GenBank-Updates@genbank.bio.net (03/29/91)
LOCUS LACNISC 1222 bp ds-DNA BCT 29-MAR-1991
DEFINITION L.lactis insertion sequence IS981.
ACCESSION M33933
KEYWORDS insertion sequence.
SOURCE L.lactis (strain LM0230) DNA, clone pKM40.
ORGANISM Lactococcus lactis
Prokaryota; Bacteria; Firmicutes; Regular asporogenous rods;
Lactobacillaceae.
REFERENCE 1 (bases 1 to 1222)
AUTHORS Polzin,K.M. and McKay,L.L.
TITLE Identification, DNA sequence, and distribution of IS981, a new,
high-copy-number insertion sequence in lactococci
JOURNAL Appl. Environ. Microbiol. 57, 734-743 (1991)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by K.M.Polzin 27-APR-1990.
University of Minnesota
Dept. of Food Science
1334 Eckles Avenue, St. Paul, MN 55108
e-mail: kayla@molbio.cbs.umn.edu
FEATURES Location/Qualifiers
repeat_region 1..40
/note="5' inverted repeat"
misc_signal 33..38
/note="-10 region"
misc_binding 44..50
/note="ribosomal binding site (put.)"
CDS 58..318
/note="ORF1"
/codon_start=58
misc_signal 269..274
/note="-35 region"
misc_signal 294..299
/note="-10 region"
CDS 330..1169
/note="ORF2"
/codon_start=330
repeat_region 1183..1222
/note="3' inverted repeat"
BASE COUNT 442 a 233 c 206 g 341 t
ORIGIN 132 bp upstream of PvuII site.
1 gatgttatcc ttaaatctta gagtcactat tgtataattt agacaaagga caaaaacatg
61 caaaaacgct actcaaaaga atttaaagaa acccttatcg ccttctatca ttctggtcaa
121 tccgtcaccc agctgtctaa agaatatgac gtggcccctg caacaattta taaatggata
181 gacctctact ctaaatctaa tgaaagctcc gtctctaaag ctgattttct agaattaaaa
241 agacaactgg ctaaagttaa ggaagaacga gacatcttaa aaaaagtatt gaccatattc
301 gccgagaaaa agaagtgagt gctgcggata tggctcaaac catacaaact ttagcactca
361 atgtcagact aagctgtcaa ctccttgatg ttcctgaatc aagttattat gaacggatta
421 accgacaccc atctaaaact caattaagga gacaatacct gtcactcaaa atttctcaac
481 tcttcaatgc taaccgagga atctatggtg ctcctaaaat tcatcatctt ctacttaaac
541 aaggggaaaa agtcgggtta aaactggtac agaagctaat gaagcaactt caactcaagt
601 ctgtagtcat taagaaattt aagcctggat actcactaag tgatcacatc aatcgaaaaa
661 atctcataca gactgaacct acaaagaaaa ataaggtttg gtcaaccgac attacttata
721 ttcctactca acaaggatgg gcttatctct caaccattat ggatcgttat actaaaaaag
781 tcattgcttg ggatttgggc aagcgaatga ctgtagaatt agtgcaaaga actttaaata
841 aggccattaa atcacaagac tatccagaag ctgttattct tcattctgac caaggaagcc
901 agtatacgag tctagagtat gaagagttgc ttaagtatta tgggatgact cactctttca
961 gtcgaagggg atacccttat cataatgcca gtcttgaatc ttggcatgga catttaaaaa
1021 gagagtgggt gtaccaattt aaatataaga actttgaaga agcctatcag agtattttct
1081 ggtacatcga agccttttat aattcaaaac gaatccatca aagtttaggg tatcttacac
1141 ctaatcaatt tgaaaaggta agtgcttaaa ataaatagat taaaattcta cgtttgttac
1201 tctaaaaact tgacttaacg tc
//