GenBank-Updates@genbank.bio.net (04/10/91)
LOCUS ECORF1X 1441 bp ds-DNA BCT 10-APR-1991
DEFINITION E.coli RF-1 gene encoding peptide chain release factor 1, complete
cds.
ACCESSION M11519 M22626
KEYWORDS RF1 gene; peptide chain release factor 1.
SOURCE E.coli (strain RR1) DNA, clone pRF1-1.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1441)
AUTHORS Craigen,W.J., Cook,R.G., Tate,W.P. and Caskey,C.T.
TITLE Bacterial peptide chain release factors: Conserved primary
structure and possible frameshift regulation of release factor 2
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 82, 3616-3620 (1985)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 1441)
AUTHORS Lee,C.C., Kohara,Y., Akiyama,K., Smith,C.L., Craigen,W.J. and
Caskey,C.T.
TITLE Rapid and Precise Mapping of the Escherichia coli Release Factor
Genes by Two Physical Approaches
JOURNAL J. Bacteriol. 170, 4537-4541 (1988)
STANDARD full staff_review
COMMENT The precise transcription initiation site was not located.
Potential -35 and -10 regions were located at positions 258-263 and
283-288 respectively.
Draft entry and printed copy of the sequence for [Proc. Natl. Acad.
Sci. U.S.A. 82, 3616-3620 (1985)] was kindly
provided by W.J.Craigen, 04-OCT-1985.
FEATURES Location/Qualifiers
CDS 354..1436
/note="peptide chain release factor 1"
/codon_start=354
BASE COUNT 368 a 370 c 400 g 303 t
ORIGIN 133 bp upstream of HincII site.
1 aaagccgcag cggttgaggc ggaaactatt gtcgctcagg aaaccagcga atttatggcg
61 tggctgcgag cacaaagcgc cagcgaaacc attcgcgagt atcgcagcca gcagagcaag
121 ttcgcgatga gttaacgcca aagcgttagc ggcccttgag cagggcggcg acgcgcaagc
181 cattatgcag gatctggcat ggaaactgac taaccgcttc atccatgcgc caacgaaatc
241 acttcaacag gccgcccgtg acggggataa cgaacgcctg aatattctgc gcgacagctc
301 gggctggagt agcagtacat cattttcttt ttttacaggg tgcatttacg cctatgaagc
361 cttctatcgt tgccaaactg gaagccctgc atgaacgcca tgaagaagtt caggcgttgc
421 tgggtgacgc gcaaactatc gccgaccagg aacgttttcg cgcattatca cgcgaatatg
481 cgcagttaag tgatgtttcg cgctgtttta ccgactggca acaggttcag gaagatatcg
541 aaaccgcaca gatgatgctc gatgatcctg aaatgcgtga gatggcgcag gatgaactgc
601 gcgaagctaa agaaaaaagc gagcaactgg aacagcaatt acaggttctg ttactgccaa
661 aagatcctga tgacgaacgt aacgccttcc tcgaagtccg agccggaacc ggcggcgacg
721 aagcggcgct gttcgcgggc gatctgttcc gtatgtacag ccgttatgcc gaagcccgcc
781 gctggcgggt agaaatcatg agcgccagcg agggtgaaca tggtggttat aaagagatca
841 tcgccaaaat tagcggtgat ggtgtgtatg gtcgtctgaa atttgaatcc ggcggtcatc
901 gcgtgcaacg tgttcctgct acggaatcgc agggtcgtat tcatacttct gcttgtaccg
961 ttgcggtaat gccagaactg cctgacgcag aactgccgga catcaaccca gcagatttac
1021 gcattgatac tttccgctcg tcaggggcgg gtggtcagca cgttaacacc accggttcgg
1081 caattcgtat tactcacttg ccgaccggga ttgttgttga atgtcaggac gaacgttcac
1141 aacataaaaa caaagctaaa gcactttctg ttctcggtgc tcgcatccac gctgctgaaa
1201 tggcaaaacg ccaacaggcc gaagcgtcta cccgtcgtaa cctgctgggg agtggcgatc
1261 gcagcgaccg taaccgtact tacaacttcc cgcaggggcg cgttaccgat caccgcatca
1321 acctgacgct ctaccgcctg gatgaagtga tggaaggtaa gctggatatg ctgattgaac
1381 cgattatcca ggaacatcag gccgaccaac tggcggcgtt gtccgagcag gaataatgga
1441 a
//