GenBank-Updates@genbank.bio.net (04/03/91)
LOCUS BUTAMYAA 3523 bp ds-DNA BCT 03-APR-1991
DEFINITION B.fibrisolvens alpha-amylase (amyA) gene, complete cds.
ACCESSION M62507
KEYWORDS alpha-amylase.
SOURCE B.fibrosolvens (strain H17c) DNA.
ORGANISM Butyrivibrio fibrisolvens
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Anaerobic gram-negative straight, curved and helical rods;
Bacteroidaceae.
REFERENCE 1 (bases 1 to 3523)
AUTHORS Rumbak,E., Rawlings,D.E., Lindsay,G.G. and Woods,D.R.
TITLE An '-amylase from the ruminal bacterium Butyrivibrio fibrisolvens
H17c: Cloning, nucleotide sequence and enzymatic characterization
JOURNAL Unpublished (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 212..3142
/EC_number="3.2.1.1"
/product="alpha-amylase"
/gene="amyA"
/codon_start=212
BASE COUNT 1122 a 622 c 831 g 948 t
ORIGIN
1 atcgatggtt aaaggttaac tatgcaaatc ttacaaaaac gtttccggaa attataaaac
61 tttcaacaat tgacggtaat agtgccgaaa actataattt agttatcaag cagttaatcg
121 gttaacgcta atatgtttat tagcaaaaat gtaactatcg tcacgcagcc tacagctgca
181 aattcagttc cacactagat ttggaggttg tatgaaaagg ggaaaattgt ggggcagatt
241 agtatctgcg gcaggcctta gcttgtctat ttttttgagt tcgattggaa acgtttccac
301 tgcatacgcg atggaaagta atgatgcttt ggtccttgat gagaccaaag agaacactga
361 aagtgctact gatgcatcat ctaatgaagc gtcagatgca gaagcagata atgacacaga
421 tgaagcgata acagatgctt caagcaagga actttcagct gaaaatgatg gagcttcaga
481 atcagacagt tcatttgatg aatatgatca tactgctttg ccagaaactg atgagataac
541 agtaactgcg gctggagaac tttctactgc aaaggctgag ctttatacac tggcaccaag
601 agaggccagg gaagcagata acagccttgt tacaagagat agtattcatg atggagcaat
661 ccttcatgca ttttgctgga gctttaatac tatagctgat aatatggcag atattgcaga
721 tgccggatat acagctgttc agacatctcc gatcaatgaa tgtctttcaa ctaatcccgg
781 tatgaatctg catggtcctg atggaatgtg gtattaccac tatcagccaa cagactgggt
841 tattggtaac tatcagctcg gaagccgtga tgaattcaag cacatgtgcg atgttgccga
901 tgagtatggg gttgctgtca ttgtagatat ccttccaaac catacaactc cttctacagg
961 tagtattgcc aaggctctta tggaagctgc tggcggaagt gatgctcttt accacgcaac
1021 aggtaagata ggcggaggct atacagacag attagagctt acttactatt caatgggagg
1081 acttcctgat gtagatacag agaatacagg attccaacag tacttctatg aattccttaa
1141 agactgcgta tatctcggcg cagatggatt cagaattgat actgccaagc acatttcact
1201 tcctgatgat cctgttcctt ctgattactc agacgctggc agaaacactt tttatccaaa
1261 catgagagag gctcttaatg agtattcaga agaagtagga acaaagagct acgatgaact
1321 ctttgtctat ggagaagtac ttcagggaac aaatgacaga cttgcagcat atcagcagta
1381 tattggcgga acaactgcca gcaactatgg ctcaagcctt agatctgctc tttcaagcgg
1441 aaatctttct gtaaacagac ttttggatta tcagatttat gatgatacag cttatgggtc
1501 aacttatact gcagatacag aaaagcttgt tacctgggtt gagtctcatg acaactacat
1561 gaacgattct gagagctgct ggaagtctat tgatgacgat atggtcatca tgggctggtc
1621 aattatcgca gcaagagatg caggaacacc tttgttcttt agcagaccta acaacagctc
1681 agcagagaac ccatatggag ataaccttat tggtgcagca ggaagcccta tctataaggc
1741 acctgaagtc aaggcggtta atcttttccg tgaaaagatg ggcgaagctg atgaatatct
1801 ttcaaatccg ggcggaaata tacagacact tatgattgaa agatataacg atacagttca
1861 gggagctgta atcgtaaatg cagctcagac cagaacaact atcagcacag agacacattt
1921 atcagatggc atctatcctg atcaggttga aggaagcaat tctgtatttc ttgtaaagga
1981 tggtgtgctc agcggatctg ttgagggcga gggagtagta gttctgtctg agaaaatgga
2041 tggaacaggt aaggttgttt ctttctacaa caataagaac tggaatggtg ttgtagcaag
2101 agttgataat gcagaagaaa cacttgatac aattgatgaa aatgatggat ggttccaggt
2161 aactgttctt gatgatgagt tcaccataag atttgagagt gcagatggaa aagaggtttc
2221 tccagagttt cagattacag cagaaagcgg aacatttgct actcctgaca gctcagagct
2281 ttactattct aaggctgaag ctgaagaagg acttggaatt catacatatc ctgtatattt
2341 ctttaacact gaaaactggg gcagcgtata tacatatgga tggcttgacg gaggagcaca
2401 gctctttgga ggatggccgg gaacagttgc tgtaaatgaa ggttcaggct ggtatagagc
2461 agatgttaag actaccggtg agattacagc atttaatctt atctttaata atggaaacgg
2521 tattcagact gtaaatgtag agggcataac accggatagc aaggatattt atcttgcggt
2581 agatgcagaa aagtcaaatg gtcagcttat tgtaaacaga tatgaagata aggaatctgc
2641 agagaaggca cttggggtat ccggatcata tacaacagct tatttctata atacagaagg
2701 ctgggacaag gtttgtgcat atacatgggg cgcaacagct cttggagatt ggccgggtaa
2761 agaactgact caggatgagg atggctggta cagcgtagtt cttcctgccg gtcctagcga
2821 agatcttaac attattttca acaatggaaa taatggcaag cagacaaatg acatgaagat
2881 ttctgatatg aaatacagat ttatcctgaa taatggtatt tcctaccaga aatatggctc
2941 caaaaaggat gctatggaag ctattgccgg tgccggagat gttacatatg agacagttta
3001 tttctataac gaaaaagctg atgatgcaaa ctggaagaat gtatatctct atgtatttgg
3061 cggaacagat ggcgaataca acaaccttgt aggcacatgg cctggtaagc ttatggaaaa
3121 ggaagaggac agcaatggtt aagagcagag gtcccttcca aagctcttga aagcggaact
3181 cttacatata tctttaacaa tggaaatggt acgcagcttg atgataacaa gaatatcaca
3241 agcacaaaga actatttcac atttagtagc agagacagct ttgctagtaa ggaagaggtt
3301 tattcattcc ttggtatatc tacagatgag ccttctgctc cggaagagcc tcaggagcct
3361 gaagctactc ttaccaagaa gtatggtaaa tactatcttg tgacagaaga tggtgagaag
3421 cttacaggat tccatgaagt tgatggtatt ctcagatact ttgctgaaaa tactggtgta
3481 atggctataa acaagtgggt tactgtagga gataacaagt aca
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