GenBank-Updates@genbank.bio.net (04/19/91)
LOCUS CLOCMBAB 4592 bp ds-DNA BCT 19-APR-1991
DEFINITION Clostridium thermoaceticum (thermophile) carbon monoxide
dehydrogenase alpha and beta subunit genes, complete cds.
ACCESSION M62727
KEYWORDS acetyl-CoA synthase; carbon monoxide dehydrogenase;
nickel-iron-sulfur protein.
SOURCE Clostridium thermoaceticum (thermophile) DNA.
ORGANISM Clostridium thermoaceticum
Prokaryota; Bacteria; Firmicutes; Endospore-forming rods and cocci;
Bacillaceae.
REFERENCE 1 (bases 1 to 4592)
AUTHORS Morton,T.A., Runquist,J.A., Ragsdale,S.W., Shanmugsandaram,T.,
Wood,H.G. and Ljungdahl,L.G.
TITLE The primary structure of the subunits of carbon monoxide
dehydrogenase/acetyl-CoA synthase from Clostridium thermoaceticum
as obtained by cloning and sequencing of the genes, and by
sequencing peptide fragments
JOURNAL Unpublished (1991)
STANDARD full staff_entry
FEATURES Location/Qualifiers
CDS 270..2294
/product="carbon monoxide dehydrogenase beta subunit"
/gene="carbon monoxide dehydrogenase"
/codon_start=270
CDS 2315..4504
/product="carbon monoxide dehydrogenase alpha subunit"
/gene="carbon monoxide dehydrogenase"
/codon_start=2315
BASE COUNT 1095 a 1247 c 1239 g 1011 t
ORIGIN
1 gaattcatcc tcaaccactt gccgtcaggt gaggtcatcg gccttattcc ctttcagccg
61 gcaatccttg accaggcagc cgggattacc agtacacagt cctttattgc tacaggtatt
121 actgaactgg cttcccgatt aatagagaat taaaccagat taccggggta tacggcgggg
181 ggaaccggag ttctctccgg aaaaaaatgg gtaataacac ctgttaaccc aacgggtgta
241 ggattaaatg taccaaggag gtaagcagta tgcccaggtt ccgcgatctc tcccataatt
301 gtaggccctc agaggcacca cgggtcatgg aacccaaaaa cagggaccgc accgtagatc
361 cggcggtcct ggaaatgctg gttaaaagta aggatgacaa agtcatcacc gcttttgacc
421 gcttcgtcgc ccagcaaccc cagtgtaaaa tcgggtatga aggtatttgc tgccgtttct
481 gcatggccgg tccctgccgt atcaaggcaa ccgatggccc tggcagccgt ggtatttgcg
541 gcgcttctgc ctggaccatt gtcgcccgta atgtaggttt aatgatcctt accggtgccg
601 ccgcccactg cgaacacggc aaccatatag cccatgccct ggtagaaatg gccgaaggta
661 aagctcctga ttatagcgtc aaggacgagg ccaagctcaa agaagtctgc cgacgggtgg
721 gtattgaggt agaaggcaaa agcgttttgg aactggccca ggaggtaggc gagaaggccc
781 tggaagactt ccgccgcttg aagggtgaag gtgaagccac ctggctgatg accactatta
841 atgagggccg gaaagaaaag ttccgtaccc acaatgttgt tccctttggt attcatgcct
901 ctatttccga gctggtcaat caggcccata tgggtatgga taacgaccct gttaacctgg
961 tcttcagcgc catcagggta gccctggctg actatacggg tgaacatata gctactgatt
1021 tctccgacat tctcttcggt actccccaac cggtggtcag cgaagccaac atgggggtcc
1081 tggatccgga tcaagtcaac ttcgtcctcc atggccataa tcccttgttg agtgagatta
1141 ttgtccaggc ggcgcgggag atggaaggag aggccaaggc cgccggtgcc aaaggcatca
1201 acctggtggg tatctgctgc accggtaacg aagtcctgat gcgccagggt atccccttgg
1261 ttacttcctt cgcctcccag gaactggcca tctgcaccgg agctattgac gccatgtgcg
1321 tcgacgtcca gtgtattatg ccttccatca gcgccgtagc cgagtgttat catacccgga
1381 tcatcactac tgccgataac gccaagattc ccggtgccta ccatatcgac tatcaaacgg
1441 ctacggctat cgaaagcgcg aaaaccgcca tccgcatggc catcgaggca ttcaaggaaa
1501 gaaaagaaag taaccgtccg gtttacatcc cccagattaa gaaccgggta gtcgccggct
1561 ggagccttga agccctgacc aaactcctgg ctacccagaa tgctcaaaat cccatccggg
1621 tactcaacca ggccatcctg gacggtgaac tggctggcgt agccttaatc tgcgggtgta
1681 acaacctcaa agggttccag gataactccc acctgacggt aatgaaagaa ctgctgaaaa
1741 ataatgtctt tgtggtggct acgggttgct ccgcccaggc cgccggaaag cttggcctcc
1801 tggatccggc caatgtggaa acctactgcg gcgatggtct caagggcttc ctgaaacgcc
1861 tgggtgaagg cgccaacatc gaaatcggcc tgccgcctgt gttccacatg ggttcctgtg
1921 tggataactc ccgggccgtc gacctcttga tggccatggc caacgatctg ggcgtagata
1981 ccccgaaggt gcccttcgta gcctcggccc cggaagccat gagcggtaag gctgccgcca
2041 tcggcacctg gtgggtatcc ctcggcgtac cgacccatgt cggcaccatg cccccggtag
2101 aaggtagcga cctcatttat agtattctaa cccagatagc cagcgacgtt tatggtggtt
2161 acttcatctt cgaaatggat ccccaggtag ctgcccggaa gatccttgac gccctggaat
2221 accgcacctg gaagctgggc gtacacaaag aggtagctga acgttatgaa accaaactct
2281 gccagggtta ctagaggatg aggagggtca gttaatgact gattttgata aaatcttcga
2341 gggtgctatt ccagaaggta aagagccggt agccctgttc cgggaggttt accacggcgc
2401 cattacagct accagttacg cggaaatcct tttaaaccag gccatccgga cctatggtcc
2461 cgaccatccc gtcggttatc ctgatacagc ctattacctg ccggttattc gctgtttcag
2521 cggggaagag gtcaaaaaac tgggggattt accacctatt ttaaaccgca agcgagcgca
2581 ggtaagccct gtcctgaatt tcgagaatgc ccgcctggcc ggggaagcca cctggtatgc
2641 ggccgagatc attgaagccc tgcgttacct taaatataag cctgatgaac ccctcctgcc
2701 cccaccctgg acgggtttca tcggcgaccc ggttgtccgc cgtttcggta tcaagatggt
2761 cgactggacc attccgggtg aagctattat cctgggtcga gccaaagact cgaaggccct
2821 ggccaaaatc gtcaaggaac tcatgggtat gggctttatg ctcttcatct gtgatgaagc
2881 ggtagaacag ctgctggaag aaaacgtcaa actggggatt gactatatcg cctatcccct
2941 ggggaacttc acccagattg ttcatgccgc caactatgcc ctgcgggctg gtatgatgtt
3001 cggtggcgtt accccgggtg cccgtgaaga acagcgcgat taccagcgcc gccgtatccg
3061 cgcctttgtc ctttatctcg gcgagcatga catggtcaag acggctgccg ccttcggggc
3121 catctttacc ggctttccgg taatcaccga ccagccccta ccggaggaca aacagatccc
3181 ggattggttc ttcagcgttg aggactatga taaaatagtc cagatagcca tggagacccg
3241 tgggatcaag ctcaccaaga tcaagttgga tctgcccatt aactttggcc ctgcctttga
3301 gggcgagagt atccgtaagg gcgatatgta cgtagaaatg ggcggcaacc ggacgccggc
3361 ctttgagctg gtacgcaccg tttcggaatc cgagatcact gatggtaaga ttgaagtcat
3421 aggtcctgat attgaccaga taccggaagg gagcaaactg cccctgggca ttctggtgga
3481 catctatggc cgtaaaatgc aggccgattt tgaaggagtc ctcgaacggc gcatccacga
3541 cttcatcaac tacggtgaag gtctctggca caccggccag cgtaacatca actggttgcg
3601 ggtcagcaaa gatgccgtag ccaagggttt ccgtttcaag aactacggtg aaatcctggt
3661 agccaaaatg aaagaagaat tccccgccat tgtggaccgg gtccaggtaa ccatttttac
3721 cgatgaagcc aaggtcaaag aatatatgga ggtcgcccgg gagaaataca aggaacgtga
3781 cgaccgcatg cgcggcctta ccgatgaaac agtggatacc ttttactcct gcgtcctctg
3841 ccagtccttt gcccccaacc atgtgtgtat tgtcaccccg gaacgggtgg gcctgtgtgg
3901 agccgtaagc tggctggacg ccaaggcgtc ctatgaaatc aaccatgccg gtcctaacca
3961 gcccatccct aaagaagggg aaattgatcc cattaagggt atctggaaga gtgtaaatga
4021 ctatctctat acagcttcca accgtaacct ggaacaggtc tgcctgtaca cccttatgga
4081 gaatcccatg acctcctgcg gttgctttga ggccattatg gccatcctgc cggagtgcaa
4141 cggcatcatg attaccacca gggatcacgc cggcatgact ccttcgggga tgaccttctc
4201 taccctggcc gggatgatcg gcggtggcac ccagaccccg ggctttatgg gcatcggccg
4261 cacctatatc gtcagcaaaa agtttatttc cgccgatggt ggtatcgccc ggatcgtctg
4321 gatgcccaaa tctctgaagg atttcctcca cgacgagttt gtacgtcgta gtgttgagga
4381 gggcctggga gaggacttta tcgataaaat agctgatgag accatcggta ccaccgtgga
4441 tgaaatcttg ccctacttgg aggaaaaggg acacccggcc ttgaccatgg atcccattat
4501 gtgagagcct gcacccggtt ccggggctat cccggggatt ggccccggaa ccgagctgaa
4561 ttaatcatga aagaaaggag tcaacaaata tg
//