[bionet.molbio.genbank.updates] E.coli adenosine deaminase

GenBank-Updates@genbank.bio.net (02/22/91)

LOCUS       ECOADD       1200 bp ds-DNA             BCT       22-FEB-1991
DEFINITION  E.coli adenosine deaminase (add) gene, complete cds.
ACCESSION   M59033 J05320
KEYWORDS    adenosine deaminase.
SOURCE      E.coli K-12 DNA.
  ORGANISM  Escherichia coli
            Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively
            anaerobic rods; Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Chang,Z., Nygaard,P. and Kellems,R.E.
  TITLE     The deduced amino acid sequence of E.coli adenosine deaminase
            reveals evolutionarily conserved amino acid residues: Implications
            for catalytic function
  JOURNAL   Biochemistry (1990) In press
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     -35_signal      49..54
                     /note="consensus"
                     /gene="add"
     -10_signal      67..72
                     /note="consensus"
                     /gene="add"
     misc_feature    67..88
                     /note="operator"
                     /gene="add"
     mRNA            78..1116
                     /product="adenosine deaminase"
                     /gene="add"
     mRNA            80..1116
                     /product="adenosine deaminase"
                     /gene="add"
     RBS             99..103
                     /gene="add"
     CDS             109..1107
                     /EC_number="3.5.4.4"
                     /product="adenosine deaminase"
                     /gene="add"
                     /codon_start=109
     terminator      1116..1139
                     /gene="add"
BASE COUNT      282 a    321 c    305 g    292 t
ORIGIN
        1 cgtcgttaac cccaattcgc aagtaaaaaa tcgttgcaat cgtggatttt taccctgctt
       61 tgtttttata atggtgcgca cttttatatc cagaaaaaga gtgcgaccat gattgatacc
      121 accctgccat taactgatat ccatcgccac cttgatggca acattcgtcc ccagaccatt
      181 cttgaacttg gccgccagta taatatctcg cttcctgcac aatccctgga aacactgatt
      241 ccccacgttc aggtcattgc caacgaaccc gatctggtga gctttctgac caaacttgac
      301 tggggcgtta aagttctcgc ctctcttgat gcctgtcgcc gcgtggcatt tgaaaacatt
      361 gaagatgcag cccgtcacgg cctgcactat gtcgagctgc gtttttcacc aggctacatg
      421 gcaatggcac atcagctgcc tgtagcgggt gttgtcgaag cggtgatcga tggcgtacgt
      481 gaaggttgcc gcacctttgg tgtgcaggcg aagcttatcg gcattatgag ccggaccttc
      541 gaagccgcct gtcagcaaga gctggaggcc tttttagccc accgtgacca gattaccgca
      601 cttgatttag ccggtgatga acttggtttc ccgggaagtc tgttcctttc tcacttcaac
      661 cgcgcgcgtg atgcgggctg gcatattacc gtccatgcag gcgaagctgc cgggccggaa
      721 agcatctggc aggcgattcg tgaactgggt gcggagcgta ttggacatgg cgtaaaagcc
      781 attgaagatc gggcgctgat ggattttctc gccgagcaac aaattggtat tgaatcctgt
      841 ctgacctcca atattcagac cagcaccgta gcagagctgg ctgcacatcc gctgaaaacg
      901 ttccttgagc atggcattcg tgccagcatt aacactgacg atcccggcgt acagggagtg
      961 gatatcattc acgaatatac cgttgccgcg ccagctgctg ggttatcccg cgagcaaatc
     1021 cgccaggcac agattaatgg tctggaaatg gctttcctca gcgctgagga aaaacgcgca
     1081 ctgcgagaaa aagtcgccgc gaagtaacaa aatggatggt gcaaatgcac accatttttc
     1141 atgcaaggca caaagtcgcg cgatgtttcg gcggattcga tgcccagctc aatcaactcc
//

GenBank-Updates@genbank.bio.net (03/20/91)

LOCUS       ECOADD       1200 bp ds-DNA             BCT       20-MAR-1991
DEFINITION  E.coli adenosine deaminase (add) gene, complete cds.
ACCESSION   M59033 J05320
KEYWORDS    adenosine deaminase.
SOURCE      E.coli K-12 DNA.
  ORGANISM  Escherichia coli
            Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
            Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Chang,Z., Nygaard,P., Chinault,A.C. and Kellems,R.E.
  TITLE     Deduced amino acid sequence of Escherichia coli adenosine deaminase
            reveals evolutionarily conserved amino acid residues: Implications
            for catalytic function
  JOURNAL   Biochemistry 30, 2273-2280 (1991)
  STANDARD  simple staff_entry
FEATURES             Location/Qualifiers
     -35_signal      49..54
                     /note="consensus"
                     /gene="add"
     -10_signal      67..72
                     /note="consensus"
                     /gene="add"
     misc_feature    67..88
                     /note="operator"
                     /gene="add"
     mRNA            78..1116
                     /product="adenosine deaminase"
                     /gene="add"
     mRNA            80..1116
                     /product="adenosine deaminase"
                     /gene="add"
     RBS             99..103
                     /gene="add"
     CDS             109..1107
                     /EC_number="3.5.4.4"
                     /product="adenosine deaminase"
                     /gene="add"
                     /codon_start=109
     terminator      1116..1139
                     /gene="add"
BASE COUNT      282 a    321 c    305 g    292 t
ORIGIN
        1 cgtcgttaac cccaattcgc aagtaaaaaa tcgttgcaat cgtggatttt taccctgctt
       61 tgtttttata atggtgcgca cttttatatc cagaaaaaga gtgcgaccat gattgatacc
      121 accctgccat taactgatat ccatcgccac cttgatggca acattcgtcc ccagaccatt
      181 cttgaacttg gccgccagta taatatctcg cttcctgcac aatccctgga aacactgatt
      241 ccccacgttc aggtcattgc caacgaaccc gatctggtga gctttctgac caaacttgac
      301 tggggcgtta aagttctcgc ctctcttgat gcctgtcgcc gcgtggcatt tgaaaacatt
      361 gaagatgcag cccgtcacgg cctgcactat gtcgagctgc gtttttcacc aggctacatg
      421 gcaatggcac atcagctgcc tgtagcgggt gttgtcgaag cggtgatcga tggcgtacgt
      481 gaaggttgcc gcacctttgg tgtgcaggcg aagcttatcg gcattatgag ccggaccttc
      541 gaagccgcct gtcagcaaga gctggaggcc tttttagccc accgtgacca gattaccgca
      601 cttgatttag ccggtgatga acttggtttc ccgggaagtc tgttcctttc tcacttcaac
      661 cgcgcgcgtg atgcgggctg gcatattacc gtccatgcag gcgaagctgc cgggccggaa
      721 agcatctggc aggcgattcg tgaactgggt gcggagcgta ttggacatgg cgtaaaagcc
      781 attgaagatc gggcgctgat ggattttctc gccgagcaac aaattggtat tgaatcctgt
      841 ctgacctcca atattcagac cagcaccgta gcagagctgg ctgcacatcc gctgaaaacg
      901 ttccttgagc atggcattcg tgccagcatt aacactgacg atcccggcgt acagggagtg
      961 gatatcattc acgaatatac cgttgccgcg ccagctgctg ggttatcccg cgagcaaatc
     1021 cgccaggcac agattaatgg tctggaaatg gctttcctca gcgctgagga aaaacgcgca
     1081 ctgcgagaaa aagtcgccgc gaagtaacaa aatggatggt gcaaatgcac accatttttc
     1141 atgcaaggca caaagtcgcg cgatgtttcg gcggattcga tgcccagctc aatcaactcc
//