GenBank-Updates@genbank.bio.net (11/15/90)
LOCUS MUSLIFA 81 bp ss-mRNA ROD 15-NOV-1990
DEFINITION Mouse leukemia inhibitory factor (LIF) mRNA, 3' end.
ACCESSION M57691
KEYWORDS leukemia inhibitory factor.
SOURCE Mouse Krebs ascites tumor cell line, cDNA to mRNA, clone pLIFNK1.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 81)
AUTHORS Gough,N.M., Hilton,D.J., Gearing,D.P., Willson,T.A., King,J.A.,
Nicola,N.A. and Metcalf,D.
TITLE Biochemical charactization of murine leukaemia inhibitory factor
produced by Krebs ascites and by yeast cells
JOURNAL Blood Cells 14, 431-442 (1988)
STANDARD simple staff_entry
FEATURES from to/span description
pept < 1 81 leukemia inhibitory factor
BASE COUNT 21 a 17 c 24 g 19 t
ORIGIN
1 gccttccaaa ggaaaaagtt gggttgccag cttctgggga catacaagca agtcataagt
61 gtggtggtcc aggccttcta g
//GenBank-Updates@genbank.bio.net (04/11/91)
LOCUS MUSALIFA 8757 bp ds-DNA ROD 11-APR-1991
DEFINITION Mouse leukemia inhibitory factor (LIF) gene, complete cds.
ACCESSION M63419 J05435
KEYWORDS glycoprotein; leukemia inhibitory factor.
SOURCE Mouse DNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 8757)
AUTHORS Stahl,J., Gearing,D.P., Willson,T.A., Brown,M.A., King,J.A. and
Gough,N.M.
TITLE Structural organization of the genes for murine and human leukemia
inhibitory factor: Evolutionary conservation of coding and
non-coding regions
JOURNAL J. Biol. Chem. 265, 8833-8841 (1990)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
mRNA join(791..980,2541..2722,3280..>8757)
/gene="LIF"
/partial
mRNA join((800.801)..980,2541..2722,3280..>8757)
/gene="LIF"
/partial
mRNA join((898.899)..980,2541..2722,3280..>8757)
/gene="LIF"
/partial
mRNA join(902..980,2541..2722,3280..>8757)
/gene="LIF"
/partial
CDS join(962..980,2541..2722,3280..3690)
/product="leukemia inhibitory factor"
/gene="LIF"
/codon_start=962
sig_peptide join(962..980,2541..2563)
/gene="LIF"
/codon_start=962
mat_peptide join(2564..2722,3280..3687)
/product="leukemia inhibitory factor"
/gene="LIF"
/codon_start=2564
exon 791..980
/number=1
/gene="LIF"
exon (800.801)..980
/number=1
/gene="LIF"
exon (898.899)..980
/number=1
/gene="LIF"
exon 902..980
/number=1
/gene="LIF"
exon 2541..2722
/number=2
/gene="LIF"
exon 3280..>8757
/number=3
/gene="LIF"
BASE COUNT 1906 a 2360 c 2397 g 2072 t 22 others
ORIGIN
1 gagtcacctt tgtactctga ggccaagcac taggggcttc caaagagtgt cagccattcc
61 taggcaccca gaggaagcag gagccccatt ttgcccagcc tccctgatca ttgctttagg
121 ggtcccttca acgtagtaag gctgagttct tgcccagata gttcccagat agacgataca
181 acctcatact cttaccagtc ctccatagag ggggcatgct gatggctggg ggtgggcaag
241 accctctggc acactggcag caagcccctt tgtgtcttct ctttacccat tggcaatgga
301 caggctggag aaaggccacc cctaaggtga ctcccagcgt ccacattctg cttcacttta
361 tggctgtcta aagttcaaaa gccccggccc cccttctgag aaactgcttc tcagtggaac
421 cccagcctga ctccttttgc tgccccgcaa agccccacca ccaccagggg acacaaaggt
481 aacctccagt ctgttgccac cctcccaaac ccagttgaaa ctggaacgtc tgaagggggg
541 cacaggctga ggacccctct gaatccccct gagacccttt ccccaccaga cccatctgca
601 aaaaacgccc agagcaaacc acttaggaaa accacagggc ggttttttgt tgttgttgaa
661 gacttcatta taattttatc aatcaaattc ttagaagaag gaaaaagtct gccctcccca
721 ccctcccccc tcactcttcc cccctccccc ttcactctca ctttcttcca ttcataattt
781 cctatgatgc acctcaaaca acttcctgga ctggggatcc cggctaaata tagctgtttc
841 tctctgtctt acaacacagg ctccagtata taaatcaggc aaattcccca tttgagcatg
901 aacttctgaa aacggcctgc atctaaggtc tcctccaagg ccctctagag tccagcccat
961 aatgaaggtc ttggccgcag gtaaatccat gcgccgggcc gcgatttaag agtcccggct
1021 gcgggcgcgc cggcagcctg gggcgctggg cgagcacccg cgggacaccc gccccccgcg
1081 ccactcggac acttggggcg cccgcgcagt ccgggcccgc cggaggcgct gggtggcggc
1141 cgggagcgag cgccgcacat ggtccgggca ccgcgcgccc agcccgccgg ggccagcagg
1201 ttggcggtga gggacctggc ccggcgtagc cttgctccac cgccttctct cctcgcccca
1261 ctgctctcct gtgctgctct tacttctccc gtgtcgccta gatttaccct ccctctttct
1321 ttttctttct ttccgctttc tcttttccaa ctgcgtcccg ggctgctccc tgggaggggc
1381 gcaggcggct gagcagcttg caaactccgg ccaggaccgg cgcaggtgcg gcttccgtct
1441 gctagtccct ggaaagctgt gattggcgcg agatgagatg caggtatggg tacccccgca
1501 gctattccgg ggccctaaca gtgtttggga tgctgagatc gatagagact ccaggggagt
1561 aaacttaagg ttctagggtc tttttagtcc caggatgggg agttcaggtg ggaggtctcc
1621 agcctccctt aagagatgac ttcagcctct atcaccttct ggtgtcttta gcctggtgtt
1681 ggaaaggctg gagctggggt gagggggccg gtgaagctaa aagtgaagga cgggcaggcg
1741 ccccggggga ggggtgatcc tcgacaggcc cggacagctc ccggctctcc tgacaccttt
1801 cgctttcctc ttgcgtgtcc gcctgcgacc tttccccacc ccggcctctt tcctggttgc
1861 accacttcct ctcattccaa agtatgtgga tttttttggg cggggggggg gctgtagcaa
1921 gcaggggtga aagatgattt ggttggagag tgggcacctg cagaacaaac aggtggaaag
1981 ttctctatag ggcctcattg aggaagatgg gattaaaggc aatcgtttta ggaagccaga
2041 gtctagtggc agttttaaga gatggtctac agaaacccca gctcccagcc tgcacggcct
2101 gcaaggggtt cagctcagag ttctaggctc cagtgggcca atggacacca accaagttaa
2161 gaatctggcc ggtcctggaa aggggaggac ggtttatttc tcttcccctc tcctggacct
2221 aataccatcc atctagggcc aggaggatga ggctagatgg ttgagtgccc ccccccccag
2281 acctagcttt cagttggtac aaatggctcc caccttgata ccttgcctct taatccagtg
2341 gcatagatgg ggaaactgag gcctgggagc agggaaagag aaagccagag agccagggaa
2401 cctcagtaga gcaggagact aaaggctgat ggagggtggg agggggcagg aagggcctcg
2461 ctgaggtgcc cccccaaccc ccgtgccgtg ccctcagcca ggcttcttgc ctttccagtc
2521 accctccgtg tctcttccag ggattgtgcc cttactgctg ctggttctgc actggaaaca
2581 cggggcaggg agccctcttc ccatcacccc tgtaaatgcc acctgtgcca tacgccaccc
2641 atgccacggc aacctcatga accagatcaa gaatcaactg gcacagctca atggcagcgc
2701 caatgctctc ttcatttcct atgtaagtta ctttcctggg gtactgagga aggggggggc
2761 tgcctggccc ggaggggtgc ccttcagagc tggaagagcg ctgtgggaac ccatggctcc
2821 ctccccacac cctagccaaa gcacagagac tggtgggcac cactcgccag caagttgggg
2881 tgagcggcgg ggactgtgct ttctgtcttg tcccatggct cagggtacca aagaagaggc
2941 tatgcagtga atggacaggg aggtgtcatt gaaagcagtg tgtgtggggg gcccaggaag
3001 aggctggggt gactgaagtg caagtgtatg tggtgttctg gctgaggtga cacctgcgac
3061 atgccacatt tcctctatcc atttatgtca ccgtgacctt ggtgagtgag ttcacatttc
3121 tgatcattgc tgactaatga ttctagttgc ctacagggca gcaagtggag tccccatgtc
3181 acaggtgggg aaacagaagt gcaagagctt gccccaaggg ttggtggcgg gctagaacac
3241 tcaccctgac tcccacatca cctctcctct cttctgcagt acacagctca aggngagccg
3301 tttcccaaca acgtggaaaa gctatgtgcg cctaacatga cagacttccc atctttccat
3361 ggcaacggga cagagaagac caagttggtg gagctgtatc ggatggtcgc atacctgagc
3421 gcctccctga ccaatatcac ccgggaccag aaggtcctga accccactgc cgtgagcctc
3481 caggtcaagc tcaatgctac tatagacgtc atgaggggcc tcctcagcaa tgtgctttgc
3541 cgtctgtgca acaagtaccg tgtgggccac gtggatgtgc cacctgtccc cgaccactct
3601 gacaaagaag ccttccaaag gaaaaagttg ggttgccagc ttctggggac atacaagcaa
3661 gtcataagtg tggtggtcca ggccttctag agaggaggtc ttgaatgtac catggactga
3721 gggacctcag gagcaggatc cggaggtggg gagggggctc aaaatgtgct ggggtttggg
3781 acattgttaa atgcaaaacg gggctgctgg cagaccccag ggatttccag gtactcactg
3841 cactctgggc tgggccatga tggaatctgg caaagttgaa acttccatag gcagagcttc
3901 tatacagccc agcaccagct agaaatggca atgagggtgt tggtctgaga gatttctgtc
3961 tcactcactc actcactcac tctcactcac tcactcactc actcactcag ccccttgctt
4021 gctgggtgta gaacaagctg ccacaagttg tctacagcag acagcaaagg gctgggaagt
4081 gtcctagacc cctacagagt caccatcatc tggtcctttg ctgtctctca gagaaacttt
4141 ggaaggcttg gttgggatgt gagagagcta aggggactgg gatccagaag gaatcctttt
4201 attttatttt attttatttt attttatttt attttataag ttttgtgggt ggaagggtac
4261 cctggggtgg aatgatggaa tgtgtcttct cttgagttgg atgagagagt tcaggcttag
4321 agactgtcag atggaagagt ctacctcacc agtgttcagc tcccacagaa gcacagcggc
4381 cagcttccag ttgtcaaagc ctgacgaact cggttagctt ctatgcagtt ccccccacag
4441 cctggcgtgg ttggggtctg ccagctggac ctagaggtga ggtgtgtgca ggcaggaaga
4501 ggcaggctgc aaaggcaggt tcccagagtc ctcccgggga aggacctcta actgtctagg
4561 agtcagggaa ggagcaaggc agccagccat tgctgaggca gtagccgact gcagctctca
4621 tctgcttctc aacccctgag aacaggtgat cttgagcaga cagacaggta gcataaagta
4681 gaatgtcggg tctgaggccc cggaggtcgc aaaggtactt gaaggggacc agagggctgt
4741 cttgggtccc tggagcatgg agaagcagaa cttgaggtca gggtctcagg gaagatgagg
4801 cccagagtgc tgtgtttgat ccagcacagc tgtctattta ttactatgtc ctatttatat
4861 taacttattg gtgctttaaa tggcaaagtt aattccccga aatggtatga ggctccttcc
4921 atgggagctg gggccgagac tctccaccta gtggggcctg gtctggaggc acatgattgt
4981 tacaggtgca gctcatgggt caaatcagag agctggctag ctcctctgtc tcccactgtg
5041 actcactttt agggtgtcag ggtcccccag aaaaagctgg gccagtttgt ctctctgctt
5101 ctgtctctgt ctctccgagt ctgtctctgt ctgtctctgt gtctctgtct ctttgtctct
5161 ctctgtctct gtctctctct ctctctcccc gccccccccc cttctccctc tggtctccaa
5221 gggggtggaa cagtttcttg ttgttttgtc ccactgagct ctctggcacc ccctagattc
5281 ctgctatgcg gtgcaccatt cataatgaag tgaatggctc tggaaccttg ggcaaaactg
5341 attccttcct caaatcgtag ctgaggagtg ctgaaacatc ctgacccggc acccagcgtg
5401 ctttcgacca gcatggaagc tcctcgggtg gcccgaacac ccacagaggg tgaatacagg
5461 aggttggagc agtgcaggcc ctgaactggg cctgaacagc tgcccagtgc gccagagaag
5521 gggagatcaa ggcccgagac gcctgggaca cagaccagga agctgtggtc cttgcttcat
5581 cgctgccttc ccactcccgc ccatgtctgg gctcccaggc agggaatccg atctgatctc
5641 tcctttgtgc tgaggccagg caagcagagg aacgccctcg atctgggagc agggtaggga
5701 ggaaggcagc caagctgggg cagtggctga ctacagagct agctgcctgc ctctcaggct
5761 ctgaacaggg cggtccttag cagttcagca gtgggattct gcttcacgcg gttttgcacc
5821 tttctctgtc actctctaag cactttacct ggacggcagg tggacaggcc ctggagctct
5881 ggcttaggaa aggcctggaa ccatagatgc agcaaggaga ctatggtggg ggccacgcgt
5941 gtcagcgaca aagttactcc accgtactcc tgttgctgcg tcaggctcat ctcaggactg
6001 gctgcccttc tccaagctga gagtcaattt gtctaaaagc caagatgatg ccacagcctg
6061 gggcctgttg ggctttgtca tcacttcaca tttgtatgga cttggactct ctgctccgcc
6121 cacctggcag ctttgaaggc tcagggacca atggactctc tccgtgcacg cccccgtccc
6181 cccaacgcaa ccacctacct gcgtcttact ccatcagttg cccagcatcc cagaacctta
6241 gagcctttgg ggaaaacaga ctttaggggc aggtagttgc tcacctgaca tctttcacct
6301 ggaagcattg acttccaccg agcatagtag gtagtgtgtc tggaccagag aaaaagggat
6361 ggggcatttt gcagtttatc cagagagaag caaaggggcc tttatttatt atttaaaact
6421 tcaaacctga aagcactgag agtttactgg tctgcccccc tccccccact cttgtctatt
6481 tctgtgtcct tgatccccga ctcaagcaac ccagctctgc tttgcctgct ctctggagca
6541 gacatggtat gtgggccagg accccggagt cttgcatggt agcggcttca gaagggaaat
6601 gatatggctg tctgcattcg gatgactccc cagtcccagc ccagcctctc ctttgcactg
6661 ctgctctccc tctttccttt cctttggaag ggacttggcc ttgggtgaca aattcctctt
6721 tgatgaatgt accctgtggg aatgtttcat actgacagat tatttttatt tattcaatgt
6781 catatttaaa atatttattt tttatactga aggagtgtct ttttttttta aagaaaaaat
6841 gaaataataa agaactcatt cttgttgagc cttctggagt gcattgattt ggtgagggct
6901 tgtgggggaa cctcctgttg ggactatagg aggattggaa gatgagattt cctccatcgg
6961 tccaggaggg gtaggagggt ggtatttggg cccagaataa cagtgagtgg tgtttagaat
7021 atgaggtgcc actaagagag gggaccgatg gcttccatgg gtactggtgt gacatctcac
7081 taagcacaac agccagtttg ttcccggtta gctgaggaga gaactgaagc ccagagcgag
7141 gaactgaata tccagagtaa tctaaaatgt cgaggtcacc cctcccagtg agaggtgacc
7201 ccagctagtc cccagccaat gggtgtgggc ccactcagct cccaaccctc atgttctctc
7261 tgcgtggagt ttcagggatt tggaaatggc gtctgtctgg agctccgtca cccaggaagg
7321 gtccacaggc acaggagcag catcaacatt ggtccagagg ctcctatcag aatcacacga
7381 gacattgtgc cctaagtggg ctaactaagc agccatgaca gcatttctgc aaggcttttt
7441 tggggggggt accgatggct ctcagggagt gcttcctccc annnagagat gccttctcca
7501 cagcnccctt tctctacctt ggggaacaat ggggtcaaac tgttagacac aatgtgtctg
7561 gtaggtcccc taaatatctc ctgtactttg ctctggggtt tgaatcttgg gctccccctg
7621 agagattcag ttactggaaa gcannnnnnn nnnnnnnnaa gctttttagg cattggtaag
7681 tccaaggcag gaggttacag aatcatnngg caggcatctt cagccagcat ctcatcttct
7741 ctctgccagg ataggactga ctgcctccca cttctccagc tctgcttagt atgggcttcg
7801 cagggagctg gtgaggaatg tggagtccgt ggagtccagt gtcttgcctc gatgtatgag
7861 aggctgcagc ttaaaaacca gccagactcc tgggcatctg tggatatgtg gagtgagagg
7921 aaggctaccc tcaccagccc ctcatgacat cacccactaa cccagcaaag tggcctggtg
7981 gcgaccaaca tcgggaaagg ttctgaacat ccccttgctg gaagcccacg tgtcctttcc
8041 tcctgcgggc agatgagtgc tgccccgccc aaacaggccc ttatccatcc ttaaacctta
8101 tctacagttc agggaagagc agattgaaga tgcttagaag cacagtcagg agctgcaggg
8161 gtgtggacct aaggcagcct ctggggttgg tttctcaccc agggctttgg ttcttgggtt
8221 gaatccacca cctgcctgcc ctctgggaga ggccctggcc tggcctgatg gaacaatgga
8281 ctattaaagg ccattaggtc acactgagag tactgtgaca atcactgtgt gataggaaga
8341 agcggtggtg ggcctgctgt gctggaaagg ttggctgggg tccttgcctc tctccatcac
8401 cgtttatctc tcaatctatg aacctgtgag agcaatcaga gaggagagcg gccctcttta
8461 cttaaagtgg tgcccgaggg agctaaggga cagaaagaca gagatgtcat gtgaattgca
8521 tatctcgcct ggcctagctt gtttagccaa ggatggcagg acaggaagca gggtgaatgt
8581 cacagcccag ctgcctgctt tgctgtgaga gtctcatggc tccctgatgg ttgtggaagg
8641 gaaggttctg ctaactctga gtccaaagga tgccaggaca atctggaaga aggtcaacca
8701 atatcccaac aattgcttcc ctgatggtaa ctgagtccca ggtcccagga ccggatc
//