GenBank-Updates@genbank.bio.net (02/06/91)
LOCUS BMEGPRA 1589 bp ds-DNA BCT 06-FEB-1991
DEFINITION Bacillus megaterium spore protease (gpr) gene, complete cds.
ACCESSION M55262
KEYWORDS spore protease.
SOURCE B.megaterium (strain QMB1551) DNA.
ORGANISM Bacillus megaterium
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 1589)
AUTHORS Sussman,M.D. and Setlow,P.
TITLE Cloning, nucleotide sequence and regulation of the Bacillus
subtilis gpr gene which codes for the protease that initiates
degradation of small, acid-soluble proteins during spore
germination
JOURNAL Unpublished (1990)
STANDARD full staff_entry
FEATURES Location/Qualifiers
-35_signal 45..50
/gene="gpr"
/partial
-10_signal 69..75
/gene="gpr"
/partial
mRNA 81..1223
/gene="gpr"
/partial
RBS 95..101
/gene="gpr"
/partial
CDS 108..1223
/function="initiates degradation of small, acid-soluble
proteins during spore germination"
/gene="gpr"
/codon_start=108
/product="spore protease"
BASE COUNT 514 a 262 c 375 g 438 t
ORIGIN
1 tgtggctgat attgcgccct tccttccact tttcatttct taaatgcatg aatctgccag
61 tcctcggtga caataatttc aacaaagatt gtctaggagg ttaagaaatg gaaaaagaac
121 ttgatttaag tcaatatagc gtgcgcacgg atttagctgt cgaagcaaaa gacatagcgt
181 tagaaaatca gcctaaaccg aataaccagt cagaaatcaa aggcgtaatt gtaaaagaaa
241 aagaagaaca aggtgtcaaa atctcaatgg tagaaattac ggaagagggc gcagaagcaa
301 tcggtaagaa aaaaggtcga tatgtaaccc ttgaatcggt aggcattcgc gagcaggata
361 ctgaaaagca agaagaggcc atggaagaag tatttgctaa agagctgaat ttttttatta
421 aaagcctcaa cattccagat gatgctagct gtcttgtagt gggtcttgga aacttaagtg
481 ttacaccaga tgcgcttgga ccaaaggcag tagataattt gttgattaca agacatttgt
541 ttgagctaca gcctgaaagc gtgcaagatg gctttagacc cgttagcgcc attgttccgg
601 gagtaatggg aatgacgggt attgagacaa gtgatattat ctttggcgtt gtaaagaaag
661 tgaatccaga ttttatcatt gcaatcgatg cgcttgccgc tcgttcgatt gaacgggtaa
721 atgctaccat tcaaatttca gattcaggta ttcatccggg gtctggggtt ggaaacaagc
781 gtaaagaaat cagttatgaa acacttggca ttccagttat tgcaataggt attccaacgg
841 tagtggatgc tgtttccatt acaagcgata caattgactt tatcttaaaa catttcggcc
901 gagaaatgaa agagcaggga aagccgtcta agtcgctgct tccgtccggt atgacgtttg
961 gagaaaaaaa gaagctcacg gaggatgact tacccaatga agagcagcgt caaacgtatc
1021 tcggtatgat tggcaccctt ccagatgaag aaaaaagacg tcttattcat gaagtgcttg
1081 cacctttagg ccataatttg atggttacgc caaaagaagt ggatatgttt attgaagata
1141 tggctaatgt agttgcagga ggacttaacg ctgctttgca tcatgaagtg gatcaagaga
1201 actttggggc atatactcat taatacgctt ttaggtgaat gagttcttca caatatagtc
1261 ataatggtta gtactacaga agataaagaa gtaaggggcc taataatatg attaaattta
1321 ctttaaaatc agtagtgcta accatttttt tactgtgcgt cgtattttta gggatgcaga
1381 cggctcagca agggttgatt aaaatgaagg gatataatga tccttcgatt gaacatgtta
1441 ttaatgtgtc taaaacatca gatggtcagg tggaagctgc tgttttaggt tcaacaaaag
1501 tcgtgaacat tgatgaaaaa cagcagtttc taggaaaaac gaaaggcttt taactttttc
1561 tcctcgctag gtcaaagtct agcggaagg
//GenBank-Updates@genbank.bio.net (03/29/91)
LOCUS BMEGPRA 1589 bp ds-DNA BCT 29-MAR-1991
DEFINITION Bacillus megaterium spore protease (gpr) gene, complete cds.
ACCESSION M55262
KEYWORDS spore protease.
SOURCE B.megaterium (strain QMB1551) DNA.
ORGANISM Bacillus megaterium
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 1589)
AUTHORS Sussman,M.D. and Setlow,P.
TITLE Cloning, nucleotide sequence, and regulation of the bacillus
subtilis gpr gene, which codes for the protease that initiates
degradation of small, acid-soluble proteins during spore
germination
JOURNAL J. Bacteriol. 173, 291-300 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
-35_signal 45..50
/gene="gpr"
/partial
-10_signal 69..75
/gene="gpr"
/partial
mRNA 81..1223
/gene="gpr"
/partial
RBS 95..101
/gene="gpr"
/partial
CDS 108..1223
/function="initiates degradation of small, acid-soluble
proteins during spore germination"
/product="spore protease"
/gene="gpr"
/codon_start=108
BASE COUNT 514 a 262 c 375 g 438 t
ORIGIN
1 tgtggctgat attgcgccct tccttccact tttcatttct taaatgcatg aatctgccag
61 tcctcggtga caataatttc aacaaagatt gtctaggagg ttaagaaatg gaaaaagaac
121 ttgatttaag tcaatatagc gtgcgcacgg atttagctgt cgaagcaaaa gacatagcgt
181 tagaaaatca gcctaaaccg aataaccagt cagaaatcaa aggcgtaatt gtaaaagaaa
241 aagaagaaca aggtgtcaaa atctcaatgg tagaaattac ggaagagggc gcagaagcaa
301 tcggtaagaa aaaaggtcga tatgtaaccc ttgaatcggt aggcattcgc gagcaggata
361 ctgaaaagca agaagaggcc atggaagaag tatttgctaa agagctgaat ttttttatta
421 aaagcctcaa cattccagat gatgctagct gtcttgtagt gggtcttgga aacttaagtg
481 ttacaccaga tgcgcttgga ccaaaggcag tagataattt gttgattaca agacatttgt
541 ttgagctaca gcctgaaagc gtgcaagatg gctttagacc cgttagcgcc attgttccgg
601 gagtaatggg aatgacgggt attgagacaa gtgatattat ctttggcgtt gtaaagaaag
661 tgaatccaga ttttatcatt gcaatcgatg cgcttgccgc tcgttcgatt gaacgggtaa
721 atgctaccat tcaaatttca gattcaggta ttcatccggg gtctggggtt ggaaacaagc
781 gtaaagaaat cagttatgaa acacttggca ttccagttat tgcaataggt attccaacgg
841 tagtggatgc tgtttccatt acaagcgata caattgactt tatcttaaaa catttcggcc
901 gagaaatgaa agagcaggga aagccgtcta agtcgctgct tccgtccggt atgacgtttg
961 gagaaaaaaa gaagctcacg gaggatgact tacccaatga agagcagcgt caaacgtatc
1021 tcggtatgat tggcaccctt ccagatgaag aaaaaagacg tcttattcat gaagtgcttg
1081 cacctttagg ccataatttg atggttacgc caaaagaagt ggatatgttt attgaagata
1141 tggctaatgt agttgcagga ggacttaacg ctgctttgca tcatgaagtg gatcaagaga
1201 actttggggc atatactcat taatacgctt ttaggtgaat gagttcttca caatatagtc
1261 ataatggtta gtactacaga agataaagaa gtaaggggcc taataatatg attaaattta
1321 ctttaaaatc agtagtgcta accatttttt tactgtgcgt cgtattttta gggatgcaga
1381 cggctcagca agggttgatt aaaatgaagg gatataatga tccttcgatt gaacatgtta
1441 ttaatgtgtc taaaacatca gatggtcagg tggaagctgc tgttttaggt tcaacaaaag
1501 tcgtgaacat tgatgaaaaa cagcagtttc taggaaaaac gaaaggcttt taactttttc
1561 tcctcgctag gtcaaagtct agcggaagg
//