GenBank-Updates@genbank.bio.net (04/06/91)
LOCUS KAEHUTCU 250 bp ds-DNA BCT 06-APR-1991
DEFINITION K.aerogenes histidine utilization proteins hutC (3' end) and hutU
(5' end) genes.
ACCESSION M19665
KEYWORDS histidine utilization protein.
SOURCE K.aerogenes DNA.
ORGANISM Klebsiella aerogenes
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 250)
AUTHORS Nieuwkoop,A.J., Baldauf,S.A., Hudspeth,M.E. and Bender,R.A.
TITLE Bidirectional promoter in hut(P) region of the histidine
utilization (hut) operons from Klebsiella aerogenes
JOURNAL J. Bacteriol. 170, 2240-2246 (1988)
STANDARD full staff_entry
REFERENCE 2 (sites)
AUTHORS Osuna,R., Boylan,S.A. and Bender,R.A.
TITLE In vitro transcription of the histidine utilization (hutUH) operon
from Klebsiella aerogenes
JOURNAL J. Bacteriol. 173, 116-123 (1991)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [J. Bacteriol. 170,
2240-2246 (1988)] kindly provided
by R.Bender, 08-JUL-1988.
FEATURES Location/Qualifiers
CDS <1..81
/note="histidine utilization protein hutC (AA at 1)"
/codon_start=1
mRNA 100..>250
/note="hut mRNA"
misc_binding 100..120
/note="CAP-cAMP binding site"
misc_feature complement(129..157)
/note="Pc2 promoter"
misc_feature 162..183
/note="operator"
CDS 224..>250
/note="histidine utilization protein hutU"
/codon_start=224
BASE COUNT 63 a 68 c 55 g 64 t
ORIGIN 151 bp upstream of BstNI site.
1 tcgacaaccc acatcgtctc ccacgcgcgc ctgctgttcc ccggtagccg atatcgtctg
61 caggggcgct ttggctcctg atccacgctg gcgtcagcaa aacgtgattg ctgacgcaat
121 ataacaaaat tgtatcattt ctgttaaatc ctggcttgcg catgcttgta tagacaagta
181 tatgtatcta cgtaaacaac gtttattgtc aggagaaacc ccgatgtcgc aaagcaaata
241 tcgccagctg
//