GenBank-Updates@genbank.bio.net (10/31/90)
LOCUS PSEXYLMA 2353 bp ds-DNA circular BCT 31-OCT-1990
DEFINITION TOL plasmid of P.putida xylene monooxygenase (xylM and xylA) genes,
complete cds.
ACCESSION M37480
KEYWORDS xylene monooxygenase.
SOURCE TOL plasmid of P.putida DNA.
ORGANISM TOL plasmid
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 2353)
AUTHORS Suzuki,M., Hayakawa,T., Shaw,J.P., Rekik,M. and Harayama,S.
TITLE Primary structure of xylene monooxygenase: Similarities to and
differences from the alkane-hydroxylation system
JOURNAL Unpublished (1990)
STANDARD simple staff_entry
COMMENT Draft entry and computer-readable sequence for [1] kindly submitted
by Harayama,S., 03-AUG-1990. Dept of Med Biochem, CMU University
of Geneva 1 rue Michel-Servet 1211 Geneva 4, Switzerland
FEATURES from to/span description
pept 28 1137 xylene monooxygenase (xyl M)
pept 1287 2339 xylene monooxygenase (xylA)
signal 18 21 xylM ribosome binding site
signal 1274 1276 xylA ribosome binding site
BASE COUNT 546 a 518 c 630 g 659 t
ORIGIN
1 gtcgacaata ataaagtagg tggatatatg gacacgcttc gttattacct gattcctgtt
61 gttactgctt gcgggctgat cggattttac tatggtggct attgggtttg gcttggggcg
121 gcaacattcc ctgcactgat ggtgcttgat gtcattttac cgaaggattt ttcggccaga
181 aaggtaagtc cctttttcgc agaccttacc cagtatttgc agttaccatt aatgatcggt
241 ctatatgggc tccttgtctt cggagttgaa aacgggcgta tcgaacttag tgagccgtta
301 caagtggcag ggtgcattct ttctttggct tggcttagtg gtgtgccaac tcttccggtt
361 tcgcatgagt tgatgcatcg tcgccactgg ttgcctcgga aaatggcgca gctattggct
421 atgttttatg gtgatccgaa ccgagacatt gcccatgtca acacgcatca cctttactta
481 gatacgcctc tcgatagcga tactccgtac cgtggtcaga caatttacag tttcgtgatc
541 agtgcgacag ttggttccgt caaagatgcg ataaagattg aggctgaaac tttacgtaga
601 aaaggacagt caccgtggaa tttgtccaac aaaacatatc aatatgtcgc acttctgctc
661 gctctgcctg gcttggtttc ttatctgggc gggccagcat tagggttggt tacgattgct
721 tcgatgatta ttgcgaaagg gatagtcgag ggttttaatt actttcagca ctatggttta
781 gtacgcgatt tagatcagcc tatcctcctg caccacgcgt ggaatcatat gggaacaatt
841 gtgcgcccgc tgggttgcga aattactaac catatcaatc atcatattga cggctataca
901 cggttctatg agttgcgtcc ggaaaaagaa gccccgcaga tgccttcgct ctttgtgtgt
961 ttccttctag ggcttattcc gcctctttgg ttcgctctca ttgcaaaacc aaagttgaga
1021 gactgggacc agcggtacgc aactccaggt gagcgcgaac tggctatggc tgcaaataaa
1081 aaagcgggat ggccactgtg gtgtgaaagt gaactgggtc gggtggctag catttgataa
1141 tacaggcagt ctaaaccgtt tcgactgaga ccgtagataa gcgaagagcg aacgaactct
1201 acggtctctt tttgaaaaaa acgttccaaa tgggacaaag tttccgtttt ttctaattaa
1261 cgggcgtcta actgagcgat gggtttatga atgagttttt taagaaaatc tctggtttat
1321 ttgtgccgcc tccggaatct accgtttcag tcagagggca ggggtttcag tttaaggtgc
1381 cacgcgggca aaccattctg gaaagcgctc tgcatcaagg aattgccttt ccgcatgatt
1441 gcaaagtcgg atcttgtggg acatgtaaat ataaactgat atctggcagg gtcaatgagt
1501 tgacctcttc tgctatgggt ctgagtggcg atctgtatca gtccggctat cgtttgggtt
1561 gtcaatgcat accaaaagaa gatctcgaga tagagctaga cacagtgctc gggcaggcgt
1621 tagttccaat agaaacgagt gccttgatta gtaagcagaa acggctggcg cacgatatag
1681 tcgagatgga agtagtgccc gataagcaga tagccttcta ccccggccag tatgcagatg
1741 tagaatgtgc agaatgctct gctgtaagga gttattcttt ttccgctccg ccccaacctg
1801 acggctccct gagcttccat gttcgccttg tcccaggtgg agttttcagt ggttggctat
1861 ttggtggcga tcgtacagga gcgacactaa ccctgcgagc gccttatgga cagttcgggc
1921 tccatgagag caatgccacg atggtctgcg tagccggcgg aacggggctt gctccaatta
1981 aatgtgtttt gcagagcatg acccaggccc agcgagagcg tgatgtgttg ttgttctttg
2041 gagctcgtca acaacgtgac ctatattgcc tcgacgaaat agaagcgctg caactcgatt
2101 ggggtgggcg cttcgagctt attccagttt tgtccgaaga gtcttctacg tcgtcatgga
2161 aagggaaacg tggcatggta accgagtatt ttaaggagta cctcactggg cagccttatg
2221 aaggatacct ttgcgggccg ccccctatgg tggacgctgc cgagaccgag ctcgttcgac
2281 ttggtgttgc gcgggaatta gtgtttgcgg accgttttta taatagacct ccttgctagc
2341 agtagcagaa gct
//GenBank-Updates@genbank.bio.net (03/29/91)
LOCUS PSEXYLMA 2353 bp ds-DNA BCT 29-MAR-1991
DEFINITION TOL plasmid of P.putida xylene monooxygenase (xylM and xylA) genes,
complete cds.
ACCESSION M37480
KEYWORDS xylene monooxygenase.
SOURCE TOL plasmid of P.putida DNA.
ORGANISM TOL plasmid
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 2353)
AUTHORS Suzuki,M., Hayakawa,T., Shaw,J.P., Rekik,M. and Harayama,S.
TITLE Primary Structure of xylene monooxygenase: similarities to and
differences from the alkane hydroxylation system
JOURNAL J. Bacteriol. 173, 1690-1695 (1991)
STANDARD simple staff_entry
COMMENT Draft entry and computer-readable sequence for [Unpublished (1990)]
kindly submitted
by Harayama,S., 03-AUG-1990.
Dept of Med Biochem, CMU
University of Geneva
1 rue Michel-Servet
1211 Geneva 4, Switzerland
FEATURES Location/Qualifiers
RBS 18..21
/gene="xylM"
CDS 28..1137
/product="xylene monooxygenase"
/gene="xylM"
/codon_start=28
RBS 1274..1276
/product="xylene monooxygenase"
/gene="xylA"
CDS 1287..2339
/product="xylene monooxygenase"
/gene="xylA"
/codon_start=1287
BASE COUNT 546 a 518 c 630 g 659 t
ORIGIN
1 gtcgacaata ataaagtagg tggatatatg gacacgcttc gttattacct gattcctgtt
61 gttactgctt gcgggctgat cggattttac tatggtggct attgggtttg gcttggggcg
121 gcaacattcc ctgcactgat ggtgcttgat gtcattttac cgaaggattt ttcggccaga
181 aaggtaagtc cctttttcgc agaccttacc cagtatttgc agttaccatt aatgatcggt
241 ctatatgggc tccttgtctt cggagttgaa aacgggcgta tcgaacttag tgagccgtta
301 caagtggcag ggtgcattct ttctttggct tggcttagtg gtgtgccaac tcttccggtt
361 tcgcatgagt tgatgcatcg tcgccactgg ttgcctcgga aaatggcgca gctattggct
421 atgttttatg gtgatccgaa ccgagacatt gcccatgtca acacgcatca cctttactta
481 gatacgcctc tcgatagcga tactccgtac cgtggtcaga caatttacag tttcgtgatc
541 agtgcgacag ttggttccgt caaagatgcg ataaagattg aggctgaaac tttacgtaga
601 aaaggacagt caccgtggaa tttgtccaac aaaacatatc aatatgtcgc acttctgctc
661 gctctgcctg gcttggtttc ttatctgggc gggccagcat tagggttggt tacgattgct
721 tcgatgatta ttgcgaaagg gatagtcgag ggttttaatt actttcagca ctatggttta
781 gtacgcgatt tagatcagcc tatcctcctg caccacgcgt ggaatcatat gggaacaatt
841 gtgcgcccgc tgggttgcga aattactaac catatcaatc atcatattga cggctataca
901 cggttctatg agttgcgtcc ggaaaaagaa gccccgcaga tgccttcgct ctttgtgtgt
961 ttccttctag ggcttattcc gcctctttgg ttcgctctca ttgcaaaacc aaagttgaga
1021 gactgggacc agcggtacgc aactccaggt gagcgcgaac tggctatggc tgcaaataaa
1081 aaagcgggat ggccactgtg gtgtgaaagt gaactgggtc gggtggctag catttgataa
1141 tacaggcagt ctaaaccgtt tcgactgaga ccgtagataa gcgaagagcg aacgaactct
1201 acggtctctt tttgaaaaaa acgttccaaa tgggacaaag tttccgtttt ttctaattaa
1261 cgggcgtcta actgagcgat gggtttatga atgagttttt taagaaaatc tctggtttat
1321 ttgtgccgcc tccggaatct accgtttcag tcagagggca ggggtttcag tttaaggtgc
1381 cacgcgggca aaccattctg gaaagcgctc tgcatcaagg aattgccttt ccgcatgatt
1441 gcaaagtcgg atcttgtggg acatgtaaat ataaactgat atctggcagg gtcaatgagt
1501 tgacctcttc tgctatgggt ctgagtggcg atctgtatca gtccggctat cgtttgggtt
1561 gtcaatgcat accaaaagaa gatctcgaga tagagctaga cacagtgctc gggcaggcgt
1621 tagttccaat agaaacgagt gccttgatta gtaagcagaa acggctggcg cacgatatag
1681 tcgagatgga agtagtgccc gataagcaga tagccttcta ccccggccag tatgcagatg
1741 tagaatgtgc agaatgctct gctgtaagga gttattcttt ttccgctccg ccccaacctg
1801 acggctccct gagcttccat gttcgccttg tcccaggtgg agttttcagt ggttggctat
1861 ttggtggcga tcgtacagga gcgacactaa ccctgcgagc gccttatgga cagttcgggc
1921 tccatgagag caatgccacg atggtctgcg tagccggcgg aacggggctt gctccaatta
1981 aatgtgtttt gcagagcatg acccaggccc agcgagagcg tgatgtgttg ttgttctttg
2041 gagctcgtca acaacgtgac ctatattgcc tcgacgaaat agaagcgctg caactcgatt
2101 ggggtgggcg cttcgagctt attccagttt tgtccgaaga gtcttctacg tcgtcatgga
2161 aagggaaacg tggcatggta accgagtatt ttaaggagta cctcactggg cagccttatg
2221 aaggatacct ttgcgggccg ccccctatgg tggacgctgc cgagaccgag ctcgttcgac
2281 ttggtgttgc gcgggaatta gtgtttgcgg accgttttta taatagacct ccttgctagc
2341 agtagcagaa gct
//