GenBank-Updates@genbank.bio.net (03/28/91)
LOCUS STRLACZZ 3234 bp ds-DNA BCT 28-MAR-1991 DEFINITION Streptococcus thermophilus beta-D-galactosidase (lacZ) gene, complete cds. ACCESSION M63636 M37204 KEYWORDS beta-D-galactosidase; lacZ gene. SOURCE Streptococcus thermophilus (strain A054; ATCC 19258) DNA, clone pCS200. ORGANISM Streptococcus thermophilus Prokaryota; Bacteria; Firmicutes; Gram-positive cocci; Streptococcaceae. REFERENCE 1 (bases 1 to 3234) AUTHORS Schroeder,C.J., Robert,C., Lenzen,G., McKay,L.L. and Mercenier,A. TITLE Analysis of the lacZ sequences from two Streptococcus thermophilus strains: Comparison with Escherichia coli and Lactobacillus bulgaricus beta-galactosidase sequences JOURNAL J. Gen. Microbiol. 137, 369-380 (1991) STANDARD full staff_entry FEATURES Location/Qualifiers RBS 139..145 /gene="lacZ" CDS 154..3234 /EC_number="3.2.1.23" /product="beta-D-galactosidase" /gene="lacZ" /codon_start=154 -35_signal 217..222 /gene="lacZ" -10_signal 241..246 /gene="lacZ" RBS 267..274 /gene="lacZ" CDS 277..3234 /EC_number="3.2.1.23" /product="beta-D-galactosidase" /gene="lacZ" /codon_start=277 BASE COUNT 1036 a 550 c 646 g 1002 t ORIGIN 1 tcaccaatta ttgagaaaaa tggtcttgat gacacagtac ttgtcactgt aactaattca 61 gaaaaattca gtgctttcca tcttgaacaa aaagttggag aaaaggtaga agctttgtct 121 gaagttatta ccttcaaaaa aggagaataa tctatgaaca tgactgaaaa aattcaaact 181 tatttaaacg atccaaagat tgttagcgtt aatactgttg atgctcactc agatcataag 241 tattttgaat ctcttgaaga attttctgaa ggggagatga agttaagaca atctcttaat 301 ggaaaatgga aaattcacta tgctcagaat acaaatcagg ttttaaaaga cttttataaa 361 acagaatttg atgaaactga tttgaatttc atcaatgtac caggtcattt agagcttcaa 421 ggttttggtt ctccacaata tgtgaatacc caatatcctt gggatggtaa agaattcctt 481 cgtccacctc aagttcctca agaatcaaat gctgttgcat catacgttaa acattttacc 541 ttgaatgatg cattaaaaga taaaaaagta tttatctcat tccaaggggt tgctacttcc 601 atctttgtat gggtcaatgg taacttcgta ggatacagtg aagattcatt tacacctagt 661 gaatttgaaa ttagtgatta ccttgttgaa ggtgataaca agttggcggt agctgtttat 721 cgttactcta cagcaagctg gttggaagac caagacttct ggagacttta cggtattttt 781 agagatgttt acttgtatgc tattccaaaa gttcacgttc aagatctctt tgttaaggga 841 gattatgatt accaaacaaa agcaggtcaa ttggatattg atttgaagac tgttggtgat 901 tatgaagaca agaagattaa atatgttctt tcagattatg aaggcatcgt tacagaaggt 961 gatgcatctg ttaatggtga cggtgaacta tctgtaagtc ttgaaaatct taaaatcaaa 1021 ccttggagtg ctgaaagtcc taaactttac gatttgatcc ttcatgtttt ggatgatgac 1081 caagttgttg aagtcgttcc agttaaagtt ggatttagac gctttgaaat taaagataaa 1141 cttatgcttt tgaatggtaa gagaattgtc tttaaagggg ttaacagaca cgaatttaac 1201 gctagaacag gacgttgtat cactgaagaa gatatgcttt gggatatcaa agtgatgaaa 1261 caacataaca tcaatgctgt tcgtacttca cactatccta accaaacacg ttggtatgaa 1321 ttgtgtgatg aatatggact ttatgttatc gatgaagcca accttgaaac acacggtaca 1381 tggcaaaaac ttggtctatg cgaaccttca tggaatatcc cagctagtga accagaatgg 1441 ttgcctgctt gtttggatcg tgccaataac atgttccaac gcgataagaa ccacgctagt 1501 gttatcattt ggtcttgtgg taatgaatca tatgctggta aagatattgc tgacatggct 1561 gattacttcc gtagtgttga caatactcgt ccagttcact atgaaggtgt tgcatggtgt 1621 cgtgagtttg attacattac agacatcgaa agtcgtatgt atgcgaaacc agctgatatc 1681 gaagaatacc tcacaactgg taaactagtt gatctttcaa gcgttagtga taaacacttt 1741 gcttcaggta acctaactaa caaacctcaa aaaccttata tttcatgtga atacatgcac 1801 acaatgggta actctggtgg tggattgcaa ctctacactg acttagagaa atatccagaa 1861 taccaaggtg gatttatttg ggacttcatt gaccaagcta tttacaaaac acttccaaat 1921 ggtagcgaat tcctatcata tggtggtgac tggcatgata gaccttctga ctacgaattt 1981 tgtggaaatg gtatcgtctt tgcagatcgt accctaactc caaaacttca aacagttaaa 2041 catctttact ctaatattaa gattgctgtt gatgaaaaat cagtaactat caagaatgat 2101 aatctcttcg aagatctttc tgcttatact ttcctagcta gagtttacga agatggtaga 2161 aaagttagtg aaagtgaata tcactttgat gttaaaccag gcgaagaagc aacattccca 2221 gttaactttg tagtcgaggc ttcaaattct gaacaaattt acgaagttgc ttgtgttctg 2281 agggaagcaa ctgaatgggc tcctaaaggt catgaaattg ttcgtggtca atatgttgtt 2341 gaaaagatta gcactgaaac accagttaaa gcacctttga atgttgttga aggcgacttc 2401 aacatcggta ttcaaggaca aaacttctca atcttgcttt cacgtgcaca aaatacttta 2461 gtatctgcta agtataatgg tgttgaattc attgagaaag gtcctaaact tagcttcact 2521 cgtgcttaca ctgacaacga tcgtggtgct ggatatccat tcgaaatggc aggctggaag 2581 gttgctggaa attatagtaa agttacagat actcaaattc aaatcgaaga cgactctgtt 2641 aaagtgactt atgttcatga attgccaggc ttgtctgatg tcgaagttaa ggtaacttat 2701 caagttgatt acaagggtcg aatctttgtt actgcaaact atgatggtaa agcaggtttg 2761 ccaaacttcc ctgaatttgg tctagaattt gctatcggtt cacaatttac aaaccttagc 2821 tattatggat acggtgcaga agaaagctac cgtgataaac ttcctggtgc ctatcttggt 2881 cgatatgaaa catctgttga aaagacattt gctccatatc taatgccaca agaatctggt 2941 aatcactatg gtactcgtga attcacagta tctgatgata accataatgg tcttaaattc 3001 accgcactta ataaagcatt cgaattcagt gctttgcgta acagtactga acaaattgaa 3061 aatgctcgtc accaatatga gttgcaagaa tctgatgcta catggattaa agttcttgct 3121 gctcaaatgg gtgtaggtgg tgacgacaca tggggtgctc cagttcatga cgaattcttg 3181 cttagctcag cagatagcta tcaattaagc ttcatgattg aaccactaaa ttag //