GenBank-Updates@genbank.bio.net (02/06/91)
LOCUS BSUGPRA 1431 bp ds-DNA BCT 06-FEB-1991
DEFINITION Bacillus subtilis spore protease (gpr) gene, complete cds.
ACCESSION M55263
KEYWORDS spore protease.
SOURCE B.subtilis (strain 168) DNA.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 1431)
AUTHORS Sussman,M.D. and Setlow,P.
TITLE Cloning, nucleotide sequence and regulation of the Bacillus
subtilis gpr gene which codes for the protease that initiates
degradation of small, acid-soluble proteins during spore
germination
JOURNAL Unpublished (1990)
STANDARD full staff_entry
FEATURES Location/Qualifiers
-35_signal 188..193
/gene="gpr"
/partial
-10_signal 212..218
/gene="gpr"
/partial
mRNA 224..1353
/gene="gpr"
/partial
RBS 233..239
/gene="gpr"
/partial
CDS 247..1353
/function="initiates degradation of small, acid-soluble
proteins during spore germination"
/gene="gpr"
/codon_start=247
/product="spore protease"
BASE COUNT 443 a 267 c 355 g 366 t
ORIGIN
1 tgtttgtttt tgtacgttta atcgctgatt taatgtttgg catgtgtttc acctcctaag
61 atacaacctg tagcacagtg tcttaaggtt aaatcttctt cacaatagaa caaattgtat
121 tctatcaaac acacctttag attgcaatat aaatgtaaag tatttttcat tgaaggttct
181 ctttttagca tgatttattc agcaaatggc aacaatatag gtacttaatg tgaaggaggc
241 ccctgtatga aaaagagtga gctggatgtg aatcagtatt tgatacggac cgatttggca
301 gtggaaacga aggaagcaat ggccaatcag caggctgtgc cgacaaaaga aattaaaggc
361 tttattgaaa aagagcgtga tcacggcggc attaaaatcc gcacagttga tgttacaaag
421 gaaggcgcag agctttcggg aaaaaaagaa gggcgctatt taacgcttga agcacaggga
481 atcagggaga atgattcgga aatgcaggag aaggtctctg ctgtctttgc ggaggaattc
541 tcagcttttc ttgaaaacct taatatctca aaagatgcga gctgtttgat cgttgggctc
601 ggcaactgga acgtgacacc tgatgcgctt ggaccgatgg cagtagaaaa tctgttggta
661 acaaggcact tgtttaagct tcagccggaa aacgtacagg agggttacag gcctgtcagt
721 gcttttgcac cgggtgtaat ggggataaca ggaatcgaaa caagtgacat tattaaaggt
781 gtgattgagc aatcgaagcc tgattttgtc atcgccattg atgcgttggc ggcgcgggcg
841 gtggaaaggg tcaacacaac cattcaaata tcggatacag gcattcaccc gggctctgga
901 gtggggaata aacgaaaaga tttaagcaaa gacacgcttg gtgttccggt gattgctatc
961 ggtgttccga cagtagttga tgccgtgaca attgccagcg acacggtgga ctacatatta
1021 aaacattttg gcagagaaat gaaggataac aggccatcaa gatcgcttgt tccggcaggc
1081 atgacgtttg ggaaaaagaa agtgctcact gaagacgatc ttcccgatca aaagcagcgc
1141 caatcgttcc taggtattgt cggaaccctt caagaagatg aaaaacggca gctcattcac
1201 gaggtgcttt cgcctttagg gcacaactta atggttacac cgaaggaagt cgattcattt
1261 atcgacgata tggcaaatgt tttagcgaac ggattaaaca cagcgcttca tgaaaaagtg
1321 tcacaagaaa acaaaggctc ctacaatcac taaacagttc tacttcctct agcttgttca
1381 tagagtaatt actagacaag cgctggaggg attcaatgag aaataaacgc a
//GenBank-Updates@genbank.bio.net (03/29/91)
LOCUS BSUGPRA 1431 bp ds-DNA BCT 29-MAR-1991
DEFINITION Bacillus subtilis spore protease (gpr) gene, complete cds.
ACCESSION M55263
KEYWORDS spore protease.
SOURCE B.subtilis (strain 168) DNA.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 1431)
AUTHORS Sussman,M.D. and Setlow,P.
TITLE Cloning, nucleotide sequence, and regulation of the bacillus
subtilis gpr gene, which codes for the protease that initiates
degradation of small, acid-soluble proteins during spore
germination
JOURNAL J. Bacteriol. 173, 291-300 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
-35_signal 188..193
/gene="gpr"
/partial
-10_signal 212..218
/gene="gpr"
/partial
mRNA 224..1353
/gene="gpr"
/partial
RBS 233..239
/gene="gpr"
/partial
CDS 247..1353
/function="initiates degradation of small, acid-soluble
proteins during spore germination"
/product="spore protease"
/gene="gpr"
/codon_start=247
BASE COUNT 443 a 267 c 355 g 366 t
ORIGIN
1 tgtttgtttt tgtacgttta atcgctgatt taatgtttgg catgtgtttc acctcctaag
61 atacaacctg tagcacagtg tcttaaggtt aaatcttctt cacaatagaa caaattgtat
121 tctatcaaac acacctttag attgcaatat aaatgtaaag tatttttcat tgaaggttct
181 ctttttagca tgatttattc agcaaatggc aacaatatag gtacttaatg tgaaggaggc
241 ccctgtatga aaaagagtga gctggatgtg aatcagtatt tgatacggac cgatttggca
301 gtggaaacga aggaagcaat ggccaatcag caggctgtgc cgacaaaaga aattaaaggc
361 tttattgaaa aagagcgtga tcacggcggc attaaaatcc gcacagttga tgttacaaag
421 gaaggcgcag agctttcggg aaaaaaagaa gggcgctatt taacgcttga agcacaggga
481 atcagggaga atgattcgga aatgcaggag aaggtctctg ctgtctttgc ggaggaattc
541 tcagcttttc ttgaaaacct taatatctca aaagatgcga gctgtttgat cgttgggctc
601 ggcaactgga acgtgacacc tgatgcgctt ggaccgatgg cagtagaaaa tctgttggta
661 acaaggcact tgtttaagct tcagccggaa aacgtacagg agggttacag gcctgtcagt
721 gcttttgcac cgggtgtaat ggggataaca ggaatcgaaa caagtgacat tattaaaggt
781 gtgattgagc aatcgaagcc tgattttgtc atcgccattg atgcgttggc ggcgcgggcg
841 gtggaaaggg tcaacacaac cattcaaata tcggatacag gcattcaccc gggctctgga
901 gtggggaata aacgaaaaga tttaagcaaa gacacgcttg gtgttccggt gattgctatc
961 ggtgttccga cagtagttga tgccgtgaca attgccagcg acacggtgga ctacatatta
1021 aaacattttg gcagagaaat gaaggataac aggccatcaa gatcgcttgt tccggcaggc
1081 atgacgtttg ggaaaaagaa agtgctcact gaagacgatc ttcccgatca aaagcagcgc
1141 caatcgttcc taggtattgt cggaaccctt caagaagatg aaaaacggca gctcattcac
1201 gaggtgcttt cgcctttagg gcacaactta atggttacac cgaaggaagt cgattcattt
1261 atcgacgata tggcaaatgt tttagcgaac ggattaaaca cagcgcttca tgaaaaagtg
1321 tcacaagaaa acaaaggctc ctacaatcac taaacagttc tacttcctct agcttgttca
1381 tagagtaatt actagacaag cgctggaggg attcaatgag aaataaacgc a
//