GenBank-Updates@genbank.bio.net (04/11/91)
LOCUS BACNPRAA 1974 bp ds-DNA BCT 11-APR-1991
DEFINITION B.caldolyticus neutral protease (npr) gene, complete cds.
ACCESSION M63575
KEYWORDS neutral protease.
SOURCE B.caldolyticus DNA.
ORGANISM Bacillus caldolyticus
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 1974)
AUTHORS van den Burg,B., Enequist,H.G., Eijsink,V.G., Stulp,B.K. and
Venema,G.
TITLE A highly thermostable neutral protease from bacillus caldolyticus:
Cloning and expression of the gene in bacillus subtilis and
characterization of the gene product
JOURNAL Unpublished (1991)
STANDARD simple staff_entry
FEATURES Location/Qualifiers
CDS 318..1949
/product="neutral protease"
/gene="npr"
/codon_start=318
/partial
BASE COUNT 496 a 482 c 567 g 429 t
ORIGIN
1 cctgaatata atgccgacaa tccaaaagaa tataagttca gcggcaaacg tattaagcgc
61 ggcttgtatc gaacaaagtc tggcaaacta attaatgctg atgtcaatgg cgcattaaac
121 atcttaaaga aaagtaaagc tgtagacctg agtgtcttat gctctagcgg cgaagtggac
181 acgcctcaaa gaataaggat tgcttgaagc agtcaaactt ctttgggaag gccccacttc
241 aaattttccg ctagaaatta agtgggggta gttcactaga ataaataatt tgaaaatata
301 agaaaggggt aaagggaatg aacaaacggg cgatgcttgg cgcgattggg ttggcgtttg
361 gattgatggc ttggccgttc ggggcttcgg cgaagggaaa gtcaatggtg tggaacgaac
421 agtggaagac gccgtcgttc gtttctggtt cgctgttagg cagatgctcg caagaattgg
481 tttaccgtta tcttgatcaa gagaagaata cgttccagct tggtggacaa gcccgcgaac
541 gcctgagctt gattggcaac aaactggatg aactcggcca tacggtcatg cgttttgaac
601 aggctatcgc ggcatccctg tgtatgggcg ccgtgctcgt tgcccatgtc aacgatggcg
661 aattgtcctc tttgtcgggt acgctcattc cgaatttgga caaacgaacg ttgaaaacag
721 aagctgccat ttccattcaa caagcggaaa tgatcgcgaa acaagatgtg gctgataggg
781 tgacgaaaga acggccggcg gccgaagagg gaaaaccaac gcggcttgtc atctatccgg
841 atgaggagac cccgcgcctc gcctatgaag tcaatgtccg ctttttaacc cctgtcccag
901 gcaactggat ctacatgatc gatgccgcag acggaaaagt gttgaacaaa tggaaccaaa
961 tggacgaggc gaagccgggc ggcgcgcagc cggtcgccgg cacgtcgacg gtcggcgtgg
1021 gccgcggtgt gttgggggat cagaaatata tcaatacgac gtattcctcg tattatggct
1081 actactattt gcaagacaat acgcgcggca gcggcatttt tacgtatgac ggacgaaacc
1141 gcaccgtttt gcccggcagc ttgtgggccg atggcgacaa ccaatttttc gccagctatg
1201 acgcggcggc cgtggacgcc cattattacg ccggcgtcgt gtatgattac tacaaaaatg
1261 tgcacggccg gctgagctat gacggcagca acgccgccat ccgttcgacc gtccattatg
1321 gccgtggcta caacaacgcg ttttggaacg gttcgcaaat ggtgtacggc gatggcgacg
1381 gacagacgtt tttgccgttt tccggcggca ttgacgtcgt ggggcatgag ttgacccatg
1441 cggtgacgga ttatacggcc gggcttgttt accaaaacga atccggcgcc atcaatgaag
1501 cgatgtccga tattttcggc acgctcgtgg agttctacgc caaccgcaac ccggactggg
1561 agatcggcga agacatttac acgcctgggg tcgccggcga tgcgctccgc tcgatgtccg
1621 acccggcgaa atacggcgat ccggatcatt attccaaacg gtacaccggc acgcaagaca
1681 acggcggcgt ccatacgaac agcggcatca tcaataaagc ggcgtacttg ctcagccaag
1741 gcggcgtcca ttacggcgtg agcgtcaccg gcatcggccg cgacaaaatg gggaaaattt
1801 tctaccgggc gcttgtctac tatttgacgc cgacgtcgaa cttcagccag ctgcgcgccg
1861 cctgcgtgca agcggccgct gatttgtacg ggtcgacaag ccaagaagtc aactcggtga
1921 aacaggcgtt caatgcggtt ggagtgtatt aagacgaatg aagtttctga cgtc
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