GenBank-Updates@genbank.bio.net (04/06/91)
LOCUS CNVGENO 4701 bp ss-RNA VRL 06-APR-1991
DEFINITION Cucumber necrosis virus, complete genome.
ACCESSION M25270
KEYWORDS coat protein; complete genome; readthrough protein; replicase.
SOURCE Cucumber necrosis virus, cDNA to viral RNA, clone pCNV220A.
ORGANISM Cucumber necrosis virus
Viridae; ss-RNA nonenveloped viruses; Isometric ss-RNA viruses;
Tobacco necrosis viruses.
REFERENCE 1 (bases 1 to 4701)
AUTHORS Rochon,D.M. and Tremaine,J.H.
TITLE Complete nucleotide sequence of the cucumber necrosis virus genome
JOURNAL Virology 169, 251-259 (1989)
STANDARD simple staff_entry
REFERENCE 2 (sites)
AUTHORS Rochon,D.M. and Johnston,J.C.
TITLE Infectious transcripts from cloned cucumber necrosis virus cDNA:
Evidence for a bifuntional subgenomic mRNA
JOURNAL Virology 181, 656-665 (1991)
STANDARD full staff_review
COMMENT Draft entry and computer-readable sequence for [Virology 169,
251-259 (1989)] kindly submitted
by D.Rochon, 25-OCT-1989.
FEATURES Location/Qualifiers
CDS join(152..1039,1043..2608)
/note="replicase (put.; readthrough protein)"
/codon_start=152
exon 152..1039
/note="replicase (put.; readthrough protein)"
CDS 152..1042
/note="ORF p33"
/codon_start=152
misc_feature 1040..1042
/note="readthrough codon"
exon 1043..2608
/note="replicase (put.; readthrough protein)"
CDS 2628..3770
/note="coat protein"
/codon_start=2628
CDS 3800..4372
/note="ORF p21"
/codon_start=3800
CDS 3832..4353
/note="ORF p20"
/codon_start=3832
BASE COUNT 1228 a 1007 c 1291 g 1173 t 2 others
ORIGIN
1 nnaaattctc caggatttct caaccttggt tgtgctatct ggtgacttgc gcgtgttgtc
61 tgcgtagaga atttctctcc ttaaccaaaa ggggtttgaa gactgggtct accgcttgcg
121 ggataaattg taacttccag taaacgacga catggatacc atcaagagga tgctgtggcc
181 taagaaagaa atttttgttg gcacgttcgc gaccggagtg gaaagggaca cctcggtgga
241 catcttccaa ttagtgtgta gggttgtgtt gagatacatg aggacaggga aaatcgagaa
301 taacactgac agtctgggaa atttcatcgt tgaacttctc aagacagatt gtgcagccaa
361 atgggaatgg ttcatgaaac gcaggagggt tggtgattac gccaagtcac tcgccattgc
421 ttccatccca gttattccgt tgcttagcta tgccacgatg aagaagaccg ttgcgcttcg
481 ggcctttggt aatgaactct cgttcaacat cagggttcca aggccatcag tacctaagaa
541 aggcttgcta ctgcgactgg cggcaggact agcgttagca cccatttgcg cactagccat
601 gtacgctacc ctacccaggg aaaaactgtc ggtatttaag cttcgcactg aagcccgagc
661 acatatggag gatgaaaggg aagcgacgga ttgtttggtg gttgagccgg cacgggagct
721 taagggtaag gatggagagg atctcctcac tggcagtagg atgacaaagg taattgcgtc
781 cacaggacgc cctcgcagaa gaccttatgc agctaagatt gcacaggtag cgagagccaa
841 ggtgggttac ctgaagaaca ctcctgagaa tagattgata taccagagag tgatgatcga
901 gatcatggac aaagattgcg tcaggtatgt tgacagggat gtcatattac cattggctat
961 tggatgctgc tttgtctacc cggatggagt ggaggagtcg gccgcacttt ggggctcgga
1021 tgagtccctt ggtgtgaaat agggaggcct agtacgtcta cctggggttg tgacacagac
1081 caatcgagat atcccatctg gtgtgttact tccccaggaa gtgctagagg ttcgtgcagg
1141 acctcccaat gctaaggacc gtaacatatt catggttgcg ggttgcccat cacaggcgcg
1201 gttcttagta cacaatcatt gcctgaaaaa cctgaaaagg ggtcttgtgg agagagtttt
1261 ctgtgtcgag aggaacggtc aactcactcg cactccacaa cctaccaaag gagcctttgg
1321 acgtctttcc ccgttcagga aagcggtctg cgagaaggtc ggggttgccc accgtttggg
1381 gtatgatgga tttctatcat actacagtgg tgcgaagctc cgtacttaca cgcgggctgt
1441 ggagagtctg catatcacac ctgtctctga gagggatagt cacttgacta cctttgtcaa
1501 ggcagagaag atatcgacgg ccaaaggtga cccagcacct agggtgattc agcctcgaaa
1561 ccctaggtac aatgtggagc ttggaagata tctgcggcat atggaatcca aacttatgaa
1621 agctgtggat ggcgtatttg gtgaaaccac atgcattaaa ggttatactg ctgatgaggt
1681 ggggcagatt ttccgggata aatgggatag gtttaataaa cccgtcgcta tcggtctcga
1741 tgcctcacgg tttgatcaac actgttccgt ggaggccttg caatttgagc atggtttcta
1801 cagggccatg taccctggca ataagctttt gagcaagttg ttggactggc aactccataa
1861 caaagggaaa ggttatgtgc ctgacggcac aataacttac cgtaaggagg gttgccgcat
1921 gagtggggat ataaacacgt cacttggtaa ttatctttta atgtgtgcta tgatctacgg
1981 gtatatgcgc catttgggga tcaatgaata cagtctggcg aactgcgggg atgactgcgt
2041 tctcattgtg gagcgtagga atcttaagca gatacagggt acactgcctg agtatttcct
2101 aaatttgggt tatacgatga aggtggaacc acctgtattt caattggaag aggttgaatt
2161 ctgtcaggca cacccagtgc agtttcaagg tggttggaag atggtccgga acgtcagaac
2221 agccatgagc aaggatgttc actgtgttaa taatattagg gatttggcca caaggagggc
2281 gtggagtaat gctcagcatc atggcggggt tgcgcttact agcggtattc ctgttgtgga
2341 gaaattttac tccagattta cactctatga agtccctaag aagcaccagc gcatagacac
2401 agtgacaaat gtgcacaaat ggcgtggttc tggcggtgac tatgttgtga cccctgaggc
2461 aagggcgagc ttttgggctg cctttggact cacgggggat gagcagctgg cactagagga
2521 ccgattggac agatgggaaa tggatctttt tggagaggag ggtgttgacg cgcatgagcc
2581 cagcatcctt gactccgccg tagcatgacc aagcaaacac aaacacaatg gcactcgtaa
2641 gcaggaacaa caatatgcga acacttgcaa agttagccgc cccattggct acggcaggga
2701 cacgcacgat agtggacaat aaagaagcta tctggaatgg agtaaaatgg atctggggaa
2761 agttaccgaa aggcaagaaa ggtaagaatg gaaacggtgc tctcatagcc cacccacagg
2821 cttttcctgg ggctatcgcc gcgccaatct cttatgccta tgcggttaaa ggaaggaaac
2881 ctaggtttca aacagcaaaa ggatctgtgc gaataaccca tagagagtat gtgtcggttc
2941 tttccggcac taatggggag ttcctacgca acaacgggac tggaccgaac aacgatttca
3001 gcattaatcc gctgaaccct ttcttgttcc cgtggcttgt aaatattgcc gccaactttg
3061 atcagtacaa gttcaacagt ttgaggttcg agtatgtacc gcttgttaat acaacgacta
3121 atggccgtgt agctttatat tttgataagg atagcgagga ccctggcccc gatgataggg
3181 ctgcccttgc gaactatgct cacttgagtg agatatctcc ttgggcgata acaaagctta
3241 ccgtgcctac agacaatgtc aagcggttca tcagtgacac ctcgtcgggt gacccgaagc
3301 tgatcaacct tgggcagttt ggatgggtcg cttatagcgg ccctacagct gagttggggg
3361 atattttcgt agagtacaca gtagatctct ttgaggcaca accaacgtcg cctcttctgg
3421 aatcgttgtt ccgggagagc gctagtagtg tgcaaacgag aatgggttta ccttactttt
3481 ccctggaggt ggcttcggct accgaccttg tatggcaggc acgtgtgccc ggcacatatg
3541 tggttaccat tatcttcaac agtacagtcg gggggctcac accgtccatt tctggaggtg
3601 ggactattaa tagttccttt agtgtgagca ccgccggaag ctctgcatat gttgcaaaca
3661 tcactatacg tgtgaatgcc aacttgagtc tttctgggct cacgggagcg acaaacgcgc
3721 agctctttgc ggtgcgagca atcactgaaa atgcggtgca ggttgtgtaa attaggggct
3781 tcttgaatct aaccaattca tggatactga atacgaacaa gtcaataaac catggaacga
3841 gctatacaaa gaagcgacgc tagggaacaa gctaacagtg aacgttggga tggaagatgt
3901 ggaggtacca ttactccctt caaacttcct gacgaaagtc cgagtctcct tgagtggagg
3961 ctacataaca gtgaggagag tgaggataaa gatcatcccc ttggtttcaa ggaaagctgg
4021 agtttcggga aagttgtatt taagagatat ctcagatacg acgggacgga aacttcattg
4081 cacagaactc ttggatcttg ggaaagaaat tcggttaacg atgcagcatc tcgatttctc
4141 ggtgtcagcc agatcggatg tacctatagt attcggtttc gaggatcttg tctcacctta
4201 tctggagggt cgcgaactct tcagcgtctc attgagatgg caattcggac taagcgcaca
4261 atgttacagc ttaccccctg cgaagtggaa ggtaatgtat caagaagacg ccctcaaggc
4321 tctgaagcct tcgaaaataa agaaagcgag taagacagac tcttcagtct gacttggtgg
4381 aatcttgcga atttaactgt tactcttcat gggttccttc ccatacgatg acgagtcagg
4441 tcgggcccta tcttaggttt ggtcacctag gggacgggga tatggaaatc actttcgctt
4501 gctgtcagtc tagtggaaac acttagcttg caatgtgggt gtatgcctgg ataagtcgta
4561 tggatgctgg ccatgatgaa ttggatgcag tttaggacgt atagtggaaa tcttgccaga
4621 cacggttgat ctcaccctcc ggggggctat agagatcgct ggaacactac cggacaaccg
4681 gaacattgca gaaatgcagc c
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