GenBank-Updates@genbank.bio.net (03/30/91)
LOCUS STMCEFF 954 bp ds-DNA BCT 30-MAR-1991 DEFINITION Streptomyces clavuligerus deacetylcephalosporin C synthetase gene, 5' end. ACCESSION M63809 M37186 KEYWORDS beta-lactam hydroxylase; cefF gene; deacetylcephalosporin C synthetase. SOURCE Streptomyces clavuligerus DNA. ORGANISM Streptomyces clavuligerus Prokaryota; Bacteria; Firmicutes; Streptomycetaceae. REFERENCE 1 (bases 1 to 954) AUTHORS Kovacevic,S. and Miller,J.R. TITLE Cloning and sequencing of the beta-lactam hydroxylase gene (cefF) from Streptomyces clavuligerus: Gene duplication may have led to separate hydroxylase and expandase activities in the Actinomycetes JOURNAL J. Bacteriol. 173, 398-400 (1991) STANDARD full staff_entry FEATURES Location/Qualifiers CDS 1..954 /product="deacetylcephalosporin C synthetase" /gene="cefF" /codon_start=1 /partial BASE COUNT 155 a 343 c 321 g 135 t ORIGIN 1 atggcggaca cgcccgtacc gatcttcaac ctcgccgcac tgcgggaagg cgccgatcag 61 gagaagttcc gcgagtgcgt gaccgggatg ggggtcttct acctcaccgg gtacggcgcc 121 ggggataagg accaccggct ggccacggac acggcgatgg acttcttcgc gaacggcacc 181 gaggccgaga aggcggccgt gaccacggac gtcccgacca tgcggcgcgg ctactccgcg 241 ctggaggccg agagcaccgc ccaggtgacc aggaccggtt cctacacgga ctactcgatg 301 tccttctcca tgggcatctc gggcaacgtc ttcccctcgc cggagttcga gcgggtgtgg 361 acggagtact tcgacaagct ctacgcggcg gcccaggaga cggcgcggct ggtgctgacc 421 gcgagcggcg gctatgacgc ggagatcgtc ggaagcctgg acgagctgct ggacgccgac 481 cccgtgctgc ggctgcggta cttccccgag gtgcccgagc accggtccgc cgagcacgag 541 ccgcgccgga tggccccgca ctacgacctg tcgatcatca ccttcatcca ccagacgccg 601 tgcgccaacg gcttcgtcag cctccaggcc gagatcggcg gcgaactggt gagcctgccc 661 gtcgtggagg acgccgtcgt cgtgatgtgc ggcgcgatgg ccccgctggc gacccagggc 721 gcgctgcccg cgccccggca ccacgtccgg tcccccggcg ccggtatgcg cgagggcagc 781 gaccgcacgt cgagcgtctt cttcctgcgc cccacgaccg acttctcgtt ctcggtggcc 841 aaggcccgca gctacggcct cgccgtcgac ctcgacatgg agacggccac cttcggcgac 901 tggatcggca ccaactacgt caccatgcac gcgaagaacg agccgcaggc cgga //