GenBank-Updates@genbank.bio.net (03/16/91)
LOCUS ECOENTF 4756 bp ds-DNA BCT 16-MAR-1991 DEFINITION Escherichia coli enterobactin (entF) gene, complete cds. ACCESSION M60177 J05325 KEYWORDS entF gene; enterobactin. SOURCE E.coli DNA. ORGANISM Escherichia coli Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively anaerobic rods; Enterobacteriaceae. REFERENCE 1 (bases 1 to 4756) AUTHORS Rusnak,F., Sakaitani,M., Drueckhammer,D., Reichert,J. and Walsh,C.T. TITLE Biosynthesis of the E.coli siderophore enterobactin: Sequence of the entF gene, expression and purification of EntF, and analysis of covalent phosphopantetheine JOURNAL Biochemistry (1991) In press STANDARD full staff_entry FEATURES Location/Qualifiers CDS 393..4274 /product="enterobactin" /gene="entF" /codon_start=393 BASE COUNT 1035 a 1247 c 1356 g 1118 t ORIGIN 1 gatcgagccg atgatcatgc gggccaatca ggcgctgtat gccgaattac accccataaa 61 agaatccatt ttctggcgtc aggttgacgg cggacatgat gcgctttgtt ggcgcggtgg 121 cttgatgcag gggctaatcg acctctggca accacttttc catgacagga gttgaatatg 181 gcattcagta atcccttcga tgatccgcag ggagcgtttt acatattgcg caatgcgcag 241 gggcaattca gtctgtggcc gcaacaatgc gtcttaccgg caggctggga cattgtgtgt 301 cagccgcagt cacaggcgtc ctgccagcag tggctggaag cccactggcg tactctgaca 361 ccgacgaatt ttacccagtt gcaggaggca caatgagcca gcatttacct ttggtcgccg 421 cacagcccgg catctggatg gcagaaaaac tgtcagaatt accctccgcc tggagcgtgg 481 cgcattacgt tgagttaacc ggagaggttg attcgccatt actggcccgc gcggtggttg 541 ccggactagc gcaagcagat acgctgcgga tgcgttttac ggaagataac ggcgaagtct 601 ggcagtgggt cgatgatgcg ctgacgttcg aactgccaga aattatcgac ctacgaacca 661 acattgatcc gcacggtact gcgcaggcat taatgcaggc ggatttgcaa caagatctgc 721 gcgtcgatag cggtaaacca ctggtctttc atcagctgat acaggtggcg gataaccgct 781 ggtactggta tcagcgttat caccatttgc tggtcgatgg ctttagtttc ccggccatta 841 cccgccagat cgccaatatt tactgcacat ggctgcgtgg cgaaccaacg cctgcttcgc 901 catttacgcc tttcgctgat gtagtggaag agtaccagca ataccgcgaa agcgaagcct 961 ggcagcgtga tgcggcattc tgggcagaac agcgtcgtca actgccgccg cccgcgtcac 1021 tttctccggc acctttaccg gggcgcagcg cctcggcaga tattctgcgc ctgaaactgg 1081 aatttaccga cggggaattc cgccagctgg ctacgcaact ttcaggtgtg cagcgtaccg 1141 atttagccct tgcgctggca gccttgtggc tggggcgatt gtgcaatcgt atggactacg 1201 ccgccggatt tatctttatg cgtcgactgg gctcggcggc gctgacggct accggacccg 1261 tgctcaacgt tttgccgttg ggtattcaca ttgcggcgca agaaacgctg ccggaactgg 1321 caacccgact ggcagcacaa ctgaaaaaaa tgcgtcgtca tcaacgttac gatgccgaac 1381 aaattgtccg tgacagcggg cgagcggcag gtgatgaacc gctgtttggt ccggtactca 1441 atatcaaggt atttgattac caactggata ttcctgatgt tcaggcgcaa acccataccc 1501 tggcaaccgg tccggttaat gaccttgaac tggccctgtt cccggatgta cacggtgatt 1561 tgagtattga gatcctcgcc aataaacagc gttacgatga gccaacgtta atccagcatg 1621 ctgaacgcct gaaaatgctg attgcccagt tcgccgcgga tccggcgctg ttgtgcggcg 1681 atgtcgatat tatgctgcca ggtgagtatg cgcacgtggc gcagctcaac gccactcagg 1741 ttgagattcc agaaaccacg cttagcgcgc tggtggcaga acaagcggca aaaacaccgg 1801 atgctccggc gctggcagat gcgcgttacc tgttcagcta tcgggaaatg cgcgagcagg 1861 tggtggcgct ggcgaatctg ctgcgtgagc gcggcgttaa accaggggac agcgtggcgg 1921 tggcactacc gcgctcggtc tttttgaccc tggcactcca tgcgatagtt gaagctggag 1981 cggcctggct accgctggat accggctatc cggacgatcg cctgaaaatg atgctggaag 2041 atgcgcgtcc gtcgctgtta attaccaccg acgatcaact gccgcgcttt agcgatgttc 2101 ccaatttaac aagcctttgc tataacgccc cgcttacacc gcagggcagt gcgccgctgc 2161 aactttcaca accgcatcac acggcttata tcatctttac ctctggctcc accggcaggc 2221 cgaaaggggt aatggtcggg cagacggcta tcgtcaaccg cctgctttgg atgcaaaatc 2281 attatccgct tacaggcgaa gatgtcgttg cccaaaaaac gccgtgcagt tttgatgtct 2341 cggtgtggga gtttttctgg ccgtttatcg caggggcaaa actggtgatg gctgaaccgg 2401 aagcgcaccg cgacccgctc gctatgcagc aattctttgc cgaatatggc gtaacgacca 2461 cgcactttgt gccgtcgatg ctggcggcat ttgttgcctc gctgacgccg caaaccgctc 2521 gccagagttg cgcgacgttg aaacaggttt tctgtagtgg tgaggcctta ccggctgatt 2581 tatgccgcga atggcaacag ttaactggcg cgccgttgca taatctatat ggcccgacgg 2641 aagcggcggt agatgtcagc tggtatccgg cttttggcga ggaactggca caggtgcgcg 2701 gcagcagtgt gccgattggt tatccggtat ggaatacggg tctgcgtatt cttgatgcga 2761 tgatgcatcc ggtgccgccg ggtgtggcgg gtgatctcta tctcactggc attcaactgg 2821 cgcagggcta tctcggacgc cccgatctga ccgccagccg ctttattgcc gatccttttg 2881 ccccaggtga acggatgtac cgtaccggag acgttgcccg ctggctggat aacggcgcgg 2941 tggagtacct cgggcgcagt gatgatcagc taaaaattcg cgggcagcgt atcgaactgg 3001 gcgaaatcga tcgcgtgatg caggcgctgc cggatgtcga acaagccgtt acccacgcct 3061 gtgtgattaa ccaggcggct gccaccggtg gtgatgcgcg tcaattggtg ggctatctgg 3121 tgtcgcaatc gggcctgccg ttggatacca gcgcattgca ggcgcagctt cgtgaaacat 3181 tgccaccaca tatggtaccg gtggttctgc tgcaacttcc acagttacca cttagcgcca 3241 acggcaagct ggatcgcaaa gccttaccgt tgcctgaact gaaggcacaa gcgccagggc 3301 gtgcgccgaa agcgggcagt gaaacgatta tcgccgcggc attctcgtcg ttgctggggt 3361 gtgacgtgca ggatgccgat gctgatttct tcgcgcttgg cggtcattcg ctactggcaa 3421 tgaaactggc agcgcagtta agtcggcagg ttgcccgcca ggtgacgccg gggcaagtga 3481 tggtcgcgtc aactgtcgcc aaactggcaa cgattattga tgctgaagaa gacagcaccc 3541 ggcgtatggg attcgaaacc attctgccgt tgcgtgaagg taatggcccg acgctgtttt 3601 gtttccatcc tgcgtccggt tttgcctggc agttcagcgt gctctcgcgt tatctcgatc 3661 cacaatggtc gattatcggc attcagtcac cgcgccccaa tggccccatg cagacggcgg 3721 caaacctgga tgaagtctgc gaagcgcatc tggcaacgtt acttgaacaa caaccgcatg 3781 gcccttatta cctgctgggg tattcccttg gcggtacgct ggcgcagggt attgcggcgc 3841 gactgcgtgc ccgtggcgaa caggtggcat ttcttggctt gctggatacc tggccgccag 3901 aaacgcaaaa ctggcaggaa aaagaagcta atggtctgga cccggaagtg ctggcggaga 3961 ttaaccgcga acgcgaggcc ttcctggcag cacagcaggg aagtacttca acggagttgt 4021 ttaccaccat tgaaggcaac tacgctgatg ctgtgcgcct gctgacgact gctcatagcg 4081 taccgtttga cggtaaagcg acgctgtttg ttgctgaacg cacacttcag gaaggtatga 4141 gtcccgaacg cgcctggtcg ccgtggatag cggagctgga tatctatcgt caggattgtg 4201 cgcatgtgga tattatctct ccagggacgt ttgaaaaaat tgggccgatt attcgcgcaa 4261 cgctaaacag gtaaattaat attatttata aacccataat tacagaaaat aattatgggt 4321 tttttatttg tttgatttat aggtttgatg aatatttctc ttaaatagag tgaatgttgc 4381 attcagtgga aggaatttac aaacttcagc ttcggataat tatcaccaac tggtaaagtg 4441 agcgccgtgg agatttcccc ctattggact cattttcagg ttatacccca tgtcatcact 4501 gaatattaaa cagggaagtg acgctcattt tcccgattat cctctggcgt cgcccagtaa 4561 taatgaaatt gatttactta atctaatctc agttttatgg cgggccaaaa aaacggtcat 4621 ggcggtcgtt tttgcgtttg cctgcgcagg cttgctgatc tctttcatcc tgccgcaaaa 4681 atggaccagc gcggcggttg tcacgcctcc agaacctgtt cagtggcaag agttggagaa 4741 atcattcact aagctt //
GenBank-Updates@genbank.bio.net (04/12/91)
LOCUS ECOENTF 4756 bp ds-DNA BCT 12-APR-1991 DEFINITION Escherichia coli enterobactin (entF) gene, complete cds. ACCESSION M60177 J05325 KEYWORDS entF gene; enterobactin. SOURCE E.coli DNA. ORGANISM Escherichia coli Prokaryota; Bacteria; Gracilicutes; Scotobacteria; Facultatively anaerobic rods; Enterobacteriaceae. REFERENCE 1 (bases 1 to 4756) AUTHORS Rusnak,F., Sakaitani,M., Drueckhammer,D., Reichert,J. and Walsh,C.T. TITLE Biosynthesis of the Escherichia coli siderophore enterobactin: Sequence of the entF gene expression and purification of ENtF, and analysis of covalent phosphopantetheine JOURNAL Biochemistry 30, 2916-2927 (1991) STANDARD full staff_entry FEATURES Location/Qualifiers CDS 393..4274 /product="enterobactin" /gene="entF" /codon_start=393 BASE COUNT 1035 a 1247 c 1356 g 1118 t ORIGIN 1 gatcgagccg atgatcatgc gggccaatca ggcgctgtat gccgaattac accccataaa 61 agaatccatt ttctggcgtc aggttgacgg cggacatgat gcgctttgtt ggcgcggtgg 121 cttgatgcag gggctaatcg acctctggca accacttttc catgacagga gttgaatatg 181 gcattcagta atcccttcga tgatccgcag ggagcgtttt acatattgcg caatgcgcag 241 gggcaattca gtctgtggcc gcaacaatgc gtcttaccgg caggctggga cattgtgtgt 301 cagccgcagt cacaggcgtc ctgccagcag tggctggaag cccactggcg tactctgaca 361 ccgacgaatt ttacccagtt gcaggaggca caatgagcca gcatttacct ttggtcgccg 421 cacagcccgg catctggatg gcagaaaaac tgtcagaatt accctccgcc tggagcgtgg 481 cgcattacgt tgagttaacc ggagaggttg attcgccatt actggcccgc gcggtggttg 541 ccggactagc gcaagcagat acgctgcgga tgcgttttac ggaagataac ggcgaagtct 601 ggcagtgggt cgatgatgcg ctgacgttcg aactgccaga aattatcgac ctacgaacca 661 acattgatcc gcacggtact gcgcaggcat taatgcaggc ggatttgcaa caagatctgc 721 gcgtcgatag cggtaaacca ctggtctttc atcagctgat acaggtggcg gataaccgct 781 ggtactggta tcagcgttat caccatttgc tggtcgatgg ctttagtttc ccggccatta 841 cccgccagat cgccaatatt tactgcacat ggctgcgtgg cgaaccaacg cctgcttcgc 901 catttacgcc tttcgctgat gtagtggaag agtaccagca ataccgcgaa agcgaagcct 961 ggcagcgtga tgcggcattc tgggcagaac agcgtcgtca actgccgccg cccgcgtcac 1021 tttctccggc acctttaccg gggcgcagcg cctcggcaga tattctgcgc ctgaaactgg 1081 aatttaccga cggggaattc cgccagctgg ctacgcaact ttcaggtgtg cagcgtaccg 1141 atttagccct tgcgctggca gccttgtggc tggggcgatt gtgcaatcgt atggactacg 1201 ccgccggatt tatctttatg cgtcgactgg gctcggcggc gctgacggct accggacccg 1261 tgctcaacgt tttgccgttg ggtattcaca ttgcggcgca agaaacgctg ccggaactgg 1321 caacccgact ggcagcacaa ctgaaaaaaa tgcgtcgtca tcaacgttac gatgccgaac 1381 aaattgtccg tgacagcggg cgagcggcag gtgatgaacc gctgtttggt ccggtactca 1441 atatcaaggt atttgattac caactggata ttcctgatgt tcaggcgcaa acccataccc 1501 tggcaaccgg tccggttaat gaccttgaac tggccctgtt cccggatgta cacggtgatt 1561 tgagtattga gatcctcgcc aataaacagc gttacgatga gccaacgtta atccagcatg 1621 ctgaacgcct gaaaatgctg attgcccagt tcgccgcgga tccggcgctg ttgtgcggcg 1681 atgtcgatat tatgctgcca ggtgagtatg cgcacgtggc gcagctcaac gccactcagg 1741 ttgagattcc agaaaccacg cttagcgcgc tggtggcaga acaagcggca aaaacaccgg 1801 atgctccggc gctggcagat gcgcgttacc tgttcagcta tcgggaaatg cgcgagcagg 1861 tggtggcgct ggcgaatctg ctgcgtgagc gcggcgttaa accaggggac agcgtggcgg 1921 tggcactacc gcgctcggtc tttttgaccc tggcactcca tgcgatagtt gaagctggag 1981 cggcctggct accgctggat accggctatc cggacgatcg cctgaaaatg atgctggaag 2041 atgcgcgtcc gtcgctgtta attaccaccg acgatcaact gccgcgcttt agcgatgttc 2101 ccaatttaac aagcctttgc tataacgccc cgcttacacc gcagggcagt gcgccgctgc 2161 aactttcaca accgcatcac acggcttata tcatctttac ctctggctcc accggcaggc 2221 cgaaaggggt aatggtcggg cagacggcta tcgtcaaccg cctgctttgg atgcaaaatc 2281 attatccgct tacaggcgaa gatgtcgttg cccaaaaaac gccgtgcagt tttgatgtct 2341 cggtgtggga gtttttctgg ccgtttatcg caggggcaaa actggtgatg gctgaaccgg 2401 aagcgcaccg cgacccgctc gctatgcagc aattctttgc cgaatatggc gtaacgacca 2461 cgcactttgt gccgtcgatg ctggcggcat ttgttgcctc gctgacgccg caaaccgctc 2521 gccagagttg cgcgacgttg aaacaggttt tctgtagtgg tgaggcctta ccggctgatt 2581 tatgccgcga atggcaacag ttaactggcg cgccgttgca taatctatat ggcccgacgg 2641 aagcggcggt agatgtcagc tggtatccgg cttttggcga ggaactggca caggtgcgcg 2701 gcagcagtgt gccgattggt tatccggtat ggaatacggg tctgcgtatt cttgatgcga 2761 tgatgcatcc ggtgccgccg ggtgtggcgg gtgatctcta tctcactggc attcaactgg 2821 cgcagggcta tctcggacgc cccgatctga ccgccagccg ctttattgcc gatccttttg 2881 ccccaggtga acggatgtac cgtaccggag acgttgcccg ctggctggat aacggcgcgg 2941 tggagtacct cgggcgcagt gatgatcagc taaaaattcg cgggcagcgt atcgaactgg 3001 gcgaaatcga tcgcgtgatg caggcgctgc cggatgtcga acaagccgtt acccacgcct 3061 gtgtgattaa ccaggcggct gccaccggtg gtgatgcgcg tcaattggtg ggctatctgg 3121 tgtcgcaatc gggcctgccg ttggatacca gcgcattgca ggcgcagctt cgtgaaacat 3181 tgccaccaca tatggtaccg gtggttctgc tgcaacttcc acagttacca cttagcgcca 3241 acggcaagct ggatcgcaaa gccttaccgt tgcctgaact gaaggcacaa gcgccagggc 3301 gtgcgccgaa agcgggcagt gaaacgatta tcgccgcggc attctcgtcg ttgctggggt 3361 gtgacgtgca ggatgccgat gctgatttct tcgcgcttgg cggtcattcg ctactggcaa 3421 tgaaactggc agcgcagtta agtcggcagg ttgcccgcca ggtgacgccg gggcaagtga 3481 tggtcgcgtc aactgtcgcc aaactggcaa cgattattga tgctgaagaa gacagcaccc 3541 ggcgtatggg attcgaaacc attctgccgt tgcgtgaagg taatggcccg acgctgtttt 3601 gtttccatcc tgcgtccggt tttgcctggc agttcagcgt gctctcgcgt tatctcgatc 3661 cacaatggtc gattatcggc attcagtcac cgcgccccaa tggccccatg cagacggcgg 3721 caaacctgga tgaagtctgc gaagcgcatc tggcaacgtt acttgaacaa caaccgcatg 3781 gcccttatta cctgctgggg tattcccttg gcggtacgct ggcgcagggt attgcggcgc 3841 gactgcgtgc ccgtggcgaa caggtggcat ttcttggctt gctggatacc tggccgccag 3901 aaacgcaaaa ctggcaggaa aaagaagcta atggtctgga cccggaagtg ctggcggaga 3961 ttaaccgcga acgcgaggcc ttcctggcag cacagcaggg aagtacttca acggagttgt 4021 ttaccaccat tgaaggcaac tacgctgatg ctgtgcgcct gctgacgact gctcatagcg 4081 taccgtttga cggtaaagcg acgctgtttg ttgctgaacg cacacttcag gaaggtatga 4141 gtcccgaacg cgcctggtcg ccgtggatag cggagctgga tatctatcgt caggattgtg 4201 cgcatgtgga tattatctct ccagggacgt ttgaaaaaat tgggccgatt attcgcgcaa 4261 cgctaaacag gtaaattaat attatttata aacccataat tacagaaaat aattatgggt 4321 tttttatttg tttgatttat aggtttgatg aatatttctc ttaaatagag tgaatgttgc 4381 attcagtgga aggaatttac aaacttcagc ttcggataat tatcaccaac tggtaaagtg 4441 agcgccgtgg agatttcccc ctattggact cattttcagg ttatacccca tgtcatcact 4501 gaatattaaa cagggaagtg acgctcattt tcccgattat cctctggcgt cgcccagtaa 4561 taatgaaatt gatttactta atctaatctc agttttatgg cgggccaaaa aaacggtcat 4621 ggcggtcgtt tttgcgtttg cctgcgcagg cttgctgatc tctttcatcc tgccgcaaaa 4681 atggaccagc gcggcggttg tcacgcctcc agaacctgtt cagtggcaag agttggagaa 4741 atcattcact aagctt //