GenBank-Updates@genbank.bio.net (03/20/91)
LOCUS LBALAF 874 bp ds-DNA BCT 20-MAR-1991
DEFINITION Lactobacillus acidophilus lactacin F (laf) gene, complete cds.
ACCESSION M57961
KEYWORDS lactacin F.
SOURCE Lactobacillus acidophilus (strain VPI11088, ATCC 11506, NCK88) DNA.
ORGANISM Lactobacillus acidophilus
Prokaryota; Bacteria; Firmicutes; Regular asporogenous rods;
Lactobacillaceae.
REFERENCE 1 (bases 1 to 874)
AUTHORS Muriana,P.M. and Klaenhammer,T.R.
TITLE Cloning phenotypic expression, and DNA sequence of the gene for
lactacin F, an antimicrobial peptide produced by Latobacillus spp.
JOURNAL J. Bacteriol. 173, 1779-1788 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
-35_signal 203..209
/gene="laf"
-10_signal 228..233
/gene="laf"
RBS 283..290
/gene="laf"
CDS 299..526
/note="The N terminal amino acid of th active peptide was
determined to be Arg (nt 353) by amino acid sequencing.
The 18 amino acid upstream region is not present in the
bactericidal peptide"
/product="lactacin F"
/gene="laf"
/codon_start=299
sig_peptide 299..352
/gene="laf"
/codon_start=299
mat_peptide 353..523
/function="active peptide"
/product="lactacin F"
/gene="laf"
/evidence=EXPERIMENTAL
/codon_start=353
BASE COUNT 305 a 93 c 199 g 277 t
ORIGIN
1 cttggagtct atcctgtgaa catgaataaa gcaaaatata tacgaaatta tttcgactgc
61 aaaagttaat gatgagaata ttaaatatgg gatgcagggg aatgtgacga taatgaccgg
121 taggagtacg tatttaaaat atcttttgga taaggtaagg aataataagt aaaaaatact
181 aatttagtta ataaaagtaa ttttagacac aaatagaaca atattggtca attttatatc
241 ttaagatgat aactttagta agctatgcat ataaataaaa ttttaggagg tttctatcat
301 gaaacaattt aattatttat cacataaaga tttagcagtc gttgttggtg gaagaaataa
361 ttggcaaaca aatgtgggag gagcagtggg atcagctatg attggggcta cagttggtgg
421 tacaatttgt ggacctgcat gtgctgtagc tggtgcccat tatcttccta ttttatggac
481 aggggttaca gctgcaacag gtggttttgg caagataaga aagtaggatt ttgacaatga
541 aattaaatga caaagaatta tcaaagattg ttggtggaaa tcgatgggga gatactgttt
601 tatcagctgc tagtgggcgc aggaactggt attaaagcat gtaaaagttt tggcccatgg
661 ggaatggcaa tttgtggtgt aggaggtgca gcaataggag gttattttgg ctatactcat
721 aattaaacta tagtcaatta aagtaaaaca gtgatgattt gatatttagc actgcattac
781 tttattcata gattcattag taggtagttt aaatgactaa acattataaa attattggtc
841 taaggatatg tcatgggtaa ttacgattac tgga
//