GenBank-Updates@genbank.bio.net (03/30/91)
LOCUS ENTNFSI 1420 bp ds-DNA BCT 30-MAR-1991
DEFINITION Enterobacter cloacae oxygen-insensitive NAD(P)H nitroreductase
(nfsI) gene, complete cds.
ACCESSION M63808 M37085
KEYWORDS nfsI gene; oxygen-insensitive NAD(P)H nitroreductase.
SOURCE Enterobacter cloacae (strain 96-3, ATCC 43560) DNA, clone pCB9.
ORGANISM Enterobacter cloacae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1420)
AUTHORS Bryant,C. and DeLuca,M.
TITLE Purification and characterization of an oxygen-insensitive NAD(P)H
nitroreductase from Enterobacter cloacae
JOURNAL J. Biol. Chem. 266, 4119-4125 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
repeat_region 170..189
/rpt_type=inverted repeat
RBS 278..284
/gene="nfsI"
CDS 288..941
/product="oxygen-insensitive NAD(P)H nitroreductase"
/gene="nfsI"
/codon_start=288
BASE COUNT 342 a 395 c 345 g 338 t
ORIGIN
1 ctatcgcggg atagagccgg ctaccatctg agtaaaaagc actggatctc cctttacggc
61 accgacgacg tgacgccgga actggttacc gacctcatta atgattcgtg gaatctggtg
121 gtggataagc tgccgaaaaa agaccagaag tggatccgtc cggtctgatt gttcgtctga
181 gacgaacaga ttaagcgctc gaattgcact tatctttttc cgcgtcccgc tgtaatctgc
241 tttcctttcg cgcctcgagc gggcgaattt catcaccagg agttgttatg gatatcattt
301 ctgtcgccct gaaacgccac tctaccaagg cgttcgacgc aagcaaaaaa ctgaccgcgg
361 aagaagcgga aaaaatcaaa accctgctgc agtacagccc gtccagcacc aactcccagc
421 cgtggcactt cattgtagcc agcaccgagg aaggaaaagc gcgcgtggcg aagtccgctg
481 cgggcaccta tgtgttcaac gaacgcaaaa tgctggatgc ttcccacgtg gtggtgttct
541 gcgcgaaaac cgcgatggat gacgcctggc tggagcgcgt cgtggatcag gaagaggccg
601 atggccgttt caacacgccg gaagccaaag ccgcaaacca taagggccgc acctacttcg
661 ccgacatgca ccgcgtggat ctgaaagatg acgaccagtg gatggcgaag caggtttacc
721 tgaacgtcgg caacttcctg ctgggcgtgg gcgcgatggg tctggacgcg gtaccaattg
781 aaggtttcga cgccgctatt ctcgacgaag agtttggcct gaaagagaaa ggcttcacca
841 gcctggtggt ggtaccggtt gggcaccaca gcgtggaaga tttcaacgcc acgctgccga
901 aatctcgcct gccgctgagc acgattgtga ccgagtgctg atctcctctg gcacgctggg
961 ttccggcgtg cctgttttca atacagcata ctctaaggat gctacaccct atacgctctg
1021 cgctgagcat gcgtcagacc atcatgttct cgccattacg ccctcctctc gctgaagcgt
1081 ttctctcagc gtggaacatt caggttctca ccctaatttt gtcgttgtgt atcgattgcc
1141 ctcgttacag tcaaataatt tgtaacagca cagaaatacc cacatcagaa atttcgcata
1201 catttttaat taataagcac cccattccct ttcatatcaa caaattacac ccctcacccc
1261 tccaatcaat aaccctcaaa atatccccat tatttttctc acgtcacgcc aaatattcca
1321 cactgataga gcattaatta tttcgctctt tgcaggaatt gttacatgtc tattttagga
1381 ttcggggttc cgcatgtttt tataggccgc caccgcggtg
//GenBank-Updates@genbank.bio.net (04/04/91)
LOCUS ENTNFSI 1420 bp ds-DNA BCT 04-APR-1991
DEFINITION Enterobacter cloacae oxygen-insensitive NAD(P)H nitroreductase
(nfsI) gene, complete cds.
ACCESSION M63808 M37085
KEYWORDS nfsI gene; oxygen-insensitive NAD(P)H nitroreductase.
SOURCE Enterobacter cloacae (strain 96-3, ATCC 43560) DNA, clone pCB9.
ORGANISM Enterobacter cloacae
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (sites)
AUTHORS Bryant,C. and DeLuca,M.
TITLE Purification and characterization of an oxygen-insensitive NAD(P)H
nitroreductase from Enterobacter cloacae
JOURNAL J. Biol. Chem. 266, 4119-4125 (1991)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 1420)
AUTHORS Bryant,C.P., Hubbard,L. and McElroy,W.D.
TITLE Cloning, nucleotide sequence, and expression of the nitroreductase
gene from Enterobacter cloacae.
JOURNAL J. Biol. Chem. 266, 4126-4130 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
repeat_region 170..189
/rpt_type=inverted repeat
RBS 278..284
/gene="nfsI"
CDS 288..941
/product="oxygen-insensitive NAD(P)H nitroreductase"
/gene="nfsI"
/codon_start=288
BASE COUNT 342 a 395 c 345 g 338 t
ORIGIN
1 ctatcgcggg atagagccgg ctaccatctg agtaaaaagc actggatctc cctttacggc
61 accgacgacg tgacgccgga actggttacc gacctcatta atgattcgtg gaatctggtg
121 gtggataagc tgccgaaaaa agaccagaag tggatccgtc cggtctgatt gttcgtctga
181 gacgaacaga ttaagcgctc gaattgcact tatctttttc cgcgtcccgc tgtaatctgc
241 tttcctttcg cgcctcgagc gggcgaattt catcaccagg agttgttatg gatatcattt
301 ctgtcgccct gaaacgccac tctaccaagg cgttcgacgc aagcaaaaaa ctgaccgcgg
361 aagaagcgga aaaaatcaaa accctgctgc agtacagccc gtccagcacc aactcccagc
421 cgtggcactt cattgtagcc agcaccgagg aaggaaaagc gcgcgtggcg aagtccgctg
481 cgggcaccta tgtgttcaac gaacgcaaaa tgctggatgc ttcccacgtg gtggtgttct
541 gcgcgaaaac cgcgatggat gacgcctggc tggagcgcgt cgtggatcag gaagaggccg
601 atggccgttt caacacgccg gaagccaaag ccgcaaacca taagggccgc acctacttcg
661 ccgacatgca ccgcgtggat ctgaaagatg acgaccagtg gatggcgaag caggtttacc
721 tgaacgtcgg caacttcctg ctgggcgtgg gcgcgatggg tctggacgcg gtaccaattg
781 aaggtttcga cgccgctatt ctcgacgaag agtttggcct gaaagagaaa ggcttcacca
841 gcctggtggt ggtaccggtt gggcaccaca gcgtggaaga tttcaacgcc acgctgccga
901 aatctcgcct gccgctgagc acgattgtga ccgagtgctg atctcctctg gcacgctggg
961 ttccggcgtg cctgttttca atacagcata ctctaaggat gctacaccct atacgctctg
1021 cgctgagcat gcgtcagacc atcatgttct cgccattacg ccctcctctc gctgaagcgt
1081 ttctctcagc gtggaacatt caggttctca ccctaatttt gtcgttgtgt atcgattgcc
1141 ctcgttacag tcaaataatt tgtaacagca cagaaatacc cacatcagaa atttcgcata
1201 catttttaat taataagcac cccattccct ttcatatcaa caaattacac ccctcacccc
1261 tccaatcaat aaccctcaaa atatccccat tatttttctc acgtcacgcc aaatattcca
1321 cactgataga gcattaatta tttcgctctt tgcaggaatt gttacatgtc tattttagga
1381 ttcggggttc cgcatgtttt tataggccgc caccgcggtg
//