GenBank-Updates@genbank.bio.net (03/29/91)
LOCUS LACNSRA 1454 bp ds-DNA BCT 29-MAR-1991
DEFINITION L.lactis nisin-resistance protein (nsr) gene, complete cds.
ACCESSION M37002
KEYWORDS nisin-resistance.
SOURCE L.lactis lactis biovar diacetylactis (strain DRC3) DNA.
ORGANISM Lactococcus lactis
Prokaryota; Bacteria; Firmicutes; Regular asporogenous rods;
Lactobacillaceae.
REFERENCE 1 (bases 1 to 1454)
AUTHORS Froseth,B.R. and Mckay,L.L.
TITLE Molecular chatacterization of the nisin resistance region of
lactococcus lactis subsp. lactis biovar diacetylactis DRC3
JOURNAL Appl. Environ. Microbiol. 57, 804-811 (1991)
STANDARD full staff_entry
COMMENT Draft entry and computer-readable [or printed] sequence for
[Unpublished (1990)]
kindly submitted by L.L. McKay 17-JUL-1990.
Authors Address: L.L.McKay
Department of Food Science and Nutrition
University of Minnesota
1334 Eckles Avenue
St. Paul, MN 55108
FEATURES Location/Qualifiers
misc_signal 289..294
/note="-35 region"
misc_signal 311..316
/note="-10 region"
misc_binding 340..344
/note="ribosome binding site"
CDS 354..1310
/note="nisin-resistance protein"
/codon_start=354
misc_signal 1314..1352
/note="rho-independent transcription termination signal"
BASE COUNT 538 a 219 c 239 g 458 t
ORIGIN
1 gaattcctag ttgattattt agccatgact tgatacccga tagaatatct taaagtctct
61 ggttccagtg atttagctga ttttaacagt aaataatacg ctaaaagtat catctctaat
121 ttcaattgaa aaccttgagg cgaacgactt ttacaacgct cagctcctag atttgtcaaa
181 aaatagaaaa ctagtcataa cttcccatta tgtctaattt ttaaactttg agttaatatg
241 agagcgcaaa attacttcct agatttatcg tttacaatca gtccaaagtt gcaggatttt
301 ccattcaaat tatgttttaa tagtattaaa ataactgatg gagcatattt aatatgaaaa
361 taggtaagcg cattttatta ggtctagtgg cagtatgtgc tttattttta ggaattatct
421 atctttgggg gtataaattc aacatatatt tagtaccacc ctcccctcag aagtatgttc
481 gagttgcctt aaaaaatatg gatgaacttg ggctatttac tgattcaaaa gaatgggtag
541 aaactaaaaa aaagacgata gaagaaacat caaatgctaa aaactatgca gaaacaatcc
601 cttttttaca aaaagcgatt aaagttgcag gaggaaagca ttcttttatt gaacatgaag
661 aagacatatc aaaaagaagc atgacaaaat atataaaacc aaaggcagaa atcgaaggca
721 acactttaat attaactatt cctgaattta ctggaaatga tagtcaagca tctgattacg
781 ctaatttttt agaatcttca ttgcataaaa acaattataa tggggtaatt gttgatttga
841 gggggaatag aggtggagac ttatctccta tggtattagg attatccccc ctattgcctg
901 atggaactct atttacttat gttgataaaa gtagtcattc taaacctgtt gaactacaaa
961 atggagaaat aaatagtggc gggtcatcaa caaaaataag tgataataaa aaaattaaaa
1021 aagctcctat tgctgtatta atagataata atacagggag ctccggcgaa ttaaccgctt
1081 tgtgctttga gggaatacct aatgttaaat ttttgggttc tgattcagca ggttatactt
1141 ctgctaatca aaccgtctat ttatatgatg gctcaacatt acaaataact tctgcttttg
1201 taaaagacag aacaaataat atttataaaa attttcctat tagtccggac attcaaacaa
1261 ataatgctaa aagttctgca atagaatgga taaaatctca aataaagtaa agcaaaaaga
1321 cttgccaaga aaaacatatg gtaagtcttt tttgctagtc aaaacttaac gttatttact
1381 atgatatacc gaaagcaagt accaatgcag gtaaaatgtt gtttaaaagt gtatagctat
1441 actactctat cgtc
//