GenBank-Updates@genbank.bio.net (04/03/91)
LOCUS TRCSIALA 2259 bp ss-mRNA INV 03-APR-1991
DEFINITION T.cruzi sialidase (SA85-1.1) mRNA, complete cds.
ACCESSION M62735
KEYWORDS neuraminidase; sialidase.
SOURCE T.cruzi (strain CL) trypomastigote and amastigote, cDNA to mRNA.
ORGANISM Trypanosoma cruzi
Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE 1 (sites)
AUTHORS Kahn,S., Colbert,T., Wallace,J., Hoagland,N. and Eisen,H.
TITLE The major 85 kD surface antigen of the mammalian stage forms of
Trypanosoma cruzi is a family of sialidases
JOURNAL Proc. Natl. Acad. Sci. U.S.A. (1991) In press
STANDARD full staff_review
FEATURES Location/Qualifiers
mRNA 1..2259
/gene="SA85-1.1"
/partial
CDS 1..2259
/EC_number="3.2.1.18"
/note="also known as neuraminidase"
/product="sialidase"
/gene="SA85-1.1"
/codon_start=1
BASE COUNT 521 a 504 c 706 g 528 t
ORIGIN
1 atgtcccggc gcgtgtttgc ttccgcggtg ctgctcctaa ttgtcgtgac gatgtgctgc
61 ggtggagctg cgaccgctca ggtgggaagt aatgctgatg cttcaactcc tgggtcggca
121 ttgacgggtg ccattgctgg agaggggagt tcttcagggg gtgttgaggg gctgcaacgg
181 gtcgatcttt ttgtgccgca gaagacgcag gtgctgccga agaaggggcc tgactcaagt
241 cggagggatt catttttttc accctctctc gtcagcgctg gtggagtaat tgctgctttt
301 gccgagggcc acataaatac caaaaacccc cataacgaat cagctaagcc gttttctgat
361 gttgttgcgg ggtacatcga ctctgcgtgg gaatggccca ctcttgttga aaaggtgagt
421 gaaagtacat ggcaagcaca cactgtgctt ggtaaagcgg agggaaagaa gagtttggat
481 gttgtgctcc gccccacaac caccacgaag ggcaataagg tgtttcttct tgcgggaagc
541 actgatttgt cctatgtaaa ttggagttgg agagagggta gcttggagct gaaactggtt
601 gtgggtgatg tcacgaagcc tacgagcagc gagccgactg aacgtatcaa atggggcgaa
661 atcaagtctc tgttaaacga gagtactata gctgctcaaa aaggtaaatt gacggagttc
721 cttgcttctg gtggctcggg tgttgttatg gaggatggca ccattgtgtt ttctctgatg
781 gcggtgaatg aaaaaaaaga tggcgttttc tccttgatca tttactcgaa ggacaacggc
841 agtacctggt cgctctctga gggcatttcc cctgcgaaat gcggtgcacc ccgcatcacc
901 gagtgggagg gatcacttct catgattgtt gattgcgaga atgaccagag ggtgtacgtg
961 tcgcgtgaca tggggacaac gtggacggag gccatcggga cactctcagg cgttgggtca
1021 actcacaatt gggagactat cggacggagg cttgctgtgg aagccctcat caccgtgacc
1081 attgagggca ggaaggtcat gctgtacaca cagagagggt acgccttggg ggaaacggaa
1141 acgaccagtc cgctttacct ttgggtcacg gacaacaacc gctcgttttt tgttggaccg
1201 gttggcatgg acaatgctgt gaagggggag cttgccggcg ccctgctgta ctcggatggc
1261 ggattgcatc ttttacaacg gagggacagt ggtgaagaca gtgtcatgtc gctttcccgc
1321 ctgacggagg aactgaagga aattaagtcc gtcctcagca cttggtcgca aaaggacgtt
1381 ttcttctcca gcttgtctat acccacggtg ggtctggtgg cggtattgtc cgatgcggcc
1441 ggtgatggga gatggtacga cgagtacctt tgtctgaatg cgacggtgac gaacgccacg
1501 aaggtcaaag atggatttca gttgacggag cctgactcca gggcagtatg gtctgtgaac
1561 attccggatg gtaatgtgcg tcatatatcc ttgagccaca acttcacact cgtggcgtcg
1621 gtgatcatcg aagaggctcc gagtgggaac acacctctcc tgactgcggt gttggttgac
1681 gctgggcccg agtatttcat gagactgtcg tacaccgcgg ataacaagtg gatgacgatg
1741 ctcaaggacg agaaaaaacc aacaacggaa agcaggcctt gggaggcggg aaaagaacac
1801 caagtggcac tcatgctgca aggcaacaag gcctctgtgt acgttgatgg tgagttgctg
1861 ggggaggaag aagtgccgtt aacaggtgaa aagccacttg agattttcgc gttttgcttt
1921 ggcgcgtgca aaattgatgg tgatgaggag gagtcaagtc cgaaggagat cggaaaaaaa
1981 cctcgtgtga cggtgacgaa cgtctttctg tacaaccgcc cactgaattc cactgaaatg
2041 cgtgcaatca aggacaggat ccccgttccg acgagagctc cagaaccaca agtgaaaatt
2101 gctcccaaac ctgccgcgcc tgctgcacct gctgggaatg aagaaacggc acgagaaacg
2161 ggtgatggtg gggcaaatgg tgatgctgtt tccgcgtacg ggagggtgct gctgccgctg
2221 ctttttctgc ttggactgtg gggccttgcg actgcgtga
//GenBank-Updates@genbank.bio.net (04/03/91)
LOCUS TRCSIALB 2305 bp ss-mRNA INV 03-APR-1991
DEFINITION T.cruzi sialidase (SA85-1.2) mRNA, 3' end.
ACCESSION M62736
KEYWORDS neuraminidase; sialidase.
SOURCE T.cruzi (strain CL) trypomastigote and amastigote, cDNA to mRNA.
ORGANISM Trypanosoma cruzi
Eukaryota; Animalia; Protozoa; Sarcomastigophora; Mastigophora;
Kinetoplastida; Trypanosomatina; Trypanosomatidae.
REFERENCE 1 (sites)
AUTHORS Kahn,S., Colbert,T., Wallace,J., Hoagland,N. and Eisen,H.
TITLE The major 85 kD surface antigen of the mammalian stage forms of
Trypanosoma cruzi is a family of sialidases
JOURNAL Proc. Natl. Acad. Sci. U.S.A. (1991) In press
STANDARD full staff_review
FEATURES Location/Qualifiers
mRNA 1..2305
/gene="SA85-1.2"
/partial
CDS 1..2305
/EC_number="3.2.1.18"
/note="also known as neuraminidase"
/product="sialidase"
/gene="SA85-1.2"
/codon_start=2
/partial
BASE COUNT 545 a 543 c 712 g 505 t
ORIGIN
1 gtcgacattg acgggtgtca ttactgcaga ggggagcact tcagggggtg ttgaggggct
61 gcaacgggtc gatctatttg tgccgcagac gacgcaggtg ctgccgaaga aggggactga
121 atcaggtcgg agggattcat ttgtttcacc ctctcttgtc agtgctggtg gagtaattgc
181 tgcttttgcc gaaggtcaca taaatgccaa acccccccat aacgaatcaa ctaagccgtc
241 ttctgatgtt gttgcggagt acatcgactc tgcgtgggag tggcccactc ttgttgaaaa
301 ggtcggtaaa ggaacacgga ggtcacacac cgtgcttggc aaacgggagg gaaaggggat
361 tttggatgtt gtgcgccacc ccacaacaac gatgaagggc aataaggtgt ttcttcttgt
421 gggaagcact gccttgtccc ctgtaaatgg gagttggaaa gagggcagcc tggaactaaa
481 actggttgct ggtgaggtca cgaagccttc tgcgggcggc gagccgagtg agtggatcaa
541 atgggacgaa gttcaatcgc cggtgcagca gactacttta gctgctcaca aaggcaaatt
601 gacggagttt attgcttctg gtggctcagg tgttctgatg gaggatggca ccattgtgtt
661 ttctctgatg gcgatgaatg aaaaaaatga tggcgtttac tccatgatta tttactcgga
721 caacggcagt acctggtcgc tctctgagaa catctcacct gcgaattgca ctggcccccg
781 catcaccgag tgggagggat cacttatcat gattgttgac tgcgagagca gccagaagag
841 ggtgtacgag tcgcgtgaca tggggacaac gtggacggag gccatcggga cgcttccagg
901 cgtgtgggtc aactcacaat cggaagcctc tcaggattca agcctgcatg tggatgccct
961 catcaccgcg accattggga aaaggagggt catgctgtac acacagagag ggtacgccac
1021 ggaggaaaac gccaatccgc tctacctttg ggtgacggac aacaaccgct cgttttctgt
1081 tggaccggtt ggcatggaca gtgctgggaa gaaggaggtc gccagcaccc tgctgtactc
1141 ggatggcaag ttgcatcttt tacaacggag ggtcagtggt gaaggcagtg ccatttcact
1201 ctcccgcctg acggaggaac tgaaggaaat cgagtccgtc ctcagtactt ggtcgcaaaa
1261 ggacgttttc ttctccagct tttctatacc cacggcgggt ctggtggcag ttttgtcaaa
1321 cgcatcggcc agtgacgaca cgtggaacga cgagtacctt tgcctgaatg cgacggtgac
1381 gaacgccacg aaggtcaagg atgggtttct gttgacggag actgactcca gggtaatgtg
1441 gcctgtgaac actcggggtg ataatgtgcg tcatgtgttt ttgaaccaca acttcacact
1501 tgtggcgtcg gtgaccatcg aagaggctcc gagtgagaaa actcctctcc tgactgcgct
1561 gttgggtgac gctgagcccc cgtatttcat gagactgtcg tacaccgcgg ataacaagtg
1621 ggagacgatt tccaaaggcg ataaaaaact aacaacggaa agcaggcctt gggtgccgaa
1681 gaaagaacac caagtggcac tcatgctgca aggcaacaag gcctctgtgt acattgatgg
1741 tgagtcgctg ggggaagaag cgccgttaac agttgagaca ccacttgagc cctttgggtt
1801 ttgctttggc gcgtgcgact ttgatgatga tgatgatggt ggtgatgatg atgatgagga
1861 ggacagtcag gaggagtcca gtccgaagga gtccagtccg gagaagatcg gcaaaaaacc
1921 tcatgtgacg gtgacgaacg tctttctgta caaccgccca ctgaatccca ctgagatgcg
1981 tgcaatcaag gacaggatcc ccgtttcgac gagagctcca gaaccacaag tgaaaattgc
2041 tcccaaacct gtcgcacctg ctgcacctgc tgcacctgcg gtgcctggcc cacgggaagt
2101 accagcagca ccggggagga caactgtggg gagaaccgcc aatacccaac atgcgccagc
2161 gggacgcttg acttctgctg ggaatgaggg gacggcacga gaaagaggtg atggtggggc
2221 aaatggtgat gctggttccg cgtacgggag ggtgctgctg ccgctgctgc ttctgctggg
2281 actgtggggc tttgcgactg cgtga
//