GenBank-Updates@genbank.bio.net (04/11/91)
LOCUS BACADDAA 8732 bp ds-DNA BCT 11-APR-1991
DEFINITION B.subtilis addA, addB and open reading frame 3, complete cds.
ACCESSION M63489
KEYWORDS ATP-dependent nuclease.
SOURCE B.subtilis DNA.
ORGANISM Bacillus subtilis
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 8732)
AUTHORS Kooistra,J. and Venema,G.
TITLE Cloning, sequencing, and expression of bacillus subtillis genes
involved in ATP-dependent nuclease synthesis
JOURNAL Unpublished (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 502..3999
/product="ATP-dependent nuclease"
/gene="addA"
/codon_start=502
/partial
CDS 3988..7684
/product="ATP-dependent nuclease"
/gene="addB"
/codon_start=3989
/partial
CDS 7759..8517
/note="open reading frame 3"
/codon_start=7759
/partial
BASE COUNT 2387 a 1877 c 2406 g 2062 t
ORIGIN
1 gtcgacggtt tcttgttcat ctttaaaagc ggcgtccagt cctcttgtga aattgtccgg
61 gcattcttcc gtttgtttcg gcatgatgtg tttgccggta attgaaccat acagtgtgga
121 aaattcacgg aggtggcgaa tctcgtcacg cctgatttct tcaatctgtt ttctgacctc
181 atcgctgtgg gcgagttcgg caagcttgcg gtagcattga atagcactga attctccgtt
241 gatggccttc tgcaaattcc gaatcaaaac aggatcttcc cggtatggca ccggataggg
301 atagtagctg taatgcaagg ggattcaact ccattcttag gaattgggct tctgtaaaag
361 tatatgttgg gcagggagag atgtgcggag ataatcagct ttttatatgt gaaaaggccg
421 tttttaccaa tagatcagat tggtcatttt cgtcaacatt cgataaaata tagagagata
481 aaaagagagg ggtcttctaa tttgggagca gagtttttag taggcaggtc cgggagtgga
541 aaaacgaagc tgatcatcaa cagcattcag gatgaattgc gccgggctcc attcgggaag
601 ccgatcattt ttctagtccc ggatcaaatg acgtttttaa tggaatacga gcttgctaaa
661 acgccagata tgggcgggat gatacgcgct caagtgttca gtttttcacg attggcctgg
721 cgcgtcctcc agcatacggg aggaatgagc aggccgtttc tgacgagcac cggcgtacaa
781 atgctcctgc ggaagctcat tgaggagcat aaacaggagt tcaaagtcta tcaaaaagcg
841 agtgacaaaa gcgggtttac cgcacaggtt gagcggatgc tgacagagtt taagcgctat
901 tgtctggaac cggaggatat ccgccggatg gcggaaagcg gaacggcttc cgagtatcgc
961 ggagaacgtg ttttatctga aaagctccat gacttatcaa ttctgtatca gcaaatggaa
1021 aaaagcctcg cagatcaata tcttcactct gaggattatt tgacattgct ggcagagcat
1081 atcccgcttg cggaagatat aaaaggcgcc catatctatg tggatggctt ttatcagttt
1141 accccgcagg aattcagggt gttggagcag cttatggttc atgcggagca tatcacgttt
1201 tcgctcacag cggacaagcc gtcatatgag cgggagccgc atgaactgga attgttcaga
1261 atgacgggga aaacctatta ccgcctgcat cagaaggcga aggaactgaa tttggacatt
1321 acgtataaag agctgagcgg gactgagcgg catacaaaga cgccggaatt ggcgcatcta
1381 gaggcacagt atgaagcgcg tccggccatt ccatacgcag aaaaacaaga agcccttact
1441 gtgatgcagg ccgcaaacag acgagctgag ctggaaggca ttgctcggga aattcacgcc
1501 ctagtcagag agaagggata tcgctataag gatgtagcaa ttcttgcgcg ccagccggaa
1561 gactacaagg atatggtgaa ggaagttttc gcagattacg agattcctta tttcattgac
1621 ggaaaagcat ctatgctgaa ccatccgtta attgaattta tccggtcgag ccttgatgtt
1681 ctgaaaggga attggcgtta tgaagcggtg tttcgctgcg tgaaaaccga actgctattc
1741 ccgctcaatg aaccgaaggc caaagtgaga gaacaggtcg atcagctcga aaattactgt
1801 atcgcctatg gtattaaagg cgaccgctgg acaaagggcg atcggttcca atacaggcgt
1861 tttgtgtcat tggatgatga ttttgcgcag actgatcagg aaatcgaaat ggaaaacatg
1921 ttgaatgaca cccgcgattg gattgttccg ccgctttttc agcttcaaaa acgcatgaaa
1981 aaagcgaaga cggttcaaga gaaggcggag gcgctctatc gttatttaga agagacggat
2041 gtgccgctga agctggatca ggaaaggcag cgtgctgagg atgacggcag aatcattgaa
2101 gcgcagcagc atcagcaggc gtgggacgca gtcattcagc tgcttgagga gtttgttgaa
2161 atgatgggag atgatgagat ttctcttgat ttgtttcagc aaatgataga agccggcgcg
2221 gagtcgctta cattttctct aattccgcct gcgcttgacc aggtgtttgt cggcaatatg
2281 gatttgtcca gaatgtatgg cacctcctgc acctttgtgc tcggggcaaa cgacggcgtt
2341 ctgccggcac gccctgatga aaacggggtc ctgtcggatg atgaccggga atggctgaaa
2401 acgattgggg ttgagctatc ctcaggcgga cgagagcgtt tgcttgatga gcacttcctc
2461 atctacatgg cgttttcaag tccgtctgac cggctttacg tatcgtatcc gattgctgat
2521 gcggaaggaa aaacgctttt gccgtcgatg atcgttaagc ggctggaaga actgtttccg
2581 catcataagg agcgcctgtt aacaaatgaa cctgaacagg tcagcgatga ggaacagctg
2641 atgtatgttg tgaataaaag cgtggcacag tcctttaccg cgagccagct caggttatgg
2701 actcgggaat acgacatcag cgacgtctgg tggagcacgt acaatgtgct gatgagtgag
2761 caggacaggc tgcaatcgaa aaagctgttc tcaagcctgt ttttccggaa tgaagtgaag
2821 cagcttgaac gcagcgtgtc gagacagctc tatggtgaac gtattcaggg cagtgtatcg
2881 agaatggaaa cctttaacgc atgcccgttt tcccattttg cgtcacacgg gctgcatttg
2941 aaggaacggc aattcttcaa gcttgaagca ccggatatcg gccagctgtt tcattccagc
3001 ttaaagctga tttcggacag gctgcgtgac gaaaagctgg attggcgcga tttaacgaag
3061 gagcagtgcg agctgttttc ctatgatgcg gtagagcggc tggcaccgaa actgcaaaag
3121 gaaattctgc tcagctcaaa ccggcattat tatgtgaagg aaaaactgca aaaaattgtg
3181 acacgtgtgt ccggcatttt aagcgagcat gcgaaagcga gcggattcgt accgatcggg
3241 cttgaactgg gctttggagg aaaagggccg cttccgccgc tgacctttca actgaaaaac
3301 ggctgtacga tggaactcgt cgggcgaatt gaccgtgttg ataaggctga aagctcaaaa
3361 ggcctgctcc tcaggattgt cgattataaa tcaagcgaca aaggccttga cttagcggaa
3421 gtatattacg gattggcact gcaaatgctg acgtaccttg atttatcgat tacacattca
3481 gctgactggc tcgggatgag ggcgacgcct gccggagtgc tgtatttcca tattcatgac
3541 ccgatgattc aatctaatct cccgcttggg cttgacgaga ttgaacagga gatctttaag
3601 aaatttaaaa tgaagggctt gctcctcggt gatcaggaag tggttcgcct catggataca
3661 acccttcaag agggacgttc aaatatcata aacgccggct tgaaaaaaga cggctctctc
3721 agatcagact cagcagcagt cggtgaaaag gaatttgatc ttttgacaaa gcatgtgcgc
3781 cgcaccttcc aagaagcggg cgaacaaatc accgacgggc gcgtatccat tgagccgtac
3841 aaaatgaaga acaagacgcc gtgcacatac tgtgcgttca aatcagtatg ccaatttgat
3901 gaatcactgg aagaaaacga gtatcgccca ttaaaggctg aaaaggacaa gacaatactt
3961 gagtggataa aaaaggaggc ggatggcaat gaacattcct aaaccggcag acagcacatg
4021 gacagatgac caatggaatg ccattgtttc aaccggccag gatattcttg tggcagcggc
4081 tgcgggctct ggtaaaaccg ctgtgctcgt tgaacgaatg attcggaaaa tcaccgcgga
4141 ggaaaaccca atagatgtag accgtcttct cgttgtgaca tttacaaacg cctcagcggc
4201 agagatgaag caccgaatcg cagaagcctt ggaaaaagag cttgtacagc gccccggctc
4261 gctgcatatt agacgccagc tgtctctttt aaaccgggcc agcatttcga cgctccattc
4321 cttttgcctg caagtgctga agaaatatta ctacttgatc gatcttgatc cgggctttcg
4381 gatagctgat cagacggaag gcgagctgat cggggatgaa gtgcttgacg agctgtttga
4441 agacgaatac gcaaagggcg aaaaggcgtt ttttgagctt gttgaccgct atacgacaga
4501 ccggcatgat ctggatctgc aatttctcgt taaacaggtg tacgagtatt cccgatccca
4561 tcccaacccg gaggcgtggc tggaaagctt tgttcatttg tatgatgtat cagaaaagag
4621 cgccatcgag gagctgccgt tttatcaata tgtcaaagaa gatattgcaa tggtgcttaa
4681 cggggcgaag gaaaagctct tgcgcgcgct tgagctgacg aaagcgccgg gcggcccggc
4741 gccgcgtgct gacaattttc ttgatgatct tgctcagatt gatgaactga ttcagcatca
4801 ggacgatttc agtgaactat ataagcgggt gcccgccgtc tcttttaagc gtgccaaagc
4861 agtaaaaggg gatgagttcg atccagcgct ccttgatgag gcgacagatt tgaggaacgg
4921 cgcaaaaaaa ctgcttgaaa agctcaaaac cgactacttc acgcgaagtc ctgaacagca
4981 cttgaaaagc ctagccgaga tgaagcctgt cattgaaacg ctcgtacagc ttgtcatcag
5041 ctatggaaaa cgattcgaag ctgcgaaaca ggaaaaatca atcatcgatt tttcggattt
5101 ggagcattac tgtttagcga ttttgacagc tgagaatgac aaaggtgaac gtgagccgag
5161 cgaggctgca aggttttatc aggaacagtt tcatgaggtg ctcgttgacg aatatcagga
5221 taccaacctc gtgcaggaat cgattctgca gctcgtcaca agcggtccgg aggagactgg
5281 taacctgttt atggtaggag atgtcaaaca gtcgatttat cgattcaggc ttgcggagcc
5341 gcttcttttc ctctctaaat acaaacggtt tacagagagc ggagaaggca cggggcggaa
5401 aatcgattta aataaaaatt tccgaagccg ggctgatatt ttagacagca caaacttttt
5461 atttaaacag ctgatgggcg gcaaaatcgg tgaggtcgat tatgacgagc aggctgagct
5521 gaagcttggt gcagcgtatc cggacaatga cgagacggaa acagagctgc tgctgatcga
5581 caacgcagaa gatacggatg caagcgagga agcagaagag cttgaaacgg tgcagtttga
5641 ggcaaaagcc atcgctaagg aaattcgtaa gctgatttca tcgccgttta aggtgtatga
5701 cggaaaaaag aaaacacatc gcaatattca ataccgagat atcgtgattt tgctccgttc
5761 gatgccgtgg gctccgcaaa tcatggagga gctgagagca cagggcatac cggtttacgc
5821 caatttaacg tcaggctatt ttgaagcggt cgaagtcgcc gtcgcgcttt ctgtgctgaa
5881 ggtgattgat aatccgtatc aggatatacc gcttgcctct gtgctgcgct caccgattgt
5941 cggagcagat gaaaacgagc tgtctttgat ccggcttgaa aataaaaaag cgccgtacta
6001 tgaggcgatg aaagactacc tggctgctgg tgaccggagc gatgagcttt atcaaaagtt
6061 aaatacgttt tacggacatc tgcaaaaatg gcgcgcgttt tcgaaaaacc actcagtatc
6121 tgagctgatt tgggaagtgt accgcgacac caaatatatg gattatgtcg gcggcatgcc
6181 gggcggaaaa cagcgccagg ccaatttgcg tgttctttat gacagggcgc gtcaatatga
6241 atcaacggca tttcgcggct tgttccgttt cctgcggttt atcgaacgca tgcaggagcg
6301 gggcgatgat cttggtacgg cgagaggcct cagcgagcag gaggatgttg tccgcttaat
6361 gacgatccac agcagcaaag ggctcgaatt tccagtcgtg tttgtagcag gtctcggccg
6421 gaatttcaac atgatggatt tgaacaaatc gtacctgctg gataaggagc tcggatttgg
6481 cacgaagtat attcatccgc aattacgcat cagctatccg acacttccgc tcattgcgat
6541 gaagaaaaaa atgcgcaggg agctgctgtc agaggaattg cgtgtgctct atgttgcatt
6601 aacgagagcg aaggaaaagc tgtttctgat tggctcatgt aaggatcatc agaaacagct
6661 tgcaaaatgg caggcatccg cgtcccaaac tgattggctt ctgccggaat ttgaccgcta
6721 tcaggcgaga acgtatctag atttcattgg gccggctctt gccaggcaca gagacttggg
6781 ggatttggct ggtgtgccag cacacgctga catctcaggt cacccggctc gttttgccgt
6841 tcaaatgatc cattcctatg atttgcttga tgatgatctg gaagaaagaa tggaagaaaa
6901 aagcgagcgc ctagaagcga tccgccgagg tgaaccagtt cccggctcgt ttgcgtttga
6961 tgaaaaagcc cgcgagcagc tgagctggac ctacccgcat caagaagtga cgcaaattcg
7021 gacaaagcaa tcagtttctg agatcaagag aaaaagagag tacgaggatg aatacagcgg
7081 cagggcccct gtaaaaccgg ctgatggaag cattctgtac agacgtcccg cttttatgat
7141 gaaaaaaggc ctgacagcgg cagagaaagg gactgccatg catacggtta tgcagcatat
7201 cccgctgtca catgtgccgt cgatagaaga agctgagcag acggttcaca ggctttatga
7261 aaaagagctt ctcactgaag aacaaaaaga cgctattgat atagaagaaa tcgtgcaatt
7321 tttccataca gaaatcggcg gacagctgat cggtgctaag tggaaggacc gggaaatacc
7381 attcagctta gcgcttccgg ccaaggagat ctatcctgat gcacacgagg cagatgagcc
7441 gcttttagtg cagggtatta ttgactgtct ctatgaaact gaggacggat tatatctatt
7501 ggattataag tcggaccgga ttgagggcaa attccagcat ggatttgaag gagcggcccc
7561 gatcttgaag aaacgatatg aaacgcaaat tcagctgtac acgaaggcag tggagcaaat
7621 tgcaaaaaca aaggtaaagg gatgtgcgct ttatttcttt gacggagggc acattctgac
7681 attatagcga gatccataag ctccggaatt tcaggcggag cgggtctcgt tttctatata
7741 aaagagaggt gaaagcattt gcggatttta catacggctg actggcatct tggaaaaacg
7801 cttgaaggaa gaagcaggct gagtgaacag gccgatgtgc tggatgaact gaatacgatt
7861 gtaaaggatg agcagatcga tgccattgta atggcggggg atgcatttga caccgtaaac
7921 ccgccagctt tagccgagca gctgttttat gaaagcctgt ctgcgcttag cgacagagga
7981 aagcgcccga tcgtcgtcat tgccggaaat cacgataatc ctgaccgttt gtccgccgct
8041 tcaccgctga cacatgaaaa cggcattcat ttaatcggtt atccgacaac agagccgatt
8101 catattgaag tgccttcagc aggagagctt ttggcggttg gagcgctggc gtacccatct
8161 gaagcgcggt taaatgaagt gctatccgat acgtttgatg aaaagctgct ccgtgatcac
8221 tatgatgtga aaatcaggca ggcgtttgag catatgacaa gccggttccg gaccgatgca
8281 gtgaagattg ccgccagcca tatttatgtg gcaggcgaaa ccaaaccgat tcagacgggc
8341 cgattgaagt cggcggtgca tacacggtgg ctgctgaaag cctgccggca gatgccgctt
8401 acgttgcgct cggccatttg catcgcccgc aaacgatcaa gcgggcgcgg acgcttgcgc
8461 gttattcagg atctccgctc gcctacagct tttctgaagc gggctacgct aaatcagtga
8521 cgattgtgga cgcaaagcct ggggaagagg ccacttggca agaggtgtta ttatcaagcg
8581 gcaagccact tgtgaagtgg aaagcggcaa atggattaag tgaggtgtac agctggctgg
8641 atgaaggcag agatcagaat gcttggattg acttggaaat ccgtgtcgcg gaccagctgt
8701 cacttgaaga gattcacagg ctgcggaagg cg
//