GenBank-Updates@genbank.bio.net (03/27/91)
LOCUS SYNINFBA 555 bp ds-DNA SYN 27-MAR-1991
DEFINITION E.coli atpE gene ribosome binding site fused to artificial
beta-interferon gene, complete cds.
ACCESSION M60649
KEYWORDS artificial gene; interferon-beta.
SOURCE Artificial DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 555)
AUTHORS Gross,G., Mielke,C., Hollatz,I., Blocker,H. and Frank,R.
TITLE RNA primary sequence or secondary structure in the translational
initiation region controls expression of two variant
interferon-beta genes in escherichia coli
JOURNAL J. Biol. Chem. 265, 17627-17636 (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 50..550
/product="interferon-beta"
/codon_start=50
BASE COUNT 157 a 143 c 109 g 146 t
ORIGIN
1 taatttacca acactactac gttttaactg aaacaaactg gagactgcca tgtcttacaa
61 cctgctgggt ttcctgcagc gttcttctaa cttccagtgc cagaaactgc tgtggcagct
121 gaacggtcgt ctggaatact gcctgaaaga ccgtatgaac ttcgacatcc cggaagaaat
181 caaacagctg cagcagttcc agaaagaaga cgctgctctg actatctacg aaatgctgca
241 gaacatcttc gctatcttcc gtcaggactc ttcttctact ggttggaacg aaactatcgt
301 tgaaaacctg ctggctaacg tttaccacca gatcaaccac ctgaaaactg ttctggaaga
361 aaaactggaa aaagaagact tcactcgtgg taaactgatg tcttctctgc acctgaaacg
421 ttactacggt cgtatcctgc actacctgaa agctaaagaa tactctcact gcgcttggac
481 tatcgttcgt gttgaaatcc tgcgtaactt ctacttcatc aaccgtctga ctggttacct
541 gcgtaactga agatc
//