GenBank-Updates@genbank.bio.net (03/22/91)
LOCUS ECOECOA 1053 bp ds-DNA BCT 22-MAR-1991
DEFINITION E.coli ecotin gene, complete cds.
ACCESSION M60876 J05757
KEYWORDS ecotin; pancreatic serine protease inhibitor.
SOURCE E.coli (strain K-12) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1053)
AUTHORS McGrath,M.E., Hynes,W., Sakanari,J., Fletterick,R.J. and Craik,C.S.
TITLE The sequence and reactive site of ecotin: A general inhibitor of
pancreatic serine proteases from E.coli
JOURNAL J. Biol. Chem. (1991) In press
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 274..762
/product="ecotin"
/evidence=EXPERIMENTAL
/codon_start=274
sig_peptide 274..333
/codon_start=274
mat_peptide 334..759
/product="ecotin"
/evidence=EXPERIMENTAL
/codon_start=334
BASE COUNT 298 a 245 c 272 g 238 t
ORIGIN
1 cgtagaggat caaaagagta gcgggaagcg tggcaaaaac gggcttttgc tcacatttca
61 aattggttat aaatatattt atatagcgat tgattcacca gagatatttc tgctggtttg
121 ctctctcatt agaatttaac actaaaagag caggtaaaat tgtctgaatg ttctttaagt
181 tattcataaa gcaaattaat aaatctgatg aatatgttaa ccttcagcga catcatcggt
241 gaaaacctat aaatgaagaa ggaaagcaaa aaaatgaaga ccattctacc tgcagtattg
301 tttgccgctt tcgctaccac ttccgcctgg gcggcagaaa gcgtccagcc actggaaaaa
361 atcgcgcctt atccacaagc tgaaaaaggg atgaagcgtc aggtgattca gttaaccccg
421 caagaagatg aatctaccct gaaagtagaa ctgttaatcg gtcagacgct ggaagtcgat
481 tgcaatttgc atcgtctcgg cgggaagctg gaaaacaaaa cgctggaagg ctggggctat
541 gattattatg tctttgataa agtcagttcc ccggtttcaa cgatgatggc ctgcccggat
601 ggcaagaaag agaagaaatt tgtcaccgcg tatctgggcg atgctggaat gctgcgttac
661 aacagcaagc tgccgatcgt ggtgtatacg ccagacaatg tagatgtgaa gtaccgcgtc
721 tggaaggcgg aagagaaaat tgacaacgcg gtagttcgct aaactgccgt gaagtgcggc
781 accccgtagg tcagacaagg cggtcagcga tccgacatcc aacgcccgag ccggttgcct
841 gatgcgacgc tggccgtctt atcaggccta caccgctgtg aagtgcggca ccccgtaggt
901 cagacaaggc ggtcacgcgc atccgacatc caacgcccga gccggttgcc tgatgcgacg
961 ctggcctctt atcaggccta caccgctgtg aagtgctcca ccccgtaggt cggataaggc
1021 ggtagcgatc cgacatctaa cgcaagccgt tgc
//GenBank-Updates@genbank.bio.net (03/28/91)
LOCUS ECOECOA 1053 bp ds-DNA BCT 28-MAR-1991
DEFINITION E.coli ecotin gene, complete cds.
ACCESSION M60876 J05757
KEYWORDS ecotin; pancreatic serine protease inhibitor.
SOURCE E.coli (strain K-12) DNA.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1053)
AUTHORS McGrath,M.E., Hynes,W., Sakanari,J., Fletterick,R.J. and Craik,C.S.
TITLE The sequence and reactive site of ecotin: A general inhibitor of
pancreatic serine proteases from E.coli
JOURNAL J. Biol. Chem. (1991) In press
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 274..762
/product="ecotin"
/evidence=EXPERIMENTAL
/codon_start=274
sig_peptide 274..333
/codon_start=274
mat_peptide 334..759
/product="ecotin"
/evidence=EXPERIMENTAL
/codon_start=334
BASE COUNT 298 a 245 c 272 g 238 t
ORIGIN
1 cgtagaggat caaaagagta gcgggaagcg tggcaaaaac gggcttttgc tcacatttca
61 aattggttat aaatatattt atatagcgat tgattcacca gagatatttc tgctggtttg
121 ctctctcatt agaatttaac actaaaagag caggtaaaat tgtctgaatg ttctttaagt
181 tattcataaa gcaaattaat aaatctgatg aatatgttaa ccttcagcga catcatcggt
241 gaaaacctat aaatgaagaa ggaaagcaaa aaaatgaaga ccattctacc tgcagtattg
301 tttgccgctt tcgctaccac ttccgcctgg gcggcagaaa gcgtccagcc actggaaaaa
361 atcgcgcctt atccacaagc tgaaaaaggg atgaagcgtc aggtgattca gttaaccccg
421 caagaagatg aatctaccct gaaagtagaa ctgttaatcg gtcagacgct ggaagtcgat
481 tgcaatttgc atcgtctcgg cgggaagctg gaaaacaaaa cgctggaagg ctggggctat
541 gattattatg tctttgataa agtcagttcc ccggtttcaa cgatgatggc ctgcccggat
601 ggcaagaaag agaagaaatt tgtcaccgcg tatctgggcg atgctggaat gctgcgttac
661 aacagcaagc tgccgatcgt ggtgtatacg ccagacaatg tagatgtgaa gtaccgcgtc
721 tggaaggcgg aagagaaaat tgacaacgcg gtagttcgct aaactgccgt gaagtgcggc
781 accccgtagg tcagacaagg cggtcagcga tccgacatcc aacgcccgag ccggttgcct
841 gatgcgacgc tggccgtctt atcaggccta caccgctgtg aagtgcggca ccccgtaggt
901 cagacaaggc ggtcacgcgc atccgacatc caacgcccga gccggttgcc tgatgcgacg
961 ctggcctctt atcaggccta caccgctgtg aagtgctcca ccccgtaggt cggataaggc
1021 ggtagcgatc cgacatctaa cgcaagccgt tgc
//