcgacc tgcagccaag cttggcactg gccgtcgttt tacaacgtcg
61 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
121 cagctggcta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
181 aatggcgatg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
241 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
301 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
361 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
421 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
481 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
541 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
601 gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
661 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
721 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
781 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
841 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
901 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
961 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
1021 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
1081 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
1141 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
1201 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
1261 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
1321 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
1381 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
1441 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
1501 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
1561 attggtaact gtcagaccaa gtttactcat atatacttta gattgatttc tgaaagcgac
1621 caggtgctcg gcgtggcaag actcgcagcg aacccgtaga aagccatgct ccagccgccc
1681 gcattggaga aattcttcaa attcccgttg cacatagccc ggcaattcct ttccctgctc
1741 tgccataagc gcagcgaatg ccgggtaata ctcgtcaacg atctgataga gaagggtttg
1801 ctcgggtcgg tggctctggt aacgaccagt atcccgatcc cggctggccg tcctggccgc
1861 cacatgaggc atgttccgcg tccttgcaat actgtgttta catacagtct atcgcttagc
1921 ggaaagttct tttaccctca gccgaaatgc ctgccgttgc tagacattgc cagccagtgc
1981 ccgtcactcc cgtactaact gtcacgaacc cctgcaataa ctgtcacgcc cccctgcaat
2041 aactgtcacg aacccctgca ataactgtca cgcccccaaa cctgcaaacc cagcaggggc
2101 gggggctggc ggggtgttgg aaaaatccat ccatgattat ctaagaataa tccactaggc
2161 gcggttatca gcgcccttgt ggggcgctgc tgcccttgcc caatatgccc ggccagaggc
2221 cggatagctg gtctattcgc tgcgctaggc tacacaccgc cccaccgctg cgcggcaggg
2281 ggaaaggcgg gcaaagcccg ctaaacccca caccaaaccc cgcagaaata cgctggagcg
2341 cttttagccg ctttagcggc ctttccccct acccgaaggg tgggggcgcg tgtgcagccc
2401 cgcagggcct gtctcggtcg atcattcagc ccggctcatc cttctggcgt ggcggcagac
2461 cgaacaaggc gcggtcgtgg tcgcgttcaa ggtacgcatc cattgccgcc atgagccgat
2521 cctccggcca ctcgctgctg ttcaccttgg ccaaaatcat ggcccccacc agcaccttgc
2581 gccttgtttc gttcttgcgc tcttgctgct gttcccttgc ccgctcccgc tgaatttcgg
2641 cattgattcg cgctcgttgt tcttcgagct tggccagccg atccgccgcc ttgttgctcc
2701 ccttaaccat cttgacaccc cattgttaat gtgctgtctc gtaggctatc atggaggcac
2761 agcggcggca atcccgaccc tactttgtag gggagggcgc acttaccggt ttctcttcga
2821 gaaactggcc taacggccac ccttcgggcg gtgcgctctc cgagggccat tgcatggagc
2881 cgaaaagcaa aagcaacagc gaggcagcat ggcgatttat caccttacgg cgaaaaccgg
2941 cagcaggtcg ggcggccaat cggccagggc caaggccgac tacatccagc gcgaaggcaa
3001 gtatgcccgc gacatggatg aagtcttgca cgccgaatcc gggcacatgc cggagttcgt
3061 cgagcggccc gccgactact gggatgctgc cgacctgtat gaacgcgcca atgggcggct
3121 gttcaaggag gtcgaatttg ccctgccggt cgagctgacc ctcgaccagc agaaggcgct
3181 ggcgtccgag ttcgcccagc acctgaccgg tgccgagcgc ctgccgtata cgctggccat
3241 ccatgccggt ggcggcgaga acccgcactg ccacctgatg atctccgagc ggatcaatga
3301 cggcatcgag cggcccgccg ctcagtggtt caagcggtac aacggcaaga ccccggagaa
3361 gggcggggca cagaagaccg aagcgctcaa gcccaaggca tggcttgagc agacccgcga
3421 ggcatgggcc gaccatgcca accgggcatt agagcgggct ggccacgacg cccgcattga
3481 ccacagaaca cttgaggcgc agggcatcga gcgcctgccc ggtgttcacc tggggccgaa
3541 cgtggtggag atggaaggcc ggggcatccg caccgaccgg gcagacgtgg ccctgaacat
3601 cgacaccgcc aacgcccaga tcatcgactt acaggaatac cgggaggcaa tagaccatga
3661 acgcaatcga cagagtgaag aaatccagag gcatcaacga gttagcggag cagatcgaac
3721 cgctggccca gagcatggcg acactggccg acgaagcccg gcaggtcatg agccagacca
3781 agcaggccag cgaggcgcag gcggcggagt ggctgaaagc ccagcgccag acaggggcgg
3841 catgggtgga gctggccaaa gagttgcggg aggtagccgc cgaggtgagc agcgccgcgc
3901 agagcgcccg gagcgcgtcg cgggggtggc actggaagct atggctaacc gtgatgctgg
3961 cttccatgat gcctacggtg gtgctgctga tcgcatcgtt gctcttgctc gacctgacgc
4021 cactgacaac cgaggacggc tcgatctggc tgcgcttggt ggcccgatga agaacgacag
4081 gactttgcag gccataggcc gacagctcaa ggccatgggc tgtgagcgct tcgatatcgg
4141 cgtcagggac gcacccaccg gccagatgat gaaccgggaa tggtcagccg ccgaagtgct
4201 ccagaacacg ccatggctca agcggatgaa tgcccagggc aatgacgtgt atatcaggcc
4261 cgccgagcag gagcggcatg gtctggtgct ggtggacgac ctcagcgagt ttgacctgga
4321 tgacatgaaa gccgagggcc gggagcctgc cctggtagtg gaaaccagcc cgaagaacta
4381 tcaggcatgg gtcaaggtgg ccgacgccgc aggcggtgaa cttcgggggc agattgcccg
4441 gacgctggcc agcgagtacg acgccgaccc ggccagcgcc gacagccgcc actatggccg
4501 cttggcgggc ttcaccaacc gcaaggacaa gcacaccacc cgcgccggtt atcagccgtg
4561 ggtgctgctg cgtgaatcca agggcaagac cgccaccgct ggcccggcgc tggtgcagca
4621 ggctggccag cagatcgagc aggcccagcg gcagcaggag aaggcccgca ggctggccag
4681 cctcgaactg cccgagcggc agcttagccg ccaccggcgc acggcgctgg acgagtaccg
4741 cagcgagatg gccgggctgg tcaagcgctt cggtcatgac ctcagcaagt gcgactttat
4801 cgccgcgcag aagctggcca gccggggccg cagtgccgag gaaatcggca aggccatggc
4861 cgaggccagc ccagcgctgg cagagcgcaa gcccggccac gaagcggatt acatcgagcg
4921 caccgtcagc aaggtcatgg gtctgcccag cgtccagctt gcgcgggccg agctggcacg
4981 ggcaccggca ccccgccagc gaggcatgga caggggcggg ccagatttca gcatgtagtg
5041 cttgcgttgg tactcacgcc tgttatacta tgagtactca cgcacagaag ggggttttat
5101 ggaatacgaa aaaagcgctt cagggtcggt ctacctgatc aaaagtgaca agggctattg
5161 gttgcccggt ggctttggtt atacgtcaaa caaggccgag gctggccgct tttcagtcgc
5221 tgatatggcc agccttaacc ttgacggctg caccttgtcc ttgttccgcg aagacaagcc
5281 tttcggcccc ggcaagtttc tcggtgactg atatgaaaga ccaaaaggac aagcagaccg
5341 gcgacctgct ggccagccct gacgctgtac gccaagcgcg atatgccgag cgcatgaagg
5401 ccaaagggat gcgtcagcgc aagttctggc tgaccgacga cgaatacgag gcgctgcgcg
5461 agtgcctgga agaactcaga gcggcgcagg gcgggggtag tgaccccgcc agcgcctaac
5521 caccaactgc ctgcaaagga ggcaatcaat ggctacccat aagcctatca atattctgga
5581 ggcgttcgca gcagcgccgc caccgctgga ctacgttttg cccaacatgg tggccggtac
5641 ggtcggggcg ctggtgtcgc ccggtggtgc cggtaaatcc atgctggccc tgcaactggc
5701 cgcacagatt gcaggcgggc cggatctgct ggaggtgggc gaactgccca ccggcccggt
5761 gatctacctg cccgccgaag acccgcccac cgccattcat caccgcctgc acgcccttgg
5821 ggcgcacctc agcgccgagg aacggcaagc cgtggctgac ggcctgctga tccagccgct
5881 gatcggcagc ctgcccaaca tcatggcccc ggagtggttc gacggcctca agcgcgccgc
5941 cgagggccgc cgcctgatgg tgctggacac gctgcgccgg ttccacatcg aggaagaaaa
6001 cgccagcggc cccatggccc aggtcatcgg tcgcatggag gccatcgccg ccgataccgg
6061 gtgctctatc gtgttcctgc accatgccag caagggcgcg gccatgatgg gcgcaggcga
6121 ccagcagcag gccagccggg gcagctcggt actggtcgat aacatccgct ggcagtccta
6181 cctgtcgagc atgaccagcg ccgaggccga ggaatggggt gtggacgacg accagcgccg
6241 gttcttcgtc cgcttcggtg tgagcaaggc caactatggc gcaccgttcg ctgatcggtg
6301 gttcaggcgg catgacggcg gggtgctcaa gcccgccgtg ctggagaggc agcgcaagag
6361 caagggggtg ccccgtggtg aagcctaaga acaagcacag cctcagccac gtccggcacg
6421 acccggcgca ctgtctggcc cccggcctgt tccgtgccct caagcggggc gagcgcaagc
6481 gcagcaagct ggacgtgacg tatgactacg gcgacggcaa gcggatcgag ttcagcggcc
6541 cggagccgct gggcgctgat gatctgcgca tcctgcaagg gctggtggcc atggctgggc
6601 ctaatggcct agtgcttggc ccggaaccca agaccgaagg cggacggcag ctccggctgt
6661 tcctggaacc caagtgggag gccgtcaccg ctgaatgcca tgtggtcaaa ggtagctatc
6721 gggcgctggc aaaggaaatc ggggcagagg tcgatagtgg tggggcgctc aagcacatac
6781 aggactgcat cgagcgcctt tggaaggtat ccatcatcgc ccagaatggc cgcaagcggc
6841 aggggtttcg gctgctgtcg gagtacgcca gcgacgaggc ggacgggcgc ctgtacgtgg
6901 ccctgaaccc cttgatcgcg caggccgtca tgggtggcgg ccagcatgtg cgcatcagca
6961 tggacgaggt gcgggcgctg gacagcgaaa ccgcccgcct gctgcaccag cggctgtgtg
7021 gctggatcga ccccggcaaa accggcaagg cttccataga taccttgtgc ggctatgtct
7081 ggccgtcaga ggccagtggt tcgaccatgc gcaagcgccg caagcgggtg cgcgaggcgt
7141 tgccggagct ggtcgcgctg ggctggacgg taaccgagtt cgcggcgggc aagtacgaca
7201 tcacccggcc caaggcggca ggctgacccc ccccactcta ttgtaaacaa gacattttta
7261 tcttttatat tcaatggctt attttcctgc taattggtaa taccatgaaa aataccatgc
7321 tcagaaaagg cttaacaata ttttgaaaaa ttgcctactg agcgctgccg cacagctcca
7381 taggccgctt tcctggcttt gcttccagat gtatgctctt ctgctcccga acgccagcaa
7441 gacgtagccc agcgcgtcgg ccagcttgca attcgcgcta acttacatta attgcgttgc
7501 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
7561 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
7621 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
7681 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
7741 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc
7801 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
7861 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
7921 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
7981 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
8041 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg
8101 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
8161 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
8221 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
8281 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
8341 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
8401 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
8461 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
8521 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
8581 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
8641 gttttgcacc attcgatggt gtcaacgtaa atgccgcttc gccttcgcgc gcgaattgca
8701 agctgatccg ggcttatcga ctgcacggtg caccaatgct tctggcgtca ggctgccatc
8761 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
8821 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
8881 tgaaatgagc tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat
8941 aacaatttca cacaggaaac taggcacccc aggctttaca ctttatgctt ccggctcgta
9001 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
9061 acgaa
//GenBank-Updates@genbank.bio.net (03/02/91)
LOCUS PMMB190 9065 bp ds-DNA Circular SYN 02-MAR-1991
DEFINITION Bacterial cloning vector pMMB190, complete genome.
ACCESSION M37846
KEYWORDS DNA helicase; alpha peptide; beta-lactamase; lac repressor protein;
primase; regulatory protein; replication initiator.
SOURCE Bacterial cloning vector pMMB190.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (bases 1 to 9065)
AUTHORS Morales,V.M., Backman,A. and Bagdasarian,M.
TITLE A series of broad-host-range low-copy-number cloning vectors that
allow direct screening for recombinants
JOURNAL Unpublished (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by V.M.Morales, 21-AUG-1990.
Natl Res Council, Canada
Plant Biotech Inst
Saskatoon, Sask.
S7N OW9 Canada
FEATURES Location/Qualifiers
CDS join(9047..9063,1..181)
/product="alfa peptide"
/gene="lacZ'"
/evidence=EXPERIMENTAL
/codon_start=9047
promoter 638..668
/gene="bla"
CDS 708..1568
/product="beta-lactamase"
/gene="bla"
/codon_start=708
rep_origin 2006..2400
CDS complement(2426..2710)
/function="conjugational DNA transfer"
/gene="mobC"
/codon_start=2708
misc_feature 2663..2820
/note="origin of conjugational DNA transfer"
promoter 2702..2732
/note="P1"
promoter 2721..2751
/note="P3"
CDS 2909..5038
/product="primase"
/gene="repB/mobA"
/codon_start=2909
CDS 3657..4070
/product="conjugational DNA transfer protein"
/gene="mobB"
/codon_start=3657
CDS 4067..5038
/product="primase"
/gene="repB'"
/codon_start=4067
promoter 5040..5071
/note="P4"
CDS 5099..5311
/note="protein of unknown function"
/gene="E"
/codon_start=5099
CDS 5313..5519
/note="regulates repA and repC gene expression."
/product="regulatory protein"
/gene="cac"
/codon_start=5313
CDS 5549..6388
/product="DNA helicase"
/gene="repA"
/codon_start=5549
CDS 6375..7226
/note="contains stop codons."
/product="replication initiator protein"
/gene="repC"
/codon_start=6375
promoter 7387..7417
/note="P5"
CDS complement(7503..8585)
/note="contains stop codons."
/product="lac repressor"
/gene="lacI"
/codon_start=8583
promoter 8620..8649
/gene="lacIq"
promoter 8893..8921
/gene="tac"
promoter 8975..9004
/gene="lacUV5"
BASE COUNT 1962 a 2654 c 2692 g 1757 t
ORIGIN
1 ttcccgggga tccgtcgacc tgcagccaag cttggcactg gccgtcgttt tacaacgtcg
61 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
121 cagctggcta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
181 aatggcgatg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
241 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
301 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
361 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
421 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
481 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
541 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
601 gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
661 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
721 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
781 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
841 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
901 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
961 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
1021 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
1081 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
1141 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
1201 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
1261 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
1321 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
1381 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
1441 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
1501 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
1561 attggtaact gtcagaccaa gtttactcat atatacttta gattgatttc tgaaagcgac
1621 caggtgctcg gcgtggcaag actcgcagcg aacccgtaga aagccatgct ccagccgccc
1681 gcattggaga aattcttcaa attcccgttg cacatagccc ggcaattcct ttccctgctc
1741 tgccataagc gcagcgaatg ccgggtaata ctcgtcaacg atctgataga gaagggtttg
1801 ctcgggtcgg tggctctggt aacgaccagt atcccgatcc cggctggccg tcctggccgc
1861 cacatgaggc atgttccgcg tccttgcaat actgtgttta catacagtct atcgcttagc
1921 ggaaagttct tttaccctca gccgaaatgc ctgccgttgc tagacattgc cagccagtgc
1981 ccgtcactcc cgtactaact gtcacgaacc cctgcaataa ctgtcacgcc cccctgcaat
2041 aactgtcacg aacccctgca ataactgtca cgcccccaaa cctgcaaacc cagcaggggc
2101 gggggctggc ggggtgttgg aaaaatccat ccatgattat ctaagaataa tccactaggc
2161 gcggttatca gcgcccttgt ggggcgctgc tgcccttgcc caatatgccc ggccagaggc
2221 cggatagctg gtctattcgc tgcgctaggc tacacaccgc cccaccgctg cgcggcaggg
2281 ggaaaggcgg gcaaagcccg ctaaacccca caccaaaccc cgcagaaata cgctggagcg
2341 cttttagccg ctttagcggc ctttccccct acccgaaggg tgggggcgcg tgtgcagccc
2401 cgcagggcct gtctcggtcg atcattcagc ccggctcatc cttctggcgt ggcggcagac
2461 cgaacaaggc gcggtcgtgg tcgcgttcaa ggtacgcatc cattgccgcc atgagccgat
2521 cctccggcca ctcgctgctg ttcaccttgg ccaaaatcat ggcccccacc agcaccttgc
2581 gccttgtttc gttcttgcgc tcttgctgct gttcccttgc ccgctcccgc tgaatttcgg
2641 cattgattcg cgctcgttgt tcttcgagct tggccagccg atccgccgcc ttgttgctcc
2701 ccttaaccat cttgacaccc cattgttaat gtgctgtctc gtaggctatc atggaggcac
2761 agcggcggca atcccgaccc tactttgtag gggagggcgc acttaccggt ttctcttcga
2821 gaaactggcc taacggccac ccttcgggcg gtgcgctctc cgagggccat tgcatggagc
2881 cgaaaagcaa aagcaacagc gaggcagcat ggcgatttat caccttacgg cgaaaaccgg
2941 cagcaggtcg ggcggccaat cggccagggc caaggccgac tacatccagc gcgaaggcaa
3001 gtatgcccgc gacatggatg aagtcttgca cgccgaatcc gggcacatgc cggagttcgt
3061 cgagcggccc gccgactact gggatgctgc cgacctgtat gaacgcgcca atgggcggct
3121 gttcaaggag gtcgaatttg ccctgccggt cgagctgacc ctcgaccagc agaaggcgct
3181 ggcgtccgag ttcgcccagc acctgaccgg tgccgagcgc ctgccgtata cgctggccat
3241 ccatgccggt ggcggcgaga acccgcactg ccacctgatg atctccgagc ggatcaatga
3301 cggcatcgag cggcccgccg ctcagtggtt caagcggtac aacggcaaga ccccggagaa
3361 gggcggggca cagaagaccg aagcgctcaa gcccaaggca tggcttgagc agacccgcga
3421 ggcatgggcc gaccatgcca accgggcatt agagcgggct ggccacgacg cccgcattga
3481 ccacagaaca cttgaggcgc agggcatcga gcgcctgccc ggtgttcacc tggggccgaa
3541 cgtggtggag atggaaggcc ggggcatccg caccgaccgg gcagacgtgg ccctgaacat
3601 cgacaccgcc aacgcccaga tcatcgactt acaggaatac cgggaggcaa tagaccatga
3661 acgcaatcga cagagtgaag aaatccagag gcatcaacga gttagcggag cagatcgaac
3721 cgctggccca gagcatggcg acactggccg acgaagcccg gcaggtcatg agccagacca
3781 agcaggccag cgaggcgcag gcggcggagt ggctgaaagc ccagcgccag acaggggcgg
3841 catgggtgga gctggccaaa gagttgcggg aggtagccgc cgaggtgagc agcgccgcgc
3901 agagcgcccg gagcgcgtcg cgggggtggc actggaagct atggctaacc gtgatgctgg
3961 cttccatgat gcctacggtg gtgctgctga tcgcatcgtt gctcttgctc gacctgacgc
4021 cactgacaac cgaggacggc tcgatctggc tgcgcttggt ggcccgatga agaacgacag
4081 gactttgcag gccataggcc gacagctcaa ggccatgggc tgtgagcgct tcgatatcgg
4141 cgtcagggac gcacccaccg gccagatgat gaaccgggaa tggtcagccg ccgaagtgct
4201 ccagaacacg ccatggctca agcggatgaa tgcccagggc aatgacgtgt atatcaggcc
4261 cgccgagcag gagcggcatg gtctggtgct ggtggacgac ctcagcgagt ttgacctgga
4321 tgacatgaaa gccgagggcc gggagcctgc cctggtagtg gaaaccagcc cgaagaacta
4381 tcaggcatgg gtcaaggtgg ccgacgccgc aggcggtgaa cttcgggggc agattgcccg
4441 gacgctggcc agcgagtacg acgccgaccc ggccagcgcc gacagccgcc actatggccg
4501 cttggcgggc ttcaccaacc gcaaggacaa gcacaccacc cgcgccggtt atcagccgtg
4561 ggtgctgctg cgtgaatcca agggcaagac cgccaccgct ggcccggcgc tggtgcagca
4621 ggctggccag cagatcgagc aggcccagcg gcagcaggag aaggcccgca ggctggccag
4681 cctcgaactg cccgagcggc agcttagccg ccaccggcgc acggcgctgg acgagtaccg
4741 cagcgagatg gccgggctgg tcaagcgctt cggtcatgac ctcagcaagt gcgactttat
4801 cgccgcgcag aagctggcca gccggggccg cagtgccgag gaaatcggca aggccatggc
4861 cgaggccagc ccagcgctgg cagagcgcaa gcccggccac gaagcggatt acatcgagcg
4921 caccgtcagc aaggtcatgg gtctgcccag cgtccagctt gcgcgggccg agctggcacg
4981 ggcaccggca ccccgccagc gaggcatgga caggggcggg ccagatttca gcatgtagtg
5041 cttgcgttgg tactcacgcc tgttatacta tgagtactca cgcacagaag ggggttttat
5101 ggaatacgaa aaaagcgctt cagggtcggt ctacctgatc aaaagtgaca agggctattg
5161 gttgcccggt ggctttggtt atacgtcaaa caaggccgag gctggccgct tttcagtcgc
5221 tgatatggcc agccttaacc ttgacggctg caccttgtcc ttgttccgcg aagacaagcc
5281 tttcggcccc ggcaagtttc tcggtgactg atatgaaaga ccaaaaggac aagcagaccg
5341 gcgacctgct ggccagccct gacgctgtac gccaagcgcg atatgccgag cgcatgaagg
5401 ccaaagggat gcgtcagcgc aagttctggc tgaccgacga cgaatacgag gcgctgcgcg
5461 agtgcctgga agaactcaga gcggcgcagg gcgggggtag tgaccccgcc agcgcctaac
5521 caccaactgc ctgcaaagga ggcaatcaat ggctacccat aagcctatca atattctgga
5581 ggcgttcgca gcagcgccgc caccgctgga ctacgttttg cccaacatgg tggccggtac
5641 ggtcggggcg ctggtgtcgc ccggtggtgc cggtaaatcc atgctggccc tgcaactggc
5701 cgcacagatt gcaggcgggc cggatctgct ggaggtgggc gaactgccca ccggcccggt
5761 gatctacctg cccgccgaag acccgcccac cgccattcat caccgcctgc acgcccttgg
5821 ggcgcacctc agcgccgagg aacggcaagc cgtggctgac ggcctgctga tccagccgct
5881 gatcggcagc ctgcccaaca tcatggcccc ggagtggttc gacggcctca agcgcgccgc
5941 cgagggccgc cgcctgatgg tgctggacac gctgcgccgg ttccacatcg aggaagaaaa
6001 cgccagcggc cccatggccc aggtcatcgg tcgcatggag gccatcgccg ccgataccgg
6061 gtgctctatc gtgttcctgc accatgccag caagggcgcg gccatgatgg gcgcaggcga
6121 ccagcagcag gccagccggg gcagctcggt actggtcgat aacatccgct ggcagtccta
6181 cctgtcgagc atgaccagcg ccgaggccga ggaatggggt gtggacgacg accagcgccg
6241 gttcttcgtc cgcttcggtg tgagcaaggc caactatggc gcaccgttcg ctgatcggtg
6301 gttcaggcgg catgacggcg gggtgctcaa gcccgccgtg ctggagaggc agcgcaagag
6361 caagggggtg ccccgtggtg aagcctaaga acaagcacag cctcagccac gtccggcacg
6421 acccggcgca ctgtctggcc cccggcctgt tccgtgccct caagcggggc gagcgcaagc
6481 gcagcaagct ggacgtgacg tatgactacg gcgacggcaa gcggatcgag ttcagcggcc
6541 cggagccgct gggcgctgat gatctgcgca tcctgcaagg gctggtggcc atggctgggc
6601 ctaatggcct agtgcttggc ccggaaccca agaccgaagg cggacggcag ctccggctgt
6661 tcctggaacc caagtgggag gccgtcaccg ctgaatgcca tgtggtcaaa ggtagctatc
6721 gggcgctggc aaaggaaatc ggggcagagg tcgatagtgg tggggcgctc aagcacatac
6781 aggactgcat cgagcgcctt tggaaggtat ccatcatcgc ccagaatggc cgcaagcggc
6841 aggggtttcg gctgctgtcg gagtacgcca gcgacgaggc ggacgggcgc ctgtacgtgg
6901 ccctgaaccc cttgatcgcg caggccgtca tgggtggcgg ccagcatgtg cgcatcagca
6961 tggacgaggt gcgggcgctg gacagcgaaa ccgcccgcct gctgcaccag cggctgtgtg
7021 gctggatcga ccccggcaaa accggcaagg cttccataga taccttgtgc ggctatgtct
7081 ggccgtcaga ggccagtggt tcgaccatgc gcaagcgccg caagcgggtg cgcgaggcgt
7141 tgccggagct ggtcgcgctg ggctggacgg taaccgagtt cgcggcgggc aagtacgaca
7201 tcacccggcc caaggcggca ggctgacccc ccccactcta ttgtaaacaa gacattttta
7261 tcttttatat tcaatggctt attttcctgc taattggtaa taccatgaaa aataccatgc
7321 tcagaaaagg cttaacaata ttttgaaaaa ttgcctactg agcgctgccg cacagctcca
7381 taggccgctt tcctggcttt gcttccagat gtatgctctt ctgctcccga acgccagcaa
7441 gacgtagccc agcgcgtcgg ccagcttgca attcgcgcta acttacatta attgcgttgc
7501 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
7561 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
7621 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
7681 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
7741 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc
7801 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
7861 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
7921 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
7981 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
8041 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg
8101 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
8161 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
8221 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
8281 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
8341 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
8401 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
8461 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
8521 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
8581 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
8641 gttttgcacc attcgatggt gtcaacgtaa atgccgcttc gccttcgcgc gcgaattgca
8701 agctgatccg ggcttatcga ctgcacggtg caccaatgct tctggcgtca ggctgccatc
8761 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
8821 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
8881 tgaaatgagc tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat
8941 aacaatttca cacaggaaac taggcacccc aggctttaca ctttatgctt ccggctcgta
9001 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
9061 acgaa
//GenBank-Updates@genbank.bio.net (03/20/91)
LOCUS PMMB190 9065 bp ds-DNA Circular SYN 20-MAR-1991
DEFINITION Bacterial cloning vector pMMB190, complete genome.
ACCESSION M37846
KEYWORDS DNA helicase; alpha peptide; beta-lactamase; lac repressor protein;
primase; regulatory protein; replication initiator.
SOURCE Bacterial cloning vector pMMB190.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (bases 1 to 9065)
AUTHORS Morales,V.M., Baeckman,A. and Bagdasarian,M.
TITLE A series of wide-host-range low-copy-number vectors that allow
direct screening for recombinants
JOURNAL Gene 97, 39-47 (1991)
STANDARD simple staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by V.M.Morales, 21-AUG-1990.
Natl Res Council, Canada
Plant Biotech Inst
Saskatoon, Sask.
S7N OW9 Canada
FEATURES Location/Qualifiers
CDS join(9047..9063,1..181)
/product="alpha peptide"
/gene="lacZ'"
/evidence=EXPERIMENTAL
/codon_start=9047
promoter 638..668
/gene="bla"
CDS 708..1568
/product="beta-lactamase"
/gene="bla"
/codon_start=708
rep_origin 2006..2400
CDS complement(2426..2710)
/function="conjugational DNA transfer"
/gene="mobC"
/codon_start=2708
misc_feature 2663..2820
/note="origin of conjugational DNA transfer"
promoter 2702..2732
/note="P1"
promoter 2721..2751
/note="P3"
CDS 2909..5038
/product="primase"
/gene="repB/mobA"
/codon_start=2909
CDS 3657..4070
/product="conjugational DNA transfer protein"
/gene="mobB"
/codon_start=3657
CDS 4067..5038
/product="primase"
/gene="repB'"
/codon_start=4067
promoter 5040..5071
/note="P4"
CDS 5099..5311
/note="protein of unknown function"
/gene="E"
/codon_start=5099
CDS 5313..5519
/note="regulates repA and repC gene expression."
/product="regulatory protein"
/gene="cac"
/codon_start=5313
CDS 5549..6388
/product="DNA helicase"
/gene="repA"
/codon_start=5549
CDS 6375..7226
/note="contains stop codons."
/product="replication initiator protein"
/gene="repC"
/codon_start=6375
promoter 7387..7417
/note="P5"
CDS complement(7503..8585)
/note="contains stop codons."
/product="lac repressor"
/gene="lacI"
/codon_start=8583
promoter 8620..8649
/gene="lacIq"
promoter 8893..8921
/gene="tac"
promoter 8975..9004
/gene="lacUV5"
BASE COUNT 1962 a 2654 c 2692 g 1757 t
ORIGIN
1 ttcccgggga tccgtcgacc tgcagccaag cttggcactg gccgtcgttt tacaacgtcg
61 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
121 cagctggcta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
181 aatggcgatg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
241 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
301 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
361 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
421 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
481 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
541 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
601 gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
661 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
721 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
781 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
841 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
901 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
961 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
1021 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
1081 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
1141 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
1201 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
1261 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
1321 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
1381 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
1441 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
1501 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
1561 attggtaact gtcagaccaa gtttactcat atatacttta gattgatttc tgaaagcgac
1621 caggtgctcg gcgtggcaag actcgcagcg aacccgtaga aagccatgct ccagccgccc
1681 gcattggaga aattcttcaa attcccgttg cacatagccc ggcaattcct ttccctgctc
1741 tgccataagc gcagcgaatg ccgggtaata ctcgtcaacg atctgataga gaagggtttg
1801 ctcgggtcgg tggctctggt aacgaccagt atcccgatcc cggctggccg tcctggccgc
1861 cacatgaggc atgttccgcg tccttgcaat actgtgttta catacagtct atcgcttagc
1921 ggaaagttct tttaccctca gccgaaatgc ctgccgttgc tagacattgc cagccagtgc
1981 ccgtcactcc cgtactaact gtcacgaacc cctgcaataa ctgtcacgcc cccctgcaat
2041 aactgtcacg aacccctgca ataactgtca cgcccccaaa cctgcaaacc cagcaggggc
2101 gggggctggc ggggtgttgg aaaaatccat ccatgattat ctaagaataa tccactaggc
2161 gcggttatca gcgcccttgt ggggcgctgc tgcccttgcc caatatgccc ggccagaggc
2221 cggatagctg gtctattcgc tgcgctaggc tacacaccgc cccaccgctg cgcggcaggg
2281 ggaaaggcgg gcaaagcccg ctaaacccca caccaaaccc cgcagaaata cgctggagcg
2341 cttttagccg ctttagcggc ctttccccct acccgaaggg tgggggcgcg tgtgcagccc
2401 cgcagggcct gtctcggtcg atcattcagc ccggctcatc cttctggcgt ggcggcagac
2461 cgaacaaggc gcggtcgtgg tcgcgttcaa ggtacgcatc cattgccgcc atgagccgat
2521 cctccggcca ctcgctgctg ttcaccttgg ccaaaatcat ggcccccacc agcaccttgc
2581 gccttgtttc gttcttgcgc tcttgctgct gttcccttgc ccgctcccgc tgaatttcgg
2641 cattgattcg cgctcgttgt tcttcgagct tggccagccg atccgccgcc ttgttgctcc
2701 ccttaaccat cttgacaccc cattgttaat gtgctgtctc gtaggctatc atggaggcac
2761 agcggcggca atcccgaccc tactttgtag gggagggcgc acttaccggt ttctcttcga
2821 gaaactggcc taacggccac ccttcgggcg gtgcgctctc cgagggccat tgcatggagc
2881 cgaaaagcaa aagcaacagc gaggcagcat ggcgatttat caccttacgg cgaaaaccgg
2941 cagcaggtcg ggcggccaat cggccagggc caaggccgac tacatccagc gcgaaggcaa
3001 gtatgcccgc gacatggatg aagtcttgca cgccgaatcc gggcacatgc cggagttcgt
3061 cgagcggccc gccgactact gggatgctgc cgacctgtat gaacgcgcca atgggcggct
3121 gttcaaggag gtcgaatttg ccctgccggt cgagctgacc ctcgaccagc agaaggcgct
3181 ggcgtccgag ttcgcccagc acctgaccgg tgccgagcgc ctgccgtata cgctggccat
3241 ccatgccggt ggcggcgaga acccgcactg ccacctgatg atctccgagc ggatcaatga
3301 cggcatcgag cggcccgccg ctcagtggtt caagcggtac aacggcaaga ccccggagaa
3361 gggcggggca cagaagaccg aagcgctcaa gcccaaggca tggcttgagc agacccgcga
3421 ggcatgggcc gaccatgcca accgggcatt agagcgggct ggccacgacg cccgcattga
3481 ccacagaaca cttgaggcgc agggcatcga gcgcctgccc ggtgttcacc tggggccgaa
3541 cgtggtggag atggaaggcc ggggcatccg caccgaccgg gcagacgtgg ccctgaacat
3601 cgacaccgcc aacgcccaga tcatcgactt acaggaatac cgggaggcaa tagaccatga
3661 acgcaatcga cagagtgaag aaatccagag gcatcaacga gttagcggag cagatcgaac
3721 cgctggccca gagcatggcg acactggccg acgaagcccg gcaggtcatg agccagacca
3781 agcaggccag cgaggcgcag gcggcggagt ggctgaaagc ccagcgccag acaggggcgg
3841 catgggtgga gctggccaaa gagttgcggg aggtagccgc cgaggtgagc agcgccgcgc
3901 agagcgcccg gagcgcgtcg cgggggtggc actggaagct atggctaacc gtgatgctgg
3961 cttccatgat gcctacggtg gtgctgctga tcgcatcgtt gctcttgctc gacctgacgc
4021 cactgacaac cgaggacggc tcgatctggc tgcgcttggt ggcccgatga agaacgacag
4081 gactttgcag gccataggcc gacagctcaa ggccatgggc tgtgagcgct tcgatatcgg
4141 cgtcagggac gcacccaccg gccagatgat gaaccgggaa tggtcagccg ccgaagtgct
4201 ccagaacacg ccatggctca agcggatgaa tgcccagggc aatgacgtgt atatcaggcc
4261 cgccgagcag gagcggcatg gtctggtgct ggtggacgac ctcagcgagt ttgacctgga
4321 tgacatgaaa gccgagggcc gggagcctgc cctggtagtg gaaaccagcc cgaagaacta
4381 tcaggcatgg gtcaaggtgg ccgacgccgc aggcggtgaa cttcgggggc agattgcccg
4441 gacgctggcc agcgagtacg acgccgaccc ggccagcgcc gacagccgcc actatggccg
4501 cttggcgggc ttcaccaacc gcaaggacaa gcacaccacc cgcgccggtt atcagccgtg
4561 ggtgctgctg cgtgaatcca agggcaagac cgccaccgct ggcccggcgc tggtgcagca
4621 ggctggccag cagatcgagc aggcccagcg gcagcaggag aaggcccgca ggctggccag
4681 cctcgaactg cccgagcggc agcttagccg ccaccggcgc acggcgctgg acgagtaccg
4741 cagcgagatg gccgggctgg tcaagcgctt cggtcatgac ctcagcaagt gcgactttat
4801 cgccgcgcag aagctggcca gccggggccg cagtgccgag gaaatcggca aggccatggc
4861 cgaggccagc ccagcgctgg cagagcgcaa gcccggccac gaagcggatt acatcgagcg
4921 caccgtcagc aaggtcatgg gtctgcccag cgtccagctt gcgcgggccg agctggcacg
4981 ggcaccggca ccccgccagc gaggcatgga caggggcggg ccagatttca gcatgtagtg
5041 cttgcgttgg tactcacgcc tgttatacta tgagtactca cgcacagaag ggggttttat
5101 ggaatacgaa aaaagcgctt cagggtcggt ctacctgatc aaaagtgaca agggctattg
5161 gttgcccggt ggctttggtt atacgtcaaa caaggccgag gctggccgct tttcagtcgc
5221 tgatatggcc agccttaacc ttgacggctg caccttgtcc ttgttccgcg aagacaagcc
5281 tttcggcccc ggcaagtttc tcggtgactg atatgaaaga ccaaaaggac aagcagaccg
5341 gcgacctgct ggccagccct gacgctgtac gccaagcgcg atatgccgag cgcatgaagg
5401 ccaaagggat gcgtcagcgc aagttctggc tgaccgacga cgaatacgag gcgctgcgcg
5461 agtgcctgga agaactcaga gcggcgcagg gcgggggtag tgaccccgcc agcgcctaac
5521 caccaactgc ctgcaaagga ggcaatcaat ggctacccat aagcctatca atattctgga
5581 ggcgttcgca gcagcgccgc caccgctgga ctacgttttg cccaacatgg tggccggtac
5641 ggtcggggcg ctggtgtcgc ccggtggtgc cggtaaatcc atgctggccc tgcaactggc
5701 cgcacagatt gcaggcgggc cggatctgct ggaggtgggc gaactgccca ccggcccggt
5761 gatctacctg cccgccgaag acccgcccac cgccattcat caccgcctgc acgcccttgg
5821 ggcgcacctc agcgccgagg aacggcaagc cgtggctgac ggcctgctga tccagccgct
5881 gatcggcagc ctgcccaaca tcatggcccc ggagtggttc gacggcctca agcgcgccgc
5941 cgagggccgc cgcctgatgg tgctggacac gctgcgccgg ttccacatcg aggaagaaaa
6001 cgccagcggc cccatggccc aggtcatcgg tcgcatggag gccatcgccg ccgataccgg
6061 gtgctctatc gtgttcctgc accatgccag caagggcgcg gccatgatgg gcgcaggcga
6121 ccagcagcag gccagccggg gcagctcggt actggtcgat aacatccgct ggcagtccta
6181 cctgtcgagc atgaccagcg ccgaggccga ggaatggggt gtggacgacg accagcgccg
6241 gttcttcgtc cgcttcggtg tgagcaaggc caactatggc gcaccgttcg ctgatcggtg
6301 gttcaggcgg catgacggcg gggtgctcaa gcccgccgtg ctggagaggc agcgcaagag
6361 caagggggtg ccccgtggtg aagcctaaga acaagcacag cctcagccac gtccggcacg
6421 acccggcgca ctgtctggcc cccggcctgt tccgtgccct caagcggggc gagcgcaagc
6481 gcagcaagct ggacgtgacg tatgactacg gcgacggcaa gcggatcgag ttcagcggcc
6541 cggagccgct gggcgctgat gatctgcgca tcctgcaagg gctggtggcc atggctgggc
6601 ctaatggcct agtgcttggc ccggaaccca agaccgaagg cggacggcag ctccggctgt
6661 tcctggaacc caagtgggag gccgtcaccg ctgaatgcca tgtggtcaaa ggtagctatc
6721 gggcgctggc aaaggaaatc ggggcagagg tcgatagtgg tggggcgctc aagcacatac
6781 aggactgcat cgagcgcctt tggaaggtat ccatcatcgc ccagaatggc cgcaagcggc
6841 aggggtttcg gctgctgtcg gagtacgcca gcgacgaggc ggacgggcgc ctgtacgtgg
6901 ccctgaaccc cttgatcgcg caggccgtca tgggtggcgg ccagcatgtg cgcatcagca
6961 tggacgaggt gcgggcgctg gacagcgaaa ccgcccgcct gctgcaccag cggctgtgtg
7021 gctggatcga ccccggcaaa accggcaagg cttccataga taccttgtgc ggctatgtct
7081 ggccgtcaga ggccagtggt tcgaccatgc gcaagcgccg caagcgggtg cgcgaggcgt
7141 tgccggagct ggtcgcgctg ggctggacgg taaccgagtt cgcggcgggc aagtacgaca
7201 tcacccggcc caaggcggca ggctgacccc ccccactcta ttgtaaacaa gacattttta
7261 tcttttatat tcaatggctt attttcctgc taattggtaa taccatgaaa aataccatgc
7321 tcagaaaagg cttaacaata ttttgaaaaa ttgcctactg agcgctgccg cacagctcca
7381 taggccgctt tcctggcttt gcttccagat gtatgctctt ctgctcccga acgccagcaa
7441 gacgtagccc agcgcgtcgg ccagcttgca attcgcgcta acttacatta attgcgttgc
7501 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
7561 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
7621 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
7681 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
7741 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc
7801 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
7861 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
7921 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
7981 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
8041 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg
8101 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
8161 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
8221 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
8281 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
8341 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
8401 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
8461 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
8521 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
8581 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
8641 gttttgcacc attcgatggt gtcaacgtaa atgccgcttc gccttcgcgc gcgaattgca
8701 agctgatccg ggcttatcga ctgcacggtg caccaatgct tctggcgtca ggctgccatc
8761 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc
8821 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc
8881 tgaaatgagc tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat
8941 aacaatttca cacaggaaac taggcacccc aggctttaca ctttatgctt ccggctcgta
9001 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
9061 acgaa
//