GenBank-Updates@genbank.bio.net (04/06/91)
LOCUS STYPUTPA 546 bp ds-DNA BCT 06-APR-1991
DEFINITION S. typhimurium put regulatory region (proline utilization).
ACCESSION X12569
KEYWORDS putA gene; putP gene.
SOURCE Salmonella typhimurium.
ORGANISM Salmonella typhimurium
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 546)
AUTHORS Hahn,D.R., Myers,R.S., Kent,C.R. and Maloy,S.R.
TITLE Regulation of proline utilization in Salmonella typhimurium:
Molecular characterization of the put operon, and DNA sequence of
the put control region
JOURNAL Mol. Gen. Genet. 213, 125-133 (1988)
STANDARD simple automatic
REFERENCE 2 (sites)
AUTHORS De Spicer,P.O., O'Brien,K. and Maloy,S.
TITLE Regulation of proline utilization in salmonella typhimurium: a
membrane-associated dehydrogenase binds DNA in vitro
JOURNAL J. Bacteriol. 173, 211-219 (1991)
STANDARD full staff_review
COMMENT P1putP, P2putP and P3putP are potential put promoters for the putP
gene, comprising -35 and -10 regions. PputA is the potential
promoter region for the putA gene.
EMBL features not translated to GenBank features:
key from to description
RBS 56 52 (C) Shine-Dalgarno sequence
PRM 247 220 (C) pot. P3putP promoter region
SITE 271 289 cAMP receptor protein binding site
PRM 319 291 (C) pot. P2putP promoter region
SITE 360 380 cAMP receptor protein binding site
PRM 390 418 pot. PputA promoter region
SITE 416 442 put operator
PRM 477 449 (C) pot. P1putP promoter region
RBS 460 464 Shine-Dalgarno sequence
FEATURES Location/Qualifiers
CDS complement(<1..45)
/note="putP protein"
/codon_start=45
CDS 467..>546
/note="putA protein"
/codon_start=467
BASE COUNT 145 a 123 c 122 g 156 t
ORIGIN
1 aatatagaca cagaatgtca ccaacatcgg tgtgctaata gccatcaagg ttctccaaat
61 ttatttattg gcactaccgt agcgtgccgt ttttgtaccc tgccggaacg tggcaagggt
121 gttatgttta ccagggcgac cgtatcctgc cggaagcggt ggttattcac aatcgattta
181 acacaccatt tacatcaaat ttgattgcag agggatacta tttttctcca ggttgccact
241 ctctcacatt ttttcggttg caccttttaa aagtgttaac taccgcagag aaaataaatc
301 cagatggttt tctcgtcaga cactccctta tcttttttta acaattcatt catttttaag
361 cttgcaacgc aggtcacatt taacgcggtt gcacaagttg caacatggtg gatattttcc
421 gctaactatt ggttagcaga tagagcgaat aacaggagta gagggtatgg gaaccaccac
481 gatgggggtt aagctggacg acgcccacgc gcgaacggat caaatggccg cgtcgcgtat
541 cgatcg
//