GenBank-Updates@genbank.bio.net (04/10/91)
LOCUS ECORF2X 1441 bp ds-DNA BCT 10-APR-1991
DEFINITION E.coli RF-2 gene encoding peptide chain release factor 2, complete
cds.
ACCESSION M11520 M22625
KEYWORDS RF2 gene; peptide chain release factor 2.
SOURCE E.coli (strain RR1) DNA, clone pRF2-1.
ORGANISM Escherichia coli
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Facultatively anaerobic rods; Enterobacteriaceae.
REFERENCE 1 (bases 1 to 1441)
AUTHORS Craigen,W.J., Cook,R.G., Tate,W.P. and Caskey,C.T.
TITLE Bacterial peptide chain release factors: Conserved primary
structure and possible frameshift regulation of release factor 2
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 82, 3616-3620 (1985)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 1441)
AUTHORS Lee,C.C., Kohara,Y., Akiyama,K., Smith,C.L., Craigen,W.J. and
Caskey,C.T.
TITLE Rapid and Precise Mapping of the Escherichia coli Release Factor
Genes by Two Physical Approaches
JOURNAL J. Bacteriol. 170, 4537-4541 (1988)
STANDARD full staff_review
COMMENT An in-frame premature termination codon is located within the RF-2
coding region at positions 377-379. [Proc. Natl. Acad. Sci. U.S.A.
82, 3616-3620 (1985)] confirms that the sequence
presented is correct by sequencing a second independent isolate of
of the RF-2 gene and that the reading frame is correct by
sequencing the first 44 amino acids of the protein by automated
Edman degradation. These results imply a frameshift prior to the
termination codon. The sequence 'ctttga,' positions 374-379,
should be the site of the frameshift because peptide sequencing
confirms the presence of Leu as AA 25 [Proc. Natl. Acad. Sci.
U.S.A. 82, 3616-3620 (1985)]. For this entry, the
frameshift is shown by including a 1 bp intron at position 377.
The initiation site of transcription was not identified. However,
a potential -10 box is present at positions 247-252.
Draft entry and printed copy of the sequence for [Proc. Natl. Acad.
Sci. U.S.A. 82, 3616-3620 (1985)] was kindly
provided by W.J.Craigen, 14-OCT-1985.
FEATURES Location/Qualifiers
CDS join(302..376,378..1400)
/note="peptide chain release factor 2"
/codon_start=302
exon 302..376
/note="peptide chain release factor 2, exon 1
(frameshift)"
exon 378..1400
/note="peptide chain release factor 2, exon 2"
BASE COUNT 389 a 353 c 397 g 302 t
ORIGIN 56 bp upstream of AvaII site.
1 acagcggctg gtgggcgaac gtcatttgaa ggtgatggtc gaaccggtcg gcggcggtcc
61 actgctggat ggtattgctt ttaatgtcga taccgccctc tggccggata acggcgtgcg
121 cgaagtgcaa ctggcttata agctcgatat caacgagttt cgcggcaacc gcagcctgca
181 aattatcatc gacaatatct ggccaattta gcgtcatctt ctctataaaa aagagcgtgg
241 attgggtaca atcccgctct tatcaccgca ttttgactag ctcaataaaa gaaatcagac
301 catgtttgaa attaatccgg taaataatcg cattcaggac ctcacggaac gctccgacgt
361 tcttaggggg tatctttgac tacgacgcca agaaagagcg tctggaagaa gtaaacgccg
421 agctggaaca gccggatgtc tggaacgaac ccgaacgcgc acaggcgctg ggtaaagagc
481 gttcctccct cgaagccgtt gtcgacaccc tcgaccaaat gaaacagggg ctggaagatg
541 tttctggtct gctggaactg gctgtagaag ctgacgacga agaaaccttt aacgaagccg
601 ttgctgaact cgacgccctg gaagaaaaac tggcgcagct tgagttccgc cgtatgttct
661 ctggcgaata tgacagcgcc gactgctacc tcgatattca ggcggggtct ggcggtacgg
721 aagcacagga ctgggcgagc atgcttgagc gtatgtatct gcgctgggca gaatcgcgtg
781 gtttcaaaac tgaaatcatc gaagagtcgg aaggtgaagt ggcgggtatt aaatccgtga
841 cgatcaaaat ctccggcgat tacgcttacg gctggctgcg tacagaaacc ggcgttcacc
901 gcgtggtgcg taaaagcccg tttgactccg gcggtcgtcg ccacacgtcg ttcagctccg
961 cgtttgttta tccggaagtt gatgatgata ttgatatcga aatcaacccg gcggatctgc
1021 gcattgacgt ttatcgcacg tccggcgcgg gcggtcagca cgttaaccgt accgaatctg
1081 cggtgcgtat tacccacatc ccgaccggga tcgtgaccca gtgccagaac gaccgttccc
1141 agcacaagaa caaagatcag gccatgaagc agatgaaagc gaagctttat gaactggaga
1201 tgcagaagaa aaatgccgag aaacaggcga tggaagataa caaatccgac atcggctggg
1261 gcagccagat tcgttcttat gtccttgatg actcccgcat taaagatctg cgcaccgggg
1321 tagaaacccg caacacgcag gccgtgctgg acggcagcct ggatcaattt atcgaagcaa
1381 gtttgaaagc agggttatga ggaaccaata tgtctgaaca acacgaaaaa aaaaaaaaaa
1441 a
//