[bionet.molbio.genbank.updates] E.coli mini-F plasmid repE mutant gene.

GenBank-Updates@genbank.bio.net (03/28/91)

LOCUS       FPLMREPE       72 bp ds-DNA             BCT       28-MAR-1991
DEFINITION  E.coli mini-F plasmid repE mutant gene.
ACCESSION   M63628
KEYWORDS    replication initiation protein.
SOURCE      Mini-F plasmid from E.coli DNA.
  ORGANISM  Plasmid F
            Prokaryota; Bacteria.
REFERENCE   1  (bases 1 to 72)
  AUTHORS   Kawasaki,Y., Wada,C. and Yura,T.
  TITLE     Mini-F plasmid mutants able to replicate in the Absence of sigma-32
            mutations in the repE coding region producing hyperactive initiator
            protein
  JOURNAL   J. Bacteriol. 173, 1064-1072 (1991)
  STANDARD  full staff_entry
FEATURES             Location/Qualifiers
     CDS             <1..72
                     /note="mutant gene with transposon insertion"
                     /product="replication initiator protein"
                     /gene="repE"
                     /codon_start=1
     transposon      20..>72
                     /note="Tn3"
BASE COUNT       19 a     15 c     20 g     18 t
ORIGIN
        1 cgcgatatca cttccatgag gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
       61 ttttggtcat ga
//