GenBank-Updates@genbank.bio.net (02/07/91)
LOCUS AFAPHEAB 3482 bp ds-DNA BCT 07-FEB-1991
DEFINITION Plasmid pJP4 (from Pseudomonas sp. EST1001) cateachol
1,2-dioxygenase (pheB), phenol 2-monooxygenase (pheA) genes,
complete cds.
ACCESSION M57500
KEYWORDS catechol 1,2-dioxygenase; phenol monooxygenase.
SOURCE Plasmid pJP4 (from Pseudomonas sp. EST1001) DNA.
ORGANISM Plasmid pJP4
Prokaryota; Bacteria.
REFERENCE 1 (bases 1 to 3482)
AUTHORS Kivisaar,M., Kasak,L. and Nurk,A.
TITLE Expression and sequencing of the plasmid-encoded catechol 1,
2-dioxygenase gene, pheB, of phenol-degrading Pseudomonas sp.
strain EST1001
JOURNAL Unpublished (1990)
STANDARD full staff_entry
REFERENCE 2 (sites)
AUTHORS Nurk,A., Kasak,L. and Kivisaar,M.
TITLE Expression and sequence of Pseudomonas sp. EST1001 pheA gene
encoding phenol monooxygenase and its homology with the sequence of
tfdB, the structural gene for 2,4-dichlorophenol hydroxylase from
pJP4
JOURNAL Gene , 0-0 (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS 203..1111
/gene="pheB"
/codon_start=203
/product="catechol 1,2-dioxygenase"
CDS 1367..3079
/gene="pheA"
/codon_start=1367
/product="phenol monooxygenase"
BASE COUNT 889 a 913 c 885 g 795 t
ORIGIN
1 atcgataccc taaaggtaag cttgaacaat agcacccatg gcaactgctt gacgaagtct
61 gcccgtacga ctgccaagca tacttccatt gaagcgccgt ttacggtcga cttgcaagga
121 cgatagccaa aggactcacc tccattgcca atgctcgatg ggaatacatc acaacgacaa
181 taaaaatgat gagagagtaa ttatgactgt taaaatttat gacacccctg aagtgcaaga
241 ttttctaaaa atcgttgctg gtctcgacca agagggcggt aacgatcgtg gcaagcagat
301 cattcaccgc attctatctg acttgtatcg caccattgac gattttgata tcactgctga
361 gcaatactgg tctgccgtca gtctgcttaa cgctttggga caagcttcgc agtttggctt
421 gctatcaccc ggcctaggtt ttgatcatta tatggacatg cgcatggatg ccgccgatgc
481 agaagccaag cgcaccggcg gcaccccgcg taccatcgaa ggaccactgt atgttgctgg
541 tgctcctgag gcagagggtt ttgcgcgcat ggatgatgat ccagatacgg atggcgaaac
601 catgtggttg catggtcaag tgcgtgatac ggctgggaaa ccaatccccg gcgccaaagt
661 agagatttgg cactgcaact ccaaaggggg ttactccttc tttgataaga gccaaacccc
721 ttacaatttg cgccgcacca ttatcgctga caatgaaggc tactaccgtg cccgcagtgt
781 tattccatca ggctacggtg tacctgaagg cgctcctacg gatcaagtac tgaaattact
841 cggtcgccat ggcgaacgcc cagcgcatat tcactacttt atttccgcgc caggtcatca
901 gcatctaacc acgcaaatta acttggctgg cgacccctac acctatgatg actttgcttt
961 tgcaacccgt caagacttag cggcggaagg caagcgcgta gaaaaccacc cagccgcgca
1021 acaatacggc gttgaaggca ctgtgaccga agtgatattc aatattgaac tgtcgcccac
1081 tgcagaagag gagctgcaag cgcgtccata agcgtccacg tgccatggaa agcgagtcac
1141 aagaaagtca gctagccggc actgccaagg tctaatacat acaaataaac tgttgcacac
1201 cggggagcgc tgattaaggc gctcccgtaa gacaataaga attgaggttt cgaccatgac
1261 tacacagcgt aatgataatc ttgagcagcc gggccgtagc gtcatttttg atgatgggct
1321 gagcgcaact gataccccaa atgagaccaa cgtagttgaa actgaggtgt taattgtcgg
1381 ttcaggccct gctggcagct ccgcagcaat gttcctgtcg acccagggca ttagcaacat
1441 tatgatcacc aaataccgtt ggactgcgaa taccccccgt gcgcatatca ctaaccagcg
1501 caccatggaa attttacgcg acgctggtat tgaggatcag gttttagcag aagcagtccc
1561 ccatgaactt atgggtgaca cagtctattg tgagtcaatg gccggcgaag aaattggccg
1621 ccggccaact tggggcacac gacctgaccg ccgcgctgac tatgagctgg catctccagc
1681 gatgccttgc gatatcggcg gctcgatgaa tatcgccttt aaagcagact tgtcccactg
1741 gcgcccatcg gccctcgatc ctgtattggg tcttccgccc aggatcgaat atcggtggcc
1801 tcggcgctgg tttgatcgca tggtgcggcc atggaatgaa tggctggtgg tctggggttt
1861 tgatatcccg caaaccttgc ttgagcccat tatgctcaaa aatgccacca tgcgtggcac
1921 gcaaacacag ttctccactg agtatttaag ccacacccaa gacgataagg gtgtcagcgt
1981 gcaagtactc aaccgtctga ccggtcaaga atataccatt cgcgccaaat acctgattgg
2041 tgctgatggt gcgcgctcca aagtggctgc ggatatcaat caagagccac ccaagctcaa
2101 tgacgatgaa gctattcaaa tcgtgcgtaa tctagtgggt atcgaggatc ttgatgtgga
2161 aatccttggc tactcactct ggggcaataa tgaccagtac gccacgcatc tacagaaagg
2221 ccgcgtatgc tgtgccggtg atgcaatcca taagcatccg cccagtcacg gcctgggttc
2281 taatacgtca atccaagact cctacaacct gtgctggaag ttggcctgtg tactcaaagg
2341 gcaggcgggg cctgaactgt tagaaaccta ttccaccgag cgtgcaccca tcgccaagca
2401 gattgtgacg cgtgccaacg gctcgagcag tgaatataag ccgatttttg acgctttagg
2461 cgttaccgat gcgacaacca acgatgagtt tgtagaaaag cttgccttgc gtaaggaaaa
2521 ttcgcctgaa ggtgctcgcc gtcgagcagc attgcgtgcg gcgctggaca ataaggatta
2581 tgagtttaac gcccaaggca ctgaaattgg tcagttctac gactcatcag cagtgattac
2641 tgatggtcaa aaacgcccag caatgaccga ggatcctatg ctacaccacc agaaatcgac
2701 ctttcctgga ctacgcctac cccatgcatg gctaggtgat gcgaaagaga aatactccac
2761 ccatgatatt gcggagggca ctcgcttcac gattttcact ggtatcaccg gtcaagcttg
2821 ggctgatgca gcagttcgcg ttgctgagcg tttgggcatc gacttgaagg ccgtggtgat
2881 tggtgaaggg cagccggtac aagacctcta tggcgattgg ttacgccagc gtgaagtgga
2941 cgaggacggt gtgatcttgg tgcgcccaga taaacatatt ggttggcgtg cccagagtat
3001 ggtcgcagat ccagagactg cattatttga tgtactctca ggctgctgca taccaagcaa
3061 accggctctt cgcatttaag ggtgtagaaa aaatctgacc ggctggaaaa ggagtcgagg
3121 atcttagaaa atgtaagctc tcacaccaac aaaaactaag cccccgacgt gaacaatctt
3181 accttttcta gccctgatct ttccagcttt tgccaactga ataacctcgg cctgactgcc
3241 actggacaac atctttgtgc agagcgcgcc gtcatcgaat gtcgtttaac caaagcaccc
3301 gagccatgcc ccaagtgcgg ggctgctggt gtttcacgcg gtactgtcga taggcatctt
3361 gctcacacac ttacggacaa cgaccaacca gattgctgct gcgtatccgt cgctggcgtt
3421 gtgcttgcgg ctgtttctgg catgaagata caaacagtgc agcacctcca cgttcaaagc
3481 tt
//GenBank-Updates@genbank.bio.net (04/13/91)
LOCUS AFAPHEAB 3482 bp ds-DNA BCT 13-APR-1991
DEFINITION Plasmid pJP4 (from Pseudomonas sp. EST1001) cateachol 1,
2-dioxygenase (pheB), phenol 2-monooxygenase (pheA) genes, complete
cds.
ACCESSION M57500
KEYWORDS catechol 1,2-dioxygenase; phenol monooxygenase.
SOURCE Plasmid pJP4 (from Pseudomonas sp. EST1001) DNA.
ORGANISM Plasmid pJP4
Prokaryota; Bacteria.
REFERENCE 1 (sites)
AUTHORS Nurk,A., Kasak,L. and Kivisaar,M.
TITLE Expression and sequence of Pseudomonas sp. EST1001 pheA gene
encoding phenol monooxygenase and its homology with the sequence of
tfdB, the structural gene for 2,4-dichlorophenol hydroxylase from
pJP4
JOURNAL Gene , 0-0 (1991)
STANDARD full staff_review
REFERENCE 2 (bases 1 to 3482)
AUTHORS Kivisaar,M., Kasak,L. and Nurk,A.
TITLE Sequence of the plasmid-encoded catechol 1,2-dioxygenase-expressing
gene, pheB, of phenol-degrading Pseudomonas sp. strain EST1001
JOURNAL Gene 98, 15-20 (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 203..1111
/product="catechol 1,2-dioxygenase"
/gene="pheB"
/codon_start=203
CDS 1367..3079
/product="phenol monooxygenase"
/gene="pheA"
/codon_start=1367
BASE COUNT 889 a 913 c 885 g 795 t
ORIGIN
1 atcgataccc taaaggtaag cttgaacaat agcacccatg gcaactgctt gacgaagtct
61 gcccgtacga ctgccaagca tacttccatt gaagcgccgt ttacggtcga cttgcaagga
121 cgatagccaa aggactcacc tccattgcca atgctcgatg ggaatacatc acaacgacaa
181 taaaaatgat gagagagtaa ttatgactgt taaaatttat gacacccctg aagtgcaaga
241 ttttctaaaa atcgttgctg gtctcgacca agagggcggt aacgatcgtg gcaagcagat
301 cattcaccgc attctatctg acttgtatcg caccattgac gattttgata tcactgctga
361 gcaatactgg tctgccgtca gtctgcttaa cgctttggga caagcttcgc agtttggctt
421 gctatcaccc ggcctaggtt ttgatcatta tatggacatg cgcatggatg ccgccgatgc
481 agaagccaag cgcaccggcg gcaccccgcg taccatcgaa ggaccactgt atgttgctgg
541 tgctcctgag gcagagggtt ttgcgcgcat ggatgatgat ccagatacgg atggcgaaac
601 catgtggttg catggtcaag tgcgtgatac ggctgggaaa ccaatccccg gcgccaaagt
661 agagatttgg cactgcaact ccaaaggggg ttactccttc tttgataaga gccaaacccc
721 ttacaatttg cgccgcacca ttatcgctga caatgaaggc tactaccgtg cccgcagtgt
781 tattccatca ggctacggtg tacctgaagg cgctcctacg gatcaagtac tgaaattact
841 cggtcgccat ggcgaacgcc cagcgcatat tcactacttt atttccgcgc caggtcatca
901 gcatctaacc acgcaaatta acttggctgg cgacccctac acctatgatg actttgcttt
961 tgcaacccgt caagacttag cggcggaagg caagcgcgta gaaaaccacc cagccgcgca
1021 acaatacggc gttgaaggca ctgtgaccga agtgatattc aatattgaac tgtcgcccac
1081 tgcagaagag gagctgcaag cgcgtccata agcgtccacg tgccatggaa agcgagtcac
1141 aagaaagtca gctagccggc actgccaagg tctaatacat acaaataaac tgttgcacac
1201 cggggagcgc tgattaaggc gctcccgtaa gacaataaga attgaggttt cgaccatgac
1261 tacacagcgt aatgataatc ttgagcagcc gggccgtagc gtcatttttg atgatgggct
1321 gagcgcaact gataccccaa atgagaccaa cgtagttgaa actgaggtgt taattgtcgg
1381 ttcaggccct gctggcagct ccgcagcaat gttcctgtcg acccagggca ttagcaacat
1441 tatgatcacc aaataccgtt ggactgcgaa taccccccgt gcgcatatca ctaaccagcg
1501 caccatggaa attttacgcg acgctggtat tgaggatcag gttttagcag aagcagtccc
1561 ccatgaactt atgggtgaca cagtctattg tgagtcaatg gccggcgaag aaattggccg
1621 ccggccaact tggggcacac gacctgaccg ccgcgctgac tatgagctgg catctccagc
1681 gatgccttgc gatatcggcg gctcgatgaa tatcgccttt aaagcagact tgtcccactg
1741 gcgcccatcg gccctcgatc ctgtattggg tcttccgccc aggatcgaat atcggtggcc
1801 tcggcgctgg tttgatcgca tggtgcggcc atggaatgaa tggctggtgg tctggggttt
1861 tgatatcccg caaaccttgc ttgagcccat tatgctcaaa aatgccacca tgcgtggcac
1921 gcaaacacag ttctccactg agtatttaag ccacacccaa gacgataagg gtgtcagcgt
1981 gcaagtactc aaccgtctga ccggtcaaga atataccatt cgcgccaaat acctgattgg
2041 tgctgatggt gcgcgctcca aagtggctgc ggatatcaat caagagccac ccaagctcaa
2101 tgacgatgaa gctattcaaa tcgtgcgtaa tctagtgggt atcgaggatc ttgatgtgga
2161 aatccttggc tactcactct ggggcaataa tgaccagtac gccacgcatc tacagaaagg
2221 ccgcgtatgc tgtgccggtg atgcaatcca taagcatccg cccagtcacg gcctgggttc
2281 taatacgtca atccaagact cctacaacct gtgctggaag ttggcctgtg tactcaaagg
2341 gcaggcgggg cctgaactgt tagaaaccta ttccaccgag cgtgcaccca tcgccaagca
2401 gattgtgacg cgtgccaacg gctcgagcag tgaatataag ccgatttttg acgctttagg
2461 cgttaccgat gcgacaacca acgatgagtt tgtagaaaag cttgccttgc gtaaggaaaa
2521 ttcgcctgaa ggtgctcgcc gtcgagcagc attgcgtgcg gcgctggaca ataaggatta
2581 tgagtttaac gcccaaggca ctgaaattgg tcagttctac gactcatcag cagtgattac
2641 tgatggtcaa aaacgcccag caatgaccga ggatcctatg ctacaccacc agaaatcgac
2701 ctttcctgga ctacgcctac cccatgcatg gctaggtgat gcgaaagaga aatactccac
2761 ccatgatatt gcggagggca ctcgcttcac gattttcact ggtatcaccg gtcaagcttg
2821 ggctgatgca gcagttcgcg ttgctgagcg tttgggcatc gacttgaagg ccgtggtgat
2881 tggtgaaggg cagccggtac aagacctcta tggcgattgg ttacgccagc gtgaagtgga
2941 cgaggacggt gtgatcttgg tgcgcccaga taaacatatt ggttggcgtg cccagagtat
3001 ggtcgcagat ccagagactg cattatttga tgtactctca ggctgctgca taccaagcaa
3061 accggctctt cgcatttaag ggtgtagaaa aaatctgacc ggctggaaaa ggagtcgagg
3121 atcttagaaa atgtaagctc tcacaccaac aaaaactaag cccccgacgt gaacaatctt
3181 accttttcta gccctgatct ttccagcttt tgccaactga ataacctcgg cctgactgcc
3241 actggacaac atctttgtgc agagcgcgcc gtcatcgaat gtcgtttaac caaagcaccc
3301 gagccatgcc ccaagtgcgg ggctgctggt gtttcacgcg gtactgtcga taggcatctt
3361 gctcacacac ttacggacaa cgaccaacca gattgctgct gcgtatccgt cgctggcgtt
3421 gtgcttgcgg ctgtttctgg catgaagata caaacagtgc agcacctcca cgttcaaagc
3481 tt
//