GenBank-Updates@genbank.bio.net (02/01/91)
LOCUS CDCMANABD 2155 bp ds-DNA BCT 01-FEB-1991
DEFINITION C.saccharolyticum beta-D-mannanase (manA) gene, complete cds.
ACCESSION M36063
KEYWORDS beta-D-mannanase.
SOURCE C.saccharolyticum DNA, clone lambda-NZP2.
ORGANISM Caldocellum saccharolyticum
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 2155)
AUTHORS Leuthi,E., Bhana Jasmat,N., Grayling,R.A., Love,D.R. and
Bergquist,P.L.
TITLE Cloning, sequence analysis and expression in Escherichia coli of a
gene coding for a beta-mannanase from the extremely thermophilic
bacteria Caldocellum saccharolyticum
JOURNAL Unpublished (1990)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence kindly submitted
by E.Luethi, 03-JUL-1990.
Author address: E.Luethi
University of Auckland
Department of Cellular and Molecular Biology
Private Bag
Auckland
NEW ZEALAND
email:SYSTEM@CELVAX.AUKUNI.AC.NZ
FEATURES Location/Qualifiers
CDS <1..685
/note="ORF1 (AA at 2)"
misc_binding 818..830
/note="manA ribosome binding site"
CDS 841..1881
/note="(E.C. 3.2.1.25)"
/product="beta-D-mannanase"
sig_peptide 841..963
/note="beta-D-mannanase signal peptide"
mat_peptide 964..1878
/note="beta-D-mannanase"
BASE COUNT 721 a 334 c 590 g 510 t
ORIGIN
1 gggtagcaat acatggtttg gattccaggc atggtcgatg cagagggtag cggagtatta
61 ctatgtgaca ggagataaag atgcaggggc actgcttgag aagtgggtaa gctggataaa
121 gagtgtagtg aagttgaata gtgatggtac atttgcgata ccatcgacgc ttgattggag
181 tgggcagcca gacacatgga atgggacata tacaggtaat ccgaacttgc atgtgaaggt
241 agtagattat gggacggatt taggaataac ggcatcactt gcgaatgcac tactttatta
301 cagtgcaggg acgaagaagt atggggtatt tgatgaggaa gcgaagaatt tagcgaagga
361 attgctggac aggatgtgga agttatacag ggatgagaaa ggtttatcgg cgccagagaa
421 gagagcggac tacaagaggt tctttgagca agaggtatac attccggcag gatggacagg
481 gaagatgccg aatggagatg taataaagag cggagttaag tttatagaca taaggagcaa
541 gtacaaacaa gatcctgatt ggccgaagtt agaggcggca tacaagtcag ggcaggtacc
601 ggagttcaga tatcacaggt tctgggcaca gtgtgacata gcaattgtta atgcaacata
661 tgaaattctg ttcggtaatc aataatgagt aggtaaatgg aaatttagcg gggtggcaca
721 tctataagtt tggtgtgctg cctcgctaaa atcctgtatg gaagtgttcg aaaaatagta
781 caaaaaaatg gcgaggtaaa atggcaaaaa tgcataaaaa agagggggtt aggttttaag
841 atgagactaa aaacaaaaat aagaaagaaa tggttgagtg tactatgtac agttgttttt
901 ttattgaaca ttttgtttat agcaaatgta acgattttac ccaaagttgg tgcggctaca
961 tctaatgatg gagtagtgaa gatagatacg agtacgttaa taggaaccaa tcacgcacat
1021 tgctggtaca gagatagact tgatacggca ttgcgtggaa ttaggtcatg gggtatgaac
1081 tctgtgaggg tagtgctgag taatggttac cgatggacga agataccagc aagtgaagtg
1141 gcaaatatta tatcattgtc aagaagtctt ggtttcaaag ctattatatt agaagtgcac
1201 gacacaacag gatatggaga agatggggca gcatgttcat tggcacaagc agtggaatat
1261 tggaaggaga taaagagcgt attagacggt aacgaagatt ttgtaattat aaacattggt
1321 aatgagccgt atgggaacaa taactatcaa aactgggtta atgacacgaa gaacgctata
1381 aaagcactta gagatgcagg attcaagcac acgataatgg tggatgcgcc gaactggggt
1441 caggattggt ctaatactat gagagataat gcccagagca taatggaagc agatccgctg
1501 cgcaatttgg tattttcgat tcatatgtat ggcgtataca atacagcaag caaggtcgaa
1561 gagtacatca aatcatttgt tgataagggg ttaccattgg ttattggaga atttggacat
1621 cagcacacag atggtgaccc tgatgaagaa gctattgtca ggtatgcaaa acagtacaag
1681 ataggattat ttagttggtc gtggtgtgga aattcgagct atgttgggta tttggacatg
1741 gtaaacaact gggaccccaa taatccaact ccatggggac aatggtataa aactaatgca
1801 attggtacat cttctacacc aacacctacg tcgacagtaa cgccaacgcc acccccacgc
1861 caacaccaac accgacagta acagcgacac caacaccaac acctacacct gttagcacac
1921 ctgcgacaag tgggcagata aaggtactgt atgctaacaa ggagacaaac agcactacaa
1981 acacgataag gccgtggttg aaggtagtga atagtggtag cagtagcata gatttgagca
2041 gggtaacgat aaggtactgg tacacggtag atggtgagag ggcacagact ggatatcaga
2101 ctgggcacag ataggagcaa gcaatgtcaa cattcaagtt tgtgaagctg agagt
//GenBank-Updates@genbank.bio.net (02/05/91)
LOCUS CDCMANABD 2155 bp ds-DNA BCT 05-FEB-1991
DEFINITION C.saccharolyticum beta-D-mannanase (manA) gene, complete cds.
ACCESSION M36063
KEYWORDS 1,4-beta-D-mannan mannanohydrolase; beta-D-mannanase.
SOURCE C.saccharolyticum DNA, clone lambda-NZP2.
ORGANISM Caldocellum saccharolyticum
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 2155)
AUTHORS Luthi,E., Bhana Jasmat,N., Grayling,R.A., Love,D.R. and
Bergquist,P.L.
TITLE Cloning, sequence analysis and expression in Escherichia coli of a
gene coding for a beta-mannanase from the extremely thermophilic
bacteria Caldocellum saccharolyticum
JOURNAL Appl. Environ. Microbiol. (1991) In press
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence kindly submitted
by E.Luthi, 03-JUL-1990.
Author address: E.Luthi
University of Auckland
Department of Cellular and Molecular Biology
Private Bag
Auckland
NEW ZEALAND
email:SYSTEM@CELVAX.AUKUNI.AC.NZ
FEATURES Location/Qualifiers
CDS <1..685
/note="ORF1 (AA at 2)"
misc_binding 818..830
/note="manA ribosome binding site"
CDS 841..1881
/note="(E.C. 3.2.1.78)"
/product="beta-D-mannanase"
sig_peptide 841..963
/note="beta-D-mannanase signal peptide"
mat_peptide 964..1878
/note="beta-D-mannanase"
BASE COUNT 721 a 334 c 590 g 510 t
ORIGIN
1 gggtagcaat acatggtttg gattccaggc atggtcgatg cagagggtag cggagtatta
61 ctatgtgaca ggagataaag atgcaggggc actgcttgag aagtgggtaa gctggataaa
121 gagtgtagtg aagttgaata gtgatggtac atttgcgata ccatcgacgc ttgattggag
181 tgggcagcca gacacatgga atgggacata tacaggtaat ccgaacttgc atgtgaaggt
241 agtagattat gggacggatt taggaataac ggcatcactt gcgaatgcac tactttatta
301 cagtgcaggg acgaagaagt atggggtatt tgatgaggaa gcgaagaatt tagcgaagga
361 attgctggac aggatgtgga agttatacag ggatgagaaa ggtttatcgg cgccagagaa
421 gagagcggac tacaagaggt tctttgagca agaggtatac attccggcag gatggacagg
481 gaagatgccg aatggagatg taataaagag cggagttaag tttatagaca taaggagcaa
541 gtacaaacaa gatcctgatt ggccgaagtt agaggcggca tacaagtcag ggcaggtacc
601 ggagttcaga tatcacaggt tctgggcaca gtgtgacata gcaattgtta atgcaacata
661 tgaaattctg ttcggtaatc aataatgagt aggtaaatgg aaatttagcg gggtggcaca
721 tctataagtt tggtgtgctg cctcgctaaa atcctgtatg gaagtgttcg aaaaatagta
781 caaaaaaatg gcgaggtaaa atggcaaaaa tgcataaaaa agagggggtt aggttttaag
841 atgagactaa aaacaaaaat aagaaagaaa tggttgagtg tactatgtac agttgttttt
901 ttattgaaca ttttgtttat agcaaatgta acgattttac ccaaagttgg tgcggctaca
961 tctaatgatg gagtagtgaa gatagatacg agtacgttaa taggaaccaa tcacgcacat
1021 tgctggtaca gagatagact tgatacggca ttgcgtggaa ttaggtcatg gggtatgaac
1081 tctgtgaggg tagtgctgag taatggttac cgatggacga agataccagc aagtgaagtg
1141 gcaaatatta tatcattgtc aagaagtctt ggtttcaaag ctattatatt agaagtgcac
1201 gacacaacag gatatggaga agatggggca gcatgttcat tggcacaagc agtggaatat
1261 tggaaggaga taaagagcgt attagacggt aacgaagatt ttgtaattat aaacattggt
1321 aatgagccgt atgggaacaa taactatcaa aactgggtta atgacacgaa gaacgctata
1381 aaagcactta gagatgcagg attcaagcac acgataatgg tggatgcgcc gaactggggt
1441 caggattggt ctaatactat gagagataat gcccagagca taatggaagc agatccgctg
1501 cgcaatttgg tattttcgat tcatatgtat ggcgtataca atacagcaag caaggtcgaa
1561 gagtacatca aatcatttgt tgataagggg ttaccattgg ttattggaga atttggacat
1621 cagcacacag atggtgaccc tgatgaagaa gctattgtca ggtatgcaaa acagtacaag
1681 ataggattat ttagttggtc gtggtgtgga aattcgagct atgttgggta tttggacatg
1741 gtaaacaact gggaccccaa taatccaact ccatggggac aatggtataa aactaatgca
1801 attggtacat cttctacacc aacacctacg tcgacagtaa cgccaacgcc acccccacgc
1861 caacaccaac accgacagta acagcgacac caacaccaac acctacacct gttagcacac
1921 ctgcgacaag tgggcagata aaggtactgt atgctaacaa ggagacaaac agcactacaa
1981 acacgataag gccgtggttg aaggtagtga atagtggtag cagtagcata gatttgagca
2041 gggtaacgat aaggtactgg tacacggtag atggtgagag ggcacagact ggatatcaga
2101 ctgggcacag ataggagcaa gcaatgtcaa cattcaagtt tgtgaagctg agagt
//GenBank-Updates@genbank.bio.net (03/30/91)
LOCUS CDCMANABD 2155 bp ds-DNA BCT 30-MAR-1991
DEFINITION C.saccharolyticum beta-D-mannanase (manA) gene, complete cds.
ACCESSION M36063 M37147
KEYWORDS 1,4-beta-D-mannan mannanohydrolase; beta-D-mannanase.
SOURCE C.saccharolyticum DNA, clone lambda-NZP2.
ORGANISM Caldocellum saccharolyticum
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 2155)
AUTHORS Luethi,E., Jasmat,N.B., Grayling,R.A., Love,D.R. and Bergquist,P.L.
TITLE Cloning, sequence analysis, and expression in Escherichia coli of a
gene coding for a beta-mannanase from the extremely thermophilic
bacterium Caldocellum saccharolyticum
JOURNAL Appl. Environ. Microbiol. 57, 694-700 (1991)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence kindly submitted
by E.Luethi, 03-JUL-1990.
Author address: E.Luethi
University of Auckland
Department of Cellular and Molecular Biology
Private Bag
Auckland
NEW ZEALAND
email:SYSTEM@CELVAX.AUKUNI.AC.NZ
FEATURES Location/Qualifiers
CDS <1..685
/label=ORF1
/codon_start=2
RBS 818..830
/note="manA ribosome binding site"
CDS 841..1881
/EC_number="3.2.1.78"
/product="beta-D-mannanase"
/codon_start=841
sig_peptide 841..963
/product="beta-D-mannanase"
/codon_start=841
mat_peptide 964..1878
/product="beta-D-mannanase"
/codon_start=964
BASE COUNT 721 a 334 c 590 g 510 t
ORIGIN
1 gggtagcaat acatggtttg gattccaggc atggtcgatg cagagggtag cggagtatta
61 ctatgtgaca ggagataaag atgcaggggc actgcttgag aagtgggtaa gctggataaa
121 gagtgtagtg aagttgaata gtgatggtac atttgcgata ccatcgacgc ttgattggag
181 tgggcagcca gacacatgga atgggacata tacaggtaat ccgaacttgc atgtgaaggt
241 agtagattat gggacggatt taggaataac ggcatcactt gcgaatgcac tactttatta
301 cagtgcaggg acgaagaagt atggggtatt tgatgaggaa gcgaagaatt tagcgaagga
361 attgctggac aggatgtgga agttatacag ggatgagaaa ggtttatcgg cgccagagaa
421 gagagcggac tacaagaggt tctttgagca agaggtatac attccggcag gatggacagg
481 gaagatgccg aatggagatg taataaagag cggagttaag tttatagaca taaggagcaa
541 gtacaaacaa gatcctgatt ggccgaagtt agaggcggca tacaagtcag ggcaggtacc
601 ggagttcaga tatcacaggt tctgggcaca gtgtgacata gcaattgtta atgcaacata
661 tgaaattctg ttcggtaatc aataatgagt aggtaaatgg aaatttagcg gggtggcaca
721 tctataagtt tggtgtgctg cctcgctaaa atcctgtatg gaagtgttcg aaaaatagta
781 caaaaaaatg gcgaggtaaa atggcaaaaa tgcataaaaa agagggggtt aggttttaag
841 atgagactaa aaacaaaaat aagaaagaaa tggttgagtg tactatgtac agttgttttt
901 ttattgaaca ttttgtttat agcaaatgta acgattttac ccaaagttgg tgcggctaca
961 tctaatgatg gagtagtgaa gatagatacg agtacgttaa taggaaccaa tcacgcacat
1021 tgctggtaca gagatagact tgatacggca ttgcgtggaa ttaggtcatg gggtatgaac
1081 tctgtgaggg tagtgctgag taatggttac cgatggacga agataccagc aagtgaagtg
1141 gcaaatatta tatcattgtc aagaagtctt ggtttcaaag ctattatatt agaagtgcac
1201 gacacaacag gatatggaga agatggggca gcatgttcat tggcacaagc agtggaatat
1261 tggaaggaga taaagagcgt attagacggt aacgaagatt ttgtaattat aaacattggt
1321 aatgagccgt atgggaacaa taactatcaa aactgggtta atgacacgaa gaacgctata
1381 aaagcactta gagatgcagg attcaagcac acgataatgg tggatgcgcc gaactggggt
1441 caggattggt ctaatactat gagagataat gcccagagca taatggaagc agatccgctg
1501 cgcaatttgg tattttcgat tcatatgtat ggcgtataca atacagcaag caaggtcgaa
1561 gagtacatca aatcatttgt tgataagggg ttaccattgg ttattggaga atttggacat
1621 cagcacacag atggtgaccc tgatgaagaa gctattgtca ggtatgcaaa acagtacaag
1681 ataggattat ttagttggtc gtggtgtgga aattcgagct atgttgggta tttggacatg
1741 gtaaacaact gggaccccaa taatccaact ccatggggac aatggtataa aactaatgca
1801 attggtacat cttctacacc aacacctacg tcgacagtaa cgccaacgcc acccccacgc
1861 caacaccaac accgacagta acagcgacac caacaccaac acctacacct gttagcacac
1921 ctgcgacaag tgggcagata aaggtactgt atgctaacaa ggagacaaac agcactacaa
1981 acacgataag gccgtggttg aaggtagtga atagtggtag cagtagcata gatttgagca
2041 gggtaacgat aaggtactgg tacacggtag atggtgagag ggcacagact ggatatcaga
2101 ctgggcacag ataggagcaa gcaatgtcaa cattcaagtt tgtgaagctg agagt
//