GenBank-Updates@genbank.bio.net (01/08/91)
LOCUS PMMB206 9312 bp Circular SYN 08-JAN-1991
DEFINITION Bacterial cloning vector pMMB206, complete genome.
ACCESSION M37847
KEYWORDS alpha peptide; beta-lactamase; chloramphenicol acetyl transferase;
conjugational DNA transfer protein; helicase; lac repressor;
primase; regulatory protein; replication initiation protein.
SOURCE Cloning vector pMMB206 DNA.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (bases 1 to 9312)
AUTHORS Morales,V.M., Backman,A. and Bagdasarian,M.
TITLE A series of broad-host-range low-copy-number cloning vectors that
allow direct screening for recombinants
JOURNAL Unpublished (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by V.M.Morales, 21-AUG-1990.
Natl Res Council, Canada
Plant Biotech Inst
Saskatoon, Sask.
S7N OW9 Canada
FEATURES Location/Qualifiers
CDS join(9295..9311,1..181)
/gene="lacZ'"
/product="alfa peptide"
promoter 638..668
/gene="bla"
CDS 708..941
/note="truncated"
/gene="bla"
/codon_start=708
/product="beta-lactamase"
promoter 1168..1194
/gene="cat"
CDS 1261..1920
/gene="cat"
/codon_start=1261
/product="chloramphenicol acetyl transferase"
rep_origin 2234..2647
CDS complement(2673..2957)
/gene="mobC"
/codon_start=2673
/product="conjugational DNA transfer protein"
misc_feature 2910..3067
/note="origin of conjugational DNA transfer"
promoter 2968..2998
/note="P3"
promoter 3019..2990
/note="P2"
CDS 3156..5285
/gene="repB/mobA"
/codon_start=3156
/product="primase"
CDS 3904..4317
/gene="mobB"
/codon_start=3904
/product="conjugational DNA transfer protein"
CDS 4314..5285
/gene="repB'"
/codon_start=4314
/product="primase"
promoter 5287..5318
/note="P4"
CDS 5346..5558
/note="protein of unknown function"
/gene="E"
/codon_start=5346
CDS 5560..5766
/note="Regulates repC and repA gene expression."
/gene="cac"
/codon_start=5560
/product="regulatory protein"
CDS 5796..6635
/gene="repA"
/codon_start=5796
/product="DNA helicase"
CDS 6622..7473
/gene="repC"
/codon_start=6622
/product="replication initiator protein"
promoter 7634..7664
/note="P5"
CDS complement(7750..8832)
/note="stop codons in coding region."
/gene="lacI"
/codon_start=7750
/product="lac repressor"
promoter 8896..8867
/gene="lacIq"
promoter 9140..9168
/gene="tac"
promoter 9223..9251
/gene="lacUV5"
BASE COUNT 2042 a 2693 c 2726 g 1851 t
ORIGIN
1 ttcccgggga tccgtcgacc tgcagccaag cttggcactg gccgtcgttt tacaacgtcg
61 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
121 cagctggcta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
181 aatggcgatg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
241 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
301 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
361 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
421 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
481 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
541 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
601 gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
661 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
721 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
781 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
841 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgtgatg
901 tccggcggtg cttttgccgt tacgcaccac cccgtcagta gctgaacagg agggacagct
961 gatagaaaca gaagccactg gagcaagcac ctcaaaaaca ccatcataca ctaaatcagt
1021 aagttggcag catcacccga cgcactttgc gccgaataaa tacctgtgac ggaagatcac
1081 ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt
1141 tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata
1201 agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa
1261 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
1321 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
1381 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
1441 cacattcttg cccgcctgat gaatgctcat ccggaattac gtatggcaat gaaagacggt
1501 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
1561 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
1621 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
1681 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
1741 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
1801 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
1861 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
1921 tttttttaag gcagttattg tcttcaaatt cccgttgcac atagcccggc aattcctttc
1981 cctgctctgc cataagcgca gcgaatgccg ggtaatactc gtcaacgatc tgatagagaa
2041 gggtttgctc gggtcggtgg ctctggtaac gaccagtatc ccgatcccgg ctggccgtcc
2101 tggccgccac atgaggcatg ttccgcgtcc ttgcaatact gtgtttacat acagtctatc
2161 gcttagcgga aagttctttt accctcagcc gaaatgcctg ccgttgctag acattgccag
2221 ccagtgcccg tcactcccgt actaactgtc acgaacccct gcaataactg tcacgccccc
2281 ctgcaataac tgtcacgaac ccctgcaata actgtcacgc ccccaaacct gcaaacccag
2341 caggggcggg ggctggcggg gtgttggaaa aatccatcca tgattatcta agaataatcc
2401 actaggcgcg gttatcagcg cccttgtggg gcgctgctgc ccttgcccaa tatgcccggc
2461 cagaggccgg atagctggtc tattcgctgc gctaggctac acaccgcccc accgctgcgc
2521 ggcaggggga aaggcgggca aagcccgcta aaccccacac caaaccccgc agaaatacgc
2581 tggagcgctt ttagccgctt tagcggcctt tccccctacc cgaagggtgg gggcgcgtgt
2641 gcagccccgc agggcctgtc tcggtcgatc attcagcccg gctcatcctt ctggcgtggc
2701 ggcagaccga acaaggcgcg gtcgtggtcg cgttcaaggt acgcatccat tgccgccatg
2761 agccgatcct ccggccactc gctgctgttc accttggcca aaatcatggc ccccaccagc
2821 accttgcgcc ttgtttcgtt cttgcgctct tgctgctgtt cccttgcccg ctcccgctga
2881 atttcggcat tgattcgcgc tcgttgttct tcgagcttgg ccagccgatc cgccgccttg
2941 ttgctcccct taaccatctt gacaccccat tgttaatgtg ctgtctcgta ggctatcatg
3001 gaggcacagc ggcggcaatc ccgaccctac tttgtagggg agggcgcact taccggtttc
3061 tcttcgagaa actggcctaa cggccaccct tcgggcggtg cgctctccga gggccattgc
3121 atggagccga aaagcaaaag caacagcgag gcagcatggc gatttatcac cttacggcga
3181 aaaccggcag caggtcgggc ggccaatcgg ccagggccaa ggccgactac atccagcgcg
3241 aaggcaagta tgcccgcgac atggatgaag tcttgcacgc cgaatccggg cacatgccgg
3301 agttcgtcga gcggcccgcc gactactggg atgctgccga cctgtatgaa cgcgccaatg
3361 ggcggctgtt caaggaggtc gaatttgccc tgccggtcga gctgaccctc gaccagcaga
3421 aggcgctggc gtccgagttc gcccagcacc tgaccggtgc cgagcgcctg ccgtatacgc
3481 tggccatcca tgccggtggc ggcgagaacc cgcactgcca cctgatgatc tccgagcgga
3541 tcaatgacgg catcgagcgg cccgccgctc agtggttcaa gcggtacaac ggcaagaccc
3601 cggagaaggg cggggcacag aagaccgaag cgctcaagcc caaggcatgg cttgagcaga
3661 cccgcgaggc atgggccgac catgccaacc gggcattaga gcgggctggc cacgacgccc
3721 gcattgacca cagaacactt gaggcgcagg gcatcgagcg cctgcccggt gttcacctgg
3781 ggccgaacgt ggtggagatg gaaggccggg gcatccgcac cgaccgggca gacgtggccc
3841 tgaacatcga caccgccaac gcccagatca tcgacttaca ggaataccgg gaggcaatag
3901 accatgaacg caatcgacag agtgaagaaa tccagaggca tcaacgagtt agcggagcag
3961 atcgaaccgc tggcccagag catggcgaca ctggccgacg aagcccggca ggtcatgagc
4021 cagaccaagc aggccagcga ggcgcaggcg gcggagtggc tgaaagccca gcgccagaca
4081 ggggcggcat gggtggagct ggccaaagag ttgcgggagg tagccgccga ggtgagcagc
4141 gccgcgcaga gcgcccggag cgcgtcgcgg gggtggcact ggaagctatg gctaaccgtg
4201 atgctggctt ccatgatgcc tacggtggtg ctgctgatcg catcgttgct cttgctcgac
4261 ctgacgccac tgacaaccga ggacggctcg atctggctgc gcttggtggc ccgatgaaga
4321 acgacaggac tttgcaggcc ataggccgac agctcaaggc catgggctgt gagcgcttcg
4381 atatcggcgt cagggacgca cccaccggcc agatgatgaa ccgggaatgg tcagccgccg
4441 aagtgctcca gaacacgcca tggctcaagc ggatgaatgc ccagggcaat gacgtgtata
4501 tcaggcccgc cgagcaggag cggcatggtc tggtgctggt ggacgacctc agcgagtttg
4561 acctggatga catgaaagcc gagggccggg agcctgccct ggtagtggaa accagcccga
4621 agaactatca ggcatgggtc aaggtggccg acgccgcagg cggtgaactt cgggggcaga
4681 ttgcccggac gctggccagc gagtacgacg ccgacccggc cagcgccgac agccgccact
4741 atggccgctt ggcgggcttc accaaccgca aggacaagca caccacccgc gccggttatc
4801 agccgtgggt gctgctgcgt gaatccaagg gcaagaccgc caccgctggc ccggcgctgg
4861 tgcagcaggc tggccagcag atcgagcagg cccagcggca gcaggagaag gcccgcaggc
4921 tggccagcct cgaactgccc gagcggcagc ttagccgcca ccggcgcacg gcgctggacg
4981 agtaccgcag cgagatggcc gggctggtca agcgcttcgg tcatgacctc agcaagtgcg
5041 actttatcgc cgcgcagaag ctggccagcc ggggccgcag tgccgaggaa atcggcaagg
5101 ccatggccga ggccagccca gcgctggcag agcgcaagcc cggccacgaa gcggattaca
5161 tcgagcgcac cgtcagcaag gtcatgggtc tgcccagcgt ccagcttgcg cgggccgagc
5221 tggcacgggc accggcaccc cgccagcgag gcatggacag gggcgggcca gatttcagca
5281 tgtagtgctt gcgttggtac tcacgcctgt tatactatga gtactcacgc acagaagggg
5341 gttttatgga atacgaaaaa agcgcttcag ggtcggtcta cctgatcaaa agtgacaagg
5401 gctattggtt gcccggtggc tttggttata cgtcaaacaa ggccgaggct ggccgctttt
5461 cagtcgctga tatggccagc cttaaccttg acggctgcac cttgtccttg ttccgcgaag
5521 acaagccttt cggccccggc aagtttctcg gtgactgata tgaaagacca aaaggacaag
5581 cagaccggcg acctgctggc cagccctgac gctgtacgcc aagcgcgata tgccgagcgc
5641 atgaaggcca aagggatgcg tcagcgcaag ttctggctga ccgacgacga atacgaggcg
5701 ctgcgcgagt gcctggaaga actcagagcg gcgcagggcg ggggtagtga ccccgccagc
5761 gcctaaccac caactgcctg caaaggaggc aatcaatggc tacccataag cctatcaata
5821 ttctggaggc gttcgcagca gcgccgccac cgctggacta cgttttgccc aacatggtgg
5881 ccggtacggt cggggcgctg gtgtcgcccg gtggtgccgg taaatccatg ctggccctgc
5941 aactggccgc acagattgca ggcgggccgg atctgctgga ggtgggcgaa ctgcccaccg
6001 gcccggtgat ctacctgccc gccgaagacc cgcccaccgc cattcatcac cgcctgcacg
6061 cccttggggc gcacctcagc gccgaggaac ggcaagccgt ggctgacggc ctgctgatcc
6121 agccgctgat cggcagcctg cccaacatca tggccccgga gtggttcgac ggcctcaagc
6181 gcgccgccga gggccgccgc ctgatggtgc tggacacgct gcgccggttc cacatcgagg
6241 aagaaaacgc cagcggcccc atggcccagg tcatcggtcg catggaggcc atcgccgccg
6301 ataccgggtg ctctatcgtg ttcctgcacc atgccagcaa gggcgcggcc atgatgggcg
6361 caggcgacca gcagcaggcc agccggggca gctcggtact ggtcgataac atccgctggc
6421 agtcctacct gtcgagcatg accagcgccg aggccgagga atggggtgtg gacgacgacc
6481 agcgccggtt cttcgtccgc ttcggtgtga gcaaggccaa ctatggcgca ccgttcgctg
6541 atcggtggtt caggcggcat gacggcgggg tgctcaagcc cgccgtgctg gagaggcagc
6601 gcaagagcaa gggggtgccc cgtggtgaag cctaagaaca agcacagcct cagccacgtc
6661 cggcacgacc cggcgcactg tctggccccc ggcctgttcc gtgccctcaa gcggggcgag
6721 cgcaagcgca gcaagctgga cgtgacgtat gactacggcg acggcaagcg gatcgagttc
6781 agcggcccgg agccgctggg cgctgatgat ctgcgcatcc tgcaagggct ggtggccatg
6841 gctgggccta atggcctagt gcttggcccg gaacccaaga ccgaaggcgg acggcagctc
6901 cggctgttcc tggaacccaa gtgggaggcc gtcaccgctg aatgccatgt ggtcaaaggt
6961 agctatcggg cgctggcaaa ggaaatcggg gcagaggtcg atagtggtgg ggcgctcaag
7021 cacatacagg actgcatcga gcgcctttgg aaggtatcca tcatcgccca gaatggccgc
7081 aagcggcagg ggtttcggct gctgtcggag tacgccagcg acgaggcgga cgggcgcctg
7141 tacgtggccc tgaacccctt gatcgcgcag gccgtcatgg gtggcggcca gcatgtgcgc
7201 atcagcatgg acgaggtgcg ggcgctggac agcgaaaccg cccgcctgct gcaccagcgg
7261 ctgtgtggct ggatcgaccc cggcaaaacc ggcaaggctt ccatagatac cttgtgcggc
7321 tatgtctggc cgtcagaggc cagtggttcg accatgcgca agcgccgcaa gcgggtgcgc
7381 gaggcgttgc cggagctggt cgcgctgggc tggacggtaa ccgagttcgc ggcgggcaag
7441 tacgacatca cccggcccaa ggcggcaggc tgaccccccc cactctattg taaacaagac
7501 atttttatct tttatattca atggcttatt ttcctgctaa ttggtaatac catgaaaaat
7561 accatgctca gaaaaggctt aacaatattt tgaaaaattg cctactgagc gctgccgcac
7621 agctccatag gccgctttcc tggctttgct tccagatgta tgctcttctg ctcccgaacg
7681 ccagcaagac gtagcccagc gcgtcggcca gcttgcaatt cgcgctaact tacattaatt
7741 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
7801 atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt
7861 caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag
7921 caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg
7981 cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc
8041 aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc
8101 aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc
8161 ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag
8221 atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa
8281 cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc
8341 ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc
8401 cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt
8461 aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc
8521 gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga
8581 tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc
8641 aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag
8701 ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt
8761 caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt
8821 tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc
8881 gcgaaaggtt ttgcaccatt cgatggtgtc aacgtaaatg ccgcttcgcc ttcgcgcgcg
8941 aattgcaagc tgatccgggc ttatcgactg cacggtgcac caatgcttct ggcgtcaggc
9001 agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc
9061 tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca
9121 aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg
9181 agcggataac aatttcacac aggaaactag gcaccccagg ctttacactt tatgcttccg
9241 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
9301 catgattacg aa
//GenBank-Updates@genbank.bio.net (01/15/91)
LOCUS PMMB206 9312 bp Circular SYN 14-JAN-1991
DEFINITION Bacterial cloning vector pMMB206, complete genome.
ACCESSION M37847
KEYWORDS alpha peptide; beta-lactamase; chloramphenicol acetyl transferase;
conjugational DNA transfer protein; helicase; lac repressor;
primase; regulatory protein; replication initiation protein.
SOURCE Cloning vector pMMB206 DNA.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (bases 1 to 9312)
AUTHORS Morales,V.M., Backman,A. and Bagdasarian,M.
TITLE A series of broad-host-range low-copy-number cloning vectors that
allow direct screening for recombinants
JOURNAL Unpublished (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by V.M.Morales, 21-AUG-1990.
Natl Res Council, Canada
Plant Biotech Inst
Saskatoon, Sask.
S7N OW9 Canada
FEATURES Location/Qualifiers
CDS join(9295..9311,1..181)
/gene="lacZ'"
/product="alfa peptide"
promoter 638..668
/gene="bla"
CDS 708..941
/note="truncated"
/gene="bla"
/codon_start=708
/product="beta-lactamase"
promoter 1168..1194
/gene="cat"
CDS 1261..1920
/gene="cat"
/codon_start=1261
/product="chloramphenicol acetyl transferase"
rep_origin 2234..2647
CDS complement(2673..2957)
/gene="mobC"
/codon_start=2673
/product="conjugational DNA transfer protein"
misc_feature 2910..3067
/note="origin of conjugational DNA transfer"
promoter 2968..2998
/note="P3"
promoter 3019..2990
/note="P2"
CDS 3156..5285
/gene="repB/mobA"
/codon_start=3156
/product="primase"
CDS 3904..4317
/gene="mobB"
/codon_start=3904
/product="conjugational DNA transfer protein"
CDS 4314..5285
/gene="repB'"
/codon_start=4314
/product="primase"
promoter 5287..5318
/note="P4"
CDS 5346..5558
/note="protein of unknown function"
/gene="E"
/codon_start=5346
CDS 5560..5766
/note="Regulates repC and repA gene expression."
/gene="cac"
/codon_start=5560
/product="regulatory protein"
CDS 5796..6635
/gene="repA"
/codon_start=5796
/product="DNA helicase"
CDS 6622..7473
/gene="repC"
/codon_start=6622
/product="replication initiator protein"
promoter 7634..7664
/note="P5"
CDS complement(7750..8832)
/note="stop codons in coding region."
/gene="lacI"
/codon_start=7750
/product="lac repressor"
promoter 8896..8867
/gene="lacIq"
promoter 9140..9168
/gene="tac"
promoter 9223..9251
/gene="lacUV5"
BASE COUNT 2042 a 2693 c 2726 g 1851 t
ORIGIN
1 ttcccgggga tccgtcgacc tgcagccaag cttggcactg gccgtcgttt tacaacgtcg
61 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
121 cagctggcta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
181 aatggcgatg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
241 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
301 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
361 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
421 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
481 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
541 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
601 gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
661 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
721 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
781 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
841 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgtgatg
901 tccggcggtg cttttgccgt tacgcaccac cccgtcagta gctgaacagg agggacagct
961 gatagaaaca gaagccactg gagcaagcac ctcaaaaaca ccatcataca ctaaatcagt
1021 aagttggcag catcacccga cgcactttgc gccgaataaa tacctgtgac ggaagatcac
1081 ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt
1141 tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata
1201 agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa
1261 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
1321 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
1381 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
1441 cacattcttg cccgcctgat gaatgctcat ccggaattac gtatggcaat gaaagacggt
1501 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
1561 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
1621 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
1681 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
1741 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
1801 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
1861 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
1921 tttttttaag gcagttattg tcttcaaatt cccgttgcac atagcccggc aattcctttc
1981 cctgctctgc cataagcgca gcgaatgccg ggtaatactc gtcaacgatc tgatagagaa
2041 gggtttgctc gggtcggtgg ctctggtaac gaccagtatc ccgatcccgg ctggccgtcc
2101 tggccgccac atgaggcatg ttccgcgtcc ttgcaatact gtgtttacat acagtctatc
2161 gcttagcgga aagttctttt accctcagcc gaaatgcctg ccgttgctag acattgccag
2221 ccagtgcccg tcactcccgt actaactgtc acgaacccct gcaataactg tcacgccccc
2281 ctgcaataac tgtcacgaac ccctgcaata actgtcacgc ccccaaacct gcaaacccag
2341 caggggcggg ggctggcggg gtgttggaaa aatccatcca tgattatcta agaataatcc
2401 actaggcgcg gttatcagcg cccttgtggg gcgctgctgc ccttgcccaa tatgcccggc
2461 cagaggccgg atagctggtc tattcgctgc gctaggctac acaccgcccc accgctgcgc
2521 ggcaggggga aaggcgggca aagcccgcta aaccccacac caaaccccgc agaaatacgc
2581 tggagcgctt ttagccgctt tagcggcctt tccccctacc cgaagggtgg gggcgcgtgt
2641 gcagccccgc agggcctgtc tcggtcgatc attcagcccg gctcatcctt ctggcgtggc
2701 ggcagaccga acaaggcgcg gtcgtggtcg cgttcaaggt acgcatccat tgccgccatg
2761 agccgatcct ccggccactc gctgctgttc accttggcca aaatcatggc ccccaccagc
2821 accttgcgcc ttgtttcgtt cttgcgctct tgctgctgtt cccttgcccg ctcccgctga
2881 atttcggcat tgattcgcgc tcgttgttct tcgagcttgg ccagccgatc cgccgccttg
2941 ttgctcccct taaccatctt gacaccccat tgttaatgtg ctgtctcgta ggctatcatg
3001 gaggcacagc ggcggcaatc ccgaccctac tttgtagggg agggcgcact taccggtttc
3061 tcttcgagaa actggcctaa cggccaccct tcgggcggtg cgctctccga gggccattgc
3121 atggagccga aaagcaaaag caacagcgag gcagcatggc gatttatcac cttacggcga
3181 aaaccggcag caggtcgggc ggccaatcgg ccagggccaa ggccgactac atccagcgcg
3241 aaggcaagta tgcccgcgac atggatgaag tcttgcacgc cgaatccggg cacatgccgg
3301 agttcgtcga gcggcccgcc gactactggg atgctgccga cctgtatgaa cgcgccaatg
3361 ggcggctgtt caaggaggtc gaatttgccc tgccggtcga gctgaccctc gaccagcaga
3421 aggcgctggc gtccgagttc gcccagcacc tgaccggtgc cgagcgcctg ccgtatacgc
3481 tggccatcca tgccggtggc ggcgagaacc cgcactgcca cctgatgatc tccgagcgga
3541 tcaatgacgg catcgagcgg cccgccgctc agtggttcaa gcggtacaac ggcaagaccc
3601 cggagaaggg cggggcacag aagaccgaag cgctcaagcc caaggcatgg cttgagcaga
3661 cccgcgaggc atgggccgac catgccaacc gggcattaga gcgggctggc cacgacgccc
3721 gcattgacca cagaacactt gaggcgcagg gcatcgagcg cctgcccggt gttcacctgg
3781 ggccgaacgt ggtggagatg gaaggccggg gcatccgcac cgaccgggca gacgtggccc
3841 tgaacatcga caccgccaac gcccagatca tcgacttaca ggaataccgg gaggcaatag
3901 accatgaacg caatcgacag agtgaagaaa tccagaggca tcaacgagtt agcggagcag
3961 atcgaaccgc tggcccagag catggcgaca ctggccgacg aagcccggca ggtcatgagc
4021 cagaccaagc aggccagcga ggcgcaggcg gcggagtggc tgaaagccca gcgccagaca
4081 ggggcggcat gggtggagct ggccaaagag ttgcgggagg tagccgccga ggtgagcagc
4141 gccgcgcaga gcgcccggag cgcgtcgcgg gggtggcact ggaagctatg gctaaccgtg
4201 atgctggctt ccatgatgcc tacggtggtg ctgctgatcg catcgttgct cttgctcgac
4261 ctgacgccac tgacaaccga ggacggctcg atctggctgc gcttggtggc ccgatgaaga
4321 acgacaggac tttgcaggcc ataggccgac agctcaaggc catgggctgt gagcgcttcg
4381 atatcggcgt cagggacgca cccaccggcc agatgatgaa ccgggaatgg tcagccgccg
4441 aagtgctcca gaacacgcca tggctcaagc ggatgaatgc ccagggcaat gacgtgtata
4501 tcaggcccgc cgagcaggag cggcatggtc tggtgctggt ggacgacctc agcgagtttg
4561 acctggatga catgaaagcc gagggccggg agcctgccct ggtagtggaa accagcccga
4621 agaactatca ggcatgggtc aaggtggccg acgccgcagg cggtgaactt cgggggcaga
4681 ttgcccggac gctggccagc gagtacgacg ccgacccggc cagcgccgac agccgccact
4741 atggccgctt ggcgggcttc accaaccgca aggacaagca caccacccgc gccggttatc
4801 agccgtgggt gctgctgcgt gaatccaagg gcaagaccgc caccgctggc ccggcgctgg
4861 tgcagcaggc tggccagcag atcgagcagg cccagcggca gcaggagaag gcccgcaggc
4921 tggccagcct cgaactgccc gagcggcagc ttagccgcca ccggcgcacg gcgctggacg
4981 agtaccgcag cgagatggcc gggctggtca agcgcttcgg tcatgacctc agcaagtgcg
5041 actttatcgc cgcgcagaag ctggccagcc ggggccgcag tgccgaggaa atcggcaagg
5101 ccatggccga ggccagccca gcgctggcag agcgcaagcc cggccacgaa gcggattaca
5161 tcgagcgcac cgtcagcaag gtcatgggtc tgcccagcgt ccagcttgcg cgggccgagc
5221 tggcacgggc accggcaccc cgccagcgag gcatggacag gggcgggcca gatttcagca
5281 tgtagtgctt gcgttggtac tcacgcctgt tatactatga gtactcacgc acagaagggg
5341 gttttatgga atacgaaaaa agcgcttcag ggtcggtcta cctgatcaaa agtgacaagg
5401 gctattggtt gcccggtggc tttggttata cgtcaaacaa ggccgaggct ggccgctttt
5461 cagtcgctga tatggccagc cttaaccttg acggctgcac cttgtccttg ttccgcgaag
5521 acaagccttt cggccccggc aagtttctcg gtgactgata tgaaagacca aaaggacaag
5581 cagaccggcg acctgctggc cagccctgac gctgtacgcc aagcgcgata tgccgagcgc
5641 atgaaggcca aagggatgcg tcagcgcaag ttctggctga ccgacgacga atacgaggcg
5701 ctgcgcgagt gcctggaaga actcagagcg gcgcagggcg ggggtagtga ccccgccagc
5761 gcctaaccac caactgcctg caaaggaggc aatcaatggc tacccataag cctatcaata
5821 ttctggaggc gttcgcagca gcgccgccac cgctggacta cgttttgccc aacatggtgg
5881 ccggtacggt cggggcgctg gtgtcgcccg gtggtgccgg taaatccatg ctggccctgc
5941 aactggccgc acagattgca ggcgggccgg atctgctgga ggtgggcgaa ctgcccaccg
6001 gcccggtgat ctacctgccc gccgaagacc cgcccaccgc cattcatcac cgcctgcacg
6061 cccttggggc gcacctcagc gccgaggaac ggcaagccgt ggctgacggc ctgctgatcc
6121 agccgctgat cggcagcctg cccaacatca tggccccgga gtggttcgac ggcctcaagc
6181 gcgccgccga gggccgccgc ctgatggtgc tggacacgct gcgccggttc cacatcgagg
6241 aagaaaacgc cagcggcccc atggcccagg tcatcggtcg catggaggcc atcgccgccg
6301 ataccgggtg ctctatcgtg ttcctgcacc atgccagcaa gggcgcggcc atgatgggcg
6361 caggcgacca gcagcaggcc agccggggca gctcggtact ggtcgataac atccgctggc
6421 agtcctacct gtcgagcatg accagcgccg aggccgagga atggggtgtg gacgacgacc
6481 agcgccggtt cttcgtccgc ttcggtgtga gcaaggccaa ctatggcgca ccgttcgctg
6541 atcggtggtt caggcggcat gacggcgggg tgctcaagcc cgccgtgctg gagaggcagc
6601 gcaagagcaa gggggtgccc cgtggtgaag cctaagaaca agcacagcct cagccacgtc
6661 cggcacgacc cggcgcactg tctggccccc ggcctgttcc gtgccctcaa gcggggcgag
6721 cgcaagcgca gcaagctgga cgtgacgtat gactacggcg acggcaagcg gatcgagttc
6781 agcggcccgg agccgctggg cgctgatgat ctgcgcatcc tgcaagggct ggtggccatg
6841 gctgggccta atggcctagt gcttggcccg gaacccaaga ccgaaggcgg acggcagctc
6901 cggctgttcc tggaacccaa gtgggaggcc gtcaccgctg aatgccatgt ggtcaaaggt
6961 agctatcggg cgctggcaaa ggaaatcggg gcagaggtcg atagtggtgg ggcgctcaag
7021 cacatacagg actgcatcga gcgcctttgg aaggtatcca tcatcgccca gaatggccgc
7081 aagcggcagg ggtttcggct gctgtcggag tacgccagcg acgaggcgga cgggcgcctg
7141 tacgtggccc tgaacccctt gatcgcgcag gccgtcatgg gtggcggcca gcatgtgcgc
7201 atcagcatgg acgaggtgcg ggcgctggac agcgaaaccg cccgcctgct gcaccagcgg
7261 ctgtgtggct ggatcgaccc cggcaaaacc ggcaaggctt ccatagatac cttgtgcggc
7321 tatgtctggc cgtcagaggc cagtggttcg accatgcgca agcgccgcaa gcgggtgcgc
7381 gaggcgttgc cggagctggt cgcgctgggc tggacggtaa ccgagttcgc ggcgggcaag
7441 tacgacatca cccggcccaa ggcggcaggc tgaccccccc cactctattg taaacaagac
7501 atttttatct tttatattca atggcttatt ttcctgctaa ttggtaatac catgaaaaat
7561 accatgctca gaaaaggctt aacaatattt tgaaaaattg cctactgagc gctgccgcac
7621 agctccatag gccgctttcc tggctttgct tccagatgta tgctcttctg ctcccgaacg
7681 ccagcaagac gtagcccagc gcgtcggcca gcttgcaatt cgcgctaact tacattaatt
7741 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
7801 atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt
7861 caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag
7921 caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg
7981 cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc
8041 aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc
8101 aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc
8161 ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag
8221 atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa
8281 cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc
8341 ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc
8401 cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt
8461 aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc
8521 gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga
8581 tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc
8641 aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag
8701 ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt
8761 caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt
8821 tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc
8881 gcgaaaggtt ttgcaccatt cgatggtgtc aacgtaaatg ccgcttcgcc ttcgcgcgcg
8941 aattgcaagc tgatccgggc ttatcgactg cacggtgcac caatgcttct ggcgtcaggc
9001 agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc
9061 tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca
9121 aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg
9181 agcggataac aatttcacac aggaaactag gcaccccagg ctttacactt tatgcttccg
9241 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
9301 catgattacg aa
//GenBank-Updates@genbank.bio.net (03/02/91)
LOCUS PMMB206 9312 bp Circular SYN 02-MAR-1991
DEFINITION Bacterial cloning vector pMMB206, complete genome.
ACCESSION M37847
KEYWORDS alpha peptide; beta-lactamase; chloramphenicol acetyl transferase;
conjugational DNA transfer protein; helicase; lac repressor;
primase; regulatory protein; replication initiation protein.
SOURCE Cloning vector pMMB206 DNA.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (bases 1 to 9312)
AUTHORS Morales,V.M., Backman,A. and Bagdasarian,M.
TITLE A series of broad-host-range low-copy-number cloning vectors that
allow direct screening for recombinants
JOURNAL Unpublished (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by V.M.Morales, 21-AUG-1990.
Natl Res Council, Canada
Plant Biotech Inst
Saskatoon, Sask.
S7N OW9 Canada
FEATURES Location/Qualifiers
CDS join(9295..9311,1..181)
/product="alfa peptide"
/gene="lacZ'"
/codon_start=9295
promoter 638..668
/gene="bla"
CDS 708..941
/note="truncated"
/product="beta-lactamase"
/gene="bla"
/codon_start=708
promoter 1168..1194
/gene="cat"
CDS 1261..1920
/product="chloramphenicol acetyl transferase"
/gene="cat"
/codon_start=1261
rep_origin 2234..2647
CDS complement(2673..2957)
/product="conjugational DNA transfer protein"
/gene="mobC"
/codon_start=2955
misc_feature 2910..3067
/note="origin of conjugational DNA transfer"
promoter 2968..2998
/note="P3"
CDS 3156..5285
/product="primase"
/gene="repB/mobA"
/codon_start=3156
CDS 3904..4317
/product="conjugational DNA transfer protein"
/gene="mobB"
/codon_start=3904
CDS 4314..5285
/product="primase"
/gene="repB'"
/codon_start=4314
promoter 5287..5318
/note="P4"
CDS 5346..5558
/note="protein of unknown function"
/gene="E"
/codon_start=5346
CDS 5560..5766
/note="Regulates repC and repA gene expression."
/product="regulatory protein"
/gene="cac"
/codon_start=5560
CDS 5796..6635
/product="DNA helicase"
/gene="repA"
/codon_start=5796
CDS 6622..7473
/product="replication initiator protein"
/gene="repC"
/codon_start=6622
promoter 7634..7664
/note="P5"
CDS complement(7750..8832)
/note="stop codons in coding region."
/product="lac repressor"
/gene="lacI"
/codon_start=8830
promoter 9140..9168
/gene="tac"
promoter 9223..9251
/gene="lacUV5"
BASE COUNT 2042 a 2693 c 2726 g 1851 t
ORIGIN
1 ttcccgggga tccgtcgacc tgcagccaag cttggcactg gccgtcgttt tacaacgtcg
61 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
121 cagctggcta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
181 aatggcgatg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
241 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
301 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
361 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
421 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
481 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
541 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
601 gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
661 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
721 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
781 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
841 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgtgatg
901 tccggcggtg cttttgccgt tacgcaccac cccgtcagta gctgaacagg agggacagct
961 gatagaaaca gaagccactg gagcaagcac ctcaaaaaca ccatcataca ctaaatcagt
1021 aagttggcag catcacccga cgcactttgc gccgaataaa tacctgtgac ggaagatcac
1081 ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt
1141 tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata
1201 agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa
1261 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
1321 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
1381 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
1441 cacattcttg cccgcctgat gaatgctcat ccggaattac gtatggcaat gaaagacggt
1501 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
1561 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
1621 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
1681 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
1741 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
1801 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
1861 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
1921 tttttttaag gcagttattg tcttcaaatt cccgttgcac atagcccggc aattcctttc
1981 cctgctctgc cataagcgca gcgaatgccg ggtaatactc gtcaacgatc tgatagagaa
2041 gggtttgctc gggtcggtgg ctctggtaac gaccagtatc ccgatcccgg ctggccgtcc
2101 tggccgccac atgaggcatg ttccgcgtcc ttgcaatact gtgtttacat acagtctatc
2161 gcttagcgga aagttctttt accctcagcc gaaatgcctg ccgttgctag acattgccag
2221 ccagtgcccg tcactcccgt actaactgtc acgaacccct gcaataactg tcacgccccc
2281 ctgcaataac tgtcacgaac ccctgcaata actgtcacgc ccccaaacct gcaaacccag
2341 caggggcggg ggctggcggg gtgttggaaa aatccatcca tgattatcta agaataatcc
2401 actaggcgcg gttatcagcg cccttgtggg gcgctgctgc ccttgcccaa tatgcccggc
2461 cagaggccgg atagctggtc tattcgctgc gctaggctac acaccgcccc accgctgcgc
2521 ggcaggggga aaggcgggca aagcccgcta aaccccacac caaaccccgc agaaatacgc
2581 tggagcgctt ttagccgctt tagcggcctt tccccctacc cgaagggtgg gggcgcgtgt
2641 gcagccccgc agggcctgtc tcggtcgatc attcagcccg gctcatcctt ctggcgtggc
2701 ggcagaccga acaaggcgcg gtcgtggtcg cgttcaaggt acgcatccat tgccgccatg
2761 agccgatcct ccggccactc gctgctgttc accttggcca aaatcatggc ccccaccagc
2821 accttgcgcc ttgtttcgtt cttgcgctct tgctgctgtt cccttgcccg ctcccgctga
2881 atttcggcat tgattcgcgc tcgttgttct tcgagcttgg ccagccgatc cgccgccttg
2941 ttgctcccct taaccatctt gacaccccat tgttaatgtg ctgtctcgta ggctatcatg
3001 gaggcacagc ggcggcaatc ccgaccctac tttgtagggg agggcgcact taccggtttc
3061 tcttcgagaa actggcctaa cggccaccct tcgggcggtg cgctctccga gggccattgc
3121 atggagccga aaagcaaaag caacagcgag gcagcatggc gatttatcac cttacggcga
3181 aaaccggcag caggtcgggc ggccaatcgg ccagggccaa ggccgactac atccagcgcg
3241 aaggcaagta tgcccgcgac atggatgaag tcttgcacgc cgaatccggg cacatgccgg
3301 agttcgtcga gcggcccgcc gactactggg atgctgccga cctgtatgaa cgcgccaatg
3361 ggcggctgtt caaggaggtc gaatttgccc tgccggtcga gctgaccctc gaccagcaga
3421 aggcgctggc gtccgagttc gcccagcacc tgaccggtgc cgagcgcctg ccgtatacgc
3481 tggccatcca tgccggtggc ggcgagaacc cgcactgcca cctgatgatc tccgagcgga
3541 tcaatgacgg catcgagcgg cccgccgctc agtggttcaa gcggtacaac ggcaagaccc
3601 cggagaaggg cggggcacag aagaccgaag cgctcaagcc caaggcatgg cttgagcaga
3661 cccgcgaggc atgggccgac catgccaacc gggcattaga gcgggctggc cacgacgccc
3721 gcattgacca cagaacactt gaggcgcagg gcatcgagcg cctgcccggt gttcacctgg
3781 ggccgaacgt ggtggagatg gaaggccggg gcatccgcac cgaccgggca gacgtggccc
3841 tgaacatcga caccgccaac gcccagatca tcgacttaca ggaataccgg gaggcaatag
3901 accatgaacg caatcgacag agtgaagaaa tccagaggca tcaacgagtt agcggagcag
3961 atcgaaccgc tggcccagag catggcgaca ctggccgacg aagcccggca ggtcatgagc
4021 cagaccaagc aggccagcga ggcgcaggcg gcggagtggc tgaaagccca gcgccagaca
4081 ggggcggcat gggtggagct ggccaaagag ttgcgggagg tagccgccga ggtgagcagc
4141 gccgcgcaga gcgcccggag cgcgtcgcgg gggtggcact ggaagctatg gctaaccgtg
4201 atgctggctt ccatgatgcc tacggtggtg ctgctgatcg catcgttgct cttgctcgac
4261 ctgacgccac tgacaaccga ggacggctcg atctggctgc gcttggtggc ccgatgaaga
4321 acgacaggac tttgcaggcc ataggccgac agctcaaggc catgggctgt gagcgcttcg
4381 atatcggcgt cagggacgca cccaccggcc agatgatgaa ccgggaatgg tcagccgccg
4441 aagtgctcca gaacacgcca tggctcaagc ggatgaatgc ccagggcaat gacgtgtata
4501 tcaggcccgc cgagcaggag cggcatggtc tggtgctggt ggacgacctc agcgagtttg
4561 acctggatga catgaaagcc gagggccggg agcctgccct ggtagtggaa accagcccga
4621 agaactatca ggcatgggtc aaggtggccg acgccgcagg cggtgaactt cgggggcaga
4681 ttgcccggac gctggccagc gagtacgacg ccgacccggc cagcgccgac agccgccact
4741 atggccgctt ggcgggcttc accaaccgca aggacaagca caccacccgc gccggttatc
4801 agccgtgggt gctgctgcgt gaatccaagg gcaagaccgc caccgctggc ccggcgctgg
4861 tgcagcaggc tggccagcag atcgagcagg cccagcggca gcaggagaag gcccgcaggc
4921 tggccagcct cgaactgccc gagcggcagc ttagccgcca ccggcgcacg gcgctggacg
4981 agtaccgcag cgagatggcc gggctggtca agcgcttcgg tcatgacctc agcaagtgcg
5041 actttatcgc cgcgcagaag ctggccagcc ggggccgcag tgccgaggaa atcggcaagg
5101 ccatggccga ggccagccca gcgctggcag agcgcaagcc cggccacgaa gcggattaca
5161 tcgagcgcac cgtcagcaag gtcatgggtc tgcccagcgt ccagcttgcg cgggccgagc
5221 tggcacgggc accggcaccc cgccagcgag gcatggacag gggcgggcca gatttcagca
5281 tgtagtgctt gcgttggtac tcacgcctgt tatactatga gtactcacgc acagaagggg
5341 gttttatgga atacgaaaaa agcgcttcag ggtcggtcta cctgatcaaa agtgacaagg
5401 gctattggtt gcccggtggc tttggttata cgtcaaacaa ggccgaggct ggccgctttt
5461 cagtcgctga tatggccagc cttaaccttg acggctgcac cttgtccttg ttccgcgaag
5521 acaagccttt cggccccggc aagtttctcg gtgactgata tgaaagacca aaaggacaag
5581 cagaccggcg acctgctggc cagccctgac gctgtacgcc aagcgcgata tgccgagcgc
5641 atgaaggcca aagggatgcg tcagcgcaag ttctggctga ccgacgacga atacgaggcg
5701 ctgcgcgagt gcctggaaga actcagagcg gcgcagggcg ggggtagtga ccccgccagc
5761 gcctaaccac caactgcctg caaaggaggc aatcaatggc tacccataag cctatcaata
5821 ttctggaggc gttcgcagca gcgccgccac cgctggacta cgttttgccc aacatggtgg
5881 ccggtacggt cggggcgctg gtgtcgcccg gtggtgccgg taaatccatg ctggccctgc
5941 aactggccgc acagattgca ggcgggccgg atctgctgga ggtgggcgaa ctgcccaccg
6001 gcccggtgat ctacctgccc gccgaagacc cgcccaccgc cattcatcac cgcctgcacg
6061 cccttggggc gcacctcagc gccgaggaac ggcaagccgt ggctgacggc ctgctgatcc
6121 agccgctgat cggcagcctg cccaacatca tggccccgga gtggttcgac ggcctcaagc
6181 gcgccgccga gggccgccgc ctgatggtgc tggacacgct gcgccggttc cacatcgagg
6241 aagaaaacgc cagcggcccc atggcccagg tcatcggtcg catggaggcc atcgccgccg
6301 ataccgggtg ctctatcgtg ttcctgcacc atgccagcaa gggcgcggcc atgatgggcg
6361 caggcgacca gcagcaggcc agccggggca gctcggtact ggtcgataac atccgctggc
6421 agtcctacct gtcgagcatg accagcgccg aggccgagga atggggtgtg gacgacgacc
6481 agcgccggtt cttcgtccgc ttcggtgtga gcaaggccaa ctatggcgca ccgttcgctg
6541 atcggtggtt caggcggcat gacggcgggg tgctcaagcc cgccgtgctg gagaggcagc
6601 gcaagagcaa gggggtgccc cgtggtgaag cctaagaaca agcacagcct cagccacgtc
6661 cggcacgacc cggcgcactg tctggccccc ggcctgttcc gtgccctcaa gcggggcgag
6721 cgcaagcgca gcaagctgga cgtgacgtat gactacggcg acggcaagcg gatcgagttc
6781 agcggcccgg agccgctggg cgctgatgat ctgcgcatcc tgcaagggct ggtggccatg
6841 gctgggccta atggcctagt gcttggcccg gaacccaaga ccgaaggcgg acggcagctc
6901 cggctgttcc tggaacccaa gtgggaggcc gtcaccgctg aatgccatgt ggtcaaaggt
6961 agctatcggg cgctggcaaa ggaaatcggg gcagaggtcg atagtggtgg ggcgctcaag
7021 cacatacagg actgcatcga gcgcctttgg aaggtatcca tcatcgccca gaatggccgc
7081 aagcggcagg ggtttcggct gctgtcggag tacgccagcg acgaggcgga cgggcgcctg
7141 tacgtggccc tgaacccctt gatcgcgcag gccgtcatgg gtggcggcca gcatgtgcgc
7201 atcagcatgg acgaggtgcg ggcgctggac agcgaaaccg cccgcctgct gcaccagcgg
7261 ctgtgtggct ggatcgaccc cggcaaaacc ggcaaggctt ccatagatac cttgtgcggc
7321 tatgtctggc cgtcagaggc cagtggttcg accatgcgca agcgccgcaa gcgggtgcgc
7381 gaggcgttgc cggagctggt cgcgctgggc tggacggtaa ccgagttcgc ggcgggcaag
7441 tacgacatca cccggcccaa ggcggcaggc tgaccccccc cactctattg taaacaagac
7501 atttttatct tttatattca atggcttatt ttcctgctaa ttggtaatac catgaaaaat
7561 accatgctca gaaaaggctt aacaatattt tgaaaaattg cctactgagc gctgccgcac
7621 agctccatag gccgctttcc tggctttgct tccagatgta tgctcttctg ctcccgaacg
7681 ccagcaagac gtagcccagc gcgtcggcca gcttgcaatt cgcgctaact tacattaatt
7741 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
7801 atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt
7861 caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag
7921 caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg
7981 cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc
8041 aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc
8101 aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc
8161 ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag
8221 atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa
8281 cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc
8341 ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc
8401 cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt
8461 aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc
8521 gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga
8581 tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc
8641 aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag
8701 ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt
8761 caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt
8821 tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc
8881 gcgaaaggtt ttgcaccatt cgatggtgtc aacgtaaatg ccgcttcgcc ttcgcgcgcg
8941 aattgcaagc tgatccgggc ttatcgactg cacggtgcac caatgcttct ggcgtcaggc
9001 agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc
9061 tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca
9121 aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg
9181 agcggataac aatttcacac aggaaactag gcaccccagg ctttacactt tatgcttccg
9241 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
9301 catgattacg aa
//GenBank-Updates@genbank.bio.net (03/07/91)
LOCUS PMMB206 9312 bp Circular SYN 07-MAR-1991
DEFINITION Bacterial cloning vector pMMB206, complete genome.
ACCESSION M37847
KEYWORDS alpha peptide; beta-lactamase; chloramphenicol acetyl transferase;
conjugational DNA transfer protein; helicase; lac repressor;
primase; regulatory protein; replication initiation protein.
SOURCE Cloning vector pMMB206 DNA.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (bases 1 to 9312)
AUTHORS Morales,V.M., Backman,A. and Bagdasarian,M.
TITLE A series of broad-host-range low-copy-number cloning vectors that
allow direct screening for recombinants
JOURNAL Unpublished (1990)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by V.M.Morales, 21-AUG-1990.
Natl Res Council, Canada
Plant Biotech Inst
Saskatoon, Sask.
S7N OW9 Canada
FEATURES Location/Qualifiers
CDS join(9295..9311,1..181)
/product="alpha peptide"
/gene="lacZ'"
/codon_start=9295
promoter 638..668
/gene="bla"
CDS 708..941
/note="truncated"
/product="beta-lactamase"
/gene="bla"
/codon_start=708
promoter 1168..1194
/gene="cat"
CDS 1261..1920
/product="chloramphenicol acetyl transferase"
/gene="cat"
/codon_start=1261
rep_origin 2234..2647
CDS complement(2673..2957)
/product="conjugational DNA transfer protein"
/gene="mobC"
/codon_start=2955
misc_feature 2910..3067
/note="origin of conjugational DNA transfer"
promoter 2968..2998
/note="P3"
CDS 3156..5285
/product="primase"
/gene="repB/mobA"
/codon_start=3156
CDS 3904..4317
/product="conjugational DNA transfer protein"
/gene="mobB"
/codon_start=3904
CDS 4314..5285
/product="primase"
/gene="repB'"
/codon_start=4314
promoter 5287..5318
/note="P4"
CDS 5346..5558
/note="protein of unknown function"
/gene="E"
/codon_start=5346
CDS 5560..5766
/note="Regulates repC and repA gene expression."
/product="regulatory protein"
/gene="cac"
/codon_start=5560
CDS 5796..6635
/product="DNA helicase"
/gene="repA"
/codon_start=5796
CDS 6622..7473
/product="replication initiator protein"
/gene="repC"
/codon_start=6622
promoter 7634..7664
/note="P5"
CDS complement(7750..8832)
/note="stop codons in coding region."
/product="lac repressor"
/gene="lacI"
/codon_start=8830
promoter 9140..9168
/gene="tac"
promoter 9223..9251
/gene="lacUV5"
BASE COUNT 2042 a 2693 c 2726 g 1851 t
ORIGIN
1 ttcccgggga tccgtcgacc tgcagccaag cttggcactg gccgtcgttt tacaacgtcg
61 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
121 cagctggcta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
181 aatggcgatg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
241 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
301 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
361 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
421 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
481 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
541 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
601 gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
661 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
721 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
781 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
841 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgtgatg
901 tccggcggtg cttttgccgt tacgcaccac cccgtcagta gctgaacagg agggacagct
961 gatagaaaca gaagccactg gagcaagcac ctcaaaaaca ccatcataca ctaaatcagt
1021 aagttggcag catcacccga cgcactttgc gccgaataaa tacctgtgac ggaagatcac
1081 ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt
1141 tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata
1201 agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa
1261 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
1321 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
1381 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
1441 cacattcttg cccgcctgat gaatgctcat ccggaattac gtatggcaat gaaagacggt
1501 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
1561 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
1621 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
1681 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
1741 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
1801 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
1861 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
1921 tttttttaag gcagttattg tcttcaaatt cccgttgcac atagcccggc aattcctttc
1981 cctgctctgc cataagcgca gcgaatgccg ggtaatactc gtcaacgatc tgatagagaa
2041 gggtttgctc gggtcggtgg ctctggtaac gaccagtatc ccgatcccgg ctggccgtcc
2101 tggccgccac atgaggcatg ttccgcgtcc ttgcaatact gtgtttacat acagtctatc
2161 gcttagcgga aagttctttt accctcagcc gaaatgcctg ccgttgctag acattgccag
2221 ccagtgcccg tcactcccgt actaactgtc acgaacccct gcaataactg tcacgccccc
2281 ctgcaataac tgtcacgaac ccctgcaata actgtcacgc ccccaaacct gcaaacccag
2341 caggggcggg ggctggcggg gtgttggaaa aatccatcca tgattatcta agaataatcc
2401 actaggcgcg gttatcagcg cccttgtggg gcgctgctgc ccttgcccaa tatgcccggc
2461 cagaggccgg atagctggtc tattcgctgc gctaggctac acaccgcccc accgctgcgc
2521 ggcaggggga aaggcgggca aagcccgcta aaccccacac caaaccccgc agaaatacgc
2581 tggagcgctt ttagccgctt tagcggcctt tccccctacc cgaagggtgg gggcgcgtgt
2641 gcagccccgc agggcctgtc tcggtcgatc attcagcccg gctcatcctt ctggcgtggc
2701 ggcagaccga acaaggcgcg gtcgtggtcg cgttcaaggt acgcatccat tgccgccatg
2761 agccgatcct ccggccactc gctgctgttc accttggcca aaatcatggc ccccaccagc
2821 accttgcgcc ttgtttcgtt cttgcgctct tgctgctgtt cccttgcccg ctcccgctga
2881 atttcggcat tgattcgcgc tcgttgttct tcgagcttgg ccagccgatc cgccgccttg
2941 ttgctcccct taaccatctt gacaccccat tgttaatgtg ctgtctcgta ggctatcatg
3001 gaggcacagc ggcggcaatc ccgaccctac tttgtagggg agggcgcact taccggtttc
3061 tcttcgagaa actggcctaa cggccaccct tcgggcggtg cgctctccga gggccattgc
3121 atggagccga aaagcaaaag caacagcgag gcagcatggc gatttatcac cttacggcga
3181 aaaccggcag caggtcgggc ggccaatcgg ccagggccaa ggccgactac atccagcgcg
3241 aaggcaagta tgcccgcgac atggatgaag tcttgcacgc cgaatccggg cacatgccgg
3301 agttcgtcga gcggcccgcc gactactggg atgctgccga cctgtatgaa cgcgccaatg
3361 ggcggctgtt caaggaggtc gaatttgccc tgccggtcga gctgaccctc gaccagcaga
3421 aggcgctggc gtccgagttc gcccagcacc tgaccggtgc cgagcgcctg ccgtatacgc
3481 tggccatcca tgccggtggc ggcgagaacc cgcactgcca cctgatgatc tccgagcgga
3541 tcaatgacgg catcgagcgg cccgccgctc agtggttcaa gcggtacaac ggcaagaccc
3601 cggagaaggg cggggcacag aagaccgaag cgctcaagcc caaggcatgg cttgagcaga
3661 cccgcgaggc atgggccgac catgccaacc gggcattaga gcgggctggc cacgacgccc
3721 gcattgacca cagaacactt gaggcgcagg gcatcgagcg cctgcccggt gttcacctgg
3781 ggccgaacgt ggtggagatg gaaggccggg gcatccgcac cgaccgggca gacgtggccc
3841 tgaacatcga caccgccaac gcccagatca tcgacttaca ggaataccgg gaggcaatag
3901 accatgaacg caatcgacag agtgaagaaa tccagaggca tcaacgagtt agcggagcag
3961 atcgaaccgc tggcccagag catggcgaca ctggccgacg aagcccggca ggtcatgagc
4021 cagaccaagc aggccagcga ggcgcaggcg gcggagtggc tgaaagccca gcgccagaca
4081 ggggcggcat gggtggagct ggccaaagag ttgcgggagg tagccgccga ggtgagcagc
4141 gccgcgcaga gcgcccggag cgcgtcgcgg gggtggcact ggaagctatg gctaaccgtg
4201 atgctggctt ccatgatgcc tacggtggtg ctgctgatcg catcgttgct cttgctcgac
4261 ctgacgccac tgacaaccga ggacggctcg atctggctgc gcttggtggc ccgatgaaga
4321 acgacaggac tttgcaggcc ataggccgac agctcaaggc catgggctgt gagcgcttcg
4381 atatcggcgt cagggacgca cccaccggcc agatgatgaa ccgggaatgg tcagccgccg
4441 aagtgctcca gaacacgcca tggctcaagc ggatgaatgc ccagggcaat gacgtgtata
4501 tcaggcccgc cgagcaggag cggcatggtc tggtgctggt ggacgacctc agcgagtttg
4561 acctggatga catgaaagcc gagggccggg agcctgccct ggtagtggaa accagcccga
4621 agaactatca ggcatgggtc aaggtggccg acgccgcagg cggtgaactt cgggggcaga
4681 ttgcccggac gctggccagc gagtacgacg ccgacccggc cagcgccgac agccgccact
4741 atggccgctt ggcgggcttc accaaccgca aggacaagca caccacccgc gccggttatc
4801 agccgtgggt gctgctgcgt gaatccaagg gcaagaccgc caccgctggc ccggcgctgg
4861 tgcagcaggc tggccagcag atcgagcagg cccagcggca gcaggagaag gcccgcaggc
4921 tggccagcct cgaactgccc gagcggcagc ttagccgcca ccggcgcacg gcgctggacg
4981 agtaccgcag cgagatggcc gggctggtca agcgcttcgg tcatgacctc agcaagtgcg
5041 actttatcgc cgcgcagaag ctggccagcc ggggccgcag tgccgaggaa atcggcaagg
5101 ccatggccga ggccagccca gcgctggcag agcgcaagcc cggccacgaa gcggattaca
5161 tcgagcgcac cgtcagcaag gtcatgggtc tgcccagcgt ccagcttgcg cgggccgagc
5221 tggcacgggc accggcaccc cgccagcgag gcatggacag gggcgggcca gatttcagca
5281 tgtagtgctt gcgttggtac tcacgcctgt tatactatga gtactcacgc acagaagggg
5341 gttttatgga atacgaaaaa agcgcttcag ggtcggtcta cctgatcaaa agtgacaagg
5401 gctattggtt gcccggtggc tttggttata cgtcaaacaa ggccgaggct ggccgctttt
5461 cagtcgctga tatggccagc cttaaccttg acggctgcac cttgtccttg ttccgcgaag
5521 acaagccttt cggccccggc aagtttctcg gtgactgata tgaaagacca aaaggacaag
5581 cagaccggcg acctgctggc cagccctgac gctgtacgcc aagcgcgata tgccgagcgc
5641 atgaaggcca aagggatgcg tcagcgcaag ttctggctga ccgacgacga atacgaggcg
5701 ctgcgcgagt gcctggaaga actcagagcg gcgcagggcg ggggtagtga ccccgccagc
5761 gcctaaccac caactgcctg caaaggaggc aatcaatggc tacccataag cctatcaata
5821 ttctggaggc gttcgcagca gcgccgccac cgctggacta cgttttgccc aacatggtgg
5881 ccggtacggt cggggcgctg gtgtcgcccg gtggtgccgg taaatccatg ctggccctgc
5941 aactggccgc acagattgca ggcgggccgg atctgctgga ggtgggcgaa ctgcccaccg
6001 gcccggtgat ctacctgccc gccgaagacc cgcccaccgc cattcatcac cgcctgcacg
6061 cccttggggc gcacctcagc gccgaggaac ggcaagccgt ggctgacggc ctgctgatcc
6121 agccgctgat cggcagcctg cccaacatca tggccccgga gtggttcgac ggcctcaagc
6181 gcgccgccga gggccgccgc ctgatggtgc tggacacgct gcgccggttc cacatcgagg
6241 aagaaaacgc cagcggcccc atggcccagg tcatcggtcg catggaggcc atcgccgccg
6301 ataccgggtg ctctatcgtg ttcctgcacc atgccagcaa gggcgcggcc atgatgggcg
6361 caggcgacca gcagcaggcc agccggggca gctcggtact ggtcgataac atccgctggc
6421 agtcctacct gtcgagcatg accagcgccg aggccgagga atggggtgtg gacgacgacc
6481 agcgccggtt cttcgtccgc ttcggtgtga gcaaggccaa ctatggcgca ccgttcgctg
6541 atcggtggtt caggcggcat gacggcgggg tgctcaagcc cgccgtgctg gagaggcagc
6601 gcaagagcaa gggggtgccc cgtggtgaag cctaagaaca agcacagcct cagccacgtc
6661 cggcacgacc cggcgcactg tctggccccc ggcctgttcc gtgccctcaa gcggggcgag
6721 cgcaagcgca gcaagctgga cgtgacgtat gactacggcg acggcaagcg gatcgagttc
6781 agcggcccgg agccgctggg cgctgatgat ctgcgcatcc tgcaagggct ggtggccatg
6841 gctgggccta atggcctagt gcttggcccg gaacccaaga ccgaaggcgg acggcagctc
6901 cggctgttcc tggaacccaa gtgggaggcc gtcaccgctg aatgccatgt ggtcaaaggt
6961 agctatcggg cgctggcaaa ggaaatcggg gcagaggtcg atagtggtgg ggcgctcaag
7021 cacatacagg actgcatcga gcgcctttgg aaggtatcca tcatcgccca gaatggccgc
7081 aagcggcagg ggtttcggct gctgtcggag tacgccagcg acgaggcgga cgggcgcctg
7141 tacgtggccc tgaacccctt gatcgcgcag gccgtcatgg gtggcggcca gcatgtgcgc
7201 atcagcatgg acgaggtgcg ggcgctggac agcgaaaccg cccgcctgct gcaccagcgg
7261 ctgtgtggct ggatcgaccc cggcaaaacc ggcaaggctt ccatagatac cttgtgcggc
7321 tatgtctggc cgtcagaggc cagtggttcg accatgcgca agcgccgcaa gcgggtgcgc
7381 gaggcgttgc cggagctggt cgcgctgggc tggacggtaa ccgagttcgc ggcgggcaag
7441 tacgacatca cccggcccaa ggcggcaggc tgaccccccc cactctattg taaacaagac
7501 atttttatct tttatattca atggcttatt ttcctgctaa ttggtaatac catgaaaaat
7561 accatgctca gaaaaggctt aacaatattt tgaaaaattg cctactgagc gctgccgcac
7621 agctccatag gccgctttcc tggctttgct tccagatgta tgctcttctg ctcccgaacg
7681 ccagcaagac gtagcccagc gcgtcggcca gcttgcaatt cgcgctaact tacattaatt
7741 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
7801 atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt
7861 caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag
7921 caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg
7981 cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc
8041 aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc
8101 aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc
8161 ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag
8221 atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa
8281 cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc
8341 ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc
8401 cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt
8461 aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc
8521 gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga
8581 tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc
8641 aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag
8701 ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt
8761 caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt
8821 tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc
8881 gcgaaaggtt ttgcaccatt cgatggtgtc aacgtaaatg ccgcttcgcc ttcgcgcgcg
8941 aattgcaagc tgatccgggc ttatcgactg cacggtgcac caatgcttct ggcgtcaggc
9001 agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc
9061 tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca
9121 aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg
9181 agcggataac aatttcacac aggaaactag gcaccccagg ctttacactt tatgcttccg
9241 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
9301 catgattacg aa
//GenBank-Updates@genbank.bio.net (03/20/91)
LOCUS PMMB206 9312 bp Circular SYN 20-MAR-1991
DEFINITION Bacterial cloning vector pMMB206, complete genome.
ACCESSION M37847
KEYWORDS alpha peptide; beta-lactamase; chloramphenicol acetyl transferase;
conjugational DNA transfer protein; helicase; lac repressor;
primase; regulatory protein; replication initiation protein.
SOURCE Cloning vector pMMB206 DNA.
ORGANISM Cloning vector
Artificial sequences; Cloning vehicles.
REFERENCE 1 (bases 1 to 9312)
AUTHORS Morales,V.M., Baeckman,A. and Bagdasarian,M.
TITLE A series of wide-host-range low-copy-number vectors that allow
direct screening for recombinants
JOURNAL Gene 97, 39-47 (1991)
STANDARD simple staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by V.M.Morales, 21-AUG-1990.
Natl Res Council, Canada
Plant Biotech Inst
Saskatoon, Sask.
S7N OW9 Canada
FEATURES Location/Qualifiers
CDS join(9295..9311,1..181)
/product="alpha peptide"
/gene="lacZ'"
/codon_start=9295
promoter 638..668
/gene="bla"
CDS 708..941
/note="truncated"
/product="beta-lactamase"
/gene="bla"
/codon_start=708
promoter 1168..1194
/gene="cat"
CDS 1261..1920
/product="chloramphenicol acetyl transferase"
/gene="cat"
/codon_start=1261
rep_origin 2234..2647
CDS complement(2673..2957)
/product="conjugational DNA transfer protein"
/gene="mobC"
/codon_start=2955
misc_feature 2910..3067
/note="origin of conjugational DNA transfer"
promoter 2968..2998
/note="P3"
CDS 3156..5285
/product="primase"
/gene="repB/mobA"
/codon_start=3156
CDS 3904..4317
/product="conjugational DNA transfer protein"
/gene="mobB"
/codon_start=3904
CDS 4314..5285
/product="primase"
/gene="repB'"
/codon_start=4314
promoter 5287..5318
/note="P4"
CDS 5346..5558
/note="protein of unknown function"
/gene="E"
/codon_start=5346
CDS 5560..5766
/note="Regulates repC and repA gene expression."
/product="regulatory protein"
/gene="cac"
/codon_start=5560
CDS 5796..6635
/product="DNA helicase"
/gene="repA"
/codon_start=5796
CDS 6622..7473
/product="replication initiator protein"
/gene="repC"
/codon_start=6622
promoter 7634..7664
/note="P5"
CDS complement(7750..8832)
/note="stop codons in coding region."
/product="lac repressor"
/gene="lacI"
/codon_start=8830
promoter 9140..9168
/gene="tac"
promoter 9223..9251
/gene="lacUV5"
BASE COUNT 2042 a 2693 c 2726 g 1851 t
ORIGIN
1 ttcccgggga tccgtcgacc tgcagccaag cttggcactg gccgtcgttt tacaacgtcg
61 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
121 cagctggcta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
181 aatggcgatg gctgttttgg cggatgagag aagattttca gcctgataca gattaaatca
241 gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca
301 cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct
361 ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga
421 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
481 gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg caggacgccc
541 gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
601 gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
661 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
721 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
781 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
841 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgtgatg
901 tccggcggtg cttttgccgt tacgcaccac cccgtcagta gctgaacagg agggacagct
961 gatagaaaca gaagccactg gagcaagcac ctcaaaaaca ccatcataca ctaaatcagt
1021 aagttggcag catcacccga cgcactttgc gccgaataaa tacctgtgac ggaagatcac
1081 ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt
1141 tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca taatgaaata
1201 agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa ggaagctaaa
1261 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
1321 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
1381 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
1441 cacattcttg cccgcctgat gaatgctcat ccggaattac gtatggcaat gaaagacggt
1501 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
1561 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
1621 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
1681 aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
1741 gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
1801 gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
1861 gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
1921 tttttttaag gcagttattg tcttcaaatt cccgttgcac atagcccggc aattcctttc
1981 cctgctctgc cataagcgca gcgaatgccg ggtaatactc gtcaacgatc tgatagagaa
2041 gggtttgctc gggtcggtgg ctctggtaac gaccagtatc ccgatcccgg ctggccgtcc
2101 tggccgccac atgaggcatg ttccgcgtcc ttgcaatact gtgtttacat acagtctatc
2161 gcttagcgga aagttctttt accctcagcc gaaatgcctg ccgttgctag acattgccag
2221 ccagtgcccg tcactcccgt actaactgtc acgaacccct gcaataactg tcacgccccc
2281 ctgcaataac tgtcacgaac ccctgcaata actgtcacgc ccccaaacct gcaaacccag
2341 caggggcggg ggctggcggg gtgttggaaa aatccatcca tgattatcta agaataatcc
2401 actaggcgcg gttatcagcg cccttgtggg gcgctgctgc ccttgcccaa tatgcccggc
2461 cagaggccgg atagctggtc tattcgctgc gctaggctac acaccgcccc accgctgcgc
2521 ggcaggggga aaggcgggca aagcccgcta aaccccacac caaaccccgc agaaatacgc
2581 tggagcgctt ttagccgctt tagcggcctt tccccctacc cgaagggtgg gggcgcgtgt
2641 gcagccccgc agggcctgtc tcggtcgatc attcagcccg gctcatcctt ctggcgtggc
2701 ggcagaccga acaaggcgcg gtcgtggtcg cgttcaaggt acgcatccat tgccgccatg
2761 agccgatcct ccggccactc gctgctgttc accttggcca aaatcatggc ccccaccagc
2821 accttgcgcc ttgtttcgtt cttgcgctct tgctgctgtt cccttgcccg ctcccgctga
2881 atttcggcat tgattcgcgc tcgttgttct tcgagcttgg ccagccgatc cgccgccttg
2941 ttgctcccct taaccatctt gacaccccat tgttaatgtg ctgtctcgta ggctatcatg
3001 gaggcacagc ggcggcaatc ccgaccctac tttgtagggg agggcgcact taccggtttc
3061 tcttcgagaa actggcctaa cggccaccct tcgggcggtg cgctctccga gggccattgc
3121 atggagccga aaagcaaaag caacagcgag gcagcatggc gatttatcac cttacggcga
3181 aaaccggcag caggtcgggc ggccaatcgg ccagggccaa ggccgactac atccagcgcg
3241 aaggcaagta tgcccgcgac atggatgaag tcttgcacgc cgaatccggg cacatgccgg
3301 agttcgtcga gcggcccgcc gactactggg atgctgccga cctgtatgaa cgcgccaatg
3361 ggcggctgtt caaggaggtc gaatttgccc tgccggtcga gctgaccctc gaccagcaga
3421 aggcgctggc gtccgagttc gcccagcacc tgaccggtgc cgagcgcctg ccgtatacgc
3481 tggccatcca tgccggtggc ggcgagaacc cgcactgcca cctgatgatc tccgagcgga
3541 tcaatgacgg catcgagcgg cccgccgctc agtggttcaa gcggtacaac ggcaagaccc
3601 cggagaaggg cggggcacag aagaccgaag cgctcaagcc caaggcatgg cttgagcaga
3661 cccgcgaggc atgggccgac catgccaacc gggcattaga gcgggctggc cacgacgccc
3721 gcattgacca cagaacactt gaggcgcagg gcatcgagcg cctgcccggt gttcacctgg
3781 ggccgaacgt ggtggagatg gaaggccggg gcatccgcac cgaccgggca gacgtggccc
3841 tgaacatcga caccgccaac gcccagatca tcgacttaca ggaataccgg gaggcaatag
3901 accatgaacg caatcgacag agtgaagaaa tccagaggca tcaacgagtt agcggagcag
3961 atcgaaccgc tggcccagag catggcgaca ctggccgacg aagcccggca ggtcatgagc
4021 cagaccaagc aggccagcga ggcgcaggcg gcggagtggc tgaaagccca gcgccagaca
4081 ggggcggcat gggtggagct ggccaaagag ttgcgggagg tagccgccga ggtgagcagc
4141 gccgcgcaga gcgcccggag cgcgtcgcgg gggtggcact ggaagctatg gctaaccgtg
4201 atgctggctt ccatgatgcc tacggtggtg ctgctgatcg catcgttgct cttgctcgac
4261 ctgacgccac tgacaaccga ggacggctcg atctggctgc gcttggtggc ccgatgaaga
4321 acgacaggac tttgcaggcc ataggccgac agctcaaggc catgggctgt gagcgcttcg
4381 atatcggcgt cagggacgca cccaccggcc agatgatgaa ccgggaatgg tcagccgccg
4441 aagtgctcca gaacacgcca tggctcaagc ggatgaatgc ccagggcaat gacgtgtata
4501 tcaggcccgc cgagcaggag cggcatggtc tggtgctggt ggacgacctc agcgagtttg
4561 acctggatga catgaaagcc gagggccggg agcctgccct ggtagtggaa accagcccga
4621 agaactatca ggcatgggtc aaggtggccg acgccgcagg cggtgaactt cgggggcaga
4681 ttgcccggac gctggccagc gagtacgacg ccgacccggc cagcgccgac agccgccact
4741 atggccgctt ggcgggcttc accaaccgca aggacaagca caccacccgc gccggttatc
4801 agccgtgggt gctgctgcgt gaatccaagg gcaagaccgc caccgctggc ccggcgctgg
4861 tgcagcaggc tggccagcag atcgagcagg cccagcggca gcaggagaag gcccgcaggc
4921 tggccagcct cgaactgccc gagcggcagc ttagccgcca ccggcgcacg gcgctggacg
4981 agtaccgcag cgagatggcc gggctggtca agcgcttcgg tcatgacctc agcaagtgcg
5041 actttatcgc cgcgcagaag ctggccagcc ggggccgcag tgccgaggaa atcggcaagg
5101 ccatggccga ggccagccca gcgctggcag agcgcaagcc cggccacgaa gcggattaca
5161 tcgagcgcac cgtcagcaag gtcatgggtc tgcccagcgt ccagcttgcg cgggccgagc
5221 tggcacgggc accggcaccc cgccagcgag gcatggacag gggcgggcca gatttcagca
5281 tgtagtgctt gcgttggtac tcacgcctgt tatactatga gtactcacgc acagaagggg
5341 gttttatgga atacgaaaaa agcgcttcag ggtcggtcta cctgatcaaa agtgacaagg
5401 gctattggtt gcccggtggc tttggttata cgtcaaacaa ggccgaggct ggccgctttt
5461 cagtcgctga tatggccagc cttaaccttg acggctgcac cttgtccttg ttccgcgaag
5521 acaagccttt cggccccggc aagtttctcg gtgactgata tgaaagacca aaaggacaag
5581 cagaccggcg acctgctggc cagccctgac gctgtacgcc aagcgcgata tgccgagcgc
5641 atgaaggcca aagggatgcg tcagcgcaag ttctggctga ccgacgacga atacgaggcg
5701 ctgcgcgagt gcctggaaga actcagagcg gcgcagggcg ggggtagtga ccccgccagc
5761 gcctaaccac caactgcctg caaaggaggc aatcaatggc tacccataag cctatcaata
5821 ttctggaggc gttcgcagca gcgccgccac cgctggacta cgttttgccc aacatggtgg
5881 ccggtacggt cggggcgctg gtgtcgcccg gtggtgccgg taaatccatg ctggccctgc
5941 aactggccgc acagattgca ggcgggccgg atctgctgga ggtgggcgaa ctgcccaccg
6001 gcccggtgat ctacctgccc gccgaagacc cgcccaccgc cattcatcac cgcctgcacg
6061 cccttggggc gcacctcagc gccgaggaac ggcaagccgt ggctgacggc ctgctgatcc
6121 agccgctgat cggcagcctg cccaacatca tggccccgga gtggttcgac ggcctcaagc
6181 gcgccgccga gggccgccgc ctgatggtgc tggacacgct gcgccggttc cacatcgagg
6241 aagaaaacgc cagcggcccc atggcccagg tcatcggtcg catggaggcc atcgccgccg
6301 ataccgggtg ctctatcgtg ttcctgcacc atgccagcaa gggcgcggcc atgatgggcg
6361 caggcgacca gcagcaggcc agccggggca gctcggtact ggtcgataac atccgctggc
6421 agtcctacct gtcgagcatg accagcgccg aggccgagga atggggtgtg gacgacgacc
6481 agcgccggtt cttcgtccgc ttcggtgtga gcaaggccaa ctatggcgca ccgttcgctg
6541 atcggtggtt caggcggcat gacggcgggg tgctcaagcc cgccgtgctg gagaggcagc
6601 gcaagagcaa gggggtgccc cgtggtgaag cctaagaaca agcacagcct cagccacgtc
6661 cggcacgacc cggcgcactg tctggccccc ggcctgttcc gtgccctcaa gcggggcgag
6721 cgcaagcgca gcaagctgga cgtgacgtat gactacggcg acggcaagcg gatcgagttc
6781 agcggcccgg agccgctggg cgctgatgat ctgcgcatcc tgcaagggct ggtggccatg
6841 gctgggccta atggcctagt gcttggcccg gaacccaaga ccgaaggcgg acggcagctc
6901 cggctgttcc tggaacccaa gtgggaggcc gtcaccgctg aatgccatgt ggtcaaaggt
6961 agctatcggg cgctggcaaa ggaaatcggg gcagaggtcg atagtggtgg ggcgctcaag
7021 cacatacagg actgcatcga gcgcctttgg aaggtatcca tcatcgccca gaatggccgc
7081 aagcggcagg ggtttcggct gctgtcggag tacgccagcg acgaggcgga cgggcgcctg
7141 tacgtggccc tgaacccctt gatcgcgcag gccgtcatgg gtggcggcca gcatgtgcgc
7201 atcagcatgg acgaggtgcg ggcgctggac agcgaaaccg cccgcctgct gcaccagcgg
7261 ctgtgtggct ggatcgaccc cggcaaaacc ggcaaggctt ccatagatac cttgtgcggc
7321 tatgtctggc cgtcagaggc cagtggttcg accatgcgca agcgccgcaa gcgggtgcgc
7381 gaggcgttgc cggagctggt cgcgctgggc tggacggtaa ccgagttcgc ggcgggcaag
7441 tacgacatca cccggcccaa ggcggcaggc tgaccccccc cactctattg taaacaagac
7501 atttttatct tttatattca atggcttatt ttcctgctaa ttggtaatac catgaaaaat
7561 accatgctca gaaaaggctt aacaatattt tgaaaaattg cctactgagc gctgccgcac
7621 agctccatag gccgctttcc tggctttgct tccagatgta tgctcttctg ctcccgaacg
7681 ccagcaagac gtagcccagc gcgtcggcca gcttgcaatt cgcgctaact tacattaatt
7741 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
7801 atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt
7861 caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag
7921 caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg
7981 cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc
8041 aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc
8101 aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc
8161 ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag
8221 atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa
8281 cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc
8341 ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc
8401 cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt
8461 aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc
8521 gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga
8581 tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc
8641 aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag
8701 ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt
8761 caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt
8821 tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc
8881 gcgaaaggtt ttgcaccatt cgatggtgtc aacgtaaatg ccgcttcgcc ttcgcgcgcg
8941 aattgcaagc tgatccgggc ttatcgactg cacggtgcac caatgcttct ggcgtcaggc
9001 agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcataa ttcgtgtcgc
9061 tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac ggttctggca
9121 aatattctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg
9181 agcggataac aatttcacac aggaaactag gcaccccagg ctttacactt tatgcttccg
9241 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
9301 catgattacg aa
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