GenBank-Updates@genbank.bio.net (02/23/91)
LOCUS MUSIGHCKA 1047 bp ds-DNA ROD 23-FEB-1991
DEFINITION Mouse Ig rearranged H-chain mRNA constant region.
ACCESSION M60428
KEYWORDS constant region; gamma immunoglobulin; hinge exon; immunoglobulin
heavy chain; processed gene.
SOURCE Mouse hybridoma 10D7, cDNA to mRNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1047)
AUTHORS French,D.L., Pollock,R.R., Aguila,H.L. and Scharff,M.D.
TITLE The molecular and biochemical characterization of mutant monoclonal
antibodies with increased antigen binding
JOURNAL J. Immunol. (1991) In press
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS join(1..291,292..330,331..651,652..756)
/product="Ig heavy-chain C-region"
/codon_start=1
/partial
exon 1..291
/note="'A/J IgG1 CH1 domain'"
exon 292..330
/note="'A/J IgG1 hinge'"
exon 331..651
/note="'A/J IgG1 CH2 domain'"
exon 652..972
/note="'A/J IgG1 CH3 domain'"
mutation 754..754
/type="'10D7'"
/note="'g' in mutation"
/citation=J. Immunol. ??, ??-?? (1991)
3'UTR 976..1047
BASE COUNT 262 a 320 c 243 g 222 t
ORIGIN chromosome 12.
1 gccaaaacga cacccccatc tgtctatcca ctggcccctg gatctgctgc ccaaactaac
61 tccatggtga ccctgggatg cctggtcaag ggctatttcc ctgagccagt gacagtgacc
121 tggaactctg gatccctgtc cagcggtgtg cacaccttcc cagctgtcct gcagtctgac
181 ctctacactc tgagcagctc agtgactgtc ccctccagca cctggcccag cgagaccgtc
241 acctgcaacg ttgcccaccc ggccagcagc accaaggtgg acaagaaaat tgtgcccagg
301 gattgtggtt gtaagccttg catatgtaca gtcccagaag tatcatctgt cttcatcttc
361 cccccaaagc ccaaggatgt gctcaccatt actctgactc ctaaggtcac gtgtgttgtg
421 gtagacatca gcaaggatga tcccgaggtc cagttcagct ggtttgtaga tgatgtggag
481 gtgcacacag ctcagacgca accccgggag gagcagttca acagcacttt ccgctcagtc
541 agtgaacttc ccatcatgca ccaggactgg ctcaatggca aggagttcaa atgcagggtc
601 aacagtgcag ctttccctgc ccccatcgag aaaaccatct ccaaaaccaa aggcagaccg
661 aaggctccac aggtgtacac cattccacct cccaaggagc agatggccaa ggataaagtc
721 agtctgacct gcatgataac agacttcttc ccttaagaca ttactgtgga gtggcagtgg
781 aatgggcagc cagcggagaa ctacaagaac actcagccca tcatggacac agatggctct
841 tacttcgtct acagcaagct caatgtgcag aagagcaact gggaggcagg aaatactttc
901 acctgctctg tgttacatga gggcctgcac aaccaccata ctgagaagag cctctcccac
961 tctcctggta aatgatctca gtgtccttgg agccctctgg tcctacagga ctctgacacc
1021 tacctccacc cctccctgtg taaataa
//GenBank-Updates@genbank.bio.net (02/23/91)
LOCUS MUSIGHCKB 1070 bp ds-DNA ROD 23-FEB-1991
DEFINITION Mouse Ig rearranged H-chain mRNA constant region.
ACCESSION M60429
KEYWORDS constant region; gamma immunoglobulin; hinge exon; immunoglobulin
heavy chain; processed gene.
SOURCE Mouse hybridoma 24F3, cDNA to mRNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1070)
AUTHORS French,D.L., Pollock,R.R., Aguila,H.L. and Scharff,M.D.
TITLE The molecular and biochemical characterization of mutant monoclonal
antibodies with increased antigen binding
JOURNAL J. Immunol. (1991) In press
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS join(1..291,292..330,331..651,652..750)
/product="Ig heavy-chain C-region"
/codon_start=1
/partial
exon 1..291
/note="'A/J IgG1 CH1 domain'"
exon 292..330
/note="'A/J IgG1 hinge'"
exon 331..651
/note="'A/J IgG1 CH2 domain'"
exon 652..998
/note="'23 base addition at position 652 to 674'"
/note="'A/J IgG1 CH3 domain'"
3'UTR 999..1070
BASE COUNT 269 a 328 c 252 g 221 t
ORIGIN chromosome 12.
1 gccaaaacga cacccccatc tgtctatcca ctggcccctg gatctgctgc ccaaactaac
61 tccatggtga ccctgggatg cctggtcaag ggctatttcc ctgagccagt gacagtgacc
121 tggaactctg gatccctgtc cagcggtgtg cacaccttcc cagctgtcct gcagtctgac
181 ctctacactc tgagcagctc agtgactgtc ccctccagca cctggcccag cgagaccgtc
241 acctgcaacg ttgcccaccc ggccagcagc accaaggtgg acaagaaaat tgtgcccagg
301 gattgtggtt gtaagccttg catatgtaca gtcccagaag tatcatctgt cttcatcttc
361 cccccaaagc ccaaggatgt gctcaccatt actctgactc ctaaggtcac gtgtgttgtg
421 gtagacatca gcaaggatga tcccgaggtc cagttcagct ggtttgtaga tgatgtggag
481 gtgcacacag ctcagacgca accccgggag gagcagttca acagcacttt ccgctcagtc
541 agtgaacttc ccatcatgca ccaggactgg ctcaatggca aggagttcaa atgcagggtc
601 aacagtgcag ctttccctgc ccccatcgag aaaaccatct ccaaaaccaa aggcagaccg
661 aaggcaggcc cacaggcaga ccgaaggctc cacaggtgta caccattcca cctcccaagg
721 agcagatggc caaggataaa gtcagtctga cctgcatgat aacagacttc ttccctgaag
781 acattactgt ggagtggcag tggaatgggc agccagcgga gaactacaag aacactcagc
841 ccatcatgga cacagatggc tcttacttcg tctacagcaa gctcaatgtg cagaagagca
901 actgggaggc aggaaatact ttcacctgct ctgtgttaca tgagggcctg cacaaccacc
961 atactgagaa gagcctctcc cactctcctg gtaaatgatc tcagtgtcct tggagccctc
1021 tggtcctaca ggactctgac acctacctcc acccctccct gtgtaaataa
//GenBank-Updates@genbank.bio.net (02/23/91)
LOCUS MUSIGHCKC 726 bp ds-DNA ROD 23-FEB-1991
DEFINITION Mouse Ig rearranged H-chain mRNA constant region.
ACCESSION M60430
KEYWORDS constant region; gamma immunoglobulin; hinge exon; immunoglobulin
heavy chain; processed gene.
SOURCE Mouse hybridoma 32F3, cDNA to mRNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 726)
AUTHORS French,D.L., Pollock,R.R., Aguila,H.L. and Scharff,M.D.
TITLE The molecular and biochemical characterization of mutant monoclonal
antibodies with increased antigen binding
JOURNAL J. Immunol. (1991) In press
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS join(1..291,292..330,331..654)
/product="Ig heavy-chain C-region"
/codon_start=1
/partial
exon 1..291
/note="'A/J IgG1 CH1 domain'"
exon 292..330
/note="'A/J IgG1 hinge'"
mutation 330..330
/note="CH2 domain deleted"
exon 331..654
/note="'A/J IgG1 CH3 domain'"
3'UTR (655.726)..(655.726)
BASE COUNT 178 a 227 c 170 g 151 t
ORIGIN chromosome 12.
1 gccaaaacga cacccccatc tgtctatcca ctggcccctg gatctgctgc ccaaactaac
61 tccatggtga ccctgggatg cctggtcaag ggctatttcc ctgagccagt gacagtgacc
121 tggaactctg gatccctgtc cagcggtgtg cacaccttcc cagctgtcct gcagtctgac
181 ctctacactc tgagcagctc agtgactgtc ccctccagca cctggcccag cgagaccgtc
241 acctgcaacg ttgcccaccc ggccagcagc accaaggtgg acaagaaaat tgtgcccagg
301 gattgtggtt gtaagccttg catatgtaca ggcagaccga aggctccaca ggtgtacacc
361 attccacctc ccaaggagca gatggccaag gataaagtca gtctgacctg catgataaca
421 gacttcttcc ctgaagacat tactgtggag tggcagtgga atgggcagcc agcggagaac
481 tacaagaaca ctcagcccat catggacaca gatggctctt acttcgtcta cagcaagctc
541 aatgtgcaga agagcaactg ggaggcagga aatactttca cctgctctgt gttacatgag
601 ggcctgcaca accaccatac tgagaagagc ctctcccact ctcctggtaa atgatctcag
661 tgtccttgga gccctctggt cctacaggac tctgacacct acctccaccc ctccctgtgt
721 aaataa
//GenBank-Updates@genbank.bio.net (02/23/91)
LOCUS MUSIGHCKD 1038 bp ds-DNA ROD 23-FEB-1991
DEFINITION Mouse Ig rearranged H-chain mRNA constant region.
ACCESSION M60432
KEYWORDS constant region; gamma immunoglobulin; hinge exon; immunoglobulin
heavy chain; processed gene.
SOURCE Mouse hybridoma 4F8, cDNA to mRNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1038)
AUTHORS French,D.L., Pollock,R.R., Aguila,H.L. and Scharff,M.D.
TITLE The molecular and biochemical characterization of mutant monoclonal
antibodies with increased antigen binding
JOURNAL J. Immunol. (1991) In press
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS join(1..291,292..330,331..651,652..972)
/note="stop codon and following 6 bases deleted"
/codon_start=1
/partial
exon 1..291
/note="'A/J IgG1 CH1 domain'"
exon 292..330
/note="'A/J IgG1 hinge'"
exon 331..651
/note="'A/J IgG1 CH2 domain'"
exon 652..972
/note="'A/J IgG1 CH3 domain'"
mutation 971..972
/type="'4F8'"
/note="'stop codon and following 6 bases deleted'"
/note="'aa' in mutation"
3'UTR 973..1038
BASE COUNT 258 a 318 c 242 g 220 t
ORIGIN chromosome 12.
1 gccaaaacga cacccccatc tgtctatcca ctggcccctg gatctgctgc ccaaactaac
61 tccatggtga ccctgggatg cctggtcaag ggctatttcc ctgagccagt gacagtgacc
121 tggaactctg gatccctgtc cagcggtgtg cacaccttcc cagctgtcct gcagtctgac
181 ctctacactc tgagcagctc agtgactgtc ccctccagca cctggcccag cgagaccgtc
241 acctgcaacg ttgcccaccc ggccagcagc accaaggtgg acaagaaaat tgtgcccagg
301 gattgtggtt gtaagccttg catatgtaca gtcccagaag tatcatctgt cttcatcttc
361 cccccaaagc ccaaggatgt gctcaccatt actctgactc ctaaggtcac gtgtgttgtg
421 gtagacatca gcaaggatga tcccgaggtc cagttcagct ggtttgtaga tgatgtggag
481 gtgcacacag ctcagacgca accccgggag gagcagttca acagcacttt ccgctcagtc
541 agtgaacttc ccatcatgca ccaggactgg ctcaatggca aggagttcaa atgcagggtc
601 aacagtgcag ctttccctgc ccccatcgag aaaaccatct ccaaaaccaa aggcagaccg
661 aaggctccac aggtgtacac cattccacct cccaaggagc agatggccaa ggataaagtc
721 agtctgacct gcatgataac agacttcttc cctgaagaca ttactgtgga gtggcagtgg
781 aatgggcagc cagcggagaa ctacaagaac actcagccca tcatggacac agatggctct
841 tacttcgtct acagcaagct caatgtgcag aagagcaact gggaggcagg aaatactttc
901 acctgctctg tgttacatga gggcctgcac aaccaccata ctgagaagag cctctcccac
961 tctcctggta tttgtccttg gagccctctg gtcctacagg actctgacac ctacctccac
1021 ccctccctgt gtaaataa
//GenBank-Updates@genbank.bio.net (02/23/91)
LOCUS MUSIGHCKE 1047 bp ds-DNA ROD 23-FEB-1991
DEFINITION Mouse Ig rearranged H-chain mRNA constant region.
ACCESSION M60434
KEYWORDS constant region; gamma immunoglobulin; hinge exon; immunoglobulin
heavy chain; processed gene.
SOURCE Mouse hybridoma 4H7, cDNA to mRNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1047)
AUTHORS French,D.L., Pollock,R.R., Aguila,H.L. and Scharff,M.D.
TITLE The molecular and biochemical characterization of mutant monoclonal
antibodies with increased antigen binding
JOURNAL J. Immunol. (1991) In press
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS join(1..291,292..330,331..651,652..975)
/codon_start=1
/partial
exon 1..291
/note="'A/J IgG1 CH1 domain'"
exon 292..330
/note="'A/J IgG1 hinge'"
exon 331..651
/note="'A/J IgG1 CH2 domain'"
exon 652..975
/note="'A/J IgG1 CH3 domain'"
mutation 974..974
/type="'4H7'"
/note="'g' in mutation; stop codon deleted"
/citation=J. Immunol. ??, ??-?? (1991)
3'UTR 976..1047
BASE COUNT 262 a 320 c 243 g 222 t
ORIGIN chromosome 12.
1 gccaaaacga cacccccatc tgtctatcca ctggcccctg gatctgctgc ccaaactaac
61 tccatggtga ccctgggatg cctggtcaag ggctatttcc ctgagccagt gacagtgacc
121 tggaactctg gatccctgtc cagcggtgtg cacaccttcc cagctgtcct gcagtctgac
181 ctctacactc tgagcagctc agtgactgtc ccctccagca cctggcccag cgagaccgtc
241 acctgcaacg ttgcccaccc ggccagcagc accaaggtgg acaagaaaat tgtgcccagg
301 gattgtggtt gtaagccttg catatgtaca gtcccagaag tatcatctgt cttcatcttc
361 cccccaaagc ccaaggatgt gctcaccatt actctgactc ctaaggtcac gtgtgttgtg
421 gtagacatca gcaaggatga tcccgaggtc cagttcagct ggtttgtaga tgatgtggag
481 gtgcacacag ctcagacgca accccgggag gagcagttca acagcacttt ccgctcagtc
541 agtgaacttc ccatcatgca ccaggactgg ctcaatggca aggagttcaa atgcagggtc
601 aacagtgcag ctttccctgc ccccatcgag aaaaccatct ccaaaaccaa aggcagaccg
661 aaggctccac aggtgtacac cattccacct cccaaggagc agatggccaa ggataaagtc
721 agtctgacct gcatgataac agacttcttc cctgaagaca ttactgtgga gtggcagtgg
781 aatgggcagc cagcggagaa ctacaagaac actcagccca tcatggacac agatggctct
841 tacttcgtct acagcaagct caatgtgcag aagagcaact gggaggcagg aaatactttc
901 acctgctctg tgttacatga gggcctgcac aaccaccata ctgagaagag cctctcccac
961 tctcctggta aattatctca gtgtccttgg agccctctgg tcctacagga ctctgacacc
1021 tacctccacc cctccctgtg taaataa
//GenBank-Updates@genbank.bio.net (02/23/91)
LOCUS MUSIGHCKF 1047 bp ds-DNA ROD 23-FEB-1991
DEFINITION Mouse Ig rearranged H-chain mRNA constant region.
ACCESSION M60435
KEYWORDS constant region; gamma immunoglobulin; hinge exon; immunoglobulin
heavy chain; processed gene.
SOURCE Mouse hybridoma 36-35, cDNA to mRNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1047)
AUTHORS French,D.L., Pollock,R.R., Aguila,H.L. and Scharff,M.D.
TITLE The molecular and biochemical characterization of mutant monoclonal
antibodies with increased antigen binding
JOURNAL J. Immunol. (1991) In press
STANDARD full staff_review
FEATURES Location/Qualifiers
exon 1..291
/note="'A/J IgG1 CH1 domain'"
variation 220..220
/type="'BALB/c'"
/note="'c' in variation"
/citation=J. Immunol. ??, ??-?? (1991)
variation 222..223
/type="'BALB/c'"
/note="'tc' in variation"
/citation=J. Immunol. ??, ??-?? (1991)
exon 292..330
/note="'A/J IgG1 hinge'"
exon 331..651
/note="'A/J IgG1 CH2 domain'"
exon 652..975
/note="'A/J IgG1 CH3 domain'"
variation 826..826
/type="'BALB/c'"
/note="'a' in variation"
/citation=Cell 18, 559-568 (1979)
variation 831..832
/type="'BALB/c'"
/note="'ga' in variation"
/citation=Cell 18, 559-568 (1979)
3'UTR 976..1047
variation 978..978
/type="'BALB/c'"
/note="'c' in variation"
/citation=Cell 18, 559-568 (1979)
variation 1040..1040
/type="'BALB/c'"
/note="'a' in variation"
/citation=Cell 18, 559-568 (1979)
BASE COUNT 262 a 320 c 244 g 221 t
ORIGIN chromosome 12.
1 gccaaaacga cacccccatc tgtctatcca ctggcccctg gatctgctgc ccaaactaac
61 tccatggtga ccctgggatg cctggtcaag ggctatttcc ctgagccagt gacagtgacc
121 tggaactctg gatccctgtc cagcggtgtg cacaccttcc cagctgtcct gcagtctgac
181 ctctacactc tgagcagctc agtgactgtc ccctccagca cctggcccag cgagaccgtc
241 acctgcaacg ttgcccaccc ggccagcagc accaaggtgg acaagaaaat tgtgcccagg
301 gattgtggtt gtaagccttg catatgtaca gtcccagaag tatcatctgt cttcatcttc
361 cccccaaagc ccaaggatgt gctcaccatt actctgactc ctaaggtcac gtgtgttgtg
421 gtagacatca gcaaggatga tcccgaggtc cagttcagct ggtttgtaga tgatgtggag
481 gtgcacacag ctcagacgca accccgggag gagcagttca acagcacttt ccgctcagtc
541 agtgaacttc ccatcatgca ccaggactgg ctcaatggca aggagttcaa atgcagggtc
601 aacagtgcag ctttccctgc ccccatcgag aaaaccatct ccaaaaccaa aggcagaccg
661 aaggctccac aggtgtacac cattccacct cccaaggagc agatggccaa ggataaagtc
721 agtctgacct gcatgataac agacttcttc cctgaagaca ttactgtgga gtggcagtgg
781 aatgggcagc cagcggagaa ctacaagaac actcagccca tcatggacac agatggctct
841 tacttcgtct acagcaagct caatgtgcag aagagcaact gggaggcagg aaatactttc
901 acctgctctg tgttacatga gggcctgcac aaccaccata ctgagaagag cctctcccac
961 tctcctggta aatgatctca gtgtccttgg agccctctgg tcctacagga ctctgacacc
1021 tacctccacc cctccctgtg taaataa
//GenBank-Updates@genbank.bio.net (04/13/91)
LOCUS MUSIGHCKA 1047 bp ds-DNA ROD 13-APR-1991
DEFINITION Mouse Ig rearranged H-chain mRNA constant region.
ACCESSION M60428
KEYWORDS constant region; gamma immunoglobulin; hinge exon;
immunoglobulin heavy chain; processed gene.
SOURCE Mouse hybridoma 10D7, cDNA to mRNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1047)
AUTHORS French,D.L., Pollock,R.R., Aguila,H.L. and Scharff,M.D.
TITLE The molecular and biochemical characterization of mutant monoclonal
antibodies with increased antigen binding
JOURNAL J. Immunol. 146, 2010-2016 (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS join(1..291,292..330,331..651,652..756)
/product="Ig heavy-chain C-region"
/codon_start=1
/partial
exon 1..291
/note="'A/J IgG1 CH1 domain'"
exon 292..330
/note="'A/J IgG1 hinge'"
exon 331..651
/note="'A/J IgG1 CH2 domain'"
exon 652..972
/note="'A/J IgG1 CH3 domain'"
mutation 754..754
/type="'10D7'"
/note="'g' in mutation"
/citation=J. Immunol. 146, 2010-2016 (1991)
3'UTR 976..1047
BASE COUNT 262 a 320 c 243 g 222 t
ORIGIN chromosome 12.
1 gccaaaacga cacccccatc tgtctatcca ctggcccctg gatctgctgc ccaaactaac
61 tccatggtga ccctgggatg cctggtcaag ggctatttcc ctgagccagt gacagtgacc
121 tggaactctg gatccctgtc cagcggtgtg cacaccttcc cagctgtcct gcagtctgac
181 ctctacactc tgagcagctc agtgactgtc ccctccagca cctggcccag cgagaccgtc
241 acctgcaacg ttgcccaccc ggccagcagc accaaggtgg acaagaaaat tgtgcccagg
301 gattgtggtt gtaagccttg catatgtaca gtcccagaag tatcatctgt cttcatcttc
361 cccccaaagc ccaaggatgt gctcaccatt actctgactc ctaaggtcac gtgtgttgtg
421 gtagacatca gcaaggatga tcccgaggtc cagttcagct ggtttgtaga tgatgtggag
481 gtgcacacag ctcagacgca accccgggag gagcagttca acagcacttt ccgctcagtc
541 agtgaacttc ccatcatgca ccaggactgg ctcaatggca aggagttcaa atgcagggtc
601 aacagtgcag ctttccctgc ccccatcgag aaaaccatct ccaaaaccaa aggcagaccg
661 aaggctccac aggtgtacac cattccacct cccaaggagc agatggccaa ggataaagtc
721 agtctgacct gcatgataac agacttcttc ccttaagaca ttactgtgga gtggcagtgg
781 aatgggcagc cagcggagaa ctacaagaac actcagccca tcatggacac agatggctct
841 tacttcgtct acagcaagct caatgtgcag aagagcaact gggaggcagg aaatactttc
901 acctgctctg tgttacatga gggcctgcac aaccaccata ctgagaagag cctctcccac
961 tctcctggta aatgatctca gtgtccttgg agccctctgg tcctacagga ctctgacacc
1021 tacctccacc cctccctgtg taaataa
//GenBank-Updates@genbank.bio.net (04/13/91)
LOCUS MUSIGHCKB 1070 bp ds-DNA ROD 13-APR-1991
DEFINITION Mouse Ig rearranged H-chain mRNA constant region.
ACCESSION M60429
KEYWORDS constant region; gamma immunoglobulin; hinge exon;
immunoglobulin heavy chain; processed gene.
SOURCE Mouse hybridoma 24F3, cDNA to mRNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1070)
AUTHORS French,D.L., Pollock,R.R., Aguila,H.L. and Scharff,M.D.
TITLE The molecular and biochemical characterization of mutant monoclonal
antibodies with increased antigen binding
JOURNAL J. Immunol. 146, 2010-2016 (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS join(1..291,292..330,331..651,652..750)
/product="Ig heavy-chain C-region"
/codon_start=1
/partial
exon 1..291
/note="'A/J IgG1 CH1 domain'"
exon 292..330
/note="'A/J IgG1 hinge'"
exon 331..651
/note="'A/J IgG1 CH2 domain'"
exon 652..998
/note="'23 base addition at position 652 to 674'"
/note="'A/J IgG1 CH3 domain'"
3'UTR 999..1070
BASE COUNT 269 a 328 c 252 g 221 t
ORIGIN chromosome 12.
1 gccaaaacga cacccccatc tgtctatcca ctggcccctg gatctgctgc ccaaactaac
61 tccatggtga ccctgggatg cctggtcaag ggctatttcc ctgagccagt gacagtgacc
121 tggaactctg gatccctgtc cagcggtgtg cacaccttcc cagctgtcct gcagtctgac
181 ctctacactc tgagcagctc agtgactgtc ccctccagca cctggcccag cgagaccgtc
241 acctgcaacg ttgcccaccc ggccagcagc accaaggtgg acaagaaaat tgtgcccagg
301 gattgtggtt gtaagccttg catatgtaca gtcccagaag tatcatctgt cttcatcttc
361 cccccaaagc ccaaggatgt gctcaccatt actctgactc ctaaggtcac gtgtgttgtg
421 gtagacatca gcaaggatga tcccgaggtc cagttcagct ggtttgtaga tgatgtggag
481 gtgcacacag ctcagacgca accccgggag gagcagttca acagcacttt ccgctcagtc
541 agtgaacttc ccatcatgca ccaggactgg ctcaatggca aggagttcaa atgcagggtc
601 aacagtgcag ctttccctgc ccccatcgag aaaaccatct ccaaaaccaa aggcagaccg
661 aaggcaggcc cacaggcaga ccgaaggctc cacaggtgta caccattcca cctcccaagg
721 agcagatggc caaggataaa gtcagtctga cctgcatgat aacagacttc ttccctgaag
781 acattactgt ggagtggcag tggaatgggc agccagcgga gaactacaag aacactcagc
841 ccatcatgga cacagatggc tcttacttcg tctacagcaa gctcaatgtg cagaagagca
901 actgggaggc aggaaatact ttcacctgct ctgtgttaca tgagggcctg cacaaccacc
961 atactgagaa gagcctctcc cactctcctg gtaaatgatc tcagtgtcct tggagccctc
1021 tggtcctaca ggactctgac acctacctcc acccctccct gtgtaaataa
//GenBank-Updates@genbank.bio.net (04/13/91)
LOCUS MUSIGHCKC 726 bp ds-DNA ROD 13-APR-1991
DEFINITION Mouse Ig rearranged H-chain mRNA constant region.
ACCESSION M60430
KEYWORDS constant region; gamma immunoglobulin; hinge exon;
immunoglobulin heavy chain; processed gene.
SOURCE Mouse hybridoma 32F3, cDNA to mRNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 726)
AUTHORS French,D.L., Pollock,R.R., Aguila,H.L. and Scharff,M.D.
TITLE The molecular and biochemical characterization of mutant monoclonal
antibodies with increased antigen binding
JOURNAL J. Immunol. 146, 2010-2016 (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS join(1..291,292..330,331..654)
/product="Ig heavy-chain C-region"
/codon_start=1
/partial
exon 1..291
/note="'A/J IgG1 CH1 domain'"
exon 292..330
/note="'A/J IgG1 hinge'"
mutation 330..330
/note="CH2 domain deleted"
exon 331..654
/note="'A/J IgG1 CH3 domain'"
3'UTR (655.726)..(655.726)
BASE COUNT 178 a 227 c 170 g 151 t
ORIGIN chromosome 12.
1 gccaaaacga cacccccatc tgtctatcca ctggcccctg gatctgctgc ccaaactaac
61 tccatggtga ccctgggatg cctggtcaag ggctatttcc ctgagccagt gacagtgacc
121 tggaactctg gatccctgtc cagcggtgtg cacaccttcc cagctgtcct gcagtctgac
181 ctctacactc tgagcagctc agtgactgtc ccctccagca cctggcccag cgagaccgtc
241 acctgcaacg ttgcccaccc ggccagcagc accaaggtgg acaagaaaat tgtgcccagg
301 gattgtggtt gtaagccttg catatgtaca ggcagaccga aggctccaca ggtgtacacc
361 attccacctc ccaaggagca gatggccaag gataaagtca gtctgacctg catgataaca
421 gacttcttcc ctgaagacat tactgtggag tggcagtgga atgggcagcc agcggagaac
481 tacaagaaca ctcagcccat catggacaca gatggctctt acttcgtcta cagcaagctc
541 aatgtgcaga agagcaactg ggaggcagga aatactttca cctgctctgt gttacatgag
601 ggcctgcaca accaccatac tgagaagagc ctctcccact ctcctggtaa atgatctcag
661 tgtccttgga gccctctggt cctacaggac tctgacacct acctccaccc ctccctgtgt
721 aaataa
//GenBank-Updates@genbank.bio.net (04/13/91)
LOCUS MUSIGHCKD 1038 bp ds-DNA ROD 13-APR-1991
DEFINITION Mouse Ig rearranged H-chain mRNA constant region.
ACCESSION M60432
KEYWORDS constant region; gamma immunoglobulin; hinge exon;
immunoglobulin heavy chain; processed gene.
SOURCE Mouse hybridoma 4F8, cDNA to mRNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1038)
AUTHORS French,D.L., Pollock,R.R., Aguila,H.L. and Scharff,M.D.
TITLE The molecular and biochemical characterization of mutant monoclonal
antibodies with increased antigen binding
JOURNAL J. Immunol. 146, 2010-2016 (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS join(1..291,292..330,331..651,652..972)
/note="stop codon and following 6 bases deleted"
/codon_start=1
/partial
exon 1..291
/note="'A/J IgG1 CH1 domain'"
exon 292..330
/note="'A/J IgG1 hinge'"
exon 331..651
/note="'A/J IgG1 CH2 domain'"
exon 652..972
/note="'A/J IgG1 CH3 domain'"
mutation 971..972
/type="'4F8'"
/note="'stop codon and following 6 bases deleted'"
/note="'aa' in mutation"
3'UTR 973..1038
BASE COUNT 258 a 318 c 242 g 220 t
ORIGIN chromosome 12.
1 gccaaaacga cacccccatc tgtctatcca ctggcccctg gatctgctgc ccaaactaac
61 tccatggtga ccctgggatg cctggtcaag ggctatttcc ctgagccagt gacagtgacc
121 tggaactctg gatccctgtc cagcggtgtg cacaccttcc cagctgtcct gcagtctgac
181 ctctacactc tgagcagctc agtgactgtc ccctccagca cctggcccag cgagaccgtc
241 acctgcaacg ttgcccaccc ggccagcagc accaaggtgg acaagaaaat tgtgcccagg
301 gattgtggtt gtaagccttg catatgtaca gtcccagaag tatcatctgt cttcatcttc
361 cccccaaagc ccaaggatgt gctcaccatt actctgactc ctaaggtcac gtgtgttgtg
421 gtagacatca gcaaggatga tcccgaggtc cagttcagct ggtttgtaga tgatgtggag
481 gtgcacacag ctcagacgca accccgggag gagcagttca acagcacttt ccgctcagtc
541 agtgaacttc ccatcatgca ccaggactgg ctcaatggca aggagttcaa atgcagggtc
601 aacagtgcag ctttccctgc ccccatcgag aaaaccatct ccaaaaccaa aggcagaccg
661 aaggctccac aggtgtacac cattccacct cccaaggagc agatggccaa ggataaagtc
721 agtctgacct gcatgataac agacttcttc cctgaagaca ttactgtgga gtggcagtgg
781 aatgggcagc cagcggagaa ctacaagaac actcagccca tcatggacac agatggctct
841 tacttcgtct acagcaagct caatgtgcag aagagcaact gggaggcagg aaatactttc
901 acctgctctg tgttacatga gggcctgcac aaccaccata ctgagaagag cctctcccac
961 tctcctggta tttgtccttg gagccctctg gtcctacagg actctgacac ctacctccac
1021 ccctccctgt gtaaataa
//GenBank-Updates@genbank.bio.net (04/13/91)
LOCUS MUSIGHCKE 1047 bp ds-DNA ROD 13-APR-1991
DEFINITION Mouse Ig rearranged H-chain mRNA constant region.
ACCESSION M60434
KEYWORDS constant region; gamma immunoglobulin; hinge exon;
immunoglobulin heavy chain; processed gene.
SOURCE Mouse hybridoma 4H7, cDNA to mRNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1047)
AUTHORS French,D.L., Pollock,R.R., Aguila,H.L. and Scharff,M.D.
TITLE The molecular and biochemical characterization of mutant monoclonal
antibodies with increased antigen binding
JOURNAL J. Immunol. 146, 2010-2016 (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
CDS join(1..291,292..330,331..651,652..975)
/codon_start=1
/partial
exon 1..291
/note="'A/J IgG1 CH1 domain'"
exon 292..330
/note="'A/J IgG1 hinge'"
exon 331..651
/note="'A/J IgG1 CH2 domain'"
exon 652..975
/note="'A/J IgG1 CH3 domain'"
mutation 974..974
/type="'4H7'"
/note="'g' in mutation; stop codon deleted"
/citation=J. Immunol. 146, 2010-2016 (1991)
3'UTR 976..1047
BASE COUNT 262 a 320 c 243 g 222 t
ORIGIN chromosome 12.
1 gccaaaacga cacccccatc tgtctatcca ctggcccctg gatctgctgc ccaaactaac
61 tccatggtga ccctgggatg cctggtcaag ggctatttcc ctgagccagt gacagtgacc
121 tggaactctg gatccctgtc cagcggtgtg cacaccttcc cagctgtcct gcagtctgac
181 ctctacactc tgagcagctc agtgactgtc ccctccagca cctggcccag cgagaccgtc
241 acctgcaacg ttgcccaccc ggccagcagc accaaggtgg acaagaaaat tgtgcccagg
301 gattgtggtt gtaagccttg catatgtaca gtcccagaag tatcatctgt cttcatcttc
361 cccccaaagc ccaaggatgt gctcaccatt actctgactc ctaaggtcac gtgtgttgtg
421 gtagacatca gcaaggatga tcccgaggtc cagttcagct ggtttgtaga tgatgtggag
481 gtgcacacag ctcagacgca accccgggag gagcagttca acagcacttt ccgctcagtc
541 agtgaacttc ccatcatgca ccaggactgg ctcaatggca aggagttcaa atgcagggtc
601 aacagtgcag ctttccctgc ccccatcgag aaaaccatct ccaaaaccaa aggcagaccg
661 aaggctccac aggtgtacac cattccacct cccaaggagc agatggccaa ggataaagtc
721 agtctgacct gcatgataac agacttcttc cctgaagaca ttactgtgga gtggcagtgg
781 aatgggcagc cagcggagaa ctacaagaac actcagccca tcatggacac agatggctct
841 tacttcgtct acagcaagct caatgtgcag aagagcaact gggaggcagg aaatactttc
901 acctgctctg tgttacatga gggcctgcac aaccaccata ctgagaagag cctctcccac
961 tctcctggta aattatctca gtgtccttgg agccctctgg tcctacagga ctctgacacc
1021 tacctccacc cctccctgtg taaataa
//GenBank-Updates@genbank.bio.net (04/13/91)
LOCUS MUSIGHCKF 1047 bp ds-DNA ROD 13-APR-1991
DEFINITION Mouse Ig rearranged H-chain mRNA constant region.
ACCESSION M60435
KEYWORDS constant region; gamma immunoglobulin; hinge exon;
immunoglobulin heavy chain; processed gene.
SOURCE Mouse hybridoma 36-35, cDNA to mRNA.
ORGANISM Mus musculus
Eukaryota; Animalia; Metazoa; Chordata; Vertebrata; Mammalia;
Theria; Eutheria; Rodentia; Myomorpha; Muridae; Murinae.
REFERENCE 1 (bases 1 to 1047)
AUTHORS French,D.L., Pollock,R.R., Aguila,H.L. and Scharff,M.D.
TITLE The molecular and biochemical characterization of mutant monoclonal
antibodies with increased antigen binding
JOURNAL J. Immunol. 146, 2010-2016 (1991)
STANDARD full staff_review
FEATURES Location/Qualifiers
exon 1..291
/note="'A/J IgG1 CH1 domain'"
variation 220..220
/type="'BALB/c'"
/note="'c' in variation"
/citation=J. Immunol. 146, 2010-2016 (1991)
/citation=Cell 18, 559-568 (1979)
variation 222..223
/type="'BALB/c'"
/note="'tc' in variation"
/citation=J. Immunol. 146, 2010-2016 (1991)
/citation=Cell 18, 559-568 (1979)
exon 292..330
/note="'A/J IgG1 hinge'"
exon 331..651
/note="'A/J IgG1 CH2 domain'"
exon 652..975
/note="'A/J IgG1 CH3 domain'"
variation 826..826
/type="'BALB/c'"
/note="'a' in variation"
/citation=Cell 18, 559-568 (1979)
variation 831..832
/type="'BALB/c'"
/note="'ga' in variation"
/citation=Cell 18, 559-568 (1979)
3'UTR 976..1047
variation 978..978
/type="'BALB/c'"
/note="'c' in variation"
/citation=Cell 18, 559-568 (1979)
variation 1040..1040
/type="'BALB/c'"
/note="'a' in variation"
/citation=Cell 18, 559-568 (1979)
BASE COUNT 262 a 320 c 244 g 221 t
ORIGIN chromosome 12.
1 gccaaaacga cacccccatc tgtctatcca ctggcccctg gatctgctgc ccaaactaac
61 tccatggtga ccctgggatg cctggtcaag ggctatttcc ctgagccagt gacagtgacc
121 tggaactctg gatccctgtc cagcggtgtg cacaccttcc cagctgtcct gcagtctgac
181 ctctacactc tgagcagctc agtgactgtc ccctccagca cctggcccag cgagaccgtc
241 acctgcaacg ttgcccaccc ggccagcagc accaaggtgg acaagaaaat tgtgcccagg
301 gattgtggtt gtaagccttg catatgtaca gtcccagaag tatcatctgt cttcatcttc
361 cccccaaagc ccaaggatgt gctcaccatt actctgactc ctaaggtcac gtgtgttgtg
421 gtagacatca gcaaggatga tcccgaggtc cagttcagct ggtttgtaga tgatgtggag
481 gtgcacacag ctcagacgca accccgggag gagcagttca acagcacttt ccgctcagtc
541 agtgaacttc ccatcatgca ccaggactgg ctcaatggca aggagttcaa atgcagggtc
601 aacagtgcag ctttccctgc ccccatcgag aaaaccatct ccaaaaccaa aggcagaccg
661 aaggctccac aggtgtacac cattccacct cccaaggagc agatggccaa ggataaagtc
721 agtctgacct gcatgataac agacttcttc cctgaagaca ttactgtgga gtggcagtgg
781 aatgggcagc cagcggagaa ctacaagaac actcagccca tcatggacac agatggctct
841 tacttcgtct acagcaagct caatgtgcag aagagcaact gggaggcagg aaatactttc
901 acctgctctg tgttacatga gggcctgcac aaccaccata ctgagaagag cctctcccac
961 tctcctggta aatgatctca gtgtccttgg agccctctgg tcctacagga ctctgacacc
1021 tacctccacc cctccctgtg taaataa
//