GenBank-Updates@genbank.bio.net (04/04/91)
LOCUS ECOICIA 1815 bp ds-DNA BCT 04-APR-1991
DEFINITION E.coli chromosome initiation inhibitor (iciA) gene, complete cds.
ACCESSION M62865
KEYWORDS chromosome initiation inhibitor; iciA gene.
SOURCE E.coli (strain W3110) DNA, (Kohara library) clone IA2
('miniset'-set No. 471).
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1815)
AUTHORS Thoeny,B., Hwang,D.S., Fradkin,L. and Kornberg,A.
TITLE IciA, an Escherichia coli gene encoding a specific inhibitor of
chromosomal initiation of replication in vitro
JOURNAL Unpublished (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 650..1543
/function="Inhibitor of oriC replication in vitro"
/product="chromosome initiation inhibitor"
/gene="iciA"
/codon_start=650
BASE COUNT 468 a 486 c 440 g 421 t
ORIGIN 62.8 min. on K-12 map; kb coordinate 3974.
1 gtcgacttcg ttgagatcaa aaacgtgaat gccgaggctt ttcagttttt cagtggaagc
61 atctgaactg gaaacggccc cttcaatctg gcctttcatt gtaccgagcg cgtcaataaa
121 gtgtgcggcg gtggaacctg tacctacacc aacaatggtg ccgggctgaa catactgaag
181 tgccgcccat cctactgctt ttttcaattc atcctgcgtc atgatcgttt cgcctgtggt
241 atgaaatttc acacgcatta tatacaaaaa aagcgattca gaccccgttg gcaagccgcg
301 tggttaactc atccataaaa tatcgcgcaa tggcaggcat cccctttcgc cccgcaaata
361 aagcatacaa cggtctgggt atgccgctcc acggtgcaaa caggcgcacc agttcaccgt
421 tcgcaagccc ctgtttacag gcaaattgag gcaataacgc cacgccattc aacacaacca
481 gggcgcgaac tcgctgagcg aaatgcatta gcgcaaatcc ctgatctttt aagctgcgct
541 tttaaccacg gatacaaatg ttacccgccg acggcttcgg tatatgcaac ctgacacaaa
601 attgtgtcat agtgcaggaa aaagcattta ccaggagcag acaacagcaa tgaaacgccc
661 ggactacaga acattacagg cactggatgc ggtgatacgt gaacgaggat ttgagcgcgc
721 ggcacaaaag ctgtgcatta cacaatcagc cgtctcacag cgcattaagc aactggaaaa
781 tatgttcggg cagccgctgt tggtgcgtac cgtaccgccg cgcccgacgg aacaagggca
841 aaaactgctg gcactgctgc gccaggtgga gttgctggaa gaagagtggc tgggcgatga
901 acaaaccggt tcgactccgc tgctgctttc actggcggtc aacgccgaca gtctggcgac
961 gtggttgctt cctgcactgg ctcctgtgtt ggctgattcg cctatccgcc tcaacttgca
1021 ggtagaagat gaaacccgca ctcaggaacg tctgcgccgc ggcgaagtgg tcggcgcggt
1081 gagtattcaa catcaggcgc tgccgagttg tcttgtcgat aaacttggtg cgctcgacta
1141 tctgttcgtc agctcaaaac cctttgccga aaaatatttc cctaacggcg taacgcgttc
1201 ggcattactg aaagcgccag tggtcgcgtt tgaccatctt gacgatatgc accaggcctt
1261 tttgcagcaa aacttcgatc tgcctccagg cagcgtgccc tgccatatcg ttaattcttc
1321 agaagcgttc gtacaacttg ctcgccaggg caccacctgc tgtatgatcc cgcacctgca
1381 aatcgagaaa gagctggcca gcggtgaact gattgactta acgcctgggc tatttcaacg
1441 acggatgctc tactggcacc gctttgctcc tgaaagccgc atgatgcgta aagtcactga
1501 tgcgttactc gattatggtc acaaagtcct tcgtcaggat taatccatca aataatgcct
1561 gatagcacat atcaggcgtt gtcctcactt ctttttgtat tccttgaatc acatcacaaa
1621 atagacaaat ctcaggcggc aaaaaacgac gtctgaatgc attttttttg ctggcgacaa
1681 acccacgtaa aaagctcacc gtaggcgcaa ataccctcat tttgattgcg ttttacggag
1741 caaataatgt ctaacgtgca ggagtggcaa cagcttgcca acaaggaatt gagccgtcgg
1801 gagaaaactg tcgac
//