GenBank-Updates@genbank.bio.net (02/23/91)
LOCUS PSEBRACG 5302 bp ds-DNA BCT 23-FEB-1991
DEFINITION P.aeruginosa braCDEFG operon for branched-chain amino acid
transport (LIV-I) components.
ACCESSION D90223 M60185
KEYWORDS ATP-binding protein; braCDEFG operon; branched-chain amino acid;
carrier protein.
SOURCE P.aeruginosa (strain PAO) genomic DNA.
ORGANISM Pseudomonas aeruginosa
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (sites)
AUTHORS Hoshino,T. and Kose,K.
TITLE Cloning, nucleotide sequences, and identification of products of
the Pseudomonas aeruginosas PAO bra genes, which encode the
high-affinity branched-chain amino acid transport system
JOURNAL J. Bacteriol. 172, 5531-5539 (1990)
STANDARD full staff_review
COMMENT These data kindly submitted in computer readable form by:
Toshimitsu Hoshino
Mitsubishi Kasei Institute of Life Sciences
11, Minamiooya
Machida-shi
Tokyo 194
Japan
Phone: 001-81-427-24-6253
Fax: 001-81-427-29-1252
FEATURES Location/Qualifiers
CDS 156..1277
/note="BraC (periplasmic protein)"
CDS 1538..2461
/note="BraD (integral membrane protein)"
CDS 2458..3711
/note="BraE (integral membrane protein)"
CDS 3708..4475
/note="BraF (peripheral membrane protein, ATP-binding
protein)"
CDS 4478..5179
/note="BraG (peripheral membrane protein, ATP-binding
protein)"
BASE COUNT 962 a 1821 c 1610 g 909 t
ORIGIN 64 min on P.aeruginosa map.
1 gagctcgccg tgccgtgctt ttcaagcgac gaagcgccgc tctacactgt accggtacag
61 ttcccggggt ccgtccatga cgatcacccg gtctcctccg gcccccgcgg ccgcgagact
121 acaacaatga caacacacaa gaagagtgga gcactatgaa gaagggtact cagcgtctat
181 cccgcctgtt cgccgcgatg gccattgccg ggttcgccag ctactccatg gccgccgaca
241 ccatcaagat cgccctggct ggcccggtca ccggtccggt agcccagtac ggcgacatgc
301 agcgcgccgg tgcgctgatg gcaatcgaac agatcaacaa ggcaggcggc gtgaacggcg
361 cgcaactcga aggcgtgatc tacgacgacg cctgcgatcc caagcaggcc gtggcggtcg
421 ccaacaaggt ggtcaacgac ggcgtcaagt tcgtggtcgg tcatgtctgc tccagctcca
481 cccaacccgc caccgacatc tacgaagacg aaggcgtgct gatgatcacc ccgtcggcca
541 ccgccccgga aatcacctcg cgcggctaca agctgatctt ccgcaccatc ggcctggaca
601 acatgcaggg cccggtggcc ggcaagttca tcgccgaacg ctacaaggac aagaccatcg
661 cggtactgca cgacaagcag cagtacggcg aaggcatcgc caccgaggtg aagaagaccg
721 tggaagacgc cggcatcaag gttgccgtct tcgaaggcct gaacgccggc gacaaggact
781 tcaacgcgct gatcagcaag ctgaagaaag ccggcgtgca gttcgtctac ttcggcggct
841 accacccaga aatgggcctg ctgctgcgcc aggccaagca ggccgggctg gacgcgcgct
901 tcatgggccc ggaaggggtc ggcaacagcg aaatcaccgc gatcgccggc gacgcttcgg
961 aaggcatgct ggcgaccctg ccgcgcgcct tcgagcagga tccgaagaac aaggccctga
1021 tcgacgcctt caaggcgaag aaccaggatc cgagcggcat cttcgtcctg cccgcctact
1081 ccgcggtcac agtgatcgcc aagggcatcg agaaagccgg cgaggccgat ccggagaagg
1141 tcgccgaggc cctgcgcgcc aacaccttcg agactcccac cgggaacctc gggttcgacg
1201 agaagggcga cctgaagaac ttcgacttca ccgtctacga gtggcacaag gacgccaccc
1261 ggaccgaggt caagtaagca tcgttgcagt acggagccca cgtcacccac cgtgggcttt
1321 gttttagtac ccgcatgatt ccgcgagcgg cggcccggca agggcaggcg aagaccggca
1381 gcgttcactc ctggtggacg ggagcgcggc tttcccctgc ctcccgccgg cccgccgcgc
1441 gccggatcgt gacgggtctc agcagcgttc cggccccaca cccgcgagcc gaacgtctac
1501 ctgccgtaca acggcgggcg tttagcgaga gagtcccatg cccgagattt atcattacct
1561 acaacaattg gttaacggcc tgaccgtcgg cagcacctat gcgctgatcg cgatcggcta
1621 caccatggtc tacggcatca tcggcatgat caacttcgcc cacggcgagg tgtacatgat
1681 cggctcgtac atcgccttca tcgccatcac cctgctggcg atgatggggc tggacagcgt
1741 tccgctgatg atgctcgccg cattcgccgc cagcatcatc gtcaccagtg ctttcggcta
1801 cagcatcgag cgggtcgcct accgcccgtt gcgcggcggc aaccgcctga tcccgctgat
1861 ctccgcgatc ggcatgtcga tcttcctgca gaacgccgtg atgctctcgc aggactccaa
1921 ggaaaaggcc atcccgaccc tgctgcccgg caacttcgtg ttcggcgaaa gcagcatgaa
1981 cggcgtggtg atctcctata tgcagatcct gatcttcgtc gtcaccttcc tggtgatgtt
2041 cggcctcacc ctgttcatct cccgttcccg cctgggccgc gcctgccgcg cctgcgccga
2101 ggacctgaag atgaccaacc tgctgggtat caacagcaac aacatcatcg ccctcacctt
2161 cgtcatcggc gccgccctgg ccgccgtcgc cgcggtgctg ctgggcatgc agtacggcgt
2221 gatcaacccc gggatcggct tcctcgccgg gatcaaggcg ttcaccgccg cggtgctcgg
2281 cggcatcggt agcatccccg gcgccatgct cggcggcctg ctgctgggcg tcgccgaagc
2341 cttcggcgcc gacgtgttcg gcgaccagta caaggacgtg gtggccttcg gcctgctgat
2401 cctggtgctg ctgttccgac ctaccggcat cctcggtcgt ccggaggtgg aaaaagtatg
2461 agccagtccc tcaagcgcgc gctgttcagc gccctcctcg tgatcctggt gtcctatccg
2521 atcctaggcc tgaagctgcg caccgtcggc atcaagctcg aggtcctcgg cgccgatgcg
2581 cagaccctct ggaccatcgc cgcggccgcc ctggccatgt tcgtctggca gctgttccgc
2641 gaccgcatcc cgctcaagct gggtcgcggc gtcggctaca aggtcaacgg cagcggcctg
2701 aagaacttcc tcagcctgcc atcgacccag cgctgggcgg tcctcgccct ggtcgtggtg
2761 gctttcgtct ggccgttctt cgcctcgcgc ggggcggtgg acatcgccac cctgatcctg
2821 atctacgtga tgctcggcat cggcctgaac atcgtggtcg gcctggccgg cctgctcgat
2881 ctcggctacg tcggcttcta cgcggtaggt gcctacacct acgcgctgct cgccgagtac
2941 gccgggttcg gcttctggac cgccctgccg atcgccggga tgatggccgc gctgttcggc
3001 ttcctcctcg gcttcccggt gctgcgcctg cgcggcgact acctggcgat cgtgaccctc
3061 ggcttcggcg agatcatccg catcctgctg cgcaacatga ccgagatcac cggcggcccc
3121 aacggcatcg gctcgatccc caagccgacc ctgttcggcc tgaccttcga acgccgcgcg
3181 ccggaaggca tgcagacctt ccacgagttc ttcggcatcg cctacaacac caactacaag
3241 gtcatcctgc tctacgtggt ggccctgctg ctggtgctgc tggccctgtt cgtgatcaac
3301 cggctgatgc gcatgccgat cggtcgcgcc tgggaagcgc tgcgcgagga cgaagtggcc
3361 tgccgcgctc tcggtctcaa cccgaccatc gtcaagctct ccgccttcac catcggcgcc
3421 agcttcgccg gtttcgccgg cagcttcttc gccgcccgcc agggcctggt gacgcctgag
3481 tccttcacct tcatcgagtc ggcgatgatc cttgcgatcg tcgtgctcgg cggcatgggt
3541 tcgcagctcg gcgtgatact cgccgcggtg gtgatggtgc tgctccagga aatgcgcggg
3601 ttcaacgaat accgcatgct gatcttcggc ctgaccatga tcgtgatgat gatctggcgt
3661 ccccagggac tgctgccgat gcaacgcccg cacctggagc tgaagccatg agccgaccga
3721 ttctcgaagt gagcggcctg accatgcgct tcggcggcct gctggccgtc aacggcgtca
3781 acctgaaggt cgaggaaaag caggtggtct cgatgatcgg cccgaacggc gccggcaaga
3841 ccaccgtgtt caactgcctg accggcttct accagcccac cggcggcctg atccgcctcg
3901 acggcgagga gatccagggc ctgcccggtc acaagatcgc tcgcaagggc gtggtgcgga
3961 ccttccagaa cgtccgcctg ttcaaggaaa tgaccgcggt ggagaacctg ctggtcgccc
4021 agcaccgcca cctcaacacc aacttcctcg ccggcctgtt caagaccccg gcattccgcc
4081 gcagcgagcg cgaggccatg gagtacgccg cgcactggct ggaggaagtc aaccttaccg
4141 agttcgccaa ccgcagcgcc ggcaccctcg cctatggcca gcagcgacgc ctggagatcg
4201 cccgctgcat gatgacccgc ccgcggatcc tcatgctcga cgagccggcc gccggcctca
4261 acccgaagga gaccgacgac ctcaaggcgc tgatcgccaa gctgcgcagc gagcacaacg
4321 tgacggtatt gctgatcgag cacgacatga agctggtgat gagcatttcc gaccatatcg
4381 tggtgatcaa ccagggcgcc cccctggccg acgggacgcc ggagcagatc cgcgacaacc
4441 cggacgtgat caaggcttat ctgggggaag cgtgagcatg ctgagtttcg acaaggtttc
4501 cacctactac ggcaagatcc aggcgctgca cgacgtcagc gtggaagtga agaagggcga
4561 gatcgtcacc ctgatcggcg ccaacggcgc cggcaagtcg accctgctga tgacgctctg
4621 cggctcgccg caggcggcca gcggcagcat ccgctacgaa ggcgaggaac tggtcggcct
4681 gccctcctcc accatcatgc gcaagagcat cgcggtggtc ccggaaggcc gccgggtctt
4741 ctcccgcctg acggtggaag aaaacctggc gatgggcggc ttcttcaccg acaaggacga
4801 ctaccaggtg cagatggaca aggtgctgga actcttcccg cgcctgaagg aacgctacga
4861 acagcgcgcc gggaccatgt ccggcggcga acagcagatg ctcgccatcg gccgcgcgct
4921 gatgagcaag ccaaagctgt tgctgttgga cgaaccttcg ctcggcctgg cgccgatcat
4981 catccagcag atcttcgaga tcatcgagca gttgcgtcgc gaaggcgtca ccgtgttcct
5041 cgtcgagcag aacgccaacc aggcgttgaa gctcgccgat cgcgcctacg tgctggagaa
5101 cggccggatc gtcatgcacg acaccggcgc cgccctgctg accaacccga aggtgcgcga
5161 cgcctacctc ggcggctgag caccgtcccg ccggagactt cagcgcatac gaaaacggcc
5221 ctcacgggcc gttttttatg ccgaggcgta ccggctacag gccgtagcgc tcgatagcgt
5281 ccaggtgcgg ctgggtgtcg ac
//GenBank-Updates@genbank.bio.net (04/19/91)
LOCUS PSEBRACG 5302 bp ds-DNA BCT 19-APR-1991
DEFINITION P.aeruginosa braCDEFG operon for branched-chain amino acid
transport (LIV-I) components.
ACCESSION D90223 M60185
KEYWORDS ATP-binding protein; braCDEFG operon; branched-chain amino acid;
carrier protein.
SOURCE P.aeruginosa (strain PAO) genomic DNA.
ORGANISM Pseudomonas aeruginosa
Prokaryota; Bacteria; Gracilicutes; Scotobacteria;
Pseudomonadaceae.
REFERENCE 1 (bases 1 to 5302)
AUTHORS Hoshino,T. and Kose,K.
TITLE Cloning, nucleotide sequences, and identification of products of
the Pseudomonas aeruginosas PAO bra genes, which encode the
high-affinity branched-chain amino acid transport system
JOURNAL J. Bacteriol. 172, 5531-5539 (1990)
STANDARD full staff_entry
COMMENT These data kindly submitted in computer readable form by:
Toshimitsu Hoshino
Mitsubishi Kasei Institute of Life Sciences
11, Minamiooya
Machida-shi
Tokyo 194
Japan
Phone: 001-81-427-24-6253
Fax: 001-81-427-29-1252
FEATURES Location/Qualifiers
CDS 156..1277
/note="BraC (periplasmic protein)"
CDS 1538..2461
/note="BraD (integral membrane protein)"
CDS 2458..3711
/note="BraE (integral membrane protein)"
CDS 3708..4475
/note="BraF (peripheral membrane protein, ATP-binding
protein)"
CDS 4478..5179
/note="BraG (peripheral membrane protein, ATP-binding
protein)"
BASE COUNT 962 a 1821 c 1610 g 909 t
ORIGIN 64 min on P.aeruginosa map.
1 gagctcgccg tgccgtgctt ttcaagcgac gaagcgccgc tctacactgt accggtacag
61 ttcccggggt ccgtccatga cgatcacccg gtctcctccg gcccccgcgg ccgcgagact
121 acaacaatga caacacacaa gaagagtgga gcactatgaa gaagggtact cagcgtctat
181 cccgcctgtt cgccgcgatg gccattgccg ggttcgccag ctactccatg gccgccgaca
241 ccatcaagat cgccctggct ggcccggtca ccggtccggt agcccagtac ggcgacatgc
301 agcgcgccgg tgcgctgatg gcaatcgaac agatcaacaa ggcaggcggc gtgaacggcg
361 cgcaactcga aggcgtgatc tacgacgacg cctgcgatcc caagcaggcc gtggcggtcg
421 ccaacaaggt ggtcaacgac ggcgtcaagt tcgtggtcgg tcatgtctgc tccagctcca
481 cccaacccgc caccgacatc tacgaagacg aaggcgtgct gatgatcacc ccgtcggcca
541 ccgccccgga aatcacctcg cgcggctaca agctgatctt ccgcaccatc ggcctggaca
601 acatgcaggg cccggtggcc ggcaagttca tcgccgaacg ctacaaggac aagaccatcg
661 cggtactgca cgacaagcag cagtacggcg aaggcatcgc caccgaggtg aagaagaccg
721 tggaagacgc cggcatcaag gttgccgtct tcgaaggcct gaacgccggc gacaaggact
781 tcaacgcgct gatcagcaag ctgaagaaag ccggcgtgca gttcgtctac ttcggcggct
841 accacccaga aatgggcctg ctgctgcgcc aggccaagca ggccgggctg gacgcgcgct
901 tcatgggccc ggaaggggtc ggcaacagcg aaatcaccgc gatcgccggc gacgcttcgg
961 aaggcatgct ggcgaccctg ccgcgcgcct tcgagcagga tccgaagaac aaggccctga
1021 tcgacgcctt caaggcgaag aaccaggatc cgagcggcat cttcgtcctg cccgcctact
1081 ccgcggtcac agtgatcgcc aagggcatcg agaaagccgg cgaggccgat ccggagaagg
1141 tcgccgaggc cctgcgcgcc aacaccttcg agactcccac cgggaacctc gggttcgacg
1201 agaagggcga cctgaagaac ttcgacttca ccgtctacga gtggcacaag gacgccaccc
1261 ggaccgaggt caagtaagca tcgttgcagt acggagccca cgtcacccac cgtgggcttt
1321 gttttagtac ccgcatgatt ccgcgagcgg cggcccggca agggcaggcg aagaccggca
1381 gcgttcactc ctggtggacg ggagcgcggc tttcccctgc ctcccgccgg cccgccgcgc
1441 gccggatcgt gacgggtctc agcagcgttc cggccccaca cccgcgagcc gaacgtctac
1501 ctgccgtaca acggcgggcg tttagcgaga gagtcccatg cccgagattt atcattacct
1561 acaacaattg gttaacggcc tgaccgtcgg cagcacctat gcgctgatcg cgatcggcta
1621 caccatggtc tacggcatca tcggcatgat caacttcgcc cacggcgagg tgtacatgat
1681 cggctcgtac atcgccttca tcgccatcac cctgctggcg atgatggggc tggacagcgt
1741 tccgctgatg atgctcgccg cattcgccgc cagcatcatc gtcaccagtg ctttcggcta
1801 cagcatcgag cgggtcgcct accgcccgtt gcgcggcggc aaccgcctga tcccgctgat
1861 ctccgcgatc ggcatgtcga tcttcctgca gaacgccgtg atgctctcgc aggactccaa
1921 ggaaaaggcc atcccgaccc tgctgcccgg caacttcgtg ttcggcgaaa gcagcatgaa
1981 cggcgtggtg atctcctata tgcagatcct gatcttcgtc gtcaccttcc tggtgatgtt
2041 cggcctcacc ctgttcatct cccgttcccg cctgggccgc gcctgccgcg cctgcgccga
2101 ggacctgaag atgaccaacc tgctgggtat caacagcaac aacatcatcg ccctcacctt
2161 cgtcatcggc gccgccctgg ccgccgtcgc cgcggtgctg ctgggcatgc agtacggcgt
2221 gatcaacccc gggatcggct tcctcgccgg gatcaaggcg ttcaccgccg cggtgctcgg
2281 cggcatcggt agcatccccg gcgccatgct cggcggcctg ctgctgggcg tcgccgaagc
2341 cttcggcgcc gacgtgttcg gcgaccagta caaggacgtg gtggccttcg gcctgctgat
2401 cctggtgctg ctgttccgac ctaccggcat cctcggtcgt ccggaggtgg aaaaagtatg
2461 agccagtccc tcaagcgcgc gctgttcagc gccctcctcg tgatcctggt gtcctatccg
2521 atcctaggcc tgaagctgcg caccgtcggc atcaagctcg aggtcctcgg cgccgatgcg
2581 cagaccctct ggaccatcgc cgcggccgcc ctggccatgt tcgtctggca gctgttccgc
2641 gaccgcatcc cgctcaagct gggtcgcggc gtcggctaca aggtcaacgg cagcggcctg
2701 aagaacttcc tcagcctgcc atcgacccag cgctgggcgg tcctcgccct ggtcgtggtg
2761 gctttcgtct ggccgttctt cgcctcgcgc ggggcggtgg acatcgccac cctgatcctg
2821 atctacgtga tgctcggcat cggcctgaac atcgtggtcg gcctggccgg cctgctcgat
2881 ctcggctacg tcggcttcta cgcggtaggt gcctacacct acgcgctgct cgccgagtac
2941 gccgggttcg gcttctggac cgccctgccg atcgccggga tgatggccgc gctgttcggc
3001 ttcctcctcg gcttcccggt gctgcgcctg cgcggcgact acctggcgat cgtgaccctc
3061 ggcttcggcg agatcatccg catcctgctg cgcaacatga ccgagatcac cggcggcccc
3121 aacggcatcg gctcgatccc caagccgacc ctgttcggcc tgaccttcga acgccgcgcg
3181 ccggaaggca tgcagacctt ccacgagttc ttcggcatcg cctacaacac caactacaag
3241 gtcatcctgc tctacgtggt ggccctgctg ctggtgctgc tggccctgtt cgtgatcaac
3301 cggctgatgc gcatgccgat cggtcgcgcc tgggaagcgc tgcgcgagga cgaagtggcc
3361 tgccgcgctc tcggtctcaa cccgaccatc gtcaagctct ccgccttcac catcggcgcc
3421 agcttcgccg gtttcgccgg cagcttcttc gccgcccgcc agggcctggt gacgcctgag
3481 tccttcacct tcatcgagtc ggcgatgatc cttgcgatcg tcgtgctcgg cggcatgggt
3541 tcgcagctcg gcgtgatact cgccgcggtg gtgatggtgc tgctccagga aatgcgcggg
3601 ttcaacgaat accgcatgct gatcttcggc ctgaccatga tcgtgatgat gatctggcgt
3661 ccccagggac tgctgccgat gcaacgcccg cacctggagc tgaagccatg agccgaccga
3721 ttctcgaagt gagcggcctg accatgcgct tcggcggcct gctggccgtc aacggcgtca
3781 acctgaaggt cgaggaaaag caggtggtct cgatgatcgg cccgaacggc gccggcaaga
3841 ccaccgtgtt caactgcctg accggcttct accagcccac cggcggcctg atccgcctcg
3901 acggcgagga gatccagggc ctgcccggtc acaagatcgc tcgcaagggc gtggtgcgga
3961 ccttccagaa cgtccgcctg ttcaaggaaa tgaccgcggt ggagaacctg ctggtcgccc
4021 agcaccgcca cctcaacacc aacttcctcg ccggcctgtt caagaccccg gcattccgcc
4081 gcagcgagcg cgaggccatg gagtacgccg cgcactggct ggaggaagtc aaccttaccg
4141 agttcgccaa ccgcagcgcc ggcaccctcg cctatggcca gcagcgacgc ctggagatcg
4201 cccgctgcat gatgacccgc ccgcggatcc tcatgctcga cgagccggcc gccggcctca
4261 acccgaagga gaccgacgac ctcaaggcgc tgatcgccaa gctgcgcagc gagcacaacg
4321 tgacggtatt gctgatcgag cacgacatga agctggtgat gagcatttcc gaccatatcg
4381 tggtgatcaa ccagggcgcc cccctggccg acgggacgcc ggagcagatc cgcgacaacc
4441 cggacgtgat caaggcttat ctgggggaag cgtgagcatg ctgagtttcg acaaggtttc
4501 cacctactac ggcaagatcc aggcgctgca cgacgtcagc gtggaagtga agaagggcga
4561 gatcgtcacc ctgatcggcg ccaacggcgc cggcaagtcg accctgctga tgacgctctg
4621 cggctcgccg caggcggcca gcggcagcat ccgctacgaa ggcgaggaac tggtcggcct
4681 gccctcctcc accatcatgc gcaagagcat cgcggtggtc ccggaaggcc gccgggtctt
4741 ctcccgcctg acggtggaag aaaacctggc gatgggcggc ttcttcaccg acaaggacga
4801 ctaccaggtg cagatggaca aggtgctgga actcttcccg cgcctgaagg aacgctacga
4861 acagcgcgcc gggaccatgt ccggcggcga acagcagatg ctcgccatcg gccgcgcgct
4921 gatgagcaag ccaaagctgt tgctgttgga cgaaccttcg ctcggcctgg cgccgatcat
4981 catccagcag atcttcgaga tcatcgagca gttgcgtcgc gaaggcgtca ccgtgttcct
5041 cgtcgagcag aacgccaacc aggcgttgaa gctcgccgat cgcgcctacg tgctggagaa
5101 cggccggatc gtcatgcacg acaccggcgc cgccctgctg accaacccga aggtgcgcga
5161 cgcctacctc ggcggctgag caccgtcccg ccggagactt cagcgcatac gaaaacggcc
5221 ctcacgggcc gttttttatg ccgaggcgta ccggctacag gccgtagcgc tcgatagcgt
5281 ccaggtgcgg ctgggtgtcg ac
//