GenBank-Updates@genbank.bio.net (03/27/91)
LOCUS HUMADH2E1 375 bp ds-DNA PRI 27-MAR-1991
DEFINITION Human class I alcohol dehydrogenase (ADH2) beta-1 subunit, allele 1
gene, exon 1.
ACCESSION M24308 K01883 M11831 M59843
KEYWORDS alcohol dehydrogenase; dehydrogenase.
SEGMENT 1 of 9
SOURCE Human leukocyte DNA (library of Lawn et al.), clone lambda-ADH15.
ORGANISM Homo sapiens
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Primates; Haplorhini; Catarrhini; Hominidae.
REFERENCE 1 (bases 1 to 375)
AUTHORS Duester,G., Smith,M., Bilanchone,V. and Hatfield,G.W.
TITLE Molecular analysis of the human class I alcohol dehydrogenase gene
family and nucleotide sequence of the gene encoding the beta
subunit
JOURNAL J. Biol. Chem. 261, 2027-2033 (1986)
STANDARD simple staff_entry
REFERENCE 2 (sites)
AUTHORS Winter,L.A., Stewart,M.J., Shean,M.L., Dong,Y., Poellinger,L.,
Okret,S., Gustafsson,J.-A. and Duester,G.
TITLE A hormone response element upstream from the human alcohol
dehydrogenase gene ADH2 consists of three tandem glucocorticoid
receptor binding sites
JOURNAL Gene 91, 233-240 (1990)
STANDARD full staff_review
COMMENT ADH is encoded by at least five genes that fall into three classes.
Class I, which evolved as a multigene family, contains a large
group of isoenzymes possessing various combinations of alpha, beta,
and gamma subunits, coded for by three genes designated ADH1, ADH2,
and ADH3, respectively. Class II contains the pi isoenzyme and
class III contains the chi isoenzyme [J. Biol. Chem. 261, 2027-2033
(1986)]. The majority of
Orientals have the 'atypical' 2-ADH-2 gene, producing an atypical
beta-2 subunit instead of the beta-1 subunit, which is predominant
in Caucasians [2].
The other human class I ADH1 beta subunit sequences are found under
accession X03350 D00137 M24309-M24317 M21692
FEATURES Location/Qualifiers
prim_transcript 273..>375
/note="ADH2 mRNA"
exon 343..360
/note="alcohol dehydrogenase beta-1 subunit precursor,
exon 1 (EC 1.1.1.1) /nomgen='ADH2' /map='4q21-q25'
/hgml_locus_uid='LR0050W'"
mat_peptide 346..360
/note="alcohol dehydrogenase beta-1 subunit"
intron 361..>375
/note="ADH2 intron A"
BASE COUNT 137 a 55 c 87 g 96 t
ORIGIN 1 bp upstream of EcoRI site; chromosome 4q21-q25.
1 gaattccaga ggccgggggg gggtgggaag tgaggaaaag agaaagtgat tacaatttat
61 cactttaact taatatttaa actaatgaaa acaaaatctt atctagaatt tggaagtcaa
121 tattttgatt gctggttcag taccctttta tctgttttga cagtctggga ataatccagt
181 gggtgtggct taaagacata gatcacgtgt ggaattggaa ttggatgtta cacaagcaaa
241 caaaataaat atctgtgcaa tatatctgct ttatgcactc aagcagagaa gaaatccaca
301 aagactcaca gtctgctggt gggcagagaa gacagaaacg acatgagcac agcaggaaaa
361 gtaagcaaaa aatat
//