GenBank-Updates@genbank.bio.net (03/20/91)
LOCUS PFXBLUE 3627 bp ds-DNA SYN 20-MAR-1991
DEFINITION Synthetic E.coli pfxblue gene, complete cds.
ACCESSION M36415
KEYWORDS ampicillin resistance; cloning vector; fusion protein.
SOURCE Synthetic DNA.
ORGANISM Artificial gene
Artificial sequences; Genes.
REFERENCE 1 (sites)
AUTHORS Brandt,M.E., Gabrik,A.H. and Vickery,L.E.
TITLE A vector for directional cloning and expression of polymerase chain
reaction products in E.coli
JOURNAL Unpublished (1990)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 3627)
AUTHORS Brandt,M.E., Gabrik,A.H. and Vickery,L.E.
TITLE A vector for directional cloning and expression of polymerase chain
reaction products in Escherichia coli
JOURNAL Gene 97, 113-117 (1991)
STANDARD simple staff_entry
COMMENT
[Unpublished (1990)] sites.
[Unpublished (1990)] for [Unpublished (1990)].
Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by Vickery,L.E., 11-JUL-1990.
Author address: L.E.Vickery
Dept. Physiology and Biophysics
University of California
Irvine, CA 92717
FEATURES Location/Qualifiers
CDS 826..1446
/note="pfxblue fusion protein"
/codon_start=826
misc_recomb 918^919
/note="lambda cII peptide DNA end/factor Xa linker peptide
DNA start"
misc_recomb 939^940
/note="factor Xa linker peptide DNA end/modified lacZ'
gene DNA start"
BASE COUNT 956 a 911 c 860 g 900 t
ORIGIN
1 attatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa
61 catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt tgctgagttg
121 aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa gcaaaagttc
181 aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc cctcacttct
241 ggctggatga tggggcgatt caggctggta tgagtcagca acaccttctt cacgaggcag
301 acctcagcgc cggtgatgcc gacgatgcgt ccggcgtaga ggatctctca cctaccaaac
361 aatgcccccc tgcaaaaaat aaattcatat aaaaaacata cagataacca tctgcggtga
421 taaattatct ctggcggtgt tgacataaat accactggcg gtgatactga gcacatcagg
481 caggacgcac tgaccaccat gaaggtgacg ctcttaaaat taagccctga agaagggcag
541 cattcaaagc agaaggcttt ggggtgtgtg atacgaaacg aagcattggc gagctcgccc
601 ctaaagatca acaaggccga aagatttttt taactataaa cgctgatgga ggcgtttatg
661 cccaagaggt aaagcccttc ccgagtaaca aaaaaacaac agcataaata accccgctct
721 tacacattcc agccctgaaa aagggcatca aattaaacca cacctatggt gtatgcattt
781 atttgcatac attcaatcaa ttgttatcta aggaaatact tacatatggt tcgtgcaaac
841 aaacgcaacg aggctctacg aatcgagagt gcgttgctta acaaaatcgc aatgcttgga
901 actgagaaga cagcggaagg gggatccatc gagggtaggc ctaccatggt tacgaattcg
961 agctcggtac ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggcactggcc
1021 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
1081 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
1141 caacagttgc gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg
1201 gtgccggaaa gctggctgga gtgcgatctt cctgaggccg atacggtcgt cgtcccctca
1261 aactggcaga tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg
1321 gtcaatccgc cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat
1381 gttgatgaaa gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttcctatt
1441 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
1501 ttacaattta aatatttgct tatacaatct tcctgttttt ggggcttttc tgattatcaa
1561 ccgcggtaca tatgatggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
1621 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
1681 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
1741 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
1801 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
1861 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
1921 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
1981 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
2041 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
2101 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
2161 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
2221 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
2281 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
2341 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
2401 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
2461 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
2521 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
2581 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
2641 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
2701 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
2761 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
2821 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
2881 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
2941 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
3001 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
3061 ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
3121 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
3181 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
3241 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
3301 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
3361 cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
3421 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
3481 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
3541 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
3601 gaatactcat actcttcctt tttcaat
//