GenBank-Updates@genbank.bio.net (03/27/91)
LOCUS SYNINFBH 555 bp ds-DNA SYN 27-MAR-1991
DEFINITION E.coli atpE gene ribosome binding site fused to Human
beta-interferon gene, complete cds.
ACCESSION M60648
KEYWORDS artificial gene; interferon-beta.
SOURCE Artificial DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 555)
AUTHORS Gross,G., Mielke,C., Hollatz,I., Blocker,H. and Frank,R.
TITLE RNA primary sequence or secondary structure in the translational
initiation region controls expression of two variant
interferon-beta genes in escherichia coli
JOURNAL J. Biol. Chem. 265, 17627-17636 (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 50..550
/product="interferon-beta"
/codon_start=50
BASE COUNT 178 a 116 c 124 g 137 t
ORIGIN
1 taatttacca acactactac gttttaactg aaacaaactg gagactgtta tgagctacaa
61 cttgcttgga ttcctacaaa gaagcagcaa ttttcagtgt cagaagctcc tgtggcaatt
121 gaatgggagg cttgaatact gcctcaagga caggatgaac tttgacatcc ctgaggagat
181 taagcagctg cagcagttcc agaaggagga cgccgcattg accatctatg agatgctcca
241 gaacatcttt gctattttca gacaagattc atctagcact ggctggaatg agactattgt
301 tgagaacctc ctggctaatg tctatcatca gataaaccat ctgaagacag tcctggaaga
361 aaaactggag aaagaagatt tcaccagggg aaaactcatg agcagtctgc acctgaaaag
421 atattatggg aggattctgc attacctgaa ggccaaggag tacagtcact gtgcctggac
481 catagtcaga gtggaaatcc taaggaactt ttacttcatt aacagactta caggttacct
541 ccgaaactga agatc
//