GenBank-Updates@genbank.bio.net (02/19/91)
LOCUS PSEPHABC 6455 bp ds-DNA BCT 19-FEB-1991
DEFINITION P.oleovorans poly(3-hydroxyalkanoates) polyemrase 1 (phaA),
depolymerase (phaB) and polymerase 2 (phaC) genes, complete cds.
ACCESSION M58445 J05740
KEYWORDS PHA-depolymerase; PHA-polymerase 1; PHA-polymerase 2;
poly(3-hydroxyalkanoates).
SOURCE P.oleovorans DNA.
ORGANISM Pseudomonas oleovorans
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (bases 1 to 6455)
AUTHORS Huisman,G.W., Wonink,E., Meima,R., Kazemier,B., Terpstra,P. and
Witholt,B.
TITLE Metabolism of poly(3-hydroxyalkanoates) by Pseudomonas oleovorans:
Identification and sequences of genes and function of the encoded
proteins in the synthesis and degradation of PHA
JOURNAL J. Biol. Chem. (1990) In press
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 554..2233
/product="PHA-polymerase 1"
/gene="phaA"
/evidence=EXPERIMENTAL
/codon_start=554
CDS 2297..3148
/product="PHA-depolymerase"
/gene="phaB"
/evidence=EXPERIMENTAL
/codon_start=2297
CDS 3268..4950
/product="PHA-polymerase 2"
/gene="phaC"
/evidence=EXPERIMENTAL
/codon_start=3268
BASE COUNT 1218 a 2185 c 1950 g 1102 t
ORIGIN
1 gaattcctgc gcgtgcactc cccctccgcc gaggtccagg gccacggtaa ccccatcctg
61 cagttcggca agatcaacgt cggcctcagc ggcctggaac ctgccgggca atacgcactg
121 aaactgacct tcgacgacgg ccatgacagc ggcctgttca cctgggaata cctcgagcag
181 ctgtgcctgc gccaggaaca gctgtgggcc gagtacctcg acgaactgca caaggccggg
241 aaatcccgcg accctgccga gtcggtggtc aaactcatgc tctagcgcaa ggcctgcagg
301 atttagagcg cattttctaa aatcatctgt ttgaatgact tacagacagc ccagtgacgg
361 gctgtcttgc gcattacatg aaagtcgggt aaccaatggg ggtggcaagt tccctgcatc
421 aaattgcagg tagtcagaac cctcgcagca ccgctgttcc ttatcactgg tcacccgagt
481 agcagtaccg ggctcagaac tgtgcacccg ccacagcaac cggtactcgt ctcaggacaa
541 cggagcgtcg tagatgagta acaagaacaa cgatgagctg cagcggcagg cctcggaaaa
601 caccctgggg ctgaacccgg tcatcggtat ccgccgcaaa gacctgttga gctcggcacg
661 caccgtgctg cgccaggccg tgcgccaacc gctgcacagc gccaagcatg tggcccactt
721 tggcctggag ctgaagaacg tgctgctggg caagtccagc cttgccccgg aaagcgacga
781 ccgtcgcttc aatgacccgg catggagcaa caacccactt taccgccgct acctgcaaac
841 ctatctggcc tggcgcaagg agctgcagga ctggatcggc aacagcgacc tgtcgcccca
901 ggacatcagc cgcggccagt tcgtcatcaa cctgatgacc gaagccatgg ctccgaccaa
961 caccctgtcc aacccggcag cagtcaaacg cttcttcgaa accggcggca agagcctgct
1021 cgatggcctg tccaacctgg ccaaggacct ggtcaacaac ggtggcatgc ccagccaggt
1081 gaacatggac gccttcgagg tgggcaagaa cctgggcacc agtgaaggcg ccgtggtgta
1141 ccgcaacgat gtgctggagc tgatccagta caagcccatc accgagcagg tgcatgcccg
1201 cccgctgctg gtggtgccgc cgcagatcaa caagttctac gtattcgacc tgagcccgga
1261 aaagagcctg gcacgctact gcctgcgctc gcagcagcag accttcatca tcagctggcg
1321 caacccgacc aaagcccagc gcgaatgggg cctgtccacc tacatcgacg cgctcaagga
1381 ggcggtcgac gcggtgctgg cgattaccgg cagcaaggac ctgaacatgc tcggtgcctg
1441 ctccggcggc atcacctgca cggcattggt cggccactat gccgccctcg gcgaaaacaa
1501 ggtcaatgcc ctgaccctgc tggtcagcgt gctggacacc accatggaca accaggtcgc
1561 cctgttcgtc gacgagcaga ctttggaggc cgccaagcgc cactcctacc aggccggtgt
1621 gctcgaaggc agcgagatgg ccaaggtgtt cgcctggatg cgccccaacg acctgatctg
1681 gaactactgg gtcaacaact acctgctcgg caacgagccg ccggtgttcg acatcctgtt
1741 ctggaacaac gacaccacgc gcctgccggc cgccttccac ggcgacctga tcgaaatgtt
1801 caagagcaac ccgctgaccc gcccggacgc cctggaggtt tgcggcactc cgatcgacct
1861 gaaacaggtc aaatgcgaca tctacagcct tgccggcacc aacgaccaca tcaccccgtg
1921 gcagtcatgc taccgctcgg cgcacctgtt cggcggcaag atcgagttcg tgctgtccaa
1981 cagcggccac atccagagca tcctcaaccc gccaggcaac cccaaggcgc gcttcatgac
2041 cggtgccgat cgcccgggtg acccggtggc ctggcaggaa aacgccacca agcatgccga
2101 ctcctggtgg ctgcactggc aaagctggct gggcgagcgt gccggcgagc tggaaaaggc
2161 gccgacccgc ctgggcaacc gtgcctatgc cgctggcgag gcatccccgg gcacctacgt
2221 tcacgagcgt tgagctgcag cgccgtggcc acctgcggga cgccacggtg ttcatttcac
2281 cccatgagtc acgcgcatgc cgcaacccta catcttcagg accgtcgagc tggacaacca
2341 gtccatccgc accgccgtcc gcccgggcaa accgcacctg acgccgttgc tgatcttcaa
2401 cggcatcggt gccaacctgg agctggtgtt tccgttcatc gaggcactgg acccggacct
2461 ggaagtcatt gcctttgacg tacccggggt cggcggctcg tccacgccgc gccacccata
2521 ccgcttcccc gggttggcca agctgacggc acgcatgctc gactacctcg actacggcca
2581 ggtcaatgtc atcggtgtgt cttggggcgg cgccctggcc cagcagttcg cccacgatta
2641 ccccgaacgc tgcaagaaac tggtgctggc cgccaccgca gccggtgcgg tgatggtgcc
2701 aggcaagccc aaggtgttgt ggatgatggc cagcccacgg cgttacgtgc agccgtcgca
2761 tgtcatccgc attgcgccga cgatctatgg cggcggcttc cggcgtgacc ccgaactggc
2821 catgcagcac gcctccaagg tgcgctccgg cggcaagatg ggctactact ggcagctgtt
2881 cgccgggctc ggctggacca gcatccactg gctgcacaag atccagcaac cgaccctggt
2941 gctggccggc gacgacgacc cgctgatccc gctgatcaac atgcgcctgc tggcctggcg
3001 gattcccaat gcccagctac acattatcga cgacggtcat ttgttcctga tcacccgggc
3061 cgaggccgtc gccccgatca tcatgaagtt ccttcagcaa gaacgacagc gcgccgtcat
3121 gcaccctcgc ccggcttcgg gcgggtaaat cgatgcggcc ttcttcgcgg gcgcgcccgc
3181 tcccacaggg atggcgccga acctgtggga gcgggcatgc ccgcgaaggt ctcgacagcg
3241 aaatggctta gacgagggag tgttgccatg aaagacaaac cggccaaagg aacgccaacg
3301 cttcccgcca ccagcatgaa cgtgcagaac gccatcctcg gcctgcgcgg tcgtgacctg
3361 atttccacgc tgcgcaatgt cagccgccaa agcctgcgtc acccgctgca caccgcacat
3421 cacctgttgg ccctgggtgg ccagctgggc cgggtgatac tgggtgacac accgcttcag
3481 ccgaacccgc gcgatccgcg cttcagcgac ccgacatgga gccagaaccc gttctaccgg
3541 cgcggcctgc aagcctacct ggcctggcag aagcagaccc gcctgtggat cgaggaaagc
3601 cacctggacg acgatgaccg ggcccgtgcg cacttcctgt tcaacctgat caacgatgcc
3661 ctggcgccaa gcaactcgct gctcaacccg ctggcggtca aggaactgtt caacagcggt
3721 ggccagagcc tggtgcgcgg cgtggcccac ctgctcgatg acctgcgcca caatgacggc
3781 ctgccacgcc aggtcgacga gcgcgccttc gaagtgggcg gcaacctggc cgcgactgcc
3841 ggcgccgtgg tgtttcgcaa cgagctgctg gaactgatcc agtacaagcc gatgagcgaa
3901 aagcagcacg cccggccact gctggtggtg ccgccacaga tcaacaagtt ctacatcttc
3961 gacctcagct cgaccaacag cttcgtccag tacatgctca agaatggcct gcaggtgttc
4021 atggtcagct ggcgcaaccc cgacccgcgc caccgcgaat ggggcctgtc cagctacgtg
4081 caggccctgg aagaagcgct caacgcttgc cgcagcatta gcggcaaccg cgaccccaac
4141 ctgatgggcg cctgcgccgg cggcctgacc atggccgcac tgcagggcca cctgcaggcc
4201 aagcaccagc tgcgccgggt gcgcagcgcc acctacctgg tcagcttgct ggacagcaag
4261 ttcgaaagcc ccgccagcct gttcgccgac gagcagacca tcgaggccgc caagcgccgc
4321 tcctaccagc gcggtgtgct ggatggcgcc gaggtggcgc ggatcttcgc ctggatgcgg
4381 cccaacgacc tgatctggaa ctactgggtc aacaactacc tgctcggcaa gacaccacca
4441 gccttcgaca tcctgtactg gaacgccgac agcacgcgcc tgcccgccgc gctgcatggc
4501 gacctgctgg acttcttcaa gctcaacccg ctgacccacc cagccggcct ggaggtatgc
4561 ggcacaccca tcgacctgca gaaggtcgag ctggacagtt tcaccgtggc cggcagcaac
4621 gaccacatca ccccgtggga tgcggtgtac cgctcggcct tgctgctggg tggcgaccgg
4681 cgcttcgtgc tggccaacag cgggcacatc cagagcatca tcaacccgcc cggcaacccc
4741 aaggcctact acctggccaa ccccaagctg tccagcgacc cgcgtgcctg gctccacgat
4801 gccaagcgca gcgaaggcag ctggtggccg ttgtggctgg agtggatcac cgcgcgctcc
4861 ggcccgctca aggcaccgcg cagcgaactg ggcaatgcca cctacccacc gctgggcccc
4921 gcgccgggca cctacgtgct gacccgatga gcatgccgac tggatgaaga ctcgcgaccg
4981 tatcctcgaa tgtgccctgc agctgttcaa ccagcagggc gaaccgaacg tatccaccct
5041 ggaaattgcc aacgaactgg gcatcagccc tggcaacctc tactaccact tccacggcaa
5101 ggagccgttg gtgctggggt tgttcgagcg ctttgaagaa gcgctgatgc ccttgctcga
5161 cccgccgctg gaggtacgcc tggacgccga ggattactgg ctgttcctgc acctgatcgt
5221 cgaacgcatg gcgcagtacc gcttcctgtt ccaggacctg tcgaacctga ccgggcgcct
5281 gcccaaactg gcccgcggca tgcgcaacct gatcaacgcg ctcaagcgca cactggcggc
5341 gttgctggcc agcctcaagg gccaagggtt ggtagagagc gagacccagg cgctggggca
5401 actggtggag cagatcaccc tgacactgat gttctcgctg gattatcagc gggtactggg
5461 gcgcgagggg gatgtgggga ttgtggtgta ccaggtgatg atgctggtgg cgccgcatct
5521 gcaggcccag gcgcgggggg cggcggagca attggcggtg cggtatctgg aggggtaagc
5581 ctgttgattc ggtgtcgcgg ctttcgcggg catgcccgct cccacaggtg aaatgcagtg
5641 ctcgagtgca cacaggacct gtgggagcgg gcaagcccgc gaagatggcg acgcggtatc
5701 agatcagggt accggtgcct gtctgtgccg aaggcgggtt gctggccgga gccgcagtgg
5761 gcgccgaagc tgcgctagcg gccggagcgg cgctggccgc tggagctgcc ggcttggcgg
5821 ctgccggctt ggccggtgct ttcttcactg caggcttctt cgccacagcc ggtttggccg
5881 ctgccggttt gccgcaggct tggccgccgc agtcttggca gcaggtttag ccgctgcggc
5941 cttggctgcc ggcttggctg ccgcggtttt gccgcaggct tggccgctgc cgtcttggcc
6001 agtggcttgg ccgccgcctt gctcgcagcc ggtttggctg cagtgcgcga cgaaatcggg
6061 gtaaccgaag cgccggtgag tttctcgatc tgcttggtca ggctgtccac ctgctggtgc
6121 agggccttga tctcgttgcg gctcggcacg ccaaggcgcg agatggcact gttcaggcgc
6181 ttgtcgaagg cctcttcgag ttcgctccac ttgcctagcg cacggtcctt cacgcccgac
6241 acacgcgaag tggtcgacga cttggcagtt tcagcaacat cttctgcggt cttcttcgcc
6301 tgtttctcgg ccttctcgcc atcctttacc agcgagtcga acagcttcgg gccgtcctgg
6361 tcgatcttcg aatagatacc aagccccgcc agccagatct tgcgggagta cttctcgatc
6421 ccgccgccca ggagctgcct tctttctcgg aattc
//GenBank-Updates@genbank.bio.net (03/07/91)
LOCUS PSEPHABC 6455 bp ds-DNA BCT 07-MAR-1991
DEFINITION P.oleovorans poly(3-hydroxyalkanoates) polyemrase 1 (phaA),
depolymerase (phaB) and polymerase 2 (phaC) genes, complete cds.
ACCESSION M58445 J05740
KEYWORDS PHA-depolymerase; PHA-polymerase 1; PHA-polymerase 2;
poly(3-hydroxyalkanoates).
SOURCE P.oleovorans DNA.
ORGANISM Pseudomonas oleovorans
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (bases 1 to 6455)
AUTHORS Huisman,G.W., Wonink,E., Meima,R., Kazemier,B., Terpstra,P. and
Witholt,B.
TITLE Metabolism of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas
oleovorans: Identification and sequences of genes and function of
the encoded proteins in the synthesis and degradation of PHA
JOURNAL J. Biol. Chem. 266, 2191-2198 (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 554..2233
/product="PHA-polymerase 1"
/gene="phaA"
/evidence=EXPERIMENTAL
/codon_start=554
CDS 2297..3148
/product="PHA-depolymerase"
/gene="phaB"
/evidence=EXPERIMENTAL
/codon_start=2297
CDS 3268..4950
/product="PHA-polymerase 2"
/gene="phaC"
/evidence=EXPERIMENTAL
/codon_start=3268
BASE COUNT 1218 a 2185 c 1950 g 1102 t
ORIGIN
1 gaattcctgc gcgtgcactc cccctccgcc gaggtccagg gccacggtaa ccccatcctg
61 cagttcggca agatcaacgt cggcctcagc ggcctggaac ctgccgggca atacgcactg
121 aaactgacct tcgacgacgg ccatgacagc ggcctgttca cctgggaata cctcgagcag
181 ctgtgcctgc gccaggaaca gctgtgggcc gagtacctcg acgaactgca caaggccggg
241 aaatcccgcg accctgccga gtcggtggtc aaactcatgc tctagcgcaa ggcctgcagg
301 atttagagcg cattttctaa aatcatctgt ttgaatgact tacagacagc ccagtgacgg
361 gctgtcttgc gcattacatg aaagtcgggt aaccaatggg ggtggcaagt tccctgcatc
421 aaattgcagg tagtcagaac cctcgcagca ccgctgttcc ttatcactgg tcacccgagt
481 agcagtaccg ggctcagaac tgtgcacccg ccacagcaac cggtactcgt ctcaggacaa
541 cggagcgtcg tagatgagta acaagaacaa cgatgagctg cagcggcagg cctcggaaaa
601 caccctgggg ctgaacccgg tcatcggtat ccgccgcaaa gacctgttga gctcggcacg
661 caccgtgctg cgccaggccg tgcgccaacc gctgcacagc gccaagcatg tggcccactt
721 tggcctggag ctgaagaacg tgctgctggg caagtccagc cttgccccgg aaagcgacga
781 ccgtcgcttc aatgacccgg catggagcaa caacccactt taccgccgct acctgcaaac
841 ctatctggcc tggcgcaagg agctgcagga ctggatcggc aacagcgacc tgtcgcccca
901 ggacatcagc cgcggccagt tcgtcatcaa cctgatgacc gaagccatgg ctccgaccaa
961 caccctgtcc aacccggcag cagtcaaacg cttcttcgaa accggcggca agagcctgct
1021 cgatggcctg tccaacctgg ccaaggacct ggtcaacaac ggtggcatgc ccagccaggt
1081 gaacatggac gccttcgagg tgggcaagaa cctgggcacc agtgaaggcg ccgtggtgta
1141 ccgcaacgat gtgctggagc tgatccagta caagcccatc accgagcagg tgcatgcccg
1201 cccgctgctg gtggtgccgc cgcagatcaa caagttctac gtattcgacc tgagcccgga
1261 aaagagcctg gcacgctact gcctgcgctc gcagcagcag accttcatca tcagctggcg
1321 caacccgacc aaagcccagc gcgaatgggg cctgtccacc tacatcgacg cgctcaagga
1381 ggcggtcgac gcggtgctgg cgattaccgg cagcaaggac ctgaacatgc tcggtgcctg
1441 ctccggcggc atcacctgca cggcattggt cggccactat gccgccctcg gcgaaaacaa
1501 ggtcaatgcc ctgaccctgc tggtcagcgt gctggacacc accatggaca accaggtcgc
1561 cctgttcgtc gacgagcaga ctttggaggc cgccaagcgc cactcctacc aggccggtgt
1621 gctcgaaggc agcgagatgg ccaaggtgtt cgcctggatg cgccccaacg acctgatctg
1681 gaactactgg gtcaacaact acctgctcgg caacgagccg ccggtgttcg acatcctgtt
1741 ctggaacaac gacaccacgc gcctgccggc cgccttccac ggcgacctga tcgaaatgtt
1801 caagagcaac ccgctgaccc gcccggacgc cctggaggtt tgcggcactc cgatcgacct
1861 gaaacaggtc aaatgcgaca tctacagcct tgccggcacc aacgaccaca tcaccccgtg
1921 gcagtcatgc taccgctcgg cgcacctgtt cggcggcaag atcgagttcg tgctgtccaa
1981 cagcggccac atccagagca tcctcaaccc gccaggcaac cccaaggcgc gcttcatgac
2041 cggtgccgat cgcccgggtg acccggtggc ctggcaggaa aacgccacca agcatgccga
2101 ctcctggtgg ctgcactggc aaagctggct gggcgagcgt gccggcgagc tggaaaaggc
2161 gccgacccgc ctgggcaacc gtgcctatgc cgctggcgag gcatccccgg gcacctacgt
2221 tcacgagcgt tgagctgcag cgccgtggcc acctgcggga cgccacggtg ttcatttcac
2281 cccatgagtc acgcgcatgc cgcaacccta catcttcagg accgtcgagc tggacaacca
2341 gtccatccgc accgccgtcc gcccgggcaa accgcacctg acgccgttgc tgatcttcaa
2401 cggcatcggt gccaacctgg agctggtgtt tccgttcatc gaggcactgg acccggacct
2461 ggaagtcatt gcctttgacg tacccggggt cggcggctcg tccacgccgc gccacccata
2521 ccgcttcccc gggttggcca agctgacggc acgcatgctc gactacctcg actacggcca
2581 ggtcaatgtc atcggtgtgt cttggggcgg cgccctggcc cagcagttcg cccacgatta
2641 ccccgaacgc tgcaagaaac tggtgctggc cgccaccgca gccggtgcgg tgatggtgcc
2701 aggcaagccc aaggtgttgt ggatgatggc cagcccacgg cgttacgtgc agccgtcgca
2761 tgtcatccgc attgcgccga cgatctatgg cggcggcttc cggcgtgacc ccgaactggc
2821 catgcagcac gcctccaagg tgcgctccgg cggcaagatg ggctactact ggcagctgtt
2881 cgccgggctc ggctggacca gcatccactg gctgcacaag atccagcaac cgaccctggt
2941 gctggccggc gacgacgacc cgctgatccc gctgatcaac atgcgcctgc tggcctggcg
3001 gattcccaat gcccagctac acattatcga cgacggtcat ttgttcctga tcacccgggc
3061 cgaggccgtc gccccgatca tcatgaagtt ccttcagcaa gaacgacagc gcgccgtcat
3121 gcaccctcgc ccggcttcgg gcgggtaaat cgatgcggcc ttcttcgcgg gcgcgcccgc
3181 tcccacaggg atggcgccga acctgtggga gcgggcatgc ccgcgaaggt ctcgacagcg
3241 aaatggctta gacgagggag tgttgccatg aaagacaaac cggccaaagg aacgccaacg
3301 cttcccgcca ccagcatgaa cgtgcagaac gccatcctcg gcctgcgcgg tcgtgacctg
3361 atttccacgc tgcgcaatgt cagccgccaa agcctgcgtc acccgctgca caccgcacat
3421 cacctgttgg ccctgggtgg ccagctgggc cgggtgatac tgggtgacac accgcttcag
3481 ccgaacccgc gcgatccgcg cttcagcgac ccgacatgga gccagaaccc gttctaccgg
3541 cgcggcctgc aagcctacct ggcctggcag aagcagaccc gcctgtggat cgaggaaagc
3601 cacctggacg acgatgaccg ggcccgtgcg cacttcctgt tcaacctgat caacgatgcc
3661 ctggcgccaa gcaactcgct gctcaacccg ctggcggtca aggaactgtt caacagcggt
3721 ggccagagcc tggtgcgcgg cgtggcccac ctgctcgatg acctgcgcca caatgacggc
3781 ctgccacgcc aggtcgacga gcgcgccttc gaagtgggcg gcaacctggc cgcgactgcc
3841 ggcgccgtgg tgtttcgcaa cgagctgctg gaactgatcc agtacaagcc gatgagcgaa
3901 aagcagcacg cccggccact gctggtggtg ccgccacaga tcaacaagtt ctacatcttc
3961 gacctcagct cgaccaacag cttcgtccag tacatgctca agaatggcct gcaggtgttc
4021 atggtcagct ggcgcaaccc cgacccgcgc caccgcgaat ggggcctgtc cagctacgtg
4081 caggccctgg aagaagcgct caacgcttgc cgcagcatta gcggcaaccg cgaccccaac
4141 ctgatgggcg cctgcgccgg cggcctgacc atggccgcac tgcagggcca cctgcaggcc
4201 aagcaccagc tgcgccgggt gcgcagcgcc acctacctgg tcagcttgct ggacagcaag
4261 ttcgaaagcc ccgccagcct gttcgccgac gagcagacca tcgaggccgc caagcgccgc
4321 tcctaccagc gcggtgtgct ggatggcgcc gaggtggcgc ggatcttcgc ctggatgcgg
4381 cccaacgacc tgatctggaa ctactgggtc aacaactacc tgctcggcaa gacaccacca
4441 gccttcgaca tcctgtactg gaacgccgac agcacgcgcc tgcccgccgc gctgcatggc
4501 gacctgctgg acttcttcaa gctcaacccg ctgacccacc cagccggcct ggaggtatgc
4561 ggcacaccca tcgacctgca gaaggtcgag ctggacagtt tcaccgtggc cggcagcaac
4621 gaccacatca ccccgtggga tgcggtgtac cgctcggcct tgctgctggg tggcgaccgg
4681 cgcttcgtgc tggccaacag cgggcacatc cagagcatca tcaacccgcc cggcaacccc
4741 aaggcctact acctggccaa ccccaagctg tccagcgacc cgcgtgcctg gctccacgat
4801 gccaagcgca gcgaaggcag ctggtggccg ttgtggctgg agtggatcac cgcgcgctcc
4861 ggcccgctca aggcaccgcg cagcgaactg ggcaatgcca cctacccacc gctgggcccc
4921 gcgccgggca cctacgtgct gacccgatga gcatgccgac tggatgaaga ctcgcgaccg
4981 tatcctcgaa tgtgccctgc agctgttcaa ccagcagggc gaaccgaacg tatccaccct
5041 ggaaattgcc aacgaactgg gcatcagccc tggcaacctc tactaccact tccacggcaa
5101 ggagccgttg gtgctggggt tgttcgagcg ctttgaagaa gcgctgatgc ccttgctcga
5161 cccgccgctg gaggtacgcc tggacgccga ggattactgg ctgttcctgc acctgatcgt
5221 cgaacgcatg gcgcagtacc gcttcctgtt ccaggacctg tcgaacctga ccgggcgcct
5281 gcccaaactg gcccgcggca tgcgcaacct gatcaacgcg ctcaagcgca cactggcggc
5341 gttgctggcc agcctcaagg gccaagggtt ggtagagagc gagacccagg cgctggggca
5401 actggtggag cagatcaccc tgacactgat gttctcgctg gattatcagc gggtactggg
5461 gcgcgagggg gatgtgggga ttgtggtgta ccaggtgatg atgctggtgg cgccgcatct
5521 gcaggcccag gcgcgggggg cggcggagca attggcggtg cggtatctgg aggggtaagc
5581 ctgttgattc ggtgtcgcgg ctttcgcggg catgcccgct cccacaggtg aaatgcagtg
5641 ctcgagtgca cacaggacct gtgggagcgg gcaagcccgc gaagatggcg acgcggtatc
5701 agatcagggt accggtgcct gtctgtgccg aaggcgggtt gctggccgga gccgcagtgg
5761 gcgccgaagc tgcgctagcg gccggagcgg cgctggccgc tggagctgcc ggcttggcgg
5821 ctgccggctt ggccggtgct ttcttcactg caggcttctt cgccacagcc ggtttggccg
5881 ctgccggttt gccgcaggct tggccgccgc agtcttggca gcaggtttag ccgctgcggc
5941 cttggctgcc ggcttggctg ccgcggtttt gccgcaggct tggccgctgc cgtcttggcc
6001 agtggcttgg ccgccgcctt gctcgcagcc ggtttggctg cagtgcgcga cgaaatcggg
6061 gtaaccgaag cgccggtgag tttctcgatc tgcttggtca ggctgtccac ctgctggtgc
6121 agggccttga tctcgttgcg gctcggcacg ccaaggcgcg agatggcact gttcaggcgc
6181 ttgtcgaagg cctcttcgag ttcgctccac ttgcctagcg cacggtcctt cacgcccgac
6241 acacgcgaag tggtcgacga cttggcagtt tcagcaacat cttctgcggt cttcttcgcc
6301 tgtttctcgg ccttctcgcc atcctttacc agcgagtcga acagcttcgg gccgtcctgg
6361 tcgatcttcg aatagatacc aagccccgcc agccagatct tgcgggagta cttctcgatc
6421 ccgccgccca ggagctgcct tctttctcgg aattc
//GenBank-Updates@genbank.bio.net (03/20/91)
LOCUS PSEPHAABC 6455 bp ds-DNA BCT 20-MAR-1991
DEFINITION P.oleovorans poly(3-hydroxyalkanoate) polymerase 1 (phaA),
depolymerase (phaB) and polymerase 2 (phaC) genes, complete cds.
ACCESSION M58445 J05740
KEYWORDS PHA-depolymerase; PHA-polymerase 1; PHA-polymerase 2;
poly(3-hydroxyalkanoates).
SOURCE P.oleovorans DNA.
ORGANISM Pseudomonas oleovorans
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (bases 1 to 6455)
AUTHORS Huisman,G.W., Wonink,E., Meima,R., Kazemier,B., Terpstra,P. and
Witholt,B.
TITLE Metabolism of poly(3-hydroxyalkanoates) (PHAs) by Pseudomonas
oleovorans: Identification and sequences of genes and function of
the encoded proteins in the synthesis and degradation of PHA
JOURNAL J. Biol. Chem. 266, 2191-2198 (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 554..2233
/product="PHA-polymerase 1"
/gene="phaA"
/evidence=EXPERIMENTAL
/codon_start=554
CDS 2297..3148
/product="PHA-depolymerase"
/gene="phaB"
/evidence=EXPERIMENTAL
/codon_start=2297
CDS 3268..4950
/product="PHA-polymerase 2"
/gene="phaC"
/evidence=EXPERIMENTAL
/codon_start=3268
BASE COUNT 1218 a 2185 c 1950 g 1102 t
ORIGIN
1 gaattcctgc gcgtgcactc cccctccgcc gaggtccagg gccacggtaa ccccatcctg
61 cagttcggca agatcaacgt cggcctcagc ggcctggaac ctgccgggca atacgcactg
121 aaactgacct tcgacgacgg ccatgacagc ggcctgttca cctgggaata cctcgagcag
181 ctgtgcctgc gccaggaaca gctgtgggcc gagtacctcg acgaactgca caaggccggg
241 aaatcccgcg accctgccga gtcggtggtc aaactcatgc tctagcgcaa ggcctgcagg
301 atttagagcg cattttctaa aatcatctgt ttgaatgact tacagacagc ccagtgacgg
361 gctgtcttgc gcattacatg aaagtcgggt aaccaatggg ggtggcaagt tccctgcatc
421 aaattgcagg tagtcagaac cctcgcagca ccgctgttcc ttatcactgg tcacccgagt
481 agcagtaccg ggctcagaac tgtgcacccg ccacagcaac cggtactcgt ctcaggacaa
541 cggagcgtcg tagatgagta acaagaacaa cgatgagctg cagcggcagg cctcggaaaa
601 caccctgggg ctgaacccgg tcatcggtat ccgccgcaaa gacctgttga gctcggcacg
661 caccgtgctg cgccaggccg tgcgccaacc gctgcacagc gccaagcatg tggcccactt
721 tggcctggag ctgaagaacg tgctgctggg caagtccagc cttgccccgg aaagcgacga
781 ccgtcgcttc aatgacccgg catggagcaa caacccactt taccgccgct acctgcaaac
841 ctatctggcc tggcgcaagg agctgcagga ctggatcggc aacagcgacc tgtcgcccca
901 ggacatcagc cgcggccagt tcgtcatcaa cctgatgacc gaagccatgg ctccgaccaa
961 caccctgtcc aacccggcag cagtcaaacg cttcttcgaa accggcggca agagcctgct
1021 cgatggcctg tccaacctgg ccaaggacct ggtcaacaac ggtggcatgc ccagccaggt
1081 gaacatggac gccttcgagg tgggcaagaa cctgggcacc agtgaaggcg ccgtggtgta
1141 ccgcaacgat gtgctggagc tgatccagta caagcccatc accgagcagg tgcatgcccg
1201 cccgctgctg gtggtgccgc cgcagatcaa caagttctac gtattcgacc tgagcccgga
1261 aaagagcctg gcacgctact gcctgcgctc gcagcagcag accttcatca tcagctggcg
1321 caacccgacc aaagcccagc gcgaatgggg cctgtccacc tacatcgacg cgctcaagga
1381 ggcggtcgac gcggtgctgg cgattaccgg cagcaaggac ctgaacatgc tcggtgcctg
1441 ctccggcggc atcacctgca cggcattggt cggccactat gccgccctcg gcgaaaacaa
1501 ggtcaatgcc ctgaccctgc tggtcagcgt gctggacacc accatggaca accaggtcgc
1561 cctgttcgtc gacgagcaga ctttggaggc cgccaagcgc cactcctacc aggccggtgt
1621 gctcgaaggc agcgagatgg ccaaggtgtt cgcctggatg cgccccaacg acctgatctg
1681 gaactactgg gtcaacaact acctgctcgg caacgagccg ccggtgttcg acatcctgtt
1741 ctggaacaac gacaccacgc gcctgccggc cgccttccac ggcgacctga tcgaaatgtt
1801 caagagcaac ccgctgaccc gcccggacgc cctggaggtt tgcggcactc cgatcgacct
1861 gaaacaggtc aaatgcgaca tctacagcct tgccggcacc aacgaccaca tcaccccgtg
1921 gcagtcatgc taccgctcgg cgcacctgtt cggcggcaag atcgagttcg tgctgtccaa
1981 cagcggccac atccagagca tcctcaaccc gccaggcaac cccaaggcgc gcttcatgac
2041 cggtgccgat cgcccgggtg acccggtggc ctggcaggaa aacgccacca agcatgccga
2101 ctcctggtgg ctgcactggc aaagctggct gggcgagcgt gccggcgagc tggaaaaggc
2161 gccgacccgc ctgggcaacc gtgcctatgc cgctggcgag gcatccccgg gcacctacgt
2221 tcacgagcgt tgagctgcag cgccgtggcc acctgcggga cgccacggtg ttcatttcac
2281 cccatgagtc acgcgcatgc cgcaacccta catcttcagg accgtcgagc tggacaacca
2341 gtccatccgc accgccgtcc gcccgggcaa accgcacctg acgccgttgc tgatcttcaa
2401 cggcatcggt gccaacctgg agctggtgtt tccgttcatc gaggcactgg acccggacct
2461 ggaagtcatt gcctttgacg tacccggggt cggcggctcg tccacgccgc gccacccata
2521 ccgcttcccc gggttggcca agctgacggc acgcatgctc gactacctcg actacggcca
2581 ggtcaatgtc atcggtgtgt cttggggcgg cgccctggcc cagcagttcg cccacgatta
2641 ccccgaacgc tgcaagaaac tggtgctggc cgccaccgca gccggtgcgg tgatggtgcc
2701 aggcaagccc aaggtgttgt ggatgatggc cagcccacgg cgttacgtgc agccgtcgca
2761 tgtcatccgc attgcgccga cgatctatgg cggcggcttc cggcgtgacc ccgaactggc
2821 catgcagcac gcctccaagg tgcgctccgg cggcaagatg ggctactact ggcagctgtt
2881 cgccgggctc ggctggacca gcatccactg gctgcacaag atccagcaac cgaccctggt
2941 gctggccggc gacgacgacc cgctgatccc gctgatcaac atgcgcctgc tggcctggcg
3001 gattcccaat gcccagctac acattatcga cgacggtcat ttgttcctga tcacccgggc
3061 cgaggccgtc gccccgatca tcatgaagtt ccttcagcaa gaacgacagc gcgccgtcat
3121 gcaccctcgc ccggcttcgg gcgggtaaat cgatgcggcc ttcttcgcgg gcgcgcccgc
3181 tcccacaggg atggcgccga acctgtggga gcgggcatgc ccgcgaaggt ctcgacagcg
3241 aaatggctta gacgagggag tgttgccatg aaagacaaac cggccaaagg aacgccaacg
3301 cttcccgcca ccagcatgaa cgtgcagaac gccatcctcg gcctgcgcgg tcgtgacctg
3361 atttccacgc tgcgcaatgt cagccgccaa agcctgcgtc acccgctgca caccgcacat
3421 cacctgttgg ccctgggtgg ccagctgggc cgggtgatac tgggtgacac accgcttcag
3481 ccgaacccgc gcgatccgcg cttcagcgac ccgacatgga gccagaaccc gttctaccgg
3541 cgcggcctgc aagcctacct ggcctggcag aagcagaccc gcctgtggat cgaggaaagc
3601 cacctggacg acgatgaccg ggcccgtgcg cacttcctgt tcaacctgat caacgatgcc
3661 ctggcgccaa gcaactcgct gctcaacccg ctggcggtca aggaactgtt caacagcggt
3721 ggccagagcc tggtgcgcgg cgtggcccac ctgctcgatg acctgcgcca caatgacggc
3781 ctgccacgcc aggtcgacga gcgcgccttc gaagtgggcg gcaacctggc cgcgactgcc
3841 ggcgccgtgg tgtttcgcaa cgagctgctg gaactgatcc agtacaagcc gatgagcgaa
3901 aagcagcacg cccggccact gctggtggtg ccgccacaga tcaacaagtt ctacatcttc
3961 gacctcagct cgaccaacag cttcgtccag tacatgctca agaatggcct gcaggtgttc
4021 atggtcagct ggcgcaaccc cgacccgcgc caccgcgaat ggggcctgtc cagctacgtg
4081 caggccctgg aagaagcgct caacgcttgc cgcagcatta gcggcaaccg cgaccccaac
4141 ctgatgggcg cctgcgccgg cggcctgacc atggccgcac tgcagggcca cctgcaggcc
4201 aagcaccagc tgcgccgggt gcgcagcgcc acctacctgg tcagcttgct ggacagcaag
4261 ttcgaaagcc ccgccagcct gttcgccgac gagcagacca tcgaggccgc caagcgccgc
4321 tcctaccagc gcggtgtgct ggatggcgcc gaggtggcgc ggatcttcgc ctggatgcgg
4381 cccaacgacc tgatctggaa ctactgggtc aacaactacc tgctcggcaa gacaccacca
4441 gccttcgaca tcctgtactg gaacgccgac agcacgcgcc tgcccgccgc gctgcatggc
4501 gacctgctgg acttcttcaa gctcaacccg ctgacccacc cagccggcct ggaggtatgc
4561 ggcacaccca tcgacctgca gaaggtcgag ctggacagtt tcaccgtggc cggcagcaac
4621 gaccacatca ccccgtggga tgcggtgtac cgctcggcct tgctgctggg tggcgaccgg
4681 cgcttcgtgc tggccaacag cgggcacatc cagagcatca tcaacccgcc cggcaacccc
4741 aaggcctact acctggccaa ccccaagctg tccagcgacc cgcgtgcctg gctccacgat
4801 gccaagcgca gcgaaggcag ctggtggccg ttgtggctgg agtggatcac cgcgcgctcc
4861 ggcccgctca aggcaccgcg cagcgaactg ggcaatgcca cctacccacc gctgggcccc
4921 gcgccgggca cctacgtgct gacccgatga gcatgccgac tggatgaaga ctcgcgaccg
4981 tatcctcgaa tgtgccctgc agctgttcaa ccagcagggc gaaccgaacg tatccaccct
5041 ggaaattgcc aacgaactgg gcatcagccc tggcaacctc tactaccact tccacggcaa
5101 ggagccgttg gtgctggggt tgttcgagcg ctttgaagaa gcgctgatgc ccttgctcga
5161 cccgccgctg gaggtacgcc tggacgccga ggattactgg ctgttcctgc acctgatcgt
5221 cgaacgcatg gcgcagtacc gcttcctgtt ccaggacctg tcgaacctga ccgggcgcct
5281 gcccaaactg gcccgcggca tgcgcaacct gatcaacgcg ctcaagcgca cactggcggc
5341 gttgctggcc agcctcaagg gccaagggtt ggtagagagc gagacccagg cgctggggca
5401 actggtggag cagatcaccc tgacactgat gttctcgctg gattatcagc gggtactggg
5461 gcgcgagggg gatgtgggga ttgtggtgta ccaggtgatg atgctggtgg cgccgcatct
5521 gcaggcccag gcgcgggggg cggcggagca attggcggtg cggtatctgg aggggtaagc
5581 ctgttgattc ggtgtcgcgg ctttcgcggg catgcccgct cccacaggtg aaatgcagtg
5641 ctcgagtgca cacaggacct gtgggagcgg gcaagcccgc gaagatggcg acgcggtatc
5701 agatcagggt accggtgcct gtctgtgccg aaggcgggtt gctggccgga gccgcagtgg
5761 gcgccgaagc tgcgctagcg gccggagcgg cgctggccgc tggagctgcc ggcttggcgg
5821 ctgccggctt ggccggtgct ttcttcactg caggcttctt cgccacagcc ggtttggccg
5881 ctgccggttt gccgcaggct tggccgccgc agtcttggca gcaggtttag ccgctgcggc
5941 cttggctgcc ggcttggctg ccgcggtttt gccgcaggct tggccgctgc cgtcttggcc
6001 agtggcttgg ccgccgcctt gctcgcagcc ggtttggctg cagtgcgcga cgaaatcggg
6061 gtaaccgaag cgccggtgag tttctcgatc tgcttggtca ggctgtccac ctgctggtgc
6121 agggccttga tctcgttgcg gctcggcacg ccaaggcgcg agatggcact gttcaggcgc
6181 ttgtcgaagg cctcttcgag ttcgctccac ttgcctagcg cacggtcctt cacgcccgac
6241 acacgcgaag tggtcgacga cttggcagtt tcagcaacat cttctgcggt cttcttcgcc
6301 tgtttctcgg ccttctcgcc atcctttacc agcgagtcga acagcttcgg gccgtcctgg
6361 tcgatcttcg aatagatacc aagccccgcc agccagatct tgcgggagta cttctcgatc
6421 ccgccgccca ggagctgcct tctttctcgg aattc
//